Olympus CK2 User manual

MODELCK 2
INVERTED MICROSCOPE
.------WARNING -------,
This instruction manual is for use of the Inverted Micro-
scope Model CK2. Before
putting the microscope into
operation, we recommend you read this manual carefully
in order (0 familiarize yourself fully with the use of this
micrO$Cope so that you may obtain optimum performance.

BEFORE USE
D Operait,",on!ii:===~
I A$ iiJ microscope is; aprecision innrument, always handle it with the care it deserves and avoid abrupt
motions and 5hocb.
2 When you retnO\le the mICroscope Irom the styrofOilm CCI5f!,
hold the stage legs at Aand B (Fill 11
3 For mcwemefll of the microscope, hold the stage leg A
and lhe observation lube C Do nol hold the lamp hous-
ing pillar. (Fig. 11
4The light source incorPOriltflS a 6 V 20W halogen bulb.
00 not use any olher bulb.
5Avoid expO$Ure of the microscope to dirK! sunlight, high
temperature and humidity, dUSI and vibration.
00 Before use, ascertain thaI the line voltage selector switch
at the base plate of the microscope Stand is SIlt in con-
formity wIth the local line Yoltage.
.. Specifications of the eleetrical components differ from
others than the equipment with 12QV of supply circuit,
aI the eq.,ipment is in compliance with the reQUirements
of Underwriull Labor"o,i~. Also, the equipment is
not needed to $~ect volt •.
7Before fuse replacement, dIsconnect the pawer cord from
the AC outlet. The fuse boK 1 IS Iocilted at the back
of the micrOSCOPe stand. IF'9 21
Use ascre.vdnYllJ 10 remove this boK
• Always Qfound the micrOSCOPe.
A
Fig 1
Fig 2
:s'
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II Maintenance and sto~
CD lens surfaces must ajways be kept clean F,ne dust on lens surfaces should be blOWll off by means of Mild
blower Carefully Wipe off 011 or flngerpnnts on the lens surfaces with gauze ffiOlstened WIth a small
amount of alcohol and ether (3 7) m1lrture, or xylene
Do
not use organiC solutions to wipe the surfaces of vanous components Plastic parts. especlllily. should
be cleaned With aneutral detergent
@Be careful not to spill the culture solution, etc. If spilled. It should be Wiped off immediately.
® When objectives are not screwed Into the nosepiece apertures. seal the apertures With dust plugs, which
Will protect
the lenses located In the lower light path from dust. culture solution, etc.
@ When not In use, the microscope should be covered WIth the dust cover provided.
@AtenSion adjustment ring CD IS provided neKt to the coarse
focus adjustment knobs With thiS deVIce the tenSion of the
coarse adjustment IS freely adjustable lor elther heavy Of IlQht
movement. depending upon operator prelerence (Fig 3)
Applying the tiP of a large screwdriver at a groove In the
penphery 01 the tenSIOn adjustment nng CD. rotate the flng In
the d,rectlon of the arrow to Increase the tensIOn. Of feverse
the flng to loosen,
However, do not loosen the lenslOfl adJustment nng tOO much.
because thiS may cause the line locus adjustment knobs to
slip. Fig 3

CONTENTS
,.
SPECIFICATIONS I
2. STANDARD CONFIGURATIONS
22
3. NOMENCLATURE
3
:&
4.
OPERATION
4I
4-1
Assembly and Operation of Each Component 4
I
4-2
Phase Contrast Observation 7
5. PHOTOMICROGRAPHY
8-
.J
5-1 Photomicrographic System and Optional Accessories 8
6.
TROUBLESHOOTING GUIDE
9
f.

