Olympus BX43 User manual

INSTRUCTIONS
BX43
SYSTEM MICROSCOPE
This instruction manual is for the Olympus System Microscope Model BX43.
To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use
of this microscope, we recommend that you study this manual thoroughly before operating the
microscope.
Retain this instruction manual in an easily accessible place near the work desk for future reference.
A X 7 8 5 1

In accordance with European Directive 2002/96/EC on Waste Electrical and Electronic Equipment,
this symbol indicates that the product must not be disposed of as unsorted municipal waste, but
should be collected separately.
Refer to your local Olympus distributor in EU for return and/or collection systems available in your
country.
NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device,
pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection
against harmful interference when the equipment is operated in a commercial environment. This
equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in
accordance with the instruction manual, may cause harmful interference to radio communications.
Operation of this equipment in a residential area is likely to cause harmful interference in which case
the user will be required to correct the interference at his own expense.
FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance
could void the user's authority to operate the equipment.

BX43
CONTENTS
Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to assemble
the microscope yourself, please read section 10, “ASSEMBLY” (pages 33 to 37) carefully.
IMPORTANT -- Be sure to read this section for safe use of the equipment. -- 1-3
13-25
4
5-7
8,9
10-12
1 MODULE NOMENCLATURE
2 CONTROLS
3 FLOW OF OBSERVATION
4 SIMPLIFIED OBSERVATION PROCEDURE
4-1 Basic Operation (Until Observation of Specimen) ..................................................................... 10,11
4-2 Microscope Adjustments (How to improve the Observed image)..................... 11,12
1Adjusting the Interpupillary
Distance................................................................................11
2Adjusting the Left and Right
Focusing...............................................................................11
3Adjusting the Centering.................................12 4Adjusting the Contrast.....................................12
5 USING THE CONTROLS
5-1 Base..............................................................................................................................................................................................................13-15
1Adjusting the Brightness..............................13 2Setting the LIM Brightness.........................13
3Using the Filters (Halogen Bulb
Operation Only)..........................................................14
5-2 Focusing Block............................................................................................................................................................................... 16,17
1Focusing Controls..................................................16 2Adjusting the Focus.............................................16
3Replacing the Fine Adjustment
Knob............................................................................................17
4Adjusting the Coarse Adjustment
Knob Tension.................................................................17
5Pre-focusing Lever..................................................17
5-3 Stage............................................................................................................................................................................................................18-24
1Placing the Specimen......................................18
3Adjusting the X- and Y-Axis
Knob Tension................................................................19
4Rotating the Stage............................................... 20
5Adjusting the Stage Height..................... 20
5-4 Observation Tube................................................................................................................................................................... 21-24
1Adjusting the Diopter....................................... 21 2Using the Eye Shades.................................... 21
3Using the Eyepiece Micrometer
Disk............................................................................................ 22
4Selecting the Light Path of the
Trinocular Tube........................................................ 22
5Adjusting the Tilt..................................................... 23 6Using Eyepieces Incorporating a
Micrometer...................................................................... 24
5-5 Condenser................................................................................................................................................................................................... 24
1Compatibility of Objectives and
Condensers................................................................... 24
2Using the Auxiliary Slide Holder.........18

5-6 Immersion Objectives................................................................................................................................................................. 25
1Using Immersion Objectives................. 25
5-7 Objectives with Correction Collar.............................................................................................................................. 25
6 CAMERA RECORDING 26
PROPER SELECTION OF THE POWER SUPPLY CORD.......................................................... 39,40
7 TROUBLESHOOTING GUIDE
8 SPECIFICATIONS
9 OPTICAL CHARACTERISTICS (UIS2 Series)
10 ASSEMBLY -- See this section for the replacement of the light bulb. --
11 HALOGEN LAMP SOCKET INSPECTION SHEET
1System Chart......................................................... 26 2Selecting the Camera Adapter
Magnification......................................................... 26
27-29
30,31
32
33-37
38

