Olympus VANOX User manual

OLYMPUS UNIVERSAL RESEARCH
MICROSCOPE
MODEL
INSTRUCTION MANUAL
1

Olympus Universal Research Microscope
Model VANOX
Table of Contents
I. System Chart af Various Units ........... 2
I I . Standard Equipment ................... 4
I II . Specifications ......................... 6
I . Principal Components and Light Path Diagram ... 7
V . Identification of Microscope Components ..... 8
A . Microscope Stand ................... 8
B . Binocular Observation Tube
with Photo Tuba ... 9
C . Revolving Nosepiece ................. 10
D . Square Mechanical Stage with low Drive
Controls 10
E . Achmmatic/Aplanatir: Condens~r ........ 11
...................
F . Condenser Mount 11
...............
.
G Auxiliary Lens System 11
H . Light Source ..................... 12
... .
VI A~qernbly Diagram and Instrument Aswmhly 13
.................
VI I . Operating the Microscope 16
...............
A. EImrica! hnnecticrn 16
...............
B. Center the Light Bulb 17
...............
.
C Center th~! Condenser 17
...
.
0 Operation of the Auxiliary Lens System 18
.......................
E . Condenser 19
.................
F . Focusing Procedure 20
...............
.
G Use of Iris Diaphragms 21
H . Operation of the Binocular Observation
Tube with Photo Tube 22
...
.
1 Use of Immersion Optical Components 23
.....................
VI I I . Photomicrography 23

I.
System Chart of Various Units
The various units in the chart below are based on the modular building-block system
of interchangeable accessories for a wide range of applications. The standard cam-
ponents are linked by bold lines. The optional accessories are linked by fine lines.
Super Wide field Binocular Tube
Binocular Tube with Photo Tub
with Photo Tube
I
P
Analyzer
Revolv~ ng Nosepiece
Vertical 11 lurninator Filter Turret Vertical I I luminazor for
for Brightf ield Bright
a~d Darkfield Fluorescence
Vertical
l lluminator
Stage Insert for Biological Work
M~tal slid^! Plate for Reflected Light,
Vertical Illuminator for Phase
Contrast Attachment
~omarski* Reflected Light
13 ~fferentiaI Interference
Stage Insertfor
Contrast A ttachrnent Fluorescence Work
I
Circular Rotatable
I
RotatableSquare
Polarizing
Stage Mechanical Stage
with Low Dr~ve
Cross Movement Controls
Mechamical Stage
I
~chromatict 1 mrners~on Fluorescence
Aplanatic SwinQ-aut Super Widef ield Polarir ing Dry Darkfield Darkf ield Phase Contrast Phas Contrast
Condenser Co ndencer Condenser Condenser Condenser Condenser Condenser
Auxiliary Lens System Condenw Mount I
Counterweight
Block

-
Viewing Screen
Attachment
Projection screen
Barrier Filter
Attachment
Photomicrographic
System Camera
Fluorescence
Magnification Light
Source
Changer
Nomarski Transm ~tted Light Differential
Interference Contrast Attachment
Auxiliary Collector
Lens
for Fluorescence
Microscope
Stand
Nomarski Transmitted
Light Differential
l nterference Contrast
Condenser
Polariter
Halogen L~ght Source
for Transmitted and
Reflected Light
*"Sub-Licenced from Union Optical CO!'

I I. Standard Equipment
Before assembly, please check your standard outfit, which comprises the following
items:
Microscope Stand 1
Binocular Tube with Photo Tube 1
Revolving Nosepiece 1
Rotarable Square Mechanical Stage with
Low Drive Cantrals
Condenser Mount 1
Auxiliary Lens System 1
Tungsten Lamp Mouse 1
Lamp Socket 1
Collector (transmitted light) 1
AchromaticlAplanatic Condenser 1
Centering Frosted Glass 1
Objectives: Plan 4X
Plan 10X
Plan 20X
Plan 40X 1
Plan 100X 1
Eyepieces; BiWF IQX, paired 1
Orthochromatic filters : E-533 (Green) 1
Y-48 {Yellow)
C (Cobalt)

