Olympus BHTU User manual

MODELBHTU
BIOLOGICAL
MICROSCOPE
scanned
on
Nov.
6,
2009
by
J.G.McHone
for
personal
use
only,
not
for
sale
,.----
WARNING
------,
This
instruction
manual
is
for
use
of
your
instrument.
Before
putting
it
into
operation,
we
recommend
you
read this manual
carefully
in order to familiarize your-
self
fUlly
with
its
use
so
that
you
may
obtain
opti-
mum
performance.
OLYMPUS'"
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AX5?51
.

This
instruction
manual
has
been
written
for
the
use
of
the
Olympus
Biological Microscope
Model
BHTU.
It
is
recommended that
you
read the manual
carefully
in order
to
familiarize
yourself
fully
with
the
use
of
the microscope,
so
that
you
will
obtain
optimum
performance.
IMPORTANT
Observe the
following
points:
•Operation
1.
Always
handle the microscope
with
the care
it
deserves, and avoid
abrupt
motions.
2.
Avoid
the
use
and maintenance
of
the microscope in
direct
sunlight, high temperature and
humidity,
dust and
vibration.
3.
Only
use
the tension adjustment ring
for
altering the tension
of
the coarse adjustment
knobs. (Do
not
twist
the
two
coarse
adjustment
knobs in opposite
directions
simultaneous-
Iy,
as
this
will
cause
damage.)
4.
Make sure
that
the voltage selector
switch
on
the
bottom
base
of
the microscope stand
is
set
to
conform
with
the local mains voltage.
5.
Ground
the microscope in
case
there
is
no ground terminal in
your
mains
line
.
•Maintenance
,.
Lenses must always
be
kept clean.
Carefully
wipe
off
oil
or
fingerprints
deposited
on
the
lens surfaces
with
gauze moistened
with
asmall
amount
of
xylene, alcohol or ether.
2.
Do
not
use
organic solutions
to
wipe the surfaces
of
various
components.
Plastic parts,
especially, should
be
cleaned
with
neutral detergent.
3.
Never disassemble the microscope
for
repair.
Only
authorized
Olympus
service personnel
should make repairs.
4.
The microscope should
be
covered
with
the
vinyl
dust
cover
provided
and stored in aplace
free
from
humidity
and fungi.

I.
II.
III.
STANDARD CONFIGURATIONS
NOMENCLATURE
ASSEMBLy............
.....
CONTENTS
2
3
4
IV.
IDENTIFICATION AND FUNCTION
OF
VARIOUS COMPONENTS
V.
VI.
VII.
OPERATION
A.
Switching ON the Light Source
IVoltage Adjustment and Light Intensity I
I
LP
(Light Intensity Presetting) Switch I
B.
Placement of aSpecimen Slide .
IWhite Cover for Condenser
ICover
Glass
I
ISpecimen Slide I
IStage I
C.
Observation Tube
1.
Interpupillary Distance Adjustment
2.
Diopter Adjustment
3.
Light Path Selector
D.
Condenser Adjustment
1.
Condenser Centration
IFiled Iris Diaphragm I
IAperture Iris Diaphragm
E.
Focusing Adjustment .
1.
Tension of Coarse Adjustment
Knobs
and
Fine Adjustment
IUse of Rubber
Cap
for
Fine
Adjustment
Knob
2.
Pre-Focusing Lever
F.
Use
of Immersion Objectives
G.
Photomicrography .
OPTICAL DATA
TROUBLESHOOTING GUIDE
6
9
9
10
13
14
15
15
17
18

I.
STANDARD
CONFIGURATIONS
"",
Model
Component BHTU·ll1
BHTU-112
BHTU·312
Microscope stand BHTU·F 111
with
quintuple revolving nosepiece
8inocular tube BH2·B130 1 1 0
Observation tubes Trinocular tube BH2·TR30 0 0 1
Square mechanical
stage
with
right-hand low BH2.SVR2 1 1 1
drive coaxial controls and specimen holder
Swing-out condenser BH2·SC 111
Halogen lamp holder LS·20H 111
Halogen bulbs
6V20WHAL
22 2
DAch.
4X,
DAch.
lOX,
DAch,
40X,
10 0
DAch. lOOX (oil) each
Objectives D
Plan
4X,
0Plan
lOX,
D
Plan
40X 01 1
D
Plan
lOOX
(oil) each each
Eyepieces, paired
WKlOX
111
Photo eyepiece NFK3.3X 001
Filter
KB·4
111
Immersion oil, bottled 111
Vinyl
dust cover 111
2