1
BX43
Fig. 1
SAFETY PRECAUTIONS (Fig. 1)
IMPORTANT
1. After the equipment has been used in an observation of a specimen that
is accompanied with a potential of infection, clean the parts coming in
contact with the specimen to prevent infection.
· Moving this product is accompanied with the risk of dropping the
specimen. Be sure to remove the specimen before moving this
product.
· In case the specimen is damaged by erroneous operation, promptly
take the infection prevention measures.
· Follow the procedures described in Chapter “Getting Ready” (see on
page 2) prior to using the accessories of this product. Otherwise, the
stability of the device will be lost and the dropped specimen will cause
the possibility of infection.
2. Install the microscope on a sturdy, level table or bench.
3. Be careful not to tilt the microscope too much. Otherwise, the mobile
parts such as the light path selector knob may move in unintended
directions.
4. Always use the power cord provided by Olympus. If no power cord is
provided, please select the proper power cord by referring to the section
“PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of
this instruction manual.
If the proper power cord is not used, product safety performance cannot
be warranted.
5.
Always ensure that the grounding terminal of the microscope and that of the
wall outlet are properly connected. If the equipment is not grounded, Olympus
can no longer warrant the electrical safety performance of the equipment.
Designated bulb 6V30WHAL (PHILIPS 5761)
Always use the designated lamp bulb. Using other bulb may
cause malfunction.
2. If the lamp cable or power cord comes in contact with the lamp socket
or its surroundings, the cable or cord may be melt by the heat, causing
an electric shock or fire hazard. To prevent this, distribute the lamp cable
and power cord at enough distances from the lamp socket.
3. The standard service life of the lamp housing is eight (8) years of use or
20,000 hours of total power ON period, whichever is the shorter period.
For details, see Inspection Sheet on page 38.
Operation Using the LED Lamp
Operation Using the Halogen Bulb
1. To avoid potential shock hazards and burns when replacing the halogen
bulb, set the main switch of the TL4 power supply unit to “ ” (OFF) and
allow the lamp socket @ and bulb to cool before touching them.
Safety Note on LED (Light Emitting Diode)
The LED incorporated in the LED lamp housing is a class 1 LED
product as defined in the applicable standard. The light emitted by LED
is basically safe, but do not look at the illumination light directly for an
extended period to prevent your eye from being injured
CLASS 1 LED PRODUCT
1
CAUTION

2
Safety Symbols
The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment
in the safest possible manner.
Symbol Explanation
Indicates that the surface becomes hot, and should not be touched with bare hands.
Indicates a non-specific general hazard. Follow the description given after this symbol or in
instruction manual.
Indicates that the main switch is ON.
Indicates that the main switch is OFF.
Caution engraving/label
Caution engraving and label are placed at parts where special precaution is required when handling and using the
microscope. Always heed the cautions.
Caution engraving
position
Halogen lamp socket
[Caution against high temperature]
Caution label
position
Rear panel
[Caution against high temperature]
If a caution engraving or label is dirty or peeled off, contact Olympus for the replacement or other inquiry.
1 Getting Ready
1. A microscope is a precision instrument. Handle it with care and avoid
subjecting it to sudden or severe impact. Also note that the microscope
does not have a waterproof construction.
2. Do not use the microscope where it is subjected to direct sunlight, high
temperature and humidity, dust or vibrations. (For the operating conditions,
refer to section 8, “SPECIFICATIONS”.)
3. When moving the microscope, remove the observation and specimen
and carefully carry the microscope by holding the grasping part on the
rear of the arm and the front part of the base as shown in Fig. 2.
To prevent damage to the microscope, never hold it by the stage
or coarse/fine adjustment knob.
· Even when moving the microscope along the desktop surface, be sure
to remove the specimen in advance.
· When the microscope needs to be packaged for forwarding to a remote
location, contact Olympus.
4. When the accessories of this product are used, make sure that the system
height does not exceed 1 meter. Otherwise, the stability of the device will
be lost.
Fig. 2
CAUTION

3
BX43
2 Maintenance and Storage
1. To clean the lenses and other glass components, simply blow dirt away using a commercially available blower and wipe
gently using a piece of cleaning paper (or clean gauze).
If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available absolute
alcohol.
·Since the absolute alcohol is highly flammable, it must be handled carefully.
Be sure to keep it away from open flames or potential sources of electrical sparks --- for example, electrical
equipment that is being switched on or off, which could cause ignition of a fire.
Also remember to always use it only in a well-ventilated room.
·Set the main switch to “ ” (OFF) and wait for the lamp housing to cool down sufficiently before proceeding
to maintenance.
2. Do not use organic solvents to clean the microscope components other than the glass components. To clean them,
use a lint-free, soft cloth slightly moistened with a diluted neutral detergent.
3. Do not disassemble any part of the microscope except for the parts that are specified to be disassembled in this manual,
as this could result in malfunction or reduced performance.
4. After using the microscope, set the main switch to “ ” (OFF), (wait for the lamp socket to cool down sufficiently if the
halogen bulb has been used,) and keep it covered with a dust cover during storage.
5. Before disposing of this product, be sure to follow the regulations and rules of your local government.
3 Caution
If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition,
the equipment may also be damaged. Always use the equipment as outlined in this instruction manual.
The following symbols are used to set off text in this instruction manual.
: Indicates a potentially hazardous situation which, if not avoided, may result in minor
or moderate injury or damage to the equipment or other property. It may also be used
to alert against unsafe practices.
} : Indicates commentary (for ease of operation and maintenance).
4Intended use
This instrument has been designed to be used to observe magnified images of specimens in various routine work and
research applications.
Do not use this instrument for any purpose other than its intended use.
This instrument is considered a medical device in the following countries: US, and China.
CAUTION
CAUTION
This device complies with the requirements of directive 98/79/EC concerning in vitro diagnostic
medical devices. CE marking means the conformity to the directive.

