Olympus GX53 User manual

Instructions
Notes
This instruction manual is for the Olympus microscope.
To ensure safety, obtain optimum performance and to familiarize yourself fully with the use of this
product, we recommend that you study this manual thoroughly before operating this product, and
always keep this manual at hand when operating this product.
Retain this instruction manual in an easily accessible place near the work desk for future reference.
For details of products included in the configuration of this microscope, see page 10.
Inverted system metallurgical microscope
GX53
Optical Microscope and Accessory

In accordance with European Directive on Waste Electrical and Electronic Equipment, this
symbol indicates that the product must not be disposed of as unsorted municipal waste, but
should be collected separately.
Refer to your local Olympus distributor in EU for return and/or collection systems available in
your country.
NOTE: This product has been tested and found to comply with the limits for a Class A digital device,
pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection
against harmful interference when the product is operated in a commercial environment. This product
generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance
with the instruction manual, may cause harmful interference to radio communications.
Operation of this product in a residential area is likely to cause harmful interference in which case the
user will be required to correct the interference at his own expense.
FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance
could void the user’s authority to operate the product.
For Korea only
이기기는 업무용 환경에서 사용할 목적으로 적합성평가를 받은 기기로서 가정용 환경에서
사용하는 경우 전파간섭의 우려가 있습니다.
This product is applied with the requirements of standard IEC/EN61326-1 concerning
electromagnetic compatibility.
- Emission Class A, applied to industrial environment requirements.
- Immunity Applied to industrial environment requirements.
Some interference may occur if this product is used in domestic location.

GX53
Introduction....................................................................................................................................................... 1
Safety precautions...................................................................................................................................... 2
1 Nomenclature of units........................................................................................................................ 9
2 List of combinable units .................................................................................................................10
3 Basic operations of the microscope
(Reflected light brightfield/darkfield observations).................................................14
3-1 Reflected light brightfield/darkfield observation procedures....................................14
3-2 Turning ON the main switch....................................................................................................................16
3-3 Selecting the observation method....................................................................................................17
3-4 Selecting between the eyepiece light path and the camera light path ..........18
3-5 Placing a sample ..............................................................................................................................................19
1Placing a sample........................................................................................................................................................................ 19
2Moving the sample...................................................................................................................................................................19
3-6 Selecting the objective ...............................................................................................................................20
3-7 Focusing................................................................................................................................................................... 21
1Focusing.............................................................................................................................................................................................21
2Removing the fine focusing knob ...............................................................................................................................21
3Adjusting the tension of the coarse focusing knob....................................................................................21
3-8 Adjusting the brightness ...........................................................................................................................22
1When the reflected LED light source is combined......................................................................................22
2When the halogen lamp housing is combined ..............................................................................................22
3When the mercury lamp housing is combined...............................................................................................22
4When the light source is combined..........................................................................................................................22
3-9 Adjusting the observation tube...........................................................................................................23
1Adjusting the interpupillary distance........................................................................................................................23
2Adjusting the diopter...............................................................................................................................................................23
3Using the eye shades............................................................................................................................................................25
4Adjusting the tilting (U-TBI90 only)..............................................................................................................................25
3-10 Adjusting the aperture diaphragm (AS).....................................................................................26
Contents

3-11 Adjusting the field diaphragm (FS) ................................................................................................ 27
3-12 Using the filter sliders................................................................................................................................ 28
3-13 Observing the image using the mercury burner...............................................................29
4 Various observation methods...................................................................................................30
4-1 Reflected light differential interference contrast (DIC) observation.....................30
4-2 Reflected light simple polarization observation................................................................... 31
4-3 Reflected light brightfield/darkfield simultaneous observation..............................32
4-4 Transmitted light brightfield observation..................................................................................... 33
4-5 Transmitted light simple polarization observation ............................................................. 34
4-6 Using the analyzer/polarizer for reflected light observation.....................................35
4-7 Using the DIC slider.......................................................................................................................................36
1Inserting the DIC slider..........................................................................................................................................................36
2Adjusting the prism of the DIC slider........................................................................................................................37
4-8 Using the MIX slider for reflected light observation..........................................................38
1Inserting the MIX slider for reflected light observation.............................................................................38
2Turning ON the illumination of the MIX slider for reflected light observation.......................39
3Adjusting the brightness of the illumination .....................................................................................................39
4Selecting the illumination pattern................................................................................................................................40
4-9 Adjusting the units for transmitted light observation....................................................... 41
1Centering the condenser....................................................................................................................................................41
2Tilting of the illumination column.................................................................................................................................43
3Flipping up the condenser holder...............................................................................................................................43
5 Image acquisition................................................................................................................................44
6 Replacement of optical parts ...................................................................................................45
6-1 Replacing the eyepiece............................................................................................................................. 45
6-2 Replacing the objective ............................................................................................................................. 47
6-3 Replacing the halogen bulb...................................................................................................................48
6-4 Replacing the mercury burner .............................................................................................................50
6-5 Replacing the filter..........................................................................................................................................54

