Olympus bx40 User manual

INSTRUCTIONS
BX40
SYSTEM MICROSCOPE
This instruction manual is for the Olympus System Microscope Model BX40. To ensure the safety,
obtain Optimum Performance, and to familiarize yourself fully with the use of this microscope,
we recommend that you study this manual thoroughly before operating the microscope. Retain
this instruction manual in an easily accessible place near the work desk for future reference.
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AX7125

This unit employs a UIS (universal infinity System) Optical design, and should be used only
with UIS eyepieces, objectives, and condensers. Less than Optimum Performance may
result if inappropriate accessories are used.
1. Install the microscope on a sturdy, level table or bench.
The air vents on the underside of the base should never be blocked such
as by placing the microscope on a flexible surface, as this could result in
overheating and Cause a fire.
2. To prevent obstruction of the natura1 convection of this microscope, make
sure to leave at least 10 cm of free space between Walls, or other objects,
and the left, right and rear sides of the microscope and the lamp socket
when installing this microscope.
3. To avoid potential shock hazards and burns, first set the main switch 0 to
“0” (OFF) and then disconnect the power cord from the wall outlet before
replacing the bulb. Whenever you replace the bulb during use or right after
use, allow the lamp socket @ and bulb to cool before touching.
Designated bulb 6V 30WHAL-L (PHILIPS 5761)
4. Make Sure that the voltage selector switch @ located on the rear side of
the microscope frame is set to conform with the local mains voltage. (When
shipped from the factory, the switch is set to 220-240V.)
5. Always use the power cord provided by Olympus. If no power cord is
provided, please select the proper power cord by referring to the section
“PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of
this instruction manual. If the proper power cord is not used, product safety
and Performance cannot be guaranteed.
6. Always ensure that the ground terminal of the microscope and that of the
wall outlet are properly connected. If the equipment is not grounded,
Olympus tan not longer Warrant the electrical safety and Performance of
the equipment.
7. Never insert metal objects, etc. into the air vents of the microscope frame
as this could result in electrical shock and personal injury.

) Safety Symbols )
The following Symbols are found on the microscope. Study the meaning of the Symbols, and always use the equipment
in the safest possible manner.
Indicates that the surface becomes hot, and should not be touched with bare hands.
Before use, carefully read the instruction manual. Improper use could result in personal
injury to the user and/or darnage to the equipment.
Indicates that the main switch is ON.
Indicates that the main switch is OFF.
Warning engravings/stickers are placed at Parts where special precaution is required when handling and using the
microscope. Always heed the warnings.
Warning engraving Position Lamp socket [Warning against high temperaturel
Warning sticker Position Microscope frame rear Panel [Warning against high temperaturel
Should warning stickers become soiled, peel off, etc. contact Olympus for replacements.
a
GetZing Ready
1. A microscope is a precision instrument. Handle it with care and avoid
subjecting it to sudden or severe impact.
2. DO not use the microscope where it is subjected to direct sunlight, high
temperature and humidity, dust or vibrations. For operating conditions,
refer to Section 6, “SPECIFICATIONS” on page 21.
3. When moving the microscope, carefully carry it with both hands by grasping
the arm as shown in the figure on the left. (Weight: approximately 12.5 kg
(28 Ib.)
J, Darnage to the microscope may occur if you grasp it by the Stage,
coarse adjustment knob, or binocular section of the Observation
tu be, etc.
4. The 5x40 tan be used with up to two intermediate attachments (e.g., a
U-CA magnification changer and/or U-EPA eyepoint adjuster). For
restrictions when using additional intermediate attachments, make sure
to read the instruction manual provided with the respective intermediate
attachments.

a., Maintenance and Storage
1. Clean all glass components by wiping gently with gauze. To remove fingerprints or oil smudges, wipe with gauze
slightly moistened with a mixture of ether (70%) and alcohol (30%).
A Since solvents such as ether and alcohol are highly flammable, they must be handled carefully. Be sure
to keep these chemicals away from open flames or potential sources of electrical Sparks - for example,
electrical equipment that is being switched on or off. Also remember to always use these chemicals only
in a weil-ventilated room.
2. DO not attempt to use organic solvents to clean the non-Optical components of the microscope (especially plastic
Parts). To clean these, use a lint-free, soft cloth lightly moistened with a diluted neutral detergent.
3. DO not disassembly any part of the microscope as this could result in malfunctions or reduced Performance
4. When not using the microscope, keep it covered with the provided dust cover.
’Caution
If the attachment is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition,
the equipment may also be damaged. Always use the equipment as outlined in this instruction manual
The following Symbols are used to set off text in this instruction manual.
A : Indicates that failure to follow the instructions in the warning could result in bodily harm
to the user and/or darnage to equipment (including objects in the vicinity of the equipment).
* : Indicates that failure to follow the instructions could result in darnage to equipment.
0 : Indicates commentary (for ease of Operation and maintenance).