Bulb (6V 5A)
Immersion oil (bottled)
Dust Cover
Optional Accessories
1.
Phase Contrast Attachment Model A-PC
2. F luorescence I I luminator Attachment Model A-F L-2
3. Fluorescence Phase Contrast Attachment Model A-F LPC
4.
Polarizing Attachments for Transmitted Light Models A-P-1 & A-P-2
5. Vertical lilluminater Model A-M
6.
Incident Phase Contrast Attachment Model A-MPC
7. Vertical Illuminator for Bright and Darkfield Model A-RLB
8. Halogen tight Source for Transmitted Light Model A-LSH
9.
Halogen Light Source for Reflected Light Model A-M-LSH
10.
Nomarski* Reflected Liqht Differential 3 nterferenceContrast Attachment Model
A-M-N I C
11. MagnificationChangerforTransmittedLightModelA-CA
12.
Low Power Condenser N.A. 0.06 Model A-ULC
13.
Dry Darkfield Condenser N.A. 0-810.92Model A-DCD
1
lmmersion Darkf ield Condenser N.A. 1.4/1.2 Model A-DCW
15. Swing-out Condenser N.A. 0.85 Model A-SC
16. Super Widefield Attachment Model A-SW
17. F Buoresence Vertical I Iturninator Attachment Mode! A-R F L
18.
Nomarski Transmitted Light Differential InterferenceContrast Attachment Model
A-NIC
*
"Sub-Licenced from Union Optical Co."

Ill. Specifications
Microscope Stand :
Standard Observation
Tube:
Revolving Nosepiece:
Mechanical Stage:
Condenser:
Condenser Mount:
Light
Source:
Auxiliary Lens System:
Height position of observation tubes is variable.
Various types of light sources are attachable.
Focusing: Vertical
stage movement.
Coaxial
coarse and fine adjustment knobs, with
automatic
pre-focusing lever.
Coarse Adjustment : Dovetail slideways, rackand-
pinion type, adjusfment range 49mm.
Fine Adjustment: Roller guide, gear train type;
adjustment range 2.3mm, graduated in increments of
1 micron. ,
Binocular observation tube with photo tube, with
constant
tube length adjustment. Tube inclination
30". Three-position light path selector lever. A
choice of intermediate adapters for special methods
of observation is available. Interpupillary adjustment:
54-74rnm. Field of view eyepieces for photo-
micrography
are available.
Quintuple,
on ball bearings.
Square stage (170 X 172rnrn), with low positioned
coaxial control knobs. Rotatable. Rack-and-pinion
drive. Movements on ball races, for both vertical and
horizontal excursions. Working range : vertical 52mm,
horizontal 76mm. Vernier scales reading to 0.1 mm.
Adjustable and removable specimen holder; inter-
changeable
stage inserts.
Aplanatic/achromatic condcnscr, N. A. 1.40, with
decenterable
aperture iris diaphragm and graduated
sale.
With condenser
centering dcvicc and rack-and-pinion
height adjustment.
Height displacement 30rnrn.
Tungsten lamp, 6V, 5A, w~th bulb centering device.
Bui It-in collector, heat absorbing filter and transformer,
variable 0-9.5V. Slot for insertion of light f~lters.
Flip-over type. Fitted with field iris diaphragm. For
optimum illumination from low power to oil immersion
objectives. Flips
180°. Knurled ring for operation
of field iris diaphragm.

IV. Principal Components and Light Path Diagram
Eyepiece
Photomicrographic
Sysfem Camera
i
B~nocular Observation
Tube w~th Phnta Tube
7 Microscope Stand
Revotvi nq Nosepiece
Conrrul Un~t
Aux~llary L~ns System
I
Swing-out Auxiliary Lens