GX53
7 Troubleshooting....................................................................................................................................56
8 Specifications..........................................................................................................................................60
9 Optical performance list <<UIS2 series>> ..........................................................................62
10 Assembly..................................................................................................................................................65
10-1 Assembly diagram.......................................................................................................................................65
10-2 Assembly procedures............................................................................................................................... 66
1Attaching the light source ..................................................................................................................................................66
2Attaching the objective .........................................................................................................................................................68
3Attaching the revolving nosepiece.............................................................................................................................68
4Attaching the MIX slider / DIC slider for reflected light observation ...........................................71
5Attaching the stage..................................................................................................................................................................72
6Attaching the observation tube.....................................................................................................................................73
7Attaching the eyepiece.........................................................................................................................................................73
8Connecting cables.................................................................................................................................................................... 74
9Connecting the AC adapter and power cord...................................................................................................76
10 Attaching the ND filter for darkfield observation ............................................................................................77
11 Attaching the illumination column..............................................................................................................................78
12 Attaching the halogen bulb for transmitted light observation ..........................................................78
13 Attaching the lamp housing for transmitted light observation .........................................................78
14 Setting the filters.........................................................................................................................................................................79
15 Attaching the condenser.....................................................................................................................................................79
16 Adjusting the tension of the condenser height adjustment knob ................................................80
17 Attaching the scale slider...................................................................................................................................................80
18 Attaching and adjusting the camera adapter...................................................................................................81
10-3 Connection with PC.................................................................................................................................... 81
11 Preventive inspection sheet for illumination devices........................................82
12 Proper selection of the power supply cord...............................................................83

1
GX53
Introduction
Configuration of instruction manuals
Read all instruction manuals provided with the units you purchased.
The following instruction manuals are prepared for the units to be used with this product.
Manual names Main contents
Inverted system metallurgical microscope
(this instruction manual)
Reflected light brightfield observation, reflected light darkfield
observation, reflected light differential interference contrast observation,
reflected light simple polarization observation and transmitted light
illumination observation
BX3M-CB/CBFM
Control box/FM Control box Control box (BX3M-CBFM)
U-RFL-T power supply for mercury burner /
U-RX-T power supply for xenon burner Connection of the mercury lamp housing with the power supply
TH4 power supply for halogen bulb Connection of the halogen lamp housing with the power supply
GX-SLM Scale slider Scale slider (GX-SLM)
U-LGPS LED and LDP light source Connection of the LED and LDP light source with the liquid light guide, etc.
BX3M-PSLED LED power supply LED power supply for transmitted light illumination
This microscope employs the UIS2 optical systems. If you use an eyepiece, objective or condenser, etc. together with
this microscope, be sure to use those of UIS2 optical system series.
Using inappropriate units restricts the performance.
(There are other units usable with the UIS series. Contact Olympus or refer to the latest catalogs.)
Intended use
This product is designed to observe magnified images of samples for industrial applications.
Appropriate samples include semiconductors, electrical components, molded parts or mechanical parts.
Industrial applications include observation, inspection or measurements.
Do not use this product for any purpose other than its intended use.

2
Safety precautions
If the product is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the product
may also be damaged.
Read this instruction manual carefully and always use this product according to this instruction manual.
The following symbols are used in this instruction manual.
CAUTION : Indicates a potentially hazardous situation which, if not avoided, may result in
minor or moderate injury.
NOTE
: Indicates a potentially hazardous situation which, if not avoided, may result in
damage to the equipment or other property.
TIP
: Indicates the useful knowledge or information for use.
CAUTION - Installation of the product -
Install the product on a sturdy, level table or bench.
· If you install the microscope on an unstable table or a tilted table, the microscope may topple and you may get
injured.
· For safety, do not place a mat, etc. under the product.