BX40
2-l Assembly Diagram ........................................................................ 2
2-2 Assembly Procedure ..................................................................... 3
a 1
5-1 Base ................................................................................................. 10
5-2 Stage ............................................................................................... 12
5-3 Observation Tube .......................................................................... 14
5-4 Condenser ....................................................................................... 16
5-5 Focusing Adjustment Knobs ........................................................ 18
5-6 Immersion Objectives ................................................................... 19
5-7 Photomicrography ......................................................................... 19
n PROPER SELECTION OF THE POWER SUPPLY CORD ........................... 26

__/______
1 Eyepiece 1
Observation Tube
Revolving Nosepiece
Lamr, Socket 1
~ 1 0

The diagram below Shows the sequence of assembly of the various modules. The numbers in&& the Order of assembly,
* When assembling the microscope, make Sure that all Parts are free of dust and die, and avoid scratching any _
Parts or touching glass sutfaces.
! k
Eyepiece Observation tube
Allen screwdriver
Halogen bulb
Microscope frame
Condenser
cord
0
2

Fig. 1
Fig. 2
Fig. 3
a
USe Onlya designated bulb 6V, SOWHAL halogen bulb (PHILIPS 5761). (
1. Holding the bulb @ with gloves or a piece of gauze, insert the bulb pins
@ fully into the pin holes 0. (Fig. 1)
* To prevent reduced bulb life or cracking, do not tauch the bulb
with bare hands. If fingerprints are accidentally left on the bulb,
wipe the bulb with a soft cloth.
2. Aligning the guide pins @ with the guide pin holes @ at the rear of the
microscope frame, and the plug @ with the socket Q), gently push the
lamp socket into place (Fig. 2)
A Bulb Replacement During Use or Right After Use
The bulb and the lamp socket and areas near these will be extremely
hot during use and right after use.
Press the main switch to “0” (OFF) and disconnect the power cord
from the Wall outlet. Then allow the old bulb and lamp socket to
cool before replacing the bulb with a new of the designated type.
1. Loosen the clamping screw @ at the front of the Stage.
2. Gently lower the Stage onto the substage dovetail @ and then tighten
the clamping screw (iJ
l
l 3
0

Fig. 4
Fig. 5
Fig. 6
Fig. 7
a
Attaching the Slide Holder
(Fig. 4)
1. Loosen the slide holder clamping screws (iJ to the extent that they
just clear the thickness of the slider holder.
* If the clamping screws are loosened too much, their threaded
portions may interfere with the Slots in the slide holder.
2. Insert the slide holder from the front until it Comes up against the stop.
At this Point, make sure the slide holder clamping screws fit into the
positioning Slots on the specimen holder.
* Ifthe slide holder is not fully inserted, it may attach in a tilted Position.
3. Tighten the slide holder clamping screws.
a
1
Mounting the Condenser
(Fig. 5)
1. Turn the coarse adjustment knob Q) to raise the Stage to its upper limit.
2. Turn the condenser height adjustment knob @ to lower the condenser
holder to its lowest Position.
3. Loosen the condenser clamping screw 0.
4. Position the condenser so that the aperture scale is located in front.
Gently slide the condenser along the dovetail.
5. Tighten the condenser clamping screw and gently raise the condenser
holder to its highest Position.
t When mounting the U-SC Swing-out condenser, align the
positioning pin at the back of the condenser with the groove in the
condenser sleeve.
* When using the U-SC Swing-out condenser or the U-UCDIUCDB
universal condenser, Swing the top lens out of the way before
inserting the condenser.
a
Mounting the Objectivec;
(Fig. 61
1. Turn the coarse adjustment knob to lower the Stage to its lowest position.
2. Mount the objectives on the revolving nosepiece in such a manner that
the magnification increases from low to higher in a clockwise direction.
a
.
Mounting the Observation Tube
(Fig. 7)
1. Using the Allen screwdriver, loosen the Observation tube clamping screw
0.
2. Insert the circular dovetail mount at the bottom of the Observation tube
into the opening on the microscope frame, placing the Observation tube
to Point the binocular eyepieces towards the observer. Clamp the
Observation tube by tightening the clamping screw.
Ir If the direction of Stage movement and the direction of image
movement differs during observations, loosen the Stage clamping
screw slightly and adjust the Stage by rotating it, while obser-ving
the image.
0 4

