Olympus CHD User manual

scanned by 1. G. McHone 15 Jan 2010
for personal use only, not for sale
BIOLOGICAL MICROSCOPE
This instruction manual is for use of the Olympus Biological
Microscope
Model CHD. We recommend you read this
manual
carefully in order to familiarize yourself fully with
the use of your microscope so that you can obtain maximum
performance.
OLYMPUS
AX!)570

BEFORE USE
Observe the following procedures carefully:
a Operation
------------------------ __ ~---_-.I
CD Since the microscope is aprecision instrument, always handle it with care, and avoid abrupt motions or
impacts.
(i) Avoid exposure to direct sunlight, high temperature and humidity, dust and vibration.
@Never direct the sunlight to the microscope mirror while adjusting illumination in order to protect your
eyes from the sunlight.
@Only use the tension adjustment ring for altering the tension of the coarse adjustment knobs.
@ Be careful not to soil lens surfaces with dust, fingerprints, etc.
fJ Main18nance and .;;..Sto...;....ra _
CD Use aclean brush or lens tissue paper to clean the lens surfaces. If the lens surfaces are soiled with oil or
fingerprints, wipe them
off carefully with gauze moistened with asmall amount of alcohol and ether
(3: 7) solution, or xylene.
CV Do not use organic solutions (e.g. thinner, xylene, ether, alcohol, etc'! to wipe painted surfaces of various
components, and especially, plastic parts. When extremely soiled, they shou
Id be cleaned with aneutral
detergent.
@ Do not disassemble any part of microscope, since the integrated performance may be impaired.
@When not in use, the microscope should be covered with the dust cover provided or contained in astorage
case, and kept in aplace free from humidity and mold.
Attaching the fixing blocks of the microscope stand In the wooden ilqrege case ICHD-WBI
(optionally avail'able,
The fixing blocks should be screwed at the bottom of
the storage case in the following procedure:
1) Insert the screw into aflat washer and one of the
two holes (8 mm dia.) in the base plate of the case
as illustrated at the right.
2) Tighten the screw to clamp one block
by means of
the spanner provided.
3) Clamp the other block on the opposite side.
Fixing block
Storage
case
y-
Flat
washer
~
~

BEFORE USE
Observe the following procedures carefully:
a Operation
CD Since the microscope is aprecision instrument, always handle it with care, and avoid abrupt motions or
impacts.
CV Avoid exposure to direct sunlight, high temperature and humidity, dust and vibration.
@Never direct the sunlight to the microscope mirror while adjusting illumination in order to protect your
eyes from the sunlight.
@Only use the tension adjustment ring for altering the tension of the coarse adjustment knobs.
@ Be careful not to soil lens surfaces with dust, fingerprints, etc.
II Maintenance and Stora
CD Use aclean brush or lens tissue paper to clean the lens surfaces. If the lens surfaces are soiled with oil or
fingerprints, wipe them
off carefully with gauze moistened with asmall amount of alcohol and ether
(3: 7) solution, or xylene.
CV Do not use organic solutions (e.g. thinner, xylene, ether, alcohol, etc,) to wipe painted surfaces of various
components, and especially, plastic parts. When extremely soiled, they should
be cleaned with aneutral
detergent.
@ Do not disassemble any part of microscope, since the integrated performance may be impaired.
@When not in use, the microscope should be covered with the dust cover provided or contained in astorage
case, and kept in aplace free from humidity and mold.
Attaching the fixing blocks of the microscope stand In the wooden storage case ICHO·\)VB)
(optionally available)
The fixing blocks should be screwed at the bottom of
the storage case in the following procedure:
1) Insert the screw into aflat washer and one of the
two holes (8 mm dia.) in the base plate of the case
as illustrated at the right.
2) Tighten the screw to clamp one block
by means of
the spanner provided.
3) Clamp the other block on the opposite side.
Fixing block
Storage
case
I y-
Flat
washer
~
~

CONTENTS
1. STANDARD EQUIPMENT 1I
2. SPECIFICATIONS 22
3. NOMENCLATURE 33
ASSEMBLY 4
4:
4.
14-1
AS$embly' DIagram
41
14-~
Expranation for Assembly Pf'ocedIJre
'I
5.
OBSERVATION (Putting the Microscope in Operation) 6
DSpecimen Placement 6
fJ Adjustment of the Microscope Mirror 7
II Focus 7
II Interpupillary Distance Adjustment (for CH-BI45-W only) 7
II Diopter Adjustment 8
II Objective selection 8
II Adjustment of the Aperture Iris Diaphragm 8
.. Tension Adjustment of the Coarse Adjustment Knobs 9
III Locking of the Pre-focusing Lever 9
1m Use of Immersion Objectives
10
6. OPTICAL DATA
06
7. TROUBLE SHOOTING
11 "--_----I

