Okolab H301-MIZAR-TILT User manual

H301-MIZAR-TILT User Manual
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H301-MIZAR-TILT
Compatible with
Compatible with the following Okolab Controllers
•Mizar Tilt system
•UNO-T
•UNO-T-H-PREMIXED
•UNO-T-H-CO2
•H301-T-UNIT-BL-PLUS
•Bold Line Gas controllers
IST1748_REV02

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Index
1. Components and dimensions................................................................................................... 3
2. Available Light Trap ............................................................................................................. 4
3. Available Magnetic Clamp ...................................................................................................... 4
4. Optional Components ........................................................................................................... 5
4.1 Available Sample Holders ........................................................................................................ 5
4.2 Available Lid........................................................................................................................ 5
5. Insertion of the Sample Feedback Temperature Sensor.................................................................. 6
6. Working with OKO-MH Sample Holder........................................................................................ 7
7. Working with 1”x3’’ chamber slide .......................................................................................... 9
8. Working with 35mm petri-dish ...............................................................................................11
9. Working with MIZAR-CUBE GL.................................................................................................13
10. Connection of the Gas Supply .............................................................................................13
11. Working with perfusion.....................................................................................................14
12. Connection of the Chamber with Z stage ...............................................................................14
13. Cleaning .......................................................................................................................15

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1. Components and dimensions
H301-MIZAR-TILT includes the following components:
•Chamber Lid with embedded temperature sensor
•Light Trap to avoid any reflected light
•Magnetic Clamps to snap lock the chamber slide
•Chamber base with embedded temperature sensor
•Chamber Bracket to fix the chamber to the stage
Distancebetween focal plane and
upper side of the glass lid
Chamber top view
Chamber Lid
Light Trap
Magnetic Clamp
Chamber Base
Chamber Bracket
Figure 1. H301-MIZAR-TILT - Components and Dimensions.

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2. Available Light Trap
H301-MIZAR-TILT can accept Mizar Cube Holder (with or without guide arm) and 1”x3” slides/chamber slide. H301-
MIZAR-TILT must be equipped with at least one light trap. The following light traps are available for H301-MIZAR-TILT.
LIGHT-TRAP-MCH
MIZAR-1x22by40
Light trap for Mizar Cube Holder (with or without guide arm).
LIGHT-TRAP-GS
Light trap for 1”x3” slides/chamber slides.
LIGHT-TRAP-MCH LIGHT-TRAP-GS
Figure 2. Available Light Trap
3. Available Magnetic Clamp
H301-MIZAR-TILT can accept standard glass slide with thickness between 1.50 up to 2 mm and also glass slide with
thickness between 2.1 to 4 mm. The following magnetic clamps are available for H301-MIZAR-TILT.
MAG-CLAMP-1.5-2
Magnetic clamp for slides/chamber slides with thickness in the range 1.50 –2 mm.
MAG-CLAMP-2.1-4
Magnetic clamp for slides/chamber slides with thickness in the range 2.10 –4 mm.
Figure 3. Available Magnetic Clamps

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4. Optional Components
4.1 Available Sample Holders
The following sample holders are available for H301-MIZAR-TILT.
NOTE: Please contact [email protected] if you cannot find the sample holder you are looking for. We are constantly
adding new inserts to the list.
MIZAR-1x35
Holder for 35mm petri-dish.
MIZAR-1x22by40
Holder for 22x40 mm coverslip
MIZAR-1X35 MIZAR-1x22by40
Figure 4.MIZAR-1X35 and MIZAR-1x22by40 Sample holder
4.2 Available Accessories
MIZAR-CUBE GL
Stainless Steel Top Lid for 12.50x12.50 Mizar Imaging Cube. It helps to minimize
evaporation.
Figure 5. MIZAR-CUBE GL

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5. Insertion of the Sample Feedback Temperature Sensor
Insert the Sample Feedback Temperature Sensor through the dedicated opening located in the H301-MIZAR-TILT (see 1
in Figure 6).
To read the medium temperature and realize a self-calibration of the chamber, fix the Temperature Sensor tip with
adhesive tape on the bottom of the medium volume of the sample holder. For more information about the self-
calibration, please refer to the User Manual of the Okolab Controller in use.
Sensor Tip
AdhesiveTape
Opening for Temperature Sensor
1. Frontal view 2. Temperature Sensor Placement
Figure 6. Insertion of the temperature sensor inside the chamber

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6. Working with MIZAR-1x22by40 Sample Holder
The OKO-MH fits into the Chamber Base and it is held in place by the magnetic clamps in order to prevent movement
inside the opening on the base. To insert the MIZAR-1x22by40, follow the steps shown in Figure 8 and listed below:
1. Insert the MIZAR-1x22by40 into the Chamber Base (see image 1 in Figure 8).
2. Place the magnetic clamps over the insert (see image 2 in Figure 8), then push down the head of the clamps sim-
ultaneously and move it in the direction shown in the image 3 of Figure 8.
3. Insert the Light Trap into the Chamber Base as shown in image 4 in Figure 8.
4. Close the Chamber Base with the Lid (see image 5 and 6 in Figure 8).
NOTE: Please follow the same steps also to insert the Mizar Cube Holder.
Figure 7. Base Top view.