V. ldentif ication of Microscope Components
A. Microscope Stand
a
Selector Turret for height adjustment of observation tube. This turret
ensures
correct height positioning of the observation tube. It permits
vertical
tube adjustment in 3 positions and can be engaged in 3 positions,
depending on the choice of accessories, as listed below.
@ @I Tube Clamping Levers. Use lever @ when the selector turret is in position
"S", and lever @ when the turret is in position "M. P" o~ "F. C".
@
Tube Dovetail Mount. The mount accepts various types of observation
tubes.
@
Stage and Condenser Mount Clampifig Block: This block accepts various
types of stages and the condenser mount.
@
Positioning Pin for Condenser Mount.
@ Coarse Adjustment Knob. Coarse adjustment range: 49mm.
Fine Adjustment Knob. Fine adjustment range: 2.3mm. graduated in
increments of 1 p.
@ Automatic ?re-focusing Lever. this lever prevents accidental damage to
specimen
or objective front lens.
(See page 20.)
@ Light Exit.
@
Filter Mount. The mount accepts various types of filters, auxiliary lenses,
centering frosted glass, etc.
@
Lever for Swing-out Frosted Glass.
Selector Turret
@
S
M. P
F. C
Application
Standard Microscopy
Metallurgical and
polarized
light microscopy
Fluorescent
light rnicro-
scopy, Magnification changer
Clamping
Lever to be
Used
0
0

Lever for Swing-out Auxiliary lens. Inserted into light path (pos. "'UL")
only when using very low magnification objectives (PI 1.3 x and PI 2 x).
Screw Mount for Transmitted Light Lamp House: The mount accepts
tungsten, fluorescent, and other light sources.
Positioning
Groove. To correctly orient the lamp house.
Opening
for Incident Light Source.
Voltage Adjustment
Knob. As sliding the knob toward the microscope
arm
along the slit, voltage rises.
The
knob must be set at the nearer end to you, when you turn on or off
the main switch.
MainSwitch (withbuilt-in pilot lamp)
Voltmeter
Output Socket
(to supply SOOVAC toextraelectric appliances)
Power frequency selection between 50Hz and 60Hz is provided ~nside the
base behind the SOOVAC output socket. When the Fnstrument is transferred
from the
50Hz area to the 60Hz area or vice versa, change the freq, selection
accordingly.)
Line Cord
Socket
GroundingTerminal
Line Voltage
Adjustment Screw
Low Voltage Outlet
6. Binocular Obsenration Tube with Photo Tuba
Eyepiece Tubes,
Diopter Adjustment.
lnterpupillary Distance Scale.
Light Path Selector Knob.
Indicator Platefor
Light Path Selector. (See gage 22.).
Eyepiece Tube for Photo Eyepieces.
Accepts F K photo eyepieces, designed
exclusively
for photomicrography.
Camera Ring Dovetail Mount. Accepts the Photographic Equipment
Model
PM-10-A.
Dovetail Slide.
Positioning Pin.
Nosepiece Dovetail Mount.
Accepts various types of revolving nosepieces
or intermediate adapters.

C. Revolving Nosepiece
a Clamping Screw.
@ Threaded Hole for Objective.
@ Knurled Ring.
@ Engravings. The engravings, coded A to E, are a guide for mounting
the objectives in ascending order of magnification.
@ Opening for Analyzer.
D.
Square Mmhanical Stage with Low Drive Controls
Specimen Holder. By loosening the clamping screw< @, th~! distance
between the holders can be adjusted or the entire holder can be removed.
Adjustment is made according to the size of th~! specimen slirl~
Clamping Screws for Specimen Holder.
North-South Movement
Knob. Range 52mm.
East-Wat Movement Knob. Range 76mm.
Graduated Scale for North-SouthMovement. With vernier reading to 0.1 mm.
Graduated Scale for East-West Movement. WFth vernier reading to 0.1 mm.
Clamping Screw for Stage Rotation.
Pin for Stage Rotation.
Dovetail Mount.
Clamping Lever.
Stage Insert. In addition to the standard insert (with longitudinal slot),
inserts
for
metallurgical, fluorescent and polarized light observations are
available.