Fig. 8
Fig. 9
Fig. 10
1. Remove the eyepiece sleeve dust taps (iJ.
2. Insert the eyepieces @ into the eyepiece sleeves as far as they will
go. (Fig. 8)
Ir When using the binocular tube, helicoid equipped focusing
eyepieces cannot be used.
When using the trinocular Observation tube (U-TRBO) or super wide-
field trinocular Observation tube (U-SWTR)
When using a finder eyepiece or an eyepiece with micrometer adjustment,
insert this eyepiece into the right-hand eyepiece sleeve. When doing so,
make Sure that the eyepiece positioning pin @ fits into the notch @ on
the eyepiece sleeve. (Fig. 9)
QThe super-widefield trinocular Observation tube is equipped with a
positioning notch on both eyepiece sleeves. Make sure that the
positioning pin on both eyepieces fits into the respective notch.
A The power Cord is vulnerable when bent or twisted. Never subject
it to excessive forte.
A Make Sure that the main switch @ is on “0” (OFF)before connecting
the power Cord. (Fig. IO)
A Always use the power Cord provided by Olympus. If no power
cord is provided, please select the proper power cord by referring
to the section “PROPER SELECTION OF THE POWER SUPPLY
CORD” at the end of this instruction manual.
1. Connect the power Cord plug @ to the AC receptacle 0. (Fig. 10)
2. Plug the power Cord plug @ into a Wall outlet. (Fig. 11)
A Connect the power cord correctly and ensure that the ground
terminal of the power supply and that of the wall outlet are properly
connected. If the equipment is not grounded, Olympus tan no
longer Warrant the electrical safety and Performance of the
equipment.
Fig. 11
5 0

BX40
Interpupiliary distance adjustment scale
/
Diopter adjustment ring
Slide holder
Condenser aperture
iris diaphragm ring
Y-axis knob /
X-axis knob /
Pre-focusina lever
Allen screwdriver
Condenser height
adjustment knob
Voltage
indicator scale
Light intensity lever
Light intensity
preset adjustment
screw
Light intensity
preset button
Field iris diaphragm ring
Filter mount
l
6

0
0
\ Il;,e selector
7
0

l I
1. Press the main switch to ” 1” (ON) and adjust the brightness with the light
intensity adjustment lever. (At this Point, keep the light intensity preset button
OFF.) (p. IO)
2. Disengage all filters from the light path. (p. IO)
3. Turn the revolving nosepiece to engage the 1OXobjective. Make sure the revolving
m 1
nosepiece clicks into Position. z
:
2
4. Place a specimen on the Stage. (p. 12)
5. Turn the X-axis knob @ and the Y-axis knob @ to move the specimen into the
light path.
0 Using a long Stage knob is convenient for clinical inspection
[Using a trinocular Observation tubel
6. Push the Observation tube’s light path selector knob to “100% for binocular
eyepieces” (the pushed-in position). (p. 15)
7. Looking through the right eyepiece with your right eye, turn the coarse
adjustment knob to bring the specimen into focus. After obtaining approximate
focus, use the fine adjustment knob to make fine adjustments. (p. 18)
0
8

8. Looking through the left eyepiece with your left eye, turn the diopter adjustment
ring to focus the specimen. (p. 14)
9. Adjust the interpupillary distance of the eyepieces. (p. 14)
10. Adjust condenser centration and focus. (p. 16)
11. Engage the objective to be used and readjust the light intensity to the desired
level for Observation, then readjust the focus.
12. Engage your choice of filters in the light path. (pp. IO,1 1)
13. Adjust the field iris diaphragm. (p. 16)
14. Adjust the aperture iris diaphragm. (p. 17)
9 0

-
BX40
Fig. 12
Fig. 13
a
Valtage hdicator
(Fig. 12)
1. Sliding the light intensity adjustment lever @ upward increases the
voltage, making illumination brighter.
2. The numerals to the right of the voltage indicator LEDs @ indicate the
voltage.
‘Engaging the Light k&!nSity hZSE!t &utton (Fig. 131
The light intensity preset button @ makes it possible to limit the light
intensity to a preselected level regardless of the Position of the light
intensity adjustment lever. SJ
z
1. Push the light intensity preset button @ to the ON Position. (The face Cl
of the button lights when the button is ON.)
2. Using a small screwdriver, turn the preset adjustment screw @ to 2
obtain the required light intensity. Turning the screw clockwise increases 8
brightness. 5
3. Switch the light intensity preset button OFF and brightness returns to 8
the level set by the light intensity adjustment lever. L
* Moving the light intensity lever does not affect brightness while
the light intensity preset button is ON
- Using the Light Intensity Preset Button
The light intensity preset button allows you to temporarily adjust brightness to a preset level for applications such
as photomicrography, making it unnecessary to manually adjust the brightness each time you take a Photograph.
0 Before shipment from factory, the preset level is set to an intensity suggested for photomicrography.
OThe light intensity preset button is also useful when using two different objectives alternatingly, helping you to
avoid manually adjusting the brightness each time you Change magnification.
Fig. 14
Accessory Filter (Fig. 14) 1
You tan place a 45 mm diameter filter in the filter holder on the light exit
at the base of the microscope. If you need to use two or more filters at
once, use a filter cassette.
Ir When using a filter cassette, you tan additionally use a Single
filter with a thickness of less than 3 mm over the light exit glass.
* When using interference filters, such as 45LBD-IF, in the filter holder
over the light exit glass, flares may become conspicuous. lf this
occurs, slightly tilt the filter.
0
IO