4
1MODULE NOMENCLATURE
}The modules mentioned below show only the typical product names. As there are some products that are not mentioned
but also applicable to this microscope, check the latest catalogues or consult Olympus.
For the products marked “ *”, also read their instruction manuals.
Eyepieces
· WHN10X
· WHN10X-H
Revolving nosepiece
· BX43-5RES
· U-5RE-2
· U-D6RE
Stage
· U-SVRB-4
· U-SVRO
Observation tube
· U-BI30-2
· U-TBI-3
· U-TR30-2
· U-TTLBI
Intermediate attachment
· U-TRA
· U-CA
· U-DO3
Arm (or vertical illuminator)
· BX3-ARM
· BX3-URA*
· BX3-RFAS*
Lamp housing
· U-LHLEDC
· U-LS30-5
Condenser
· U-LC
· U-SC3
· U-AC2
Slide holder
· U-HLST-4
· U-HD-4
· U-HLDT-4

5
BX43
2CONTROLS
}If you have not yet assembled the microscope, read section 10, “ASSEMBLY” (pages 33 to 37).
Interpupillary distance adjustment
scale (Page 22)
Diopter adjustment ring
(Page 22)
Slide holder clamping lever
(Page 19)
Aperture iris diaphragm ring
(Page 12)
Condenser centering screws
(Page 12)
Filter mount (Page 15)
Field iris diaphragm ring
(Page 12)
Condenser height adjustment
knob (Page 12)
Allen screwdriver
holder
Y-axis knob (Page 20)
X-axis knob (Page 20)
Coarse adjustment
knob (Page 18)
Coarse adjustment tension
adjustment ring (Page 18)

6
Main switch (Page 10)
I: ON
: OFF
Fine adjustment knob
(Page 16)
Pre-focusing lever
(Page 16)
LIM indicator
ON: Lit (Green)
OFF: Extinguished
LIM ON-OFF switch
(Page 13)
LED brightness adjustment
knob (Page 13)
LIM setting switch
(Page 13)

7
BX43
Low Magnification Condenser
U-LC
LS30 Adapter
U-LS30ADP
Swing-out Condenser
U-SC3
Halogen Lamp Socket
U-LS30-5
Auxiliary lens mounting
tool (for BX53)
Auxiliary lens
*The auxiliary lens is mounted on the lamp adapter when the U-LC is used. (Page 34)
Top lens swing-out
knob
Top lens
Aperture iris diaphragm
ring (Page 12)
Condenser mount
dovetail
<<Modules for halogen lamp operation>>
Lamp cable
Power Supply Unit
TL4
Brightness control
knob
Main switch
I : ON : OFF
Lamp socket connector
Guide pins
Guide pin holes

8
3FLOW OF OBSERVATION
}When the LED lamp is used and the LIM is set, the LED brightness adjustment knob is defeated.
}When the halogen bulb is used, set the LBD filter in the filter mount on the base (or in the U-FC filter cassette).
(Controls Used) (Page)
Set the main switch to “ I” (ON) and adjust
the brightness.
@Main switch
2LED brightness
adjustment knob*
Select the light path (trinocular tube). 5Light path selector knob (P. 22)
Place the specimen on the stage. 6Slide holder (P. 19)
7X-/Y-axis knobs (P. 20)
Engage the 10X objective in the light path. 8Revolving nosepiece
Bring the specimen in focus. 9Coarse/fine adjustment knobs (P. 17)
Adjust the interpupillary distance.
Adjust the diopter.
Adjust the light axis.
aBinocular tube (P. 21)
bDiopter adjustment ring (P. 21)
cCondenser height adjustment knob (P. 24)
dCondenser centering knob (P. 12)
Adjust the aperture iris and field iris diaphragms.
eAperture iris diaphragm ring (P. 12)
fField iris diaphragm ring (P. 12)
Engage the desired objective in the light path
and bring the specimen in focus.
8Revolving nosepiece
Insert the required filters.
gFilter mount (P. 14)
Adjust the brightness. 2LED brightness
adjustment knob*
Start observation.
LED lamp Halogen bulb
3Main switch
|Brightness
adjustment knob
(P. 13)
|Brightness
adjustment knob
(P. 13)
*The LED brightness adjustment knob is defeated when the LIM is set (see page 34).