I
SPECIFICATIONS
D
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Des:rjptiOn
Focus adlustment by vertical movemel'll of the nosepiece (stage is tilled) by mNtIS of
Focusadjustment
the OOltfw/fine adjustment knobs. provided With tension adju$lmel'll mechanism.
Foo.aing range lfrom lhe focal point 00 lhe stage surflcel: 6mm upward arod 21TWTl
down ... ",
--
Quadruple, with provision for mountingasimple lilter analyzer.
S_ 160 mm (XI X 240mm (Y). Substage: 70mm IXI x
180 mm (V).
Anilchilble Traversing area 120 mm (XI x78 mm (Vl; coaxial low drive control knobl on the
rnechiJ~ical stage fIQht or left 'ide of the plain stage; provided with 2culture vessel holders
Light source 6 V 20 Whalogen
bulb
Light intensity control Continuously variable by resistor
Illumination F
iller holder For 45 mm-dia. filters.
Built-in filter Frosted.
heat absorbIng filter
Condenser Ultra Iong-work.ing distance condenser
NA.
0.3.
W.O. 72 mm; detaChable.
Optics
for observation Afacal
optical system.
Inclined 45-; interpupillary distance adjustment
range 53 through 72 mm; With adi-
Binocular
lube
opter IIdlUstment helicoid on tl'le left 51_
Inclined 45-; Interpupillafy distance adjustment range 53 through nmm; WIth d,opter
Trinocular tube IIdjustment helicoid
on both sleeves..
Provided WIth aa:ommodiU'on lor 3annuli, ioc:tuding one annulus for lOX (oJ,1K) U$Ild
Lig,t annulus slider
lor 2OX) OOJ8C11Ye and twO anouli lor 4X and 4QX objectives; detachable
PC S Plan 4XPl NA. 0.13; W.O. 15.5 mm
PC Dl0XPL NAO.25; w.O. 7.2 mm
For phase COtllfast
PC LWD CD 20XPL NAO,40; W.O. 5.4 mm
PC LWD COPlan 4QXPL
NAO.60; W.O, 1.9mm
Dbl8Ctiv@s
(correction collar)
D4X NAO.lO; W.O. 18.2 mm
For bright field
Dl0X NAO.25; W.D. 7.2mm
LWD CD20X
N.A.0.40; W.O. 5.4 mm
Dimensions 200 mm
(W) X 340 mm (D) X469 mm (HI
Evepoint height
Bil"lOCUlar lube 342 mm (13.5 in.). TrinOOJlar tube
332mm (13.3in.1
Weighl
About 7 k.g (15.4Ib.1

2.
STANDARD CONFIGURATIONS
...... ,CK2·BIP CK2-81C
CK2·TRP
CI(l·lRe
ComPQtlent
-,
,,
-,
,
-,
-\ ,
Microscope stand with lil<ed
binocular lube. including CK2Bl·F 0 0 0 0
CK2-U LWCD cooclenllef
M,croscope stand. includir'lQ
CK2TR·F
0 0 0 0
CK2·ULWCD condenser
Trinouclar lUbe BH·TR45·W 0 0 0 0
SliIQe eXlenS;Qf1 plates
CK2·SS
0 0 00
(2~1
000
"~,
(211U) (2PC:f!
Attachable med'lllnical stage CK2-MVR
0 0 0 0
Halogen lamp socke! LS20HM 00000000
Halogen bulbs 12 pa I 6V2ONHAl
00000000
~cord
uycp
00000000
\)4X
0000
DIOX
0 0 0 0
PCSPlan 4XPL 0 0 0 0
Objectives
PC010XPL
0 0 0 0
LWDCD20XPL
0 0 0 0
lWOCD20X 0 0
LWQCDPlan4QXPL 0 0
Phase slider" CK2·SL 0000
Phaseanoular ring for CK2·RS40 0 0
40X objective
LB (Bltering te!elcoPe
CT~
0 0 0 0
LB eyeplec:* lOX 12 P(1) CWHK10X
00000000
LB photo eyepiece 5X
NFK5XLD
0 0 0 0
Fitter set·'
PM·FIL-7 0 0 0 0
Note 0indicates the compatible componems for each model.
'With annul, lor <IX and 10l2OX obJeCtives • "Incl. 45LBD2N &43ND25-W45 filters
Ell
Optional Accessories
Attachable mechanical stage
Db"ective
E , ....
(For exclusive ose w,th CK2·TRP/-TACI
omicrograptoiC sy1tem
Ma\Jn,latl()fl changer
O~ing atachment
IMT2-MVR
PC ULWOCO Plan 4QXPL
WHK1QX&WHKI5X
-,
BH2-eA
BHl.QA