I
--
I-
Z
w
~
a..
:::l
d
w
o
a::
«
o
z
~
en
STANDARD EQUIPMENT
CHO·
Component
212E
012E OOlE
--
Microscope stand
with
quadruple revolving nosepiece,
square plain stage, including dust plug AA7808,
fiIter CHO-F 00 0
32.5C-2, immersion oil Bcc and dust cover COll
Monocular observation tube, inclined 45° CH-M045-W 0 0
Binocular observation tube, inclined 45° CH-BI45-W 0
Attachable mechanical stage with right-hand low drive CH-MVR 00
controls
Stage clips, paired CH-SCBI 0
Condenser, N.A. 1.25 CH2-CO 0 0 0
Filter holder CH2-FH 00 0
Plano-concave mirror
K-MM 0 0 0
E 0 achromatic objective 4X
ED4X 0
E 0 achromatic objective
lOX
E010X
000
E 0 achromatic objective
40X (spring)
E040X/R
0 0 0
E 0 achromatic objective 100X (spring, oil)
E0100X/RO 00
LB eyepiece
lOX CWHK10X 00
LB eyepiece 10X(2 pcs.) CWHK10X 0
Optional accessories:
Phase contrast attachment
Simple
phase contrast attachment
Vertical
illuminator
Attachable mechanical stage with left-hand
low drive controls
Sub-stage
ilium inator
Table stand
illuminator
Wooden storage case
CH2-PCO-PL
CH2-PC-PL
BH2-KMA
CH-MVL
LSK-3
LSO-W
CHO-WB
D----------------------------

2
SPECIFICATIONS
Item Description
Microscope Circular dovetail mount for observation tube; built-on quadruple
nose-
limb piece; and square plain stage 124mm x153 mm.
Coarse and fine Coaxial
coarse and fine focusing controls.
Coarse and fine drive range
Microscope adjustments
25mm; fine drive knobs graduated in increments of 2.5J1..
Tension adiust-
stand ment knobs, and pre-focusing lever for
coarse focusing.
Condenser holder Rack
and pinion condenser height displacement up to 28mm.
Base
Provided with accommodation for attachment of microscope mirror and
substage illuminator.
Monocular Inclined 45°.
Observation Inclined 45°. Interpupillary distance adjustment
with a scale between
tubes Binocular
53mm -72 mm.
Variable diopter adjustment ring equipped on the left-side eyepiece tube.
Attachable mechanical
stage
Low-positioned coaxial
control knobs; X-V
trave~sing area 76mm x
50 mm, compatible
with two standard slides simultaneously.
N.A. 1.25 (in immersion oil).
with
graduated aperture diaphragm. Pro-
Condenser vision to accept afilter holder.
Filter holder Accepts a32.5
mm dia. filter.
Microscope mirror Plano-concave
mirror (50mm dia.l
Filter Blue
filter (32.5mm dia.)
Objectives
ED4X, EDlOX, ED40X (spring-loaded). ED100X (spring-Ioaded, oiIim-
mersion)
Eyepiece CWHK10X (provided
with accommodation to accept
an eyepiece micro-
meter)
Dimensions 180 mm
(W) x223 mm (D) x370 mm (H) (binocular version)
Eyepoint height Binocular: 372 mm Monocular: 375 mm
Weight CHD-212E: 4.9
kg.
--------------------------fJ
en
\l
m
("")
."
("")
!i
o
z
en

UJ
a::
::>
I-
«
...J
u
Z
UJ
~
o
z
3
NOMENCLATURE
Diopter adjustment ring
I Binocular tube
IRevolving nosepiece I
IObjective I
Specimen holder
I Mirror I
Hand reset
IStage clip I
Condenser height adjustment
knob
Pre-focusing lever
IEyepiece I
Observation tube
clamping screw
Mechanical stage I
IMicroscope stand
Tension
adjustment ring
Fine
adjustment knob
Graduated in increments of 2.5/J.
Coarse adjustment knob
Coaxial low drive control
knobs
I Monocular tube I
Condenser clamping screw
ICondenser I
Aperture iris diaphragm
lever
Filter holder
Accepts a 32.5mm dia. filter.
IJ-----------------------