II.
NOMENCLATURE
The Model
BHTU
consists
of
various components and interchangeable accessories
as
shown in
the
photo
below. Awide variety
of
combinations,
standard
or
optional,
is
available according
to
your
requirements.
Eyepiece
Objective
Stage
Condenser
-
3
Observation tube
Micoscope stand
For
biological use
Halogen lamp
holder
--
--
LP
(Light IntensityPresetting) switch

III.
ASSEMBLY
This picture illustrates the sequential procedure
of
assembly. The numbers Indicate the
order
of
assembly
of
various
components.
Remove
dust
caps
before
mounting
components.
Take care to keep all glass surfaces clean, and avoid :;cratching the glass surface.
NOTE:
For
numbers
~
and
f)
please refer
to
explanations
in detail on the
next
page.
e®
Condenser
(The
N.A.
scale
engraved
on
the
con-
denser
shou
ld
face
the
microscope
front.)
-..
To AC
®outlet
Halogen bulb
Q
CD,
~
Halogen lamp
9J holder
Condenser clamping screw
Observation tube
Observation tube clamping screw
Circular
dovetail
mount
Eyepjece
0)
.~
®
.g
Objective
Specimen
holder
Clamping
screws
~tage
L'
_I
.......;~;..-
Stage
mounting
dovetail
LP
(Light Intensity Presetting) switch
4

•
Explanations
in detaiI
9
Mounti
ng
the
stage
11
Loosen the
stage
clamping screw
CD
by
rotating counterclockwise. (Fig. 1)
2) Insert the
stage
into the mounting dove-
tail
of
the microscope stand slowly and
lock
with
clamping screw.
fJ
Mounting the observation tube
1)
Loosen the clamping
knob
CD
fully.
(Fig.21 Pull spring-loaded clamping knob
CD.
This
will
cause
the locating pin
<V
to·
withdraw.
If
the pin does
not,
loosen the
screw
further until the pin withdraws.
2)
With
clamping knob
CD
pulled
out.
insert
the circular dovetail
of
the observation
tube into the
ring
dovetai
I.
3)
Tighten the clamping knob.
5
Fig.
Fig. 2

Photo
tube
Condenser height
adjustment
knob
Pre-
focusi
ng
lever
Line
cord
-
7
Diopter
adjustment
ring
X-axis
low
drive
control knob
Xexcursion range:
76 mm.
Y-axis
low drive
control knob
Yexcursion
range:
50mm.
Field iris diaphragm ring
Arrow
mark
(0
.......
0indicates
increase
in
diaphragm diameter.

II
Summary
of
Putting
the Microscope
into
Operation
II
MODEL
BHTU
A.
Match
the
voltage
selector
switch
to
local mains voltage (page 9).
B.
Switch on the
light
source
(page
9).
C.
Place aspecimen slide on
the
mechanical stage.
O.
Coarse focus
with
the
lOX objective.
E.
Make
interpupillary
distance
and
diopter
adjustments
(page
12).
F.
Adjust the condenser position
(page
13).
G.
Swing
in
the
desired objective.
H.
Adjust
light
intensity.
I.
Fine focus.
J.
Adjust
aperture iris diaphragm and
field
j'ris
diaphragm
~page
13).
I
Adjustment
of
Illumination
System
for
Various Objective Powers
II
Condenser
Objective Achromatic-
aplanatic Abbe Swing·out
Low
power
magnification condenser
condenser
condenser condenser
BH2-AAC BH2-CD BH2-SC BH2-UL-C
lX
2X
Swi
ng
out
top
Compatible
4X
lens
lOX
20X
Compatible
40X
Compatible Swing in
top
lens
BOX
- - - - - - - - - - .-
lOOX •
*N.A.
is
somewhat low,
but
still compatible
with
a100X objective.
(Take acopy of this page
and
put it
on
the wall near the microscope for use as areminder of
microscopic procedure.)
OLYMP'US®
8