3
GX53
Always use the AC adapter and the power cord specified by Olympus.
If the proper AC adapter, the power cord and cables are not used, the electric safety and the EMC (Electro-Magnetic
Compatibility) performance of the product cannot be assured. If no power cord is provided, please select the proper
power cord by referring to the section “Proper selection of the power cord” at the end of this instruction manual.
Always connect the ground terminal.
Connect the ground terminal of the power cord and that of the power outlet. If the product is not grounded, our
intended electric safety and EMC performance of the product cannot be assured.
Do not use the product in close proximity to the sources of strong electromagnetic radiation.
Proper operation may be interfered. The electromagnetic environment should be evaluated prior to operation of the
product.
Disconnect the power cord in case of emergency.
In case of emergency, be sure to unplug the power cord from the power cord connector on the product or from the
wall power outlet. Install the product at the location where you can reach the power cord connector or the power outlet
at hand to disconnect the power cord quickly.
This product is designed with intension to be used in the industrial environment for the EMC performance. Using this
equipment in the residential environment may affect other equipment.
CAUTION - Electric safety -
Do not look directly at the light from the LED lamp housing for a long time.
If you feel that the light from LED lamp housing is too bright during observation, adjust the light intensity using the
brightness control knob before continuing the observation. The LED built in this product is basically eye-safe. However,
do not look directly at the light from the LED lamp housing for a long time, since it may cause damage to your eyes.
Do not look directly at the light coming out from the objective or the specular reflection light from the
sample.
Do not look directly at the light coming out from the objective for a long time, since it may cause damage to your eyes.
Do not expose your skin to the light coming out from the objective for a long time.
If your skin is exposed to the light coming out from the objective for a long time, you may get burned.
a
CAUTION - LED (light emitting diode) -
Do not press the micro switch of the MIX slider for reflected
light observation (U-MIXR) with your finger.
If you press the micro switch aof the MIX slider for reflected light
observation with your finger, the MIX slider for reflected light observation
may emit the light.
Do not insert the MIX slider for reflected light observation
(U-MIXR) upside down.
Do not insert the MIX slider for reflected light observation upside down.
Otherwise, the light coming out from the MIX slider for reflected light
observation enters your eyes through the eyepiece and your eyes may
be damaged.

4
CAUTION - Lamp (halogen bulb and mercury burner) -
Remove the power cord from the product when replacing the
lamp.
To avoid electric shock hazards and burns when replacing the lamp, set
the main switch ato (OFF), and disconnect the power cord from the
product in advance. When you replace the lamp immediately after use of
the microscope, wait until the lamp housing and the lamp are sufficiently
cooled down.
Applicable lamp
[For U-LH100L-3]
12V100WHAL-L (made by PHILIPS Co. 7724I)
12V100WHAL (made by PHILIPS Co. 7023)
[For U-LH100HGAPO, U-LH100HG]
USH-103OL (made by Ushio Inc.)
Secure the sufficient space around the lamp housing.
The surface of the lamp housing on the back of the microscope gets
very hot. Therefore, when installing the microscope, secure the sufficient
space around the lamp housing (100 mm or more), particularly on the
top surface and bottom surface. After the microscope is installed, make
sure that the space below the lamp housing is sufficiently secured.
· As the UV ray of the mercury burner is harmful to your eyes, do not
light the mercury burner when the lamp housing is not attached to the
microscope.
· The lifetime of the illumination device is influenced significantly by
operating conditions (ambient temperature/humidity, voltage of the
power supply, lighting time, etc.), but is approximately eight(8) years
or 20,000 illumination hours if operating it 8 hours a day, whichever is
shorter, as a rough guideline.
· Since the lamp generates a high heat, perform the inspection
according to “11 Preventive inspection sheet for illumination devices"
on page 82. If you find the unexpected phenomena, e.g. smoke, etc.
during operation, turn OFF the power immediately and contact
Olympus.
a
Do not use the liquid light guide that is not conformed to this product.
You may get burned or your eyes may be damaged. For the liquid light guide that is conformed to this product, see
page 60.
Do not remove the liquid light guide when the lamp is turned ON.
The light emitted from U-LGPS may cause a fire. In addition, you may get burned or your eyes may be damaged.
Remove the liquid light guide after the liquid light guide and the LDP light source are fully cooled
down.
Otherwise, you may get burned.
CAUTION -
Liquid Light Guide -

