Olympus BHTU User manual

MODELBHTU
BIOLOGICAL MICROSCOPE
scanned on Nov. 6, 2009 by J.G.McHone
for personal use only, not for sale
,.---- WARNING ------,
This instruction manual is for use of your instrument.
Before
putting it into operation, we recommend you
read this manual carefully in order to familiarize your-
self fUlly with its use so that you may obtain opti-
mum performance.
OLYMPUS'"
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AX5?51 .

This instruction manual has been written for the use of the Olympus Biological Microscope
Model
BHTU. It is recommended that you read the manual carefully in order to familiarize
yourself
fully with the use of the microscope, so that you will obtain optimum performance.
IMPORTANT
Observe the following points:
•Operation
1. Always handle the microscope with the care it deserves, and avoid abrupt motions.
2. Avoid the use and maintenance of the microscope in direct sunlight, high temperature and
humidity, dust and vibration.
3. Only use the tension adjustment ring for altering the tension of the coarse adjustment
knobs. (Do
not twist the two coarse adjustment knobs in opposite directions simultaneous-
Iy, as this will cause damage.)
4. Make sure that the voltage selector switch on the bottom base of the microscope stand
is set to conform with the local mains voltage.
5. Ground the microscope in case there is no ground terminal in your mains line .
•Maintenance
,. Lenses must always be kept clean. Carefully wipe off oil or fingerprints deposited on the
lens surfaces
with gauze moistened with asmall amount of xylene, alcohol or ether.
2. Do not use organic solutions to wipe the surfaces of various components. Plastic parts,
especially, should
be cleaned with neutral detergent.
3. Never disassemble the microscope for repair. Only authorized Olympus service personnel
should make repairs.
4. The microscope should be covered with the vinyl dust cover provided and stored in aplace
free from humidity and fungi.

I.
II.
III.
STANDARD CONFIGURATIONS
NOMENCLATURE
ASSEMBLy............ .....
CONTENTS
2
3
4
IV. IDENTIFICATION AND FUNCTION OF VARIOUS COMPONENTS
V.
VI.
VII.
OPERATION
A. Switching ON the Light Source
IVoltage Adjustment and Light Intensity I
I
LP (Light Intensity Presetting) Switch I
B. Placement of aSpecimen Slide .
IWhite Cover for Condenser
ICover Glass I
ISpecimen Slide I
IStage I
C. Observation Tube
1. Interpupillary Distance Adjustment
2. Diopter Adjustment
3. Light Path Selector
D. Condenser Adjustment
1. Condenser Centration
IFiled Iris Diaphragm I
IAperture Iris Diaphragm
E. Focusing Adjustment .
1. Tension of Coarse Adjustment Knobs and Fine Adjustment
IUse of Rubber Cap for Fine Adjustment Knob
2. Pre-Focusing Lever
F. Use of Immersion Objectives
G. Photomicrography .
OPTICAL DATA
TROUBLESHOOTING GUIDE
6
9
9
10
13
14
15
15
17
18

I. STANDARD CONFIGURATIONS
"",
Model
Component BHTU·ll1
BHTU-112 BHTU·312
Microscope stand BHTU·F 111
with quintuple revolving nosepiece
8inocular tube BH2·B130 1 1 0
Observation tubes Trinocular tube BH2·TR30 0 0 1
Square mechanical stage with right-hand low BH2.SVR2 1 1 1
drive coaxial controls and specimen holder
Swing-out condenser BH2·SC 111
Halogen lamp holder LS·20H 111
Halogen bulbs
6V20WHAL 22 2
DAch.
4X, DAch. lOX, DAch, 40X,
10 0
DAch. lOOX (oil) each
Objectives D
Plan 4X, 0Plan lOX, D Plan 40X 01 1
D Plan lOOX (oil) each each
Eyepieces, paired
WKlOX 111
Photo eyepiece NFK3.3X 001
Filter
KB·4 111
Immersion oil, bottled 111
Vinyl dust cover 111
2

II. NOMENCLATURE
The Model BHTU consists of various components and interchangeable accessories as shown in
the
photo below. Awide variety of combinations, standard or optional, is available according
to your requirements.
Eyepiece
Objective
Stage
Condenser
-
3
Observation tube
Micoscope stand
For biological use
Halogen lamp
holder
--
--
LP (Light IntensityPresetting) switch