v.
OPERATION
A.
Switching
on
the Light Source
1)
Ascertain that the voltage selector SWitch
CD
is
set
to
conform
WIth
the
local
mains
voltage.
(Fig.
3)
If the switch is not correctly set. adjust
it
by
means
of
the
Allen wrench
provided,
or
a
screw-driver.
2)
Place
the sliding voltage control lever
on
the
nght
side
of
the
microscope
base
to a
lXlSition
dosest
to you
(low
voltage
position). Switch
on
the
light
source
(ma;n
SWitch
(2)1.
(FIQ.
3)
,.
Fig. 3
IVoltage Adjustment and
Ugh!
IntensityI
As
you
push the
control
lever
(j)
in
the direction
of
the
ARROW.
the
voltage
increases.
and
the
LED readout
(2)
\¥ill display the lamp voltage.
(Fig. 4)
IlP
(light
Intensity Presening) Switch
Apart from the light intensity set
by
voltage
adjustment control lever, the
LP
switch
can
set
the light intensity as required.
Presetting the
light
intensity (Fig.
5)
1) Switch ON the
LP
switch
CD.
2)
Insert aslotted screwdriver into
me
LP
adjust~
ment screw
(1).
Turning it clockwise will
in-
crease the light intensity. Turn it until required
intensity is obtained.
*The
light
intensity
will
not
change even
if the voltage adjustment lever @
with
the
LP
switch ON. To change the
light
intensity
by
using the voltage adjustment
lever, switch the
LP
switch
OFF.
To recall
the
light
intensity preset, push the
LP
switch to ON.
AHint on Convenient Application
of
the
LP
Switch
For
instance, when you want to use
two
kinds
of
objective lens
ahernatley.
set
one
objective
by
using
the vohage adjustment
[ever.
then set another
objective lens by using the
LP
switch. This allows
the
ffght
intensity
10
recallhe intensity
initially
preset
by
switching the
LP
swilch ON and
OFF.
This also allows the light intensity setting for
photomicrography.
9
Fig.
4
Fig.
5

.
..
.~~
~~
....
-
~
/~-"'"'"
Fig. 6
B.
Placement
of
a
Specimen
Slide
11
Rotate the coarse adjustment knobs
CD
in
the
direction of the ARROW to rack down the stage
so
that aspecimen slide can
be
placed
on
the
stage.
(Fig.
6)
NOTE: The rotation of the coarse and fine
adjustment knobs
in
the direction of the
ARROW will rack
down
the stage.
2)
Opening the spring-loaded finger of the speci-
men holder with one hand, place aspecimen
slide Inside the holder
with
the other hand.
When the slide comes
in
contact
with
the back
of the specimen holder, slowly return the spring-
loaded finger.
WARNING: If the spring-loaded finger
IS
re-
turned
t.oo
quickly, it may cause
damage
to
the specimen slide.
3)
For
use
of
the specimen holder B2-HL2
Aspecimen slide
can
be easily slipped into the
specimen holder by single hand operation. (Fig.,
71
Fig.
7
White
Cover
for
Condenser
The white cover for condenser
CD
should
be
covered over the condenser.
(Fig.
8)
•When setting stain specimens under the
specimen holder, the white cover for condenser
comes into field
of
view
as
abackground. This
allows easy distinction
what
you are observing
between its background.
10

ICover
Glass
I
•An
Olympus
objective engraved "16010.17" requires acover
glass
of
0.17mm
thickness.
If
the
numerical aperture
of
the objective
is
0.7
or
higher (except immersion objectives)
and
no correction collar
is
provided, the resolving power deteriorates greatly,
if
cover
glass
thickness deviates
from
the above listed value.
NOTE:
In some countries a0.17
mm
thick
cover
glass
corresponds
to
a#1
Y,
cover
glass.
• A cover
glass
(0.4
mm
thick)
for
blood
counting, etc.
can
be
used
with
Olympus
objectives
except 0Plan
40X.
SPlan
Apo
40X
and SPlan lOOX.
ISpecimen Slide I
•Specimen slides 0.8 mm
to
1.5 mm
thick
are
recommended for
Olympus
objectives.
•Specimen slides 0.8
mm
to
1.2
mm
thick
are
recommended for, the
dark
field condenser
and the
dj
fferential interferencecontrast
con~
denser.
3) Bring the
portion
of
the specimen
to
be
observed
into
the
light path
by
means
of
the
low
drive
stage
control
knobs.
(Fig.
9)
*Tighten the
stage
clamping screw
<D
.
I
Stage
I
•
The
specimen holder
can
accommodate
two
standard specimen slides simultaneously.
•The specimen holder
is
removalbe
to
obtain
alarge unobstructed stage surface to hold
specimens up
to
55 mm x
85
mm.
•
To
rotate the
stage
loosen the
$tage
clamping
screw
CD
and holding this screw, rotate the
stage
into
the desired direciton.
(Fig.
10)
@Stage clips
for
use
with
immersion objectives.
(Fig.
111
Apair
of
stage clips
is
optionally
available
to hold the specimen
on
the stage, elimina-
ing specimen drag
or
buckling
due
to
immer-
sion
oil
between slide and stage surface. The
cI
ips
can
be
inserted
into
the holes
CD
provided on the specimen holder.
11
Fig.10
Fig.
11