Fig. 15
Fig. 16
Fig. 17
8
Using the Filter Cassette (Figs. 15-18) 1
If you need to place 3 or more filters over the light exit window, use
a filter cassette.
Loading Filters Into the Filter Cassette (Fig. 15)
0 The filter cassette accommodates filters with a diameter of 45 mm and
a thickness of 2.7 mm or less.
0 The filter cassette has two filter levers on the right side and one on the
left side.
1. Move all filter levers to the OUT Position except for the one belonging
to the slot into which the filter is to be inserted.
2. Slide lever Q to the IN Position. Make sure that it clicks securely into
place. (Fig. 15)
3. Holding the lever in the Position shown in Fig. 15, put the filter into the
cassette by inserting it in the direction indicated by the arrow.
4. Insert the other two filters in the same manner.
Mounting the Filter Cassette (Figs. 16,17)
1. Fully loosen the filter cassette clamping screw 0. (Fig. 16)
2. Holding the filter cassette above the light exit glass, align the key @
with the slot @ and snap the filter cassette into place from above.
3. Rotate the filter cassette to align its sides with the base. (Fig. 17)
4. Align the clamping screw @ with the positioning hole @ on the light
exit, then tighten the screw to fasten the filter cassette.
+ When the filter cassette is installed, the Stage may hit it when
lowered. Therefore, exercise caution when lowering the Stage with
the filter cassette installed.
1 Using the Filter Cassette (Fig. 18) 1
45LBD-IF Color balancing filter
45ND-6, 45ND-25 Neutral density filter
45G-530, 456-533, 45lF550 Green
45Y-48 Yellow B & W contrast filter
450-560 Orange
45C-3, 45KB3 Daylight filter
Up to three of the above filters tan be inserted into the filter cassette.
Moving the levers @ on the left and right sides of the cassette to the IN
Position brings the corresponding filter into the light path.
Fig. 18
11 0

BX40
Fig. 19
Fig. 20
Fig. 21
Fig. 22
a
SpeMnefl Placement
(Figs. 19-21)
+ For Single slide observations, the maximum slide dimensions
are 26 x 76 mm, with a thickness of 0.9-1.4 mm and a cover
glass thickness of 0.17 mm.
+ When observing very large specimens, remove the slide holder
and place the specimen directly on the Stage.
1 Slide Holder for 2 Soecimens (Fis. 19) j
1. Turn the coarse adjustment knob @ to lower the Stage.
2. Open the spring-loaded curved finger @ on the slide holder and place
one or two specimen slides on the Stage from the front.
3. After placing the slides as far as they will go, gently release the curved
finger.
Slide Holder for Single Specimen (Figs. 20, 21) 5-l
=
The specimen tan easily be placed by sliding it into the slide holder 6
from the front. (Fig. 20) m
Using an Oil Immersion Objective
Adsorption of immersion oil tan Cause the specimen to drift. In such
cases, it is recommended to use the optional specimen Clip @
(BH2-SCB-3) for oil immersion objectives 0. (Fig. 21)
Q
Adjusting the Tension of the X-axis
and V-axis Knobs (Fig.221
The tension of the X-axis and Y-axis knobs tan be individually adjusted.
Turning the X adjustment ring @ or the Y adjustment ring @ counter-
clockwise increases tension, and turning them clockwise reduces
tension. When adjusting the tension, hold the X-axis and Y-axis knobs
to keep them from turning along with the tension adjustment rings.
+ If the tension is adjusted to tight, a creaking Sound may be
heard during Stage travel, and the Stage may backtrack.
0
12