4
--------
ASSEMBLY
[4-1 Assembl,V Diagram
*Assemble each component in the order of the numbers, taking care to keep all glass surfaces clean and avoid
scratching the lens surfaces,
Objective
~
V
S ta ge -------f
clip
Mechanical stage
Filter (32,5 mm dia.)
condenser.g
II 10
1
~
Filter
holder
..
Microscope
stand
II
»
(f)
(f)
m
s:
CD
~
4-2 Exp'lanation for Assembl,V Procedure
Fig. 1
DMounting the observation tube
1) Loosen the clamping screw CD fully, and mount the observation tube
on the circular dovetail mount. Reclamp the screw CD to securely hold
the observation tube on the stand. (Fig. 1)
2) The observation tube can be normally mounted on the stand with the
eyepiece tube(s)
pointing backward the microscope so that the observer
can look into the eyepiece(s) over the pillar to utilize available light as
much as possible; however, the observation tube can be clamped at any
direction to the observer preference or convenience.
IJ Insertion of the eyepiece(s)
Insert the eyepiece(s) into the eyepiece tube(s) (~). (Fig. 1)
-----------------------------11

II
Fig. 2
(J)
I Use of an eyepiece micrometer I
An eyepiece micrometer (10 mm/100~ (optionally available) can be
inserted into the eyepiece CWHK10X in the following procedure:
1) Remove the retaining
ring' 1./ from the lower end of the eyepiece
and place the micrometer on the retaining ring
with the reticle-
engraved surface, facing
downward. (Fig. 2)
* Be certain to clean the micrometer disc before inserting into the
eyepiece.
2) Return the retaining ring into the eyepiece and insert the eyepiece
into the eyepiece tube.
Fig. 3
Fig. 4
Fig. 5
III Mounting the objectives
1) Lower the stage by means of the coarse adjustment knobs.
2) Screw the objectives into the nosepiece, from low power to higher
power in aclockwise
direction. (Fig. 3)
*Close the empty aperture in the nosepiece with aplug CD provided.
(Fig. 3)
II Mounting the mechanical stage
Mount the mechanical stage on the plain stage CD in amanner that
the specimen traversing guide of the mechanical stage is located
closest
to the pillar, and tighten the stage clamping screws ® with
acoin. (Fig. 4)
•Insertion
of stage clips
The plain stage
is pre-drilled for insertion of the stage clips when the
mechanical stage
is not attached (see page 4).
II Mounting the condenser
Insert the condenser into the condenser holder, with the condenser
iris diaphragm lever
(1), directing in the microscope front, and
tighten the clamping screw (2) .(Fig. 5)
11----------------------------

II Insertion of the blue filter
Slip the blue filter (32.5mm dia.) ® into the filter holder CD. (Fig.
6)
II Insertion of the filter holder
Insert the filter holder m into the condenser from below. (Fig. 6)
5
Fig. 6
... Mounting the mirror
1) Insert the mirror C:D fork into the mirror mounting seat ®in the
base.
2) If the mirror fork is loose, pullout the mirror fork, and adjust the
slit in the
fork with ascrewdriver.
Fig.7
oBSE RVAT ION (Putting the Microscope in Operation)
o
OJ
(Il
m
:Xl
<
»
-I
(5
Z
Fig. 8
DSpecimen Placement
1) Open the spring-loaded specimen finger CD, and slide the specimen
slide
into the holder, with the cover glass upward.
Push the slide all the way against the holder and release the finger CD
slowly. (Fig. 8)
*A sudden release of the finger may cause damage to the slide or
the holder.
2) For use of the stage clips in place of the specimen holder, insert apair
of stage clips into the holes on the drilled surface of the stage and
insert
the specimen slide between each clip and the stage surface.
•
Use of acover glass
Use acover glass of 0.17mm thick in conjunction with the objectives marked with the inscription "160/
0.17" for optimum performance of these objectives.
•
Use of aspecimen slide
The thickness
of aspecimen slide between 0.9 mm and 1.2 mm is recommended for the CHD. If the thick-
ness of aslide exceeds this range, illumination may sometimes be impaired.
---------------------------B