c.
Observation Tube
1.
Interpupillary
Distance
Adjustment
1}
Click
the
lOX
objective
into
position.
2)
Looking
through the eyepieces
with
both
eyes, adjust the
interpupillary
distance
of
the
binocular
tube
by
adjusting the
knurled dovetail slides
CD
of
the
right
and
left
eyepiece tubes
with
b9th
hands
until
perfect
binocular
vision
is
obtained. {Fig.
121
-
Fig. 12
2.
Diopter
Adjustment
1)
Looking
at the image
through
the
right
eyepiece
with
your
right
eye, focus on
the specimen
with
the
fine
adjustment
knobs.
21
Next,
looking
at
the image
through
the
left
eyepiece
with
your
left
eye, rotate
the
diopter
adjustment
ring
CD
to
focus
on the specimen
without
using the
coarSE
and fine
adjustment
knobs..
(Fig.
131
3.
Light
Path Selection
1)
The
trinocular
tube
is
provided
with
a
light
path selector lever
CD
to
direct
the
light
to
the observation
tube
and/or
to
the
photo
tube in 3positions. (Fig.
141
Indicator
Plate
~J
./11
tii
-
,,~
&,~
:t
\'i~
,
1lIII\,'
~
_f'~"
~
.~
-.:.$
[1
'
Fig. 14
CD
r
Lever Position
Pushed
in
all the
way
Pulled out:
halfway
Pulled
out
all
the
way
(VI
(C.
11.1
(C)
Amount
100%
into
binocular
tube 20%
into
binocular
tube 100%
into
photo
tube
of
light
80%
into
photo
tube
CD
Observation
CD
Observation
of
exces- Photomicrography
of
Applica·
sively
bri~lht
specimens
dark
specimens
CV
Dark
specimens
~
Photomicrography
(fo·
tion
cusing
through
the bi-
nocular
tube)
An
indicator
plate
is
provided at the knob
port
to
summarize the
usage
of
the
above table;
it
can
be
consulted before operating the
knob
V:
Viewer
(white
letters)
CV:
Camera &viewer (yellow-green letters)
C: Camera (red letters)
The
colors
of
the letters correspond wi
th
the
color
bands
on
the knob shaft.
12

D.
Condenser
Adjustment
®
Fig. 14
Fig.
15
1. Condenser Centration
1)
Stop
down
the
field
iris
diaphragm
with
knurled
ring
CD
by
rotating
in
the
direc-
tion
of
the
arrow.
(Fig. 14)
2) Use
the
condenser
height
adjustment
knob
®
to
move
the
condenser
up
and
down
until
an image
of
the
field
dia-
phragm can be
seen
clearly
in
the
eye-
pieces.
The
rotation
of
the
knob
in
the
direction
of
the
arrow
lowers
the
con-
denser.
Field iris
diaphragm
image
Field
of
view
3)
Bring
the
field
iris
diaphragm
image
into
the
center
of
the
field
of
view
with
the
two
con-
denser
centering
knobs
®.
(Fig. 14)
4)
Widen
the
diameter
of
the
iris diaphragm progressively.
If
the
polygonal image
of
the
iris
diaphragm
becomes
inscribed
in
the
field
it
means
that
the
field
diaphragm
is centered.
(Fig.
15)
Field
Iris
Diaphragm
I
•
The
field
iris
diaphragm
controls
the
diameter
of
the
ray
bundle
impining
on
the
speci-
men
surface
and
therefore,
by
stopping
down
the
field
diaphragm
until
it
is
slightly
larger than
the
field
of
view,
it
can reduce stray light, which
in
turn increases image defi-
nition
and contrast.
70-80%
20
-30","
Fig.
16
Opening
of
the
aperture diaphragm
Objective
exit
pupil
Aperture
Iris Diaphragm I
•In order
to
achieve opTimum objective performance,
the
opening
of
the aperture iris
diaphragm should be matched
to
the
numerical aperture
of
the
objective in
use.
It
is
often
preferable, however, to stop
down
the aperture diaphragm slightly more than in-
dicated
by
the
objective
N.A.
This
will
result
in
better
image contrast, increased
depth
of
focus
and
a
flatter
field.
•
After
completing focus
adjustment,
re-
move one
of
the
eyepieces
from
the
ob-
servation
tube
and
look
into
the
empty
eyepiece tube. As
you
stop
down
the
aperture iris diaphragm, the image
of
the
iris diaphragm can
be
seen
in
the object-
ive
pupil.
Adjust
the
opening
of
the
diaphragm
to
match
the
N.A.
of
the
ob-
jective
in
use.
If
the
specimen
is
low
in
contrast,
it
is
recommended
to
stop
down
to
70%
-
80%
of
the
objective
N.A.
(Fig.
161
13