5
GX53
CAUTION - Prevention of electric shock -
Never insert any tools or metal fragments in the air vents of the product.
It could cause electric shock or failure of the product.
Do not bend, pull or bundle the power cord and cables.
Otherwise cords and cables could be damaged, causing a fire or an electric shock.
Keep the power cord and cables sufficiently away from the lamp housing.
If the power cord and cables contact a hot area of the lamp housing, they could melt and cause electric shock.
Do not touch the product with wet hands.
In particular, if you touch the main switch of the power unit or the power cord with wet hands, electric shock, ignition or
failure of the product may be caused.
CAUTION - Prevention of injury
Be careful not to drop the glass plate.
If you are using the glass pate, be careful not to drop the glass plate. If the glass plate drops and is broken, you may
get injured by the broken pieces.
CAUTION - Prevention of fire -
Do not repair, disassemble or remodel.
Never repair, disassemble or remodel this product. Otherwise, fire may result. The repair work must be carried out only
by those authorized by Olympus.
If you need repairs, contact Olympus for assistance.

6
The following symbols are placed on this product.
Study the meaning of the symbols and always use the product in the safest possible manner.
Symbol Meaning
Indicates a non-specific general hazard. Follow the description given after this symbol or in the
instruction manual.
Indicates that the surface becomes hot, and should not be touched with bare hands. It may
cause burns.
Indicates high voltage. Take special care to prevent electric shock.
Indicates that the seesaw type main switch is ON.
(Seesaw type is the type of switch where ON or OFF is selected by pressing it to ON or OFF side.)
Indicates that the seesaw type main switch is OFF.
When caution labels are dirty or peeled off, contact Olympus for replacement or inquiries.
Label position and instruction
The labels are attached to the portions which require special cautions during use and operation. Be sure to follow these
instructions.
Label position Label Instructions in instruction manual Page
Left side on rear of microscope frame
External power supply for halogen bulb (TH4-100/TH4-200)
Control box (BX3M-CBFM)
Relay unit for coded revolving nosepiece (GX-IFRES)
Coded function system (U-CBS)
Electric safety page 3
Caution tag provided with microscope frame
Cautions for darkfield observation
and differential interference
contrast observation
page 77
Lamp housing mount on rear of microscope frame
Adapter for mercury lamp housing (MX-HGAD)
100W halogen lamp housing (U-LH100L-3)
High temperature page 4
100W mercury lamp housing (U-LH100HG)
100W mercury apo lamp housing (U-LH100HGAPO)
High temperature page 4
Electric shock page 5
LED and LDP light source(U-LGPS)
Electric safety page 3
Electric shock page 5
High temperature page 4
ND filter slider (U-25ND25-25/U-25ND25/U-25ND6)
Frost filter slider (U-25FR)
Interference contrast filter slider (U-25IF550)
L42 filter slider (U-25L42)
Light balancing filter slider (U-25LBD)
Yellow filter slider (U-25Y48)
Empty slider (U-25L42)
Light balancing amber filter slider (U-25LBA)
High temperature page 54
CAUTION - Safety symbols -

7
GX53
Handling Precautions
·This product is a precision instrument. Handle it with care and avoid subjecting it to a sudden or severe
impact.
·Never disassemble any part of the product. Otherwise, failure may result.
·Do not use the product in areas where it may be subjected to direct sunlight, high temperature and/or
humidity, dust or vibrations.
(For conditions of operating environments, see “8 Specifications" on page 60).
·Before attaching or detaching the units other than the MIX slider for reflected light observation
(U-MIXR), set the main switch of the microscope to (OFF).
·If units are not attached, be sure to attach the caps that are attached to the unit mounts to prevent
from dust.
·When observing a heavy sample exceeding 1 kg or using this product on a desk subject to external
vibration, if the vibration bothers you, use the anti-vibration table.
·Remove the pad for protecting the focusing unit that is attached as factory default. For removing
procedures, see “Attaching the stage" on page 72.
NOTE
CAUTION - Carrying the product -
Remove samples and units attached.
1. Make sure that the stage and revolving nosepiece are attached
securely. If the illumination column is attached, make sure that the
illumination column in tilted status is secured.
2. Be sure to remove samples and units, such as observation tube,
camera adapter and lamp housing, etc.
If you carry the microscope with units attached, the weight is
increased and the risk of dropping the microscope may be caused.
Be sure to carry the microscope by two persons.
When carrying the microscope, be sure to carry it by two persons. One
person holds the front holding section aand the other person holds
the rear holding section bas shown in the picture on the upper left.
If the illumination column for transmitted light observation is attached,
hold the holding section cas shown in the picture on the lower left.
Do not slide on the top surface of the table.
Do not move the microscope by sliding it on the top surface of the
table. The rubber legs may be damaged.
If you need to pack this product for shipping to
a distant location, etc., the dedicated transport
tools and packaging materials are necessary.
Be sure to contact Olympus for assistance.
c
a b