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Figure 8. Working with MIZAR-1x22by40 Holder.

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7. Working with 1”x3’’ chamber slide
The 1”x3” chamber slide fits into the Chamber Base and it is held in place by the magnetic clamps in order to prevent
movement inside the opening on the base. To insert the 1”x3” chamber slide, follow the steps shown in Figure 10 and
listed below:
1. Insert the 1”x3” chamber slide into the Chamber Base (see image 1 in Figure 10).
2. Place the magnetic clamps over the 1”x3” chamber slide (see image 2 in Figure 10), then push down the head of
the clamps simultaneously and move it in the direction shown in the image 3 of Figure 10.
3. Insert the Light Trap into the Chamber Base as shown in image 4 in Figure 10.
4. Close the Chamber Base with the Lid (see image 5 and 6 in Figure 10).
Figure 9. Base Top view.

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Figure 10. Working with 1'' x 3'' chamber slide.

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8. Working with 35mm petri-dish
Flat springs prevent movement of 35mm petri-dish inside the opening on the Chamber Base. To insert the MIZAR-1x35,
follow the steps shown in Figure 12 and listed below:
1. Insert the MIZAR-1x35 into the Chamber Base (see image 1 in Figure 12).
2. Place the magnetic clamps over the 1 MIZAR-1x35 (see image 2 in Figure 12), then push down the head of the
clamps simultaneously and move it in the direction shown in the image 3 of Figure 12.
3. Turn the flat springs (see image 4 in Figure 12) and insert the petri-dish (see image 5 in Figure 12) into the sample
holder.
4. Lock the petri-dish by using the flat springs (see image 6 in Figure 12).
5. Close the Chamber Base with the Lid (see image 7 and 8 in Figure 12).
Figure 11. Base Top view.

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Figure 12. Working with 35mm petri-dish

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9. Working with MIZAR-CUBE GL
H301-MIZAR-TILT can accept Mizar Glass Holder with Mizar Imaging Cube. The following glass lid is available to cover
the 12.50 x 12.50 mm Mizar Imaging Cube, in order to reduce evaporation of the medium. The glass lid provides a lat-
eral dedicated hole to insert the Sample Feedback Temperature Sensor (see 2 in Figure 13).
Temperature Sensor dedicated opening
1. Insertion of MIZAR-CUBE GL 2. Temperature Sensor
Figure 13. Working with MIZAR-CUBE GL
10. Connection of the Gas Supply
A single silicon tubing carries output gas from the Okolab Gas Controller to the H301-MIZAR-TILT. Silicon tubing con-
nects to a gas input - brass opening - located on rear side of the H301-MIZAR-TILT. See Figure 14. Connect by gently
pushing silicon tubing onto brass opening.
Gas Input
Figure 14.Connection of the gas supply.

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11. Working with perfusion
H301-MIZAR-TILT features 4 perfusion holes on the sides of the chamber base for the insertion of perfusion tubing up
to 2.0 mm in outer diameter. Small screws plug the perfusion holes when not in use (Grub screws M2,5x3). Remove
the small screws as necessary before introducing perfusion tubing.
Figure 15 shows the location of the perfusion holes.
Openings for
Perfusion
1. Frontal view 2. 3D view
Figure 15. Perfusion
12. Connection of the Chamber with Z stage
To fix the H301-MIZAR-TILT on the stage, follow the steps listed below and shown in Figure 16:
1. Fix the bracket to the SLR.300 Piezoconcept Stage with four M2.5x6 screws (see Image 1 of Figure 16).
2. Fix the base of the chamber with two M3x6 screws on the bracket (see Image 2 of Figure 16).
3. Insert the sample holder (see paragraph 5, 6 or 7 according to the sample holder you have purchased).
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Figure 16. Connection of the Chamber with Z Stage

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13. Cleaning
•Turn the system off and pull the mains plug out the socket
•Remove the lid from the chamber main body, and keep it separate from the chamber main body while the
chamber cools down.
•To clean the body and the glass lid of the chamber, wipe with a soft micro-fiber cloth. For stubborn smudges,
you can damp the soft micro-fiber cloth with ethyl alcohol (product code UN1170). Do not put any liquid di-
rectly on the chamber. While cleaning the glass lid, do not apply strong force to the surface of the glass lid
because it can be damaged.
H301-MIZAR-TILT_User Manual Rev 02_Published in February 2020