EFocus Adjustment
Fig.
18
f
Adjustment Knobs
and
Although
the
tension
of
the
coarse
adjust-
ment knobs
has
been
already adjusted for
optimum performance by the manufacturer.
it
is
possible
to personally adjust the tension
of
the
coarse
adjustment for either heavy or
light movement depending
on
the operator's
preference by rotating
the
tension adjust-
ment ring
<D.
(Fig.
181
The
ring
can
be
rotated by inserting ascrewdriver into
one
of the holes
on
the periphery
of
the
ring.
The clockwise rotation
(in
the direction of the
ARROW)
tightens the coarse adjustment
knobs.
Do
not loosen the ring too much, because the
stage
may drop or the fine adjustment
knobs may slip.
CD
1.
Tension
of
Coarse
Fine Adjustment.
NOTE:
Do not rotate the right
and
left
coarse
adjustment knobs
in
the
opposite directions
simultaneously. If the
stage
drops
and
the
specimen
goes
out
of
focus. the tension
adjustment
ring
is
too
loose.
Tighten the
ring.
Use
of Rubber
Cap
for Fine Adjustment Knob,
Attaching this
cap
over
the
fine adjustment
knob
increases
the sensitivity
of
the fine focus-
ing
motion. (The rubber
cap
is
optionally
available.)
2.
Pre-Focusing
Lever
This
lever
ill
is
provided to prevent
possible
contact between
specimen
and
objective
as
well
as
to
simplify
coarse
focusing.
(Fig.
191
The
lever
is
locked after
coarse
focus
has
been
accomplished. This prevents further
upward travel
of
the
stage
by
means
of
the
coarse
adjustment knobs,
and
automatically
provides alimiting stop if the
stage
is
lowered
and
then
raised
again.
The pre-focusing
lever
does
not restrict fine
focusing.
Fig.
19
14

F.
Use
of
Immersion Objectives
1)
Focus the specimen
with
a
low
power
objective.
2)
Put a
drop
of
immersion
oil
on the specimen slide and the
front
lens
of
the immersion
objective.
3)
Rotate the revolving nosepiece
to
bring the immersion objective
into
the light path, and
focus
with
the fine adjustment knobs.
NOTE:
ill
For
immersion condensers such
as
an
achromatic-aplanatic condenser
or
Abbe
condenser, remove the specimen
from
the
mechanical stage and place a
drop
of
immersion
oil
on
the
front
lens
of
the condenser. Then, place
the
specimen
on
the
stage
and slowly raise
the
condenser
until
firm
contact
with
the underside
of
the specimen slide
is
made.
®Care should be taken
to
prevent
oil
bubbles
from
forming
in
the
oil
film
between
condenser and specimen slide.
If
any bubbles appear, re-apply immersion
oil,
for
these bubbles greatly deteriorate
the
lens performance.
®
After
use.
carefully wipe
off
the immersion
oil
deposited
on
the lens surfaces
with
gauze moistened
with
xylene. Never leave oil on the lens surfaces
after
use
as
oil
remnants
will
seriously impair the perforrrance
of
the
lens
system.
,
G. Photomicrography
The
Olympus
Photomicrographic Equipment Model
PM-lOAO
is
uniquely
qualified
to
be
used
with
the
BHTU
microscope
for
routine
and advanced
photomicrography.
Aseparate,
detailed instruction manual
is
available for the
PM-lOAD
camera system.
For
quick
reference, however,
you
may want
to
refer
to
the
following
pointers when using
the
PM-lOAD.
1.
Photographic Eyepiece
Use
NFK
photo
eyepieces
for
photomicro-
graphy.
Insert
the
eyepiece
CD
into
the eyepiece tube
of
the
photo
tube.
(Fig.
20)
Fig. 20
2.
Mounting
the Photographic
Unit
Slip the
body
of
the photographic
unit
over the
photo
tube.
Align
the
dots on
photo
tube
and
the PM-
lOAD
body
and clamp the camera
unit
to
the photo tube.
(Fig.
211
3.
Setting the Light Path Selector
Refer
to
section C.3. on
page
12.
15