3
NOMENCLATURE
II
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CK2-BIP/-BIC
Bulb socket
Phase slider
Ultra long-working
distance condenser
lamp housing pillar
Petri dish
holder
Accommodates a 35mm.{jia.
petri dish.
Stage extension plate
Objoctive
NO$epiece
Power cord receptacle
Incorporatl!s(I fuse.
Fine adjustment knob
Coarse adjustment knob
Attachable mechanical
" ...
Provided with 2specimen
holders.
Specimen holder
2types of specimen holder
are available: Terasaki holder
and slide
glass holder.
Filter holder
(for 45mm·dia. filter)
Use the cobalt filter (45Cllor
brightfield,and thegreen filter
(IF550l for phase contrast
(both filters provided).
Aperture iris diaphragm
can be stOpped down for
easier focusing on an
un·
stained specimen, or opooed
up for phase contrast.
Eyepiece
Diopter adjustmant ring
Use thiS ring for correction of
dioptric difference between
both eyes.
Binocular tube
Bulb voltage adjustmenl knob
Use of the bulb at voltage
lower than rated prolongs its
expected service life.
Main switch
Photo tube
Eyepiece
Tube length ll<:Ijustment
rings
Trinocular tube IBH·TR45·W)

4
OPERATION
* II the halogen bulb burnl out dJring operation, w.it until the
defective bulb and socket Ir. completely cooled before bulb reo
plac:em.nt to prltYlnl •burn.
21 lmen the OOnlaa pins' Icompletely into thesocket 1, (Fig. 51
.. NeYel' use excessi .... force 10 twist or bend the bulb, but IIftltly
push in the pirn,
.. 00 not lea.... fingefl)rinu, 1WtC. on the mirror surfac. , . II
Rnudged, it should be wiped with gauu moinened with t smtll
tmount of t1cohol tnd~ ... 13:11 mixture. (Fig. 5)
3) Looking at the bulb from its froot direction, ascertain thilt the
lower- half of the iICl'"ew head 00 the mirror surface is coY8l"ed
by the filament. (Fig. 61
'* If the filtment devilles horizonttlly from the center of the screw
head, the bulb tilU to the right or left. Attach the bulb again
perpendiCl.llerly.
(FiG- 6)
'* If the filament devilles vertically, the contact pim tre not in-
serted
completely. Pu~ in the pins completely. (Fig. 6)
11 P\J1I 001 the contact pins of the halogen bulb lrem the polyethylene
....
Be certain not to love fingerprinu or smudge5 on tta bulb.
aSet 1in«.¥Cll Je1-aor_i
Set the line vollage "ector PNllch <D located al the base plate 01
the mjqO$COpe n¥ld 10 the loc:al line voltage IF'll 4 shows the
setting 01 the _itch at l00V),
fJ Inna
Hi
!1ll. the h,llOgl!!n ulb
,
_ .. For Ihe U.S.:
Frames sold in the U.S. meellhe UL standard end 8fe selat
115V. Therefor, Ihis selector switch is not included.
Fig 4a
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Fig 5
,
Polyethylene bag
~
Correct Deviated
(homonlally) (venically)
• - I Assembly and Operation of Each Component
Fig 6
,
II AttIC 1118 the bulb socbt
Aligning lhe guide pins <D to lhe guide holes <I> of the lamp houSing,
slowly insert
the bulb socket completely without tilling. (Fig 7)