8
1. Do not leave stains or fingerprints on the lenses or filters. If they get dirty, blow away dust with a commercially
available blower and gently wipe the lens or filter with a piece of cleaning paper (or clean gauze).
Only when cleaning fingerprints and oil stains, slightly moisten a piece of cleaning paper with commercially available
absolute alcohol and wipe them off with it.
Since the absolute alcohol is highly flammable, it must be handled carefully. Be sure to keep it away
from open flames or potential sources of electrical sparks. For example, the electrical equipment that
is switched ON and OFF may cause the ignition of a fire. Also, always use absolute alcohol only in a
well-ventilated room.
2. Wipe the portions other than lenses with a dry soft cloth. If the dirt cannot be removed by dry-wiping, moisten a soft
cloth with diluted neutral detergent and wipe the dirty surface with it.
Do not use the organic solvents because they may deteriorate the coated surface or plastic parts.
3. After using this product, be sure to set the main switch to (OFF), wait until the lamp housing is cooled down sufficiently,
and keep it covered with a dust cover during storage.
4. Before disposing of this product, be sure to check the regulations and rules of your local government and follow them.
Contact Olympus for any questions.
5. If the hour counter of the power supply (U-RFL-T) shows 300 hours, set the main switch to (OFF) for safety purpose,
wait 10 minutes or more and replace the lamp. (See page 50.)
The mercury burner contains high-pressure gas in the tube. If you keep using the mercury burner
exceeding its lifetime significantly, the distortion accumulates in the glass tube to cause the explosion
in rare cases.
In case that the mercury burner explodes
Follow the procedures below in case that the mercury burner explodes.
· Unplug the power cord from the outlet. Leave the place and ventilate for at least 30 minutes.
· After the burner and the lamp housing have cooled down, collect the remaining mercury with packing tape,
paper or dropper, etc.
· Seal the collected mercury and used tools completely in a nonmetallic container, and ask the waste disposer
to dispose of them.
· If there is any possibility that you have inhaled mercury steam, consult the doctor immediately and follow his/
her instructions.
6. The used mercury burner must be disposed of as industrial waste. If you cannot dispose of it by yourself properly,
contact Olympus for assistance.
CAUTION
NOTE
NOTE
Maintenance and Storage

9
GX53
1
1Nomenclature of units
The diagram shown in this section shows the major units only. For units combinable to this product, see “2 List of
combinable units".
For units marked “*”, refer to the instruction manuals provided separately.
Observation tube
Stage
Revolving nosepiece
Stage center plate
Objective
Light source for
reflected light
illumination
Microscope frame
Polarizer
Analyzer
Scale slider*
Eyepiece
Scale slider
*
Scale slider*Scale slider
Analyzer
Front port of microscope frame
Intermediate attachment*
Eyepiece
Revolving nosepiece
Revolving nosepiece
Intermediate attachment*
Filter slider
Objective
Filter slider
Objective
Polarizer
Polarizer
Polarizer
Objective
Light source for
reflected light
illumination
Microscope frame

10
: Combination available (including units with restrictions)
: Combination prohibited
: Unnecessary for observation
Observation method
Unit
Reflected light Transmitted light
Brightfield Darkfield
Brightfield/
darkfield
simultaneously
Simple
polarization
Differential
interference
contrast
Brightfield Simple
polarization
Microscope frame GX53F
Observation tube U-BI90
U-BI90CT
U-TBI90
U-TR30H-2
Intermediate
attachment
*Only one type is usable.
U-CA
U-ECA
U-EPA2
GX-SPU
IX-ATU
Illuminator
Option
U-DULHA
MX-HGAD
U-LLGAD
Light source for
reflected light
illumination
BX3M-LEDR
U-LH100HGAPO
U-LH100HG
U-LH100L-3
U-LGPS
Transmitted light
illumination
IX2-ILL100
PMG3-LWCD
Light source for
transmitted light
illumination
BX3M-LEDT
U-LH100L-3
Power supply U-RFL-T
TH4-100
TH4-200
BX3M-PSLED
Light sources U-LLG150
U-LLG300
U-RMT
U-CST
U-CLA
22List of combinable units