E. Achromatic/ Aplanatic Condenser
@ Dovetail Slide.
@ Knurled Ring. Permirs adjustment of the aperture iris diaphragm.
@ Graduated Aperture Scale. The scale, engraved twice symmetrically,
indicates numerical aperture values
corresponding to the opening of the
aperture iris diaphragm.
@
Slide Lever.
This lever allows the center of. the aperture iris diaphragm
to be moved off the optical axis and rotated through 150°, thus obtaining
oblique illumination. (See page 19.)
@ Clamping Screw. Permits simultaneous locking of slide and rotation.
F. Condenser Mount:
G. Auxiliary Lenssystem
Dovetail for Condenser Slide.
Condenser Clamping Screw.
Condenser Centering Knobs.
Condenser
Height Adjustment
I<nob.
Dovetail Mount for Auxiliary
Lens System.
Mounting Bracket.
Clamping
Levcs.
Stop Pin for Auxiliary tens
Systcm.
a Mounting Dovetail.
@ Clamping Lever.
@
Stop Pin. {Provided beneath the
dovetail)
@
Lens Rotating Knob. The knob
is used to reverse the auxiliary
lens
system according to the
objective magnification in use.
@ Knurled Ring. To adjust the
@'
field iris diaphragm.

El. Light Source
Collector. Attached to lamp house by screw threads.
Knurled Ring.
To attach lamp house to the microscope stand.
Lamp House.
Filter Slot.
Ldmp Socket Clamping Screw.
Low Voltage Bulb, 6V 5A.
(See page 17.1
Lamp Socket.
Lamp Centering Knobs. Coaxial knob arrangement for bulb centration.
(See page 17.1
Positioning Pin. The pin correctly orients the lamp house to the microscope
stand.

VI. Assembly Diagram and Instrument Assembly
Eyepiece >
-
- --
Object Fve
r ...
I
Condenser Mount Ill
Condenser
Auxiliary Lens ~isxern
Microscope Stand #
4-A 1 Light Source

Remove dust caps before mounting various components.
All clamping levers have arrows indicating their clamping dire.ction.
1, Attachment of L~ght Source (Fig. 2)
lnsert the Fight source into the flange of the opening provided on the microscopestand,
aligning positioning groove
and positioning pin,and lock by turning the knurled clamping
ring.
2.
Attach the Condenser Mount. (Fig. 3)
First loosen clamping lever a , then fit the mounting bracket
of the condenser
mount correctly into the clamping block @ of the stand from above, slide the
condenser mount down all the way and lock with the clamping lever.
The arrow mark on the clamping lever shows the locking direction.
3.
Attach the Auxiliary Lens System.(Fig. 4)
Loosen clamping lever @ of the lens system, press the unit onto the dovetail slide
@ of the condenser mount, left side first and lock with clamping lever @.
Fig. 2 Fig. 3 Fig. 4
4.
Insert the Condenser. {F is. 51
Turn the arrow mark @ on top of the condenser slide towards the condenser mount
0, slide the condenser into the dovetail and lock with clamping screw @ .
* The condenser can also be inserted or removed with the mechanical stage in place.
In that case, lower the condenser with the condenser height adjustment until it can
easily
be removed without hitting the stage.
5. Mount the Mechanical Stage.
Insert the stage into the mounting block of the microscope stand in the same manner
as lhe condenser mount, slide down the stage all the way, and lock w~thclamping
lever
/Fig. 6). Stage insert and specimen holder are packed separately from the mechanical
stage; therefore,
attach the insert by aligning the positioning pin and groove, the
holder by inserting into the slide provided on the stage. (Fig. 7.8)
8 If the mechanical stage is intended to be used as a plain stage, the speclmen holder
need not be mounted.
Fig.
5 Fig. 6

6. Attach the Observation Tube.
11
First turn the selector turret on top of the microscope s?and to position "S".
2)
Check that the two clamping levers on the right hand side of the dovetail
mount are unclarnped (levers pointing upwards).
31
Insert the tube dovetail slide into the dovetail mount on the microscope stand
and lower the tube as far as possible.
4)
Lock the tube with the lower clamping lever. {Fig. 9)
* If the tube is used in standard position (selector turret at position "S"), be sure
to clamp the lower clamping lever only.
Fig. 7 Fig. 8 Fig. 9
7. Insert Revolving Nosepiece
11
Lower the stage as far as possible
2)
Unscrew the clamping screw @ of the nosepiece until it clears the thread @-
Pull spring-toaded clamping screw. This will cause the locating pin @ to withdraw.
(Fig. 10)
3)
With clamp~ng scrcw ,@ pullcd out, insert the nosepiece into the ring dovetail
@ on the tube, aligning locating pin @ with the locating groove @an ring
dovcta~l. Rclcasc clamping screw @ slowly and the locating pin will drive home
into the locating groove. Tighten clamping screw @ (Fig. 11)
* Locating groovc 10 and pin @ are in alignment if the nosepiece carrier stays
in
place when slightly moved to the right and left before tightening knob .
Fig. 10 Fig. 1 1
8. Mount the Objectives.
The objectives are mounted in ascending order of rnagnification according to the
engravings A to E on the nosepiece.
9. Insert the Eyepieces.
Insert
a pair of WFl OX eyepieces into the eyepiece tubes of the binocular observation
tube.
Use the field of view eyepiece for photomicrography only. {See page
24.)