Fig.
22
4.
Focusing Procedure
Use
the field
of
view eyepieces
for
focusing on the
film
plane. Each field
of
view eyepiece
has
afocusing
front
lens and a
relide
with
4frames, each frame indicating
the
area covered
by aspecific power NFK
photo
eyepeice.
(Fig.
22)
The
number
at
each
frame indicates the
magnification
of
the
photo
eyepiece. The
image in
the
field
of
view eyepiece and
the
image
on
the
film
plane are
in
focus at the
same
time. Several
type
field
of
view eye-
pieces
are
available, according
to
the
film
size employed.
Field
of
view eyepiece 35WHK10X PWHK10X
4X5WHK10X
MHWHK10X
35mm
Back
3%"
x
4%"
4"
x
5"
16mm
Bolex
Attachment
camera Polaroid Sheet Film or camera
Back Polaroid
Film
120 Roll Film
Holder Holder
1)
Select the field
of
view eyepiece matching
the
camera
bSlck
in
use
and insert
it
into
the
right eyepiece tube
of
the
trinocular
tube, aligning locating groove and locating pin.
2) While
looking
through the
field
of
view eyepiece, rotate the eyepiece
front
lens
in
screw
mount
to
focus on the
double
cross lines in
the
field.
For
sharp focusing
with
objectives
4X
or
lower,
the
focusing magnifier
FT
is
recommended on account
of
their
considerable
depth
of
focus.
3)
Bring
the
specimen detail
to
be
photographed
within
the frame corresponding
to
the
power
of
the NFKeyepiece
in
use
and focus on
the
specimen
with
the microscope fine
adjustment knobs. Make sure the
light
path selector
knob
on
the
observation tube
is
either on the white
(V)
or
yellowijreen
(eVI
band.
4)
It
is
recommended to
tighten
the tension adjustment ring considerably
to
prevent
the
stage
from
dropping
during
long exposures.
16

VI. OPTICAL
DATA
Objective
Type
o
Achromat
oPlan
Ach.
Magnifi-
4X
lOX
40X
l00X'
4X lOX 40X
100X'
cation
N.A.
0.10
0.25 0.65
1.30
0.10
0.25 0.65
1.25
W.O.
(mm)
18.2
7.2
0.6
0.20
7.03
7.4 0.27
0.17
Focal length
30.03
16.9
4.58
1.91
34.23
17.5
4.67
1.75
lmml
Eyepiece Resolving
3.36
1.34
0.52
0.26
3.36
1.34 0.52
0.27
power (jJ)
WK10X
Total mag. 40X 100X
400X
1000X
40X
100X
400X
lQOOX
(Field
Focat
171.6
27.45
3.0
0.7
171.6
27.45
3.0
0.7
number depth
{jJJ
201 Field
of
5 2
0.5
0.2
520.5
0.2
view
(mm)
ll-Immersion
objectives
The resolving power and focal depth are obtained
witt'
fully
opened aperture diaphragm.
Technical
terms:
•Working distance:
The
distance
from
the
cover
glass
to
the
nearest
point
of
the
objective.
•Numerical aperture: The N.A. represents aperformance number which
can
be
com-
pared to the relative aperture (f-number) of acamera lens.
Th~
N.A.
values can
be
used
for
directly
comparing
the
resolving
powers
of
all types
of
objectives. The larger the
NA.
the higher
resolving
power.
•
Resolving
power: The ability
of
a
lens
to register small details.
The
resolving
power
of a
lens
is
measured
by
its ability to
separate
two points.
•Focal depth: The distance between the upper
and
lower limitsof
sharpness
in
the
image
formed by
an
optical system.
As
youstop down the
aperture
iris
diaphragm, the focal depth
becomes
larger. The
larger
the N.A.
of
an
objective the shallower
the
focal depth.
•Field number: Anumber that represents the diameter
in
mm of
the
image
of
the field diaphragm that is formed
by
the lens in
front
of
it.
•Field
of
view diameter: The actual
size
of the field of view
in
mm
on
the object surface.
17