II Connectlog the mechanical Slage
Screw the mecharucal stilge to the right or left side of the plainstage
with clamping screws IprOl/ided II lhe underside of lhe stage,
Ti\tlten wilh iI coin. (Fig. 9)
21 Connecting the stage tKtellSlOn Platll$
The extension plales can be allached 10 lhe plaIn stage by means
of the clamping screws 2in the same manner as lhe mechanical
''''''.
II Mounti the trinocul,,'J.'.".""' _
The trillOClJlar lube can be mounted by loosening the observation
tub!l clamping knob and insening
the circular dOl/etail under lhe
tube into the microscope stand; then tighten the knob again.
QMoontmg "'h'.~ob_~· ~.;n;;::::::::::::::::J
SCrew each objective inlO the nosepiece 1Irom 1(Mt power 10
hi\tl power. rotating the nosepiece in I dockwlse d.rection, IFIlJ.
81
.. Please note that the front lens of the obj&ctive in thl inverted
microscope faces upward, and
is exposed to contamr~ion
more than the objective of an upright microscope. Therefore,
clean the front lens occasionally, and if th.re are empty open·
ings in the nosepiece, use the dust plugs prO\lided to prevent
dust or debris hom IllIing into the nosepiece.
D Anaching the mechanical st~ or ext_ion P.:,' •• "' ..... ~_
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Fig 8
oInserting
the eyepieces
Fig 10
Fig 11
Insert the eyep.eces into the observatiOn tube
lnterpupilliry distance 8dju~.~m~~~'~==:::::::::J
Hold the k,....rled sliding grips <D of the fight and left eyep.ece
sleevll$ With both haods and push the tubes together or pull them
apart laterally whIle kicking through the evepieees with both
eyes. until perfect bll'lOCUlar vision is obtaIned If you kn()N your
Interpupillary distance setllrlQ. set il ill the scale <Z) between the
eyepiece
sleeves. IFlg 10)
Diopter adjustment
1) Binocular tube
Look at the Image through lhe right eyepiece with your right
eye and focus on the specimen WIth the locus knobs. Next. look
ing at the image through the left eyeplElOe With your left eve.
rOtate the dio';lIer adjustment riog s 10 locus on the spec;.mert
without using the locus knobs. (F'9_ 10)
21 Trinocul. tube
After interpupIllary distance adjustment ,n step D. rotate the tube
length adjustment (1l'1Q I on the right eyepIeCe sleeve to match
your interpupillary distance setting which you obtained lI'om the
scale. Looklog at the image throu\tl the right eyepIece. locus on
lhe specimen by ffi8a1"l$ 01 the focus knobs, Next. looking Ihrough
the left eyepIece, rotate the tube length adjuSllTll!f1t ring 2 to
locus on the specimen By these adjustments, partocahlY can be
maintained when oblectives
are changed. with touch-up adjust·
"" .. ", ,1 ... _ ... ,,, 11=." 111

