Olympus BX53M-P User manual

BX53/BX53M-P
This instruction manual is for the Olympus Polarizing Microscope. To obtain optimum performance and
to familiarize yourself fully with the use of your microscope, we recommend that you read this manual
together with the instruction manual for the BX53/BX53M microscope thoroughly before use. Retain this
instruction manual in an easily accessible place near the work desk for future reference.
INSTRUCTIONS
POLARIZING MICROSCOPE
A X 7 9 8 8
Optical Microscope and Accessory

BX53/BX53M-P
IMPORTANT.......................................................................................................................................................................................... 1
1 NOMENCLATURE...................................................................................................................................................................... 2
2 ASSEMBLY....................................................................................................................................................................................... 3
2-1 Assembly Diagram ..........................................................................................................................................................................................3
2-2 Detailed Assembly Procedure..............................................................................................................................................................4
3 CONTROLS................................................................................................................................................................................... 10
4 USING THE CONTROLS.................................................................................................................................................... 12
4-1 Stage ..........................................................................................................................................................................................................................12
4-2 Intermediate Polarizing Attachment..............................................................................................................................................15
5 POLARIZED LIGHT OBSERVATION.......................................................................................................................... 16
5-1 Adjustments Before Observation.................................................................................................................................................... 16
1Adjusting the Optical Axis............................................................................................................................................................... 16
2Adjusting for Extinction ....................................................................................................................................................................22
3Adjusting the Eyepiece Cross Lines .................................................................................................................................... 23
5-2 Orthoscopic Observation ......................................................................................................................................................................25
5-3 Conoscopic Observation.......................................................................................................................................................................26
6 SPECIFICATIONS .....................................................................................................................................................................27
7 OPTICAL CHARACTERISTICS......................................................................................................................................29
8 TROUBLESHOOTING GUIDE ........................................................................................................................................30
CONTENTS

1
IMPORTANT
If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition,
the equipment may also be damaged. Always use the equipment as outlined in this instruction manual.
The following symbols are used to set off text in this instruction manual.
: Indicates a potentially hazardous situation which, if not avoided, may result in minor or
moderate injury or damage to the equipment or other property. It may also be used to
alert against unsafe practices.
} : Indicates commentary (for ease of operation and maintenance).
CAUTION
1Getting Ready
1. A microscope is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact.
2. The BX53/BX53M microscope can be used with up to two additional intermediate attachments (e.g., U-DO3 dual-viewing
attachment, U-CA or U-ECA magnification changer, etc.). When using an additional intermediate attachment, please
make sure with your Olympus representative or the latest brochure.
3. Do not use the microscope where it is subjected to direct sunlight, high temperature and humidity, dust or vibrations.
Install it on stable, level bench. For operation environment conditions, see SPECIFICATIONS on page 28.
4. When disposing of the microscope, check the regulations and rules of your local government and be sure to observe
them.
2Caution

BX53/BX53M-P
2

*Components for polarized light observation
(Strain-free objective series)
*
*
*
Strain-free
condenser
BX53/BX53M
Microscope frame
Observation tube
Centerable revolving
nosepiece
Polarizing intermediate
attachment
Eyepiece
11NOMENCLATURE
*
*
Polarizing rotatable stage
Objective

3
22ASSEMBLY
2-1 Assembly Diagram
The diagram below shows how to assemble the various components. The numbers indicate the order of assembly.
} For details on the BX53/BX53M microscope frame, consult the BX53/BX53M manual.
When assembling the components, make sure that all parts are free of dust and dirt, and avoid scratching any
parts or touching glass surfaces.
CAUTION
Light source unit
Eyepiece
Centerable revolving
nosepiece (U-P4RE)
Test plate adapter (U-TAD)
Stage clips
Mechanical stage
(U-FMP)
Polarizing rotatable stage
(U-SRP)
Polarizing
intermediate
attachment
(U-CPA)
Strain-free condenser
(U-POC-2)
Microscope frame
(BX53F/BX53MTRF-S)
Intermediate attachment
for AN360P (U-OPA)
Rotatable analyzer
(U-AN360P)
Power cord
Observation tube
Sensitive tint plate (U-TP530)
Quarter wave plate (U-TP137)
Strain-free
objective
*6 types of compensators available

BX53/BX53M-P
4
2-2 Detailed Assembly Procedure
1Attaching the Stage (U-SRP) (Fig. 1)
1. Turn the coarse adjustment knob @ to lower the substage assembly ²
to its lower limit.
2. Loosen the centering knobs ³.
3. Position the stage with the vernier scale | in front, and carefully lower
the stage onto the round dovetail on the stage bracket ² with the
positioning pin on the stage aligned with the groove on the front of the
stage bracket, then loosely tighten the centering knobs.
Mounting Stage Clips and Mechanical Stage (U-FMP) (Fig. 2)
· Insert the stage clips securely into any two holes ƒ on the stage top
surface.
· Mount the mechanical stage so that the positioning pins on the un-
derside fit into the positioning holes … on the stage top surface. Using
the Allen screwdriver provided with the microscope frame, tighten the
clamping screw.
Fig. 1
Fig. 2

5
Fig. 3
2Mounting the Condenser (U-POC-2) (Fig. 3)
1. Turn the coarse adjustment knob @ to raise the stage to its upper limit.
2. Turn the condenser height adjustment knob ² to lower the condenser
holder to its lower limit.
3. Loosen the condenser clamping screw ³.
4. Position the condenser with the scale markings in front, insert it into the
substage fork as far as it will go.
Align the positioning pin at the back of the condenser with the groove
in the substage fork.
Swing the top lens out of the way before inserting the condenser.
5. Tighten the condenser clamping screw, then raise the condenser to its
upper limit.
CAUTION

BX53/BX53M-P
6
3Mounting the Objectives (Fig. 4)
Insert the 10X or 20X objective into the primary hole (position where black
rubber plugs are inserted into the centering holes on the nosepiece).
Mount the other objectives in such a manner, that the magnification in-
creases from low to high power in a clock-wise direction from the primary
hole.
Fig. 4
Low
Fig. 5
High
4
Mounting the Revolving Nosepiece (U-P4RE) (Fig. 5)
1. Turn the coarse adjustment knob to lower the stage all the way.
2. Using the Allen screwdriver, loosen the nosepiece clamping screw @
on the microscope frame.
3. Carefully slide the nosepiece along the dovetail to its limit, in the direction
of the arrow.
4. Clamp the nosepiece by tightening the nosepiece clamping screw.

7
5
Mounting the Intermediate Attachments (Fig. 6)
1. Using the Allen screwdriver, loosen the observation tube clamping screw
@ on the microscope frame.
2. Insert the circular dovetail mount at the bottom of the intermediate
attachments into the opening on the microscope frame and clamp by
tightening the clamping screw @.
When Using Intermediate Attachment for conoscopic and
orthoscopic observation (U-CPA)
Always make sure to mount this unit in such a manner, that it is
parallel to the arm.
Intermediate Attachment for orthoscopic observation (U-OPA)
} The position of this intermediate tube can be adjusted later. At this
point, place the tube so the nameplate is toward the rear.
CAUTION
FIg. 6