V11. Operating the Microscope
It is good policy to keep the microscope irnmaculatel y clean. Remove visible spots,
specks
of dirt, dust or grease from all exposed glass surfaces.
A blower brush is a handy aid for this purpose.
How to put the microscope in operation:
1.
Switch on tungsten light source.
2. Center the light bulb. (See page 17. )
3.
Place a specimen slide on the mechanical stage.
4.
Push in the light path selector knob on the binocular tube all the way (white
band;
100% of the light is directed towards the eyepieces).
5. Make interpupillary and dioptric adjustments. {See page 22.)
6.
Swing in the desired objective.
7.
Select the auxiliary lens system according to the objective in use. (See page 18. )
8. Coarse focus with the coarse adjustment knobs.
9. Fine focus with the fine adjustment knobs.
10. Center the condenser. (See page 17.1
11.
Adjust aperture iris diaphragm and field iris diaphragm. (See page 21 .I
12. Adjust light intensity.
A. Electrical Connection
1 )
Insert the two plugs of the lamp cord into the lowvoltage outlet.
2)
Insert the plug of the line cord into the line cord socket.
3)
Make sure that the voltage adjustment knob is set at the nearer end to you
(low voltage), then push the main switch to supply AC pawer to the instru-
ment. When power is on, power pilot lamp lights.
* As you push the mainswitch again, the light is off, and all pawer is cut off.
4)
By vaising the voltage progressively, you can ascertain that the bulb is on.
Adjust light intensity to suit your requirement.
o Line Voltage Adjustment
1 ) Voltage Selector Switch
At thc bottom of thc base is a voltage selector switch, which can be turned
with
a coin, to correspond with the voltage of main supply (1 10V, 120V,
220V or 240V).
2) Line Voltage Adjustment Screw
Thc minimum voltage required for light source can be varied by means uf
the line voltage adjustment screw provided at the side of microscope base Ion
your left-hand side), since a silicon controlled rectifier ISCR) is adopted in
rhe dimmer circuitry.
If the bulb is dirnmly lit upon switching on light source, the line voltage is
proper, and
you have only to manipulate the voltage adjustment knob in order
to
obtain optimum light intensity. However, in case the bulb does not light
at all or does light up bright immediately after switching on, rotate gradually
the line voltage adjustment screw with a coin, until the bulb dims.
NOTE: For low voltage adjustment (0-9.5VJ after dimming the bulb, use
the voltage adjustment knob.
Voltmeter Reading
As the voltage adjustment knob IS moved along the slit, the red zone shifts as shown
schematically. The left-side picture indicates 4V, and the right-side picture 8V. Avoid
prolonged use of the light source at readingsin the red zone.
4-
16 8 -8