Fig. 23
Fig. 24
Fig. 25
1. Slightly loosen the Stage clamping screw 0.
2. The Stage tan be rotated both to the left and to the right by turning it
with the Stage clamping screw.
Sr A click may be heard and felt during rotation. However, this is
due to the construction of the substage and does not indicate a
malfunction.
0 The angle of rotation varies depending on the Position of the Stage knobs.
Specifications are identical for both the Standard type and long
type knobs.
0 By lowering the Position of the substage, the microscope will
accommodate specimens with maximum heights of 40 mm. This is useful
when observing metallurgical specimens and other thick objects.
1. Lower the Stage to the lower limit, then remove the Stage from the
microscope. (See page 3)
2. Using the Allen screwdriver, loosen the substage bracket clamping screw
0 and remove the substage. (Fig. 24)
3. Turn the coarse adjustment knob and raise the focusing block @ to where
the stopper screw @ in the arm becomes visible. (Fig. 25)
4. Using the Allen screwdriver, loosen and remove the upper stopper screw
0.
5. Reattach substage bracket and Stage.
0 Store the removed stopper screw @ in a safe place so that it will not
be lost, if needed again.
13
0

Fig. 26
Fig. 27
Fig. 28
Fig. 29
While looking through the eyepieces, adjust for binocular Vision until
the left and right fields of view coincide completely. The index dot l
indicates the interpupillary distance.
0 Note your interpupillary distance so that it tan be quickly duplicated.
1, Looking through the right eyepiece with your right eye, rotate the coarse
and fine adjustment knobs to bring the specimen into focus.
2. Looking through the left eyepiece with your left eye, turn the diopter
adjustment ring (iJ to focus on the specimen. (Fig. 27)
( Usinn a Finder Evepiece 1
1, Looking through the right eyepiece with your right eye, turn the top of
the eyepiece @ until a clearly defined double crossline tan be seen in
the field of view. (Figs. 27, 28)
2. Looking through the right eyepiece, rotate the coarse and fine adjustment
knobs to bring the specimen and double crossline into simultaneous
focus.
3. Looking through the left eyepiece with your left eye, turn the diopter
adjustment ring @ to focus on the specimen.
a
4i3Q. 29)
1 When Wearing Eyeglasses
Use with the eye shades in their normal folded down Position. This will
prevent the eyeglasses from being scratched.
1 When Not Wearinn Evenlasses
Extend the folded eye shades in the direction of the arrow to prevent
extraneous light from entering between the eyepieces and eyeglasses.
0
14

Fig. 30
Fig. 31
Fig. 32
Eyepiece micrometer discs tan be inserted into WHIOX-H (and WHIOX)
eyepieces.
Use 24 mm diam. x 1.5 mm micrometer discs. Following Fig. 30, unscrew
the micrometer mounting frame @ from the eyepiece and place a micro-
meter disc @ into the mounting frame. The engraving on the micrometer
disc @ should face downward into the micrometer mounting frame 0.
Screw the micrometer mounting frame back into the eyepiece tube.
Slide the light path selector knob @ to select the desired light path.
0 The selector knob is ordinarily at the middle position. With dark specimens,
push the knob in. If additional light is needed for television or photo-
micrography, pull the knob out.
Pushed in 100% for binocular Observation of
eyepieces ( darkspecimens 1
20% for binocular Observationof
Middle Position LxlaxQ eyepieces, 80% brightspecimens.
photography,
for Wphotwwhy n/ obsep&ion
l I I l I l
Pulled out 1m fi / 100% for I Photography, /
lV/photography TV observatrons
l I / I I
Note: The U-llR is not equipped with a light path selector knob. The intensity
ratio of this Observationtube is 20% for binoculareyepieces, 80% for TV/
photography.
0 Adjust the height and tilt of the Observation tube to obtain the most
comfortable viewing Position. Holding the binocular section with both
hands, raise or lower it to the desired Position.
j, Never attempt to forte the binocular section past the upper or
lower stop positions. Applying excessive forte could destroy the
limiting mechanism.
+ When using the U-TBI-2 tilting Observation tube, only the viewing
attachments U-DO, U-MDO, U-SDO and the eyepoint adjuster U-
EPA tan be used. However, when using Plan20X (for a general
P-level configuration), illumination at the periphery will be slightly
insufficient and vignetting in the peripheral field may occur when
the apetture iris diaphragm is stopped down near its minimum
size. (If intermediate attachment combinations other than the
designated are used, illumination at the periphery will be slightly
insufficient.)
0 When using the U-TTR tilting Observation tube, the straight Photo tube
U-SPT or various Video adapters (with the exception of the double-port
U-DPT) tan be mounted on the trinocular Observation tube.
* The U-TBI tilting Observation tube is not recommended for use in
combination with intermediate attachments.
15 0