III. ASSEMBLY
This picture illustrates the sequential procedure of assembly. The numbers Indicate the
order of assembly of various components. Remove dust caps before mounting components.
Take care to keep all glass surfaces clean, and avoid :;cratching the glass surface.
NOTE: For numbers ~ and f) please refer to explanations in detail on the next page.
e®
Condenser
(The N.A. scale
engraved on the con-
denser shou ld face the
microscope front.)
-.. To AC
®outlet
Halogen bulb
Q
CD,
~ Halogen lamp
9J holder
Condenser clamping screw
Observation tube
Observation tube clamping screw
Circular dovetail mount
Eyepjece
0)
.~
®
.g Objective
Specimen
holder
Clamping
screws
~tage L' _I .......;~;..-
Stage mounting dovetail
LP (Light Intensity Presetting) switch
4

• Explanations in detaiI
9 Mounti ng the stage
11 Loosen the stage clamping screw CD by
rotating counterclockwise. (Fig. 1)
2) Insert the stage into the mounting dove-
tail of the microscope stand slowly and
lock
with clamping screw.
fJ Mounting the observation tube
1) Loosen the clamping knob CD fully.
(Fig.21 Pull spring-loaded clamping knob
CD. This will cause the locating pin <V to·
withdraw. If the pin does not, loosen the
screw further until the pin withdraws.
2) With clamping knob CD pulled out. insert
the circular dovetail of the observation
tube into the ring dovetai I.
3) Tighten the clamping knob.
5
Fig.
Fig. 2

Photo tube
Condenser height
adjustment knob
Pre- focusi ng lever
Line
cord
-
7
Diopter adjustment
ring
X-axis low drive
control knob
Xexcursion range:
76 mm.
Y-axis low drive
control knob
Yexcursion range:
50mm.
Field iris diaphragm ring
Arrow mark (0 ....... 0indicates
increase in diaphragm diameter.

II Summary of Putting the Microscope into Operation II
MODEL BHTU
A. Match the voltage selector switch to local mains voltage (page 9).
B. Switch on the light source (page 9).
C. Place aspecimen slide on the mechanical stage.
O. Coarse focus with the lOX objective.
E. Make interpupillary distance and diopter adjustments (page 12).
F. Adjust the condenser position (page 13).
G. Swing in the desired objective.
H. Adjust light intensity.
I. Fine focus.
J. Adjust aperture iris diaphragm and field j'ris diaphragm ~page 13).
I Adjustment of Illumination System for Various Objective Powers II
Condenser
Objective Achromatic-
aplanatic Abbe Swing·out
Low power
magnification condenser
condenser condenser condenser
BH2-AAC BH2-CD BH2-SC BH2-UL-C
lX
2X
Swi ng out top
Compatible
4X
lens
lOX
20X
Compatible
40X Compatible Swing in
top
lens
BOX
- - - - - - - - - - .-
lOOX •
*N.A. is somewhat low, but still compatible with a100X objective.
(Take acopy of this page
and put it on the wall near the microscope for use as areminder of
microscopic procedure.)
OLYMP'US®
8

v. OPERATION
A. Switching on the Light Source
1) Ascertain that the voltage selector SWitch CD
is set to conform WIth the local mains voltage.
(Fig. 3)
If the switch is not correctly set. adjust it by
means of the Allen wrench provided, or a
screw-driver.
2) Place the sliding voltage control lever on the
nght side of the microscope base to a lXlSition
dosest to you (low voltage position). Switch
on the light source (ma;n SWitch (2)1. (FIQ. 3)
,.
Fig. 3
IVoltage Adjustment and Ugh! IntensityI
As you push the control lever (j) in the direction
of the ARROW. the voltage increases. and the
LED readout (2) \¥ill display the lamp voltage.
(Fig. 4)
IlP (light Intensity Presening) Switch
Apart from the light intensity set by voltage
adjustment control lever, the
LP switch can set
the light intensity as required.
Presetting the light intensity (Fig. 5)
1) Switch ON the LP switch CD.
2) Insert aslotted screwdriver into me LP adjust~
ment screw (1). Turning it clockwise will in-
crease the light intensity. Turn it until required
intensity is obtained.
*The light intensity will not change even
if the voltage adjustment lever @ with
the LP switch ON. To change the light
intensity by using the voltage adjustment
lever, switch the
LP switch OFF. To recall
the
light intensity preset, push the LP
switch to ON.
AHint on Convenient Application of the LP
Switch
For instance, when you want to use two kinds of
objective lens ahernatley. set one objective by using
the vohage adjustment
[ever. then set another
objective lens by using the LP switch. This allows
the ffght intensity 10 recallhe intensity initially preset
by switching the LP swilch ON and OFF.
This also allows the light intensity setting for
photomicrography.
9
Fig. 4
Fig. 5