€3. Center the Light Bulb
1) First, make sure that the swing-out frosted
glass
at the Fight exit on the microscope base is
disengaged, and that the swing-out auxiliary
lens inside the base IS in the light path (lever
invertical position).
2) Insert thecenteringfrosted glassinto thefilter
mount
at the light exit.
3) Loosen the clamping screw of the lamp socket
m
F IS. 12
and move the socket back and forth to focus
the bulb filament on the frosted glass.
Adjust the light intensity, so that the
filament can be easily recognized. Clamp the lamp socket.
4)
Center the filament image with the two
coaxial centering knobs on the lamp
socket. (Fig. 12)
5) Removecenteringfrostedglass.
Repeat the above procedure after
installat~on of a replacement bu Ib.
Rulb Replacement Procedure
1) Loosen the sockct clamplng screw and sf~de out the socket.
21 Remove the bulb by slightly depress~ng ~t agalnst the wat and then rotating ~t In a counterclockw~se direct~on
31
Insert replacement bulb In rcvprsed order,
Before use, wipe off thororlghly any f~ngerprrnrs or stains on tne bulb
Fuse Replacement
At the back of the SOOVAC ourput sock~t, a fuse holder 1s posit~oned. When the fuse rs burned, slide the mlcroscope
to the desk edge on whlch the rnlcrwcope IS placed, unt~l there IF an openrng at the bottom of the microxope base
enouqh to realace rhe burned fu~e with a new one from 'beneath the bm Or lay Onwn the mlcroscope
* Remove the observat~on tube, stage, etc, prlor to replacement of the fuse
C. Center the Condenser
1)
The frosted glass at the light exit should be swung out of the light path
and the swing-out auxiliary lens should be engaged {lever in vertical
position}.
2) Push in the light path selector knob on the binocular tube all the way
(white band).
3) Place a specimen on the mechanical stage and use the objective 1bX to
bring
the specimen in focus.
4)
Raise the condenser all the way with the condenser height adjustment knob.
5) Swing the auxiliary lens system to position L and stop down the field
iris diaphragm with
the knurled ring. A slightly blurred image of the
field diaphragm can now be seen in the eyepiece.
6)
Move the condenser up and down to focus on the image of the field iris
diaphragm.
7) While widening the diameter of the field progressively, use the condenser
centering knobs to bring the diaphragm image into the center of the field
of vim. If the polygonal
image of the iris diaphragm becomes inscribed
in the field it means that the field diaphragm is centered. Slightly increase
diameter
of field iris diaphragm until it is just outside the field of view.

D. Operation of the Auxiliary Lens System - 1
The auxiliary lens system helps in achieving perfect
Koehfer illumination at all magnifications by manip- 0-
#
ulation of the auxiliary lens system @ , the frosted
glass @ , and the swing-out auxiliary lens @ , @ _ - -z+-mflv
eL- " +
respectively, Lens man ipulat~onis achieved by means
6
of levers or knobs as shown in the photograph.
/@
(Fig. 13) The correct lens positions for various
objective powers are summarized in the following:
Fig. 13
Condenser Frosted
Glass Lever
Objective Auxiliary
( Inser- Position
Lens
tion in70
Magnif i- Achromatic/-
Low
of Swing-
'
cation System
or remov- out
Aplanatic
power
I N.A.
1.4 al from Auxiliary
I
Light Lens
I Path)
------
I) I
4X
1 ox
VERTICAL
40X
1 OOX
* The objectives 1.3X and 2X and the low power condenser are optional
accessories .
Principle of Kaehler Illumination
Koehler illumination may be described as follows: "If an image
of a light source is conjugate
with the rear focal plane of an objective and the field iris diaphragm of the light source
is conjugate with the field ef view of the objective/eyepiece combination, maximum light
intensity
of the light source is obtained and the field of view is free from
'illurninatiorl
irregularities', irrespective of the shape of the light source," As shown in the drawing
below, the filament of the light source (6 V, 5A) forms, through the collector, an image at the
aperture iris diaphragm. This image, located at about the same place as the front focal point
of the condenser, is projected by the objective to the rear focal point
of the objective,
that is, in the exit pupil of the objective. Since this image is then projected Into the observer's
pupil, it is completely invisible to the observer, hence, illumination fre from 'irregularities'
is
obtained.
The field iris diaphragm, as will be noted from the
drawing, freely controls, by its opening and closing,
/ ~Aapa.pt.m i
the diameter of the cone of light entering the
Obletllvsrsarhlpdnt
condenser from the light source. spaam P~W ad thmd~mLgeof~lp19um
Correct Koehler illumination with the VANOX can
~~mdsy~ma~a~m-
be ob t'ai ned by appropriate positinning of rhe
auxiliary lens system and adjustment of aperture hx"mh6- -i--
FlsP I~d'sphrn
and field irisdiaphragms.