Il!I
U5B of the mechanical s~
Fig 14
Fig 12
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II
Fig 13
lAs seen through eyepiece tube,
with eyepiece removed}
11 ATetasaki holdef Iis oplJOnally available to hold aTetasaki plate
Of a 65mm-dia. petri dish. while an optional slide glass hoidel' 2 can
be used to hold a 1" x 3" slide glass Of a54mm-dia. petri dIsh on
the stage IFig. 121
21 A 96-wel1 micro-titre plate Of a24-we1l micro-titre plate can be
directly mounted on the stage. [Fig 121
*The 35 mm-di •. petri dish holder @provided is used to prevent
aspecimen veu&! from falling through the opening in the plein
stage. (Fig. 121
mAperture iris diaphragm
An aperture iris diaphragm buill in the illuminator is used to limit
lhe angular aPerture of an objecllVll in brightfjeld. and controllable
10 oblain optimum obitctiVll perforfT\itf"lOll, depending upon focal
IengIh.
contrast and r8lOlution. The ope'llng of IN! aperture ins
diaphragm should be matched to the N.A of the objecti..e in u •.
It is often preferable, however, 10 Stop down lhe apertUre d .. ·
pl'lfagm to about 70% through 80% of the ootectlVe NA IF'O 131
* An unsuined specimen can be more nsily observed with a
stopped-down aperture in bright filtld.
If) Sening of the correction collar
An Inverted microscope is conveniently arranged
to observe
weeimem contained In vessels of various bonom thicknesses. In
order 10 al;hieve optimum obiectiVll performance of lhe inverted
micrOSCoPe, the high N.A. objectives are provided with a correction
collar <D. [Fig 14)
The correction collar is effective with aveueI bottom from 0to
2mm thickness.
11 If the thickness of the vessel bottom is known.
Match the CO'rection collar to tM thickness of the vessel bollom
by the collar scal. provided
2) " lhe thickness of the _I bollom Is unknown:
The OPtimum POSition for the correction collar can be obta,....:l
from the image resolution. After focus adjustment, if a satisfac·
tory sharp image is not obtained, rotate 1M correction collar to
the right and left 10 lnat you can compare the imagll!i at both
sides. Reset lhe collar to tnt bener Image, then S1arting from
lhis position, further rOtate the collar to the right and left until
both images can be Obtained for comparison with each Other.
As you repeat Ihis procedure several times, you can find besl
position for the correction collar, Refocus after rotaling the correc·
tion collar.
lEI Filters ...:===:.. __ ~
Optimum selection of filters enhances the elleaive performance in observati(lfl and photomicrography,
No
Filter
Pu""""
1InterlerfnO! 19reenl 4S.IF550
Enh¥Fces Pl'lam CX)l'ltrast ifn9S.
2Neutral
d."ity (gray) 43ND2S-W45 Reduces hght intensity w,thout ctFaog ngspect .... 1
characteristics of the light 3OUrce.
3Light balancing 4S.LBD-2N For color photomicrography With daylight film.
,Heat abtorbing 45·HAo Absorbs heat waves from Ihe light
KlUrCl 10 pro·
teet tnt specimen.
*This f,lt.r IS bu,lt In the CK2. It IS recommended to add. second heat absorbing fllt.r for prolonged

I~' 2Phase Contrast Observation I
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Legend comes atop.
00
Legend comes atop,
Fig 15
@-@
Fig 18
DPre aration of the philSe slider
Put the li!tlt annuli for 4X ill and 40X <V objectives inlO the phase
slider. The annulus@ for lOX is also use<! for 20X objectives. The
annuli for 4X and lOX {lOX Iobjectives are centered by means of
the centering lever@, (Fig. 15)
Mount the light annulus <V for 40X objective into the center hole
of the phase slider (as the ARROW indicates).
fJ l:'ko~ .. ~rti:i~O~O~flJjh~.~p~h~LiIT;'~;:============::::l
Insert the phase slider <D into the slot in the condenser in such a
manner that its engraved surface with figures faces upward. and
the notched side toward the lamp housing pillar. Rotate the aper-
ture iris diaphragm lever <V all the way to the left, and insen the
green filter provided intO the filter mount (j). (Fig. 16)
II Cantratl 0 tha ;tu
1) Place aspecimen on the st~ and focus on it.
Remove one of the eyepieces from the observation tube and insert
the centering telescope CT·5 CD provided into the observation
tube. {Fig. 17)
Ascertain that objective magnification and annular ring are matched.
Rotate the top lens assembly of the centering telescope to bring
the bright ring (light annulus) and the dark ring (phase annulus)
into focus.
Operate the centering lever of the light annulus until both annuli
are concentric and superimposed. (Fig. 181
* If aspecimen slide or the bottom surface of Icufture ve$Sill is
not flat. either annulus is likely to slip off the center while
centering the other. In such a caSIl. repeat centering adjustment
again. This trouble, however, rarefy occurs with hi!tler magnifica·
tions, so that the 40X philSe annulus needs no centering adjust·
ment.
21 After centering adjustment with the lOX objective, check the
centration with the 20X objective.
3} After centration is complete, remove the CT.s and insert the eye·
piece back into the observation tube.
Now it is ready for phase contrast observation.