9
BX43
}Copy the observation procedure pages on separate sheets and post it near your microscope.
9
a5(trinocular tube only)
d
c
e
2
f
3
7
1
4
b8
6
g

10
SIMPLIFIED OBSERVATION PROCEDURE
4
4-1 Basic Operation (Until Observation of Specimen)
This section describes the basic operation of the microscope until the start of observation of a specimen. For the detailed
operating procedure of each control, please read the description page specified below.
Press the main switch of the microscope frame to “ I” (ON).
Rotate the knob to adjust the lamp brightness. (Details: Page 13)
Rotate the knob to lower the stage. (Details: Page 18)
4
3
1
2
Open the clamping lever of the slide holder and place the specimen
slide. (Details: Page 18)
Fig. 3
Fig. 4
1
2
Fig. 5
3
4

11
BX43
Fig. 7
Fig. 6
Fig. 8
5
6
7
1
1
2
Rotate the revolving nosepiece to engage the 10X objective in the
light path.
Rotate the coarse and fine adjustment knobs to bring the specimen
into focus. (Details: Page 17)
Rotate the stage knob to adjust the observation position.
Now you can observe the magnified image of the specimen. To
improve the observation further, read section 4-2, “Microscope
Adjustments” below.
4-2 Microscope Adjustments (How to Improve the Observed Image)
Adjust the microscope as described below to improve the observed image.
1Adjusting the Interpupillary Distance
While looking through the eyepieces, adjust for binocular vision until
the left and right fields of view coincide completely.
}Note your interpupillary distance so that it can be quickly duplicated.
2Adjusting the Left and Right Focusing
(diopter adjustment)
Looking through the eyepiece without the diopter adjustment ring,
rotate the coarse and fine adjustment knobs to bring the specimen
into focus.
Looking through the eyepiece with the diopter adjustment ring, turn
only the diopter adjustment ring to focus on the specimen.
}If the diopter adjustment rings are provided on both the left and
right sides, see “Adjusting the Diopter” on page 21.
The diopter adjustment rings may be provided on either the eye-
pieces or the observation tube.
1
2
5
6
7

12
3Adjusting the Centering
Place the specimen.
Rotate the revolving nosepiece to select the 10X objective.
Rotate the knobs to bring the specimen into focus.
Rotate the knob to raise the condenser to its upper limit.
Rotate the field iris diaphragm ring in the direction of the arrow so that the diaphragm image comes inside the field
of view.
Rotate the knobs to focus on the field iris diaphragm image (A in Fig. 9).
Turn the two condenser centering screws to move the iris diaphragm image to the center of the field of view (B in Fig. 9).
Gradually open the field iris diaphragm until its image is inscribed in the field of view (C in Fig. 9).
Open the field diaphragm slightly until its image circumscribes the field of view slightly (D in Fig. 9).
1
2
3
4
5
6
7
8
9
1
2
}Be careful not to rotate the condenser clamping screw, located behind the centering knobs, by mistake.
}When using a condenser with top lens such as the U-SC3, engage the top lens in the light path before proceeding to
the centering adjustment.
Fig. 9 Movement of Field Iris Diaphragm Image
4Adjusting the Contrast
Set the condenser aperture iris diaphragm ring so that the condenser NA scale position indicates 70% of the NA of the
objective in use. After this, observe the image and fine adjust the aperture iris diaphragm to the desired condition.
The image of the aperture iris diaphragm can be viewed by removing the eyepieces and looking into the observation
tube. Adjust so that the image looks approximately like that shown in the figure above.
A B C D
1
2
Aperture iris
diaphragm image
Objective pupil
70-80%
30-20%
N.A. scale
1
2
3
5
4 6
7
8 9