11
GX53
: Combination available (including units with restrictions)
: Combination prohibited
: Unnecessary for observation
Observation method
Unit
Reflected light Transmitted light
Brightfield Darkfield
Brightfield/
darkfield
simultaneously
Simple
polarization
Differential
interference
contrast
Brightfield Simple
polarization
Revolving nosepiece U-P4RE
U-P5BDRE
U-P6RE
U-5RE-2
U-D6RE
U-D6RE-ESD-2
U-D7RE
U-5BDRE
U-D5BDRE
U-D6BDRE
Revolving nosepiece
(Coded type)
U-5RES-ESD
U-D5BDRES-ESD
U-D6RES
U-D6BDRES-S
U-D7RES
Objective adapter BD-M-AD
Stage GX-SFR
IX2-SFR
GX-SVR
IX2-GS
IX-SVL-2
Stage
option
CK40-CPG30
IX-CP50
IX2-GCP
GX-CP
Slider for reflected light
illumination
U-25ND6
U-25ND25
U-25ND50
U-25LBD
U-25IF550
U-25Y48
U-25L42
U-25FR
U-25LBA
U-25
DIC slider U-DICR
U-DICRHC
U-DICRHC

12
: Combination available (including units with restrictions)
: Combination prohibited
: Unnecessary for observation
Observation method
Unit
Reflected light Transmitted light
Brightfield Darkfield
Brightfield/
darkfield
simultaneously
Simple
polarization
Differential
interference
contrast
Brightfield Simple
polarization
MIX slider for reflected
light observation
U-MIXR
U-MIXRCBL
Polarizing element GX-PO
GX-POTP
GX-AN
GX-AN360
Filter slider GX-FSL -
Φ25 mm filter 25LBD
25ND6
25ND25
25FR
25IF550
Φ45 mm filter U-POT
43IF550-W45
45-LBD-IF
45-ND6
45-ND25
Scale slider GX-SLM
GX51-SLMG5
GX51-SLMG10
GX51-SLMG20
GX51-SLMG50
GX51-SLMG100
GX51-SLMGS
GX51-SLMGH
GX-SLM
Mirror unit CK40M-MS
Camera adapter U-TV1XC
U-TV0.63XC
U-TV0.5XC-3
U-TV0.35XC-2
U-TV0.25XC
U-CMAD3
U-TV1X-2
GX-TV0.5XC
GX-TV0.7XC
U-FMT
U-CMT
IX-TVAD

13
GX53
: Combination available (including units with restrictions)
: Combination prohibited
: Unnecessary for observation
Observation method
Unit
Reflected light Transmitted light
Brightfield Darkfield
Brightfield/
darkfield
simultaneously
Simple
polarization
Differential
interference
contrast
Brightfield Simple
polarization
Controller DP2-SAL
Hand switch BX3M-HS
U-HSEXP
TH4-HS
Control box BX3M-CBFM
U-CBS
GX-IFRES
Eyepiece WHN10X
WHN10X-H
CROSSWHN10X
Objective See “9 Optical performance list <<UIS2 series>>" on page 62.

14
This section describes the operating procedures of the reflected light brightfield/darkfield observations which are the
basis of observation methods. The polarization observation and the differential interference contrast observation, etc. are
described in “4 Various observation methods" on page 30.
3
Select the observation light path.
4Place a sample on the stage.
5Engage the 10X objective in the light path.
6
Bring the sample into focus.
Stage plate P.19
X-axis/Y-axis knobs P.19
Revolving nosepiece P.20
Coarse/Fine focusing knobs P.21
Brightness control knob P.22
Interpupillary distance adjustment scale P.23
Diopter adjustment ring P.23
Revolving nosepiece P.20
Coarse/Fine focusing knobs P.21
Aperture diaphragm lever P.26
Field diaphragm lever P.27
Filter slider insertion slot P.28
Brightness control knob P.22
Main switch P.16
11
Adjust the aperture diaphragm and
the field diaphragm.
(Fully open the aperture diaphragm during darkfield observation.)
12 Insert the required filters.
13 Adjust the brightness.
7
Adjust the brightness.
10 Engage the objective with the magnification
to be used in the light path and
bring the sample into focus.
Observation
Remove the analyzer, polarizer or
filter sliders from the light path.
BF/DF selection lever P.17
2
Select the brightfield (BF) or
darkfield (DF) observation.
3-1 Reflected light brightfield/darkfield observation procedures
Light path selection knob on trinocular tube
or Observation/SP selection knob on the
side port
1
Set the main switch to (ON).
Eyepiece/camera light path selection
knob P.18
Operation part Page
8
Adjust the interpupillary distance.
9
Adjust the diopter
3
3
Basic operations of the microscope
(Reflected light brightfield/darkfield observations)