VII.
TROUBLESHOOTING
GUIDE
If
you
are unable
to
obtain
full
performance
from
your
microscope,
please
consult
with
the
table
below
as
pointers
for
troubleshooting.
Phenomenon Cause
Remedy
1.
Optical
System
a)
With
illuminator
switched
Field iris diaphragm
IS
not
Open
diaphragm
to
proper
on,
the field
of
view
is
opened
sufficiently.
diameter.
dark. Condenser
is
lowered
too
Adjust
condenser height.
much.
Light
path selector lever
is
Push in lever
up
to
CV
or
V
pulled
out
to
Cposition.
position.
b) Field
of
view
is
cut
off
or
Light
path selector lever
is
Click
it
into
proper
position
illuminated
irregularly.
stopped
midway. according
to
your
purpose.
Nosepiece
is
not
clicked
into
Slightly
rotate
nosepiece until
place.
it
cl
icks
into
place.
The
power
of
objective used Choose acondenser
to
meet
exceeds
the
illu'mination
ca-
your
purpose.
pacity
of
condenser.
Condenser is
not
centered. Center condenser.
Field
irisdjaphragm
is
stopped Open diaphragm to
proper
down
excessively. diameter.
c)
Dust
or
dirt
is
visible
In
Dust, etc. on
light
exit
lens Remove
dust,
etc.
the
field
of
view.
Dust
on condenser
top
lens Clean
front
tenses.
Dirty
specimen
Dust
on eyepiece
d) Excessive image contrast Condenser
is
lowered
too
Adjust
condenser height.
much.
Aperture
iris diaphragm
is
Open diaphragm to
proper
stopped
down
excessively. diameter.
18

Phenomenon
Cause
Remedy 1
el Resolution problems:
LB
objectives
other
than
Use
Olympus
LB
series objec-
series are used. tives.
•I
mage
is
not
sharp. objectiv8
not
correctly
Click
nosepiece
into
place.
I
IS
•
Insufficient
contrast. positioned in
the
light path.
•Image details lack defi-
nition.
Objective correction collar
is
Rotate
correction
collar, keep-
not
adjusted
correctly.
ing specimen in fine focus
until
optimum
resolution
is
obtained.
Dust on objective
front
lens Clean
front
lens.
Immersion objective
is
not
Use
immersion
oil.
used
with
immersion
oil.
Bubbles in immersion oil Remove bubbles (and reap-
ply
oil).
Immersion oil designated
by
Use
Olympus
immersion oil.
Olympus
is
not
w~ed.
Dirty
specimens Clean.
Dust on condenser
lens
fl
Field
of
view
is
partially
Objective
is
not
correctly
Slightly
rotate nosepiece
until
out
of
focus,
or
image
is
positioned in the
light
path.
it
clicks in place.
partly
out
of
focus. Specimen
is
not
correctly
Place specimen slide
correctly
positioned on stage. on stage, and place
stage
clips
open
it.
gl Specimen image
is
partial- Objective
is
not
correctly
Slightly
rotate nosepiece
until
Iy
out
of
focus. positioned in the
light
path.
it
clicks
into
place.
Condenser
is
not
centered. Center condenser.
h) Field
of
view becomes Condenser
is
not
correctly
Center condenser.
only
slightly
brighter
by
centered.
increasing voltage. Condenser
is
lowered
too
Adjust
condenser height.
much.
2.
Electric System
al
Illuminator
is
too
bright
Line voltage selector switch Match selector switch
to
(or
too
dark)
even
when
is
not
matched
with
local mains voltage.
adjusting
control
lever. mains voltage.
b)
Voltage
for
illuminator
cannot
be
raised.
cl Lamp
goes
off
and on. Bulb
filament
is
likely
to
Replace bulb.
burn
out.
Loose electric connections. Check all connections.
d)
Bulb burns
out
frequently.
Line
voltage selector switch
is
Match
selector switch
to
not
matched
with
local mains mains voltage.
voltage.
Bulb
is
not
standard one.
Use
standard bulb.
19
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