13
BX43
5USING THE CONTROLS
5-1 Base
Fig. 10
Fig. 11
Fig. 12
1Adjusting the Brightness (Fig. 10)
1. When the LED lamp is used for observation, turn the LED brightness
adjustment knob @ clockwise to make illumination brighter.
2. When the halogen bulb is used for observation, adjust the brightness
adjustment knob 2of the TL4 power supply unit.
2Setting the LIM Brightness (Fig. 12)
}LIM stands for Light Intensity Manager. It is effective when the BX43-5RES
revolving nosepiece with sensor and the U-LHLEDC LED lamp housing
are used.
}The desired brightness can be set per objective. Once the brightness for
the objectives is set, selecting an objective adjusts the LED brightness
automatically provided that the LIM ON-OFF switch @ is set to “ON” (which
is indicated by the lighting of the LIM indicator 2).
1. Press the LIM ON-OFF switch @ to “OFF” (so that the LIM indicator 2is
extinguished).
2. Engage a low-power objective in the light path and bring the specimen
in focus.
3. Rotate the brightness adjustment knob 3for optimum brightness for
observation.
4. Press the LIM setting switch | to enter the current brightness.
5. Set the optimum brightness for the mounted objectives by repeating the
above for each of them.
To modify the LIM setting:
Engage the objective you want to set the new LIM setting in the light path,
set the LIM ON-OFF switch to “OFF,” and perform steps 3 and 4 above.
1
4
2
1
3
2

14
Fig. 14
Fig. 15
Using the Filter Cassette (Figs. 14 to 17)
Loading Filters into Filter Cassette
}The filter cassette accommodates filters with a diameter of 45 mm and
thickness of 2.7 mm or less.
}The filter cassette has two filter levels on the right side and one on the
left side.
}When the filter cassette is used, the ECO mode cannot be used because
the human sensor is hidden by the filter cassette.
1. Move all filter levers to the OUT position except for the one belonging to
the slot into which the filter is to be inserted.
2. Slide lever @ to the IN position. Make sure that it clicks securely into
place. (Fig. 14)
3. Holding the lever in the position shown in Fig. 14, put the filter into the
cassette by inserting it in the direction indicated by the arrow.
4. Insert the other two filters in the same manner.
1
Fig. 13
3Using the Filters (Halogen Bulb Operation Only)
}You can place a filter in the light path with either method.
· Place a filter on the filter mount on the base and engage in the light path.
(Page 14)
· Insert a filter in the U-FC filter cassette, attach it on the filter mount and
engage it in the light path by sliding the filter level. (Page 14)
Mounting a Single Filter (Fig. 13)
A filter with a diameter of 45 mm can be placed on the filter mount on
the base. If you need to place multiple filters, please purchase the U-FC
filter cassette.
Even when a filter cassette is used, a filter with thickness of no
more than 3 mm can still be placed on the filter mount.
Mounting the Filter Cassette
1. Fully loosen the filter cassette clamping screw 2. (Fig. 15)
2. Align the key 3on the bottom surface of the filter cassette with the
positioning slot | on the filter mount, then snap the filter cassette into
place from above.
3. Rotate the filter cassette to align its sides with the base. (Fig. 16)
4. Align the clamping screw 2with the positioning hole 5on the filter
mount, then tighten the screw to fasten the filter cassette.
When the filter cassette is installed, the condenser may hit it
when the stage is lowered. Therefore, exercise caution when
lowering the stage with the filter cassette installed.
4
2
3
5
CAUTION
CAUTION

15
BX43
Fig. 16
Fig. 17
Using the Filter Cassette (Fig. 17)
Usable Filters Applications
45LBD-IF*Color balancing filter
45-ND6, 45-ND25 Neutral density filter
45G-530, 43IF550-W45*Green B&W contrast filters
45O-560 Orange
Up to three of the above filters can be loaded in the filter cassette. Moving
the levers 6on the left and right sides of the cassette to the IN position
brings the corresponding filter into the light path.
*When an IF (interference) type filter is used, flare and ghost may be
observed. These phenomena may be reduced by adding ND filter(s) to
the IF filter.
}The LBD filter is not required when the LED lamp is used.
What is LBD filter?
The color of the sun at the noon of a clear day is called the daylight
color, which looks white to human eyes. On the other hand, the
halogen bulb used as the light source of this microscope does not
emit the daylight-color light. so the LBD filter is engaged in the light
path to convert the color of halogen bulb light into daylight. In general,
engaging the LBD filter makes it possible to observe specimens in
natural color tones.
6

16
Fig. 18
Fig. 19
Fig. 20
5-2 Focusing Block
1Focusing Controls
The focusing block consists of the controls listed in the following table,
which also describes the functions of the controls.
No. Name Function
1Coarse adjustment
knob
Moves the focus position widely.
2Fine adjustment dial Fine adjