Okolab H301-ALPHA3-SMALL User manual

H301-ALPHA3-SMALL User Manual
REV 02
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H301-ALPHA3-SMALL
Compatible with the following microscope
Compatible with the following Okolab Controllers
•PhaseView Alpha3Light Sheet microscope
•Bold Line
•UNO
IST-2016 _ REV01

H301-ALPHA3-SMALL User Manual
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Index
1. Components and dimensions................................................................................................... 3
2. Insertion of the Sample Feedback Temperature Sensor.................................................................. 4
3. Connection of the Gas Supply ................................................................................................. 4
4. Working with perfusion ......................................................................................................... 5
5. Installing the H301-ALPHA3-SMALL around the sample holder.......................................................... 6

H301-ALPHA3-SMALL User Manual
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1. Components and dimensions
H301-ALPHA3-SMALL includes the following components:
•Objective Collar, compatible with water immersion objective 21 mm diameter.
•Sample Arm Cover
•Chamber Lid
•Chamber Base
Chamber Base
Objective
Collar
Chamber Lid
Sample Arm
Cover
Figure 1. H301-ALPHA3-SMALL –Components and dimensions.

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2. Insertion of the Sample Feedback Temperature Sensor
Insert the Sample Feedback Temperature Sensor through the dedicated opening located in the H301-ALPHA3-SMALL
(see Figure 2).
To read the temperature of the culture medium and realize a self-calibration of the chamber, fix the Temperature
Sensor tip with adhesive tape on the sample holder inside the H301-ALPHA3-SMALL. For more information about the
self-calibration, please refer to the User Manual of the Okolab Controller in use.
Figure 2. Insertion of the temperature sensor inside the chamber.
3. Connection of the Gas Supply
A single silicon tubing carries output gas from the Okolab Gas Controller to H301-ALPHA3-SMALL. Silicon tubing con-
nects to a gas input - brass opening - located on a corner of the H301-ALPHA3-SMALL (see Figure 3). Connect by gently
pushing silicon tubing onto brass opening.
Gas Input
Figure 3.Connection with gas supply.

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4. Working with perfusion
The Chamber H301-ALPHA3-SMALL features 2 perfusion holes on the sides for the insertion of perfusion tubing up to 1
mm in outer diameter. Follow the instruction below to work with perfusion:
1. Use the supplied Stainless Steel Perfusion Tubes. Attach a Silicon tubes O.D. 1,6mm I.D. 0,8mm (not supplied) to
the Stainless Steel Perfusion Tubes (see image 1 in Figure 4).
2. Insert the Stainless Steel Perfusion Tubes in the perfusion holes (see images 2 and 3 in Figure 4).
Insert a Silicon tubes O.D. 1,6mm I.D. 0,8mm (not supplied) to the free end of the Stainless Steel Perfusion Tubes
(see image 4 and 5 in Figure 4).
Figure 4. Working with Perfusion

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5. Installing the H301-ALPHA3-SMALL around the sample
holder
To install the H301-ALPHA3-SMALL on the stage, please follow the steps below:
3. Insert the chamber base by sliding into place as shown in image 1 and 2 in Figure 5.
Note: Please pay attention to the sample holder glass.
4. Tighten the captive screw M2 to fix the chamber base around the sample holder by using the provided hexagonal
key (see image 2 in Figure 5).
5. Place the magnetic lid on the chamber base (see image 3 in Figure 5).
6. Insert the sample arm cover (see image 4 in Figure 5), and close it (see image 5 in Figure 5). It allows to reduce
the brightness into the sample.
7. Place the magnetic objective heater in place as shown in image 6 in Figure 5.

H301-ALPHA3-SMALL User Manual
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5
4
2
1
3
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6
Figure 5. Connection of the Chamber with Stage
H301-ALPHA3-SMALL_User Manual Rev 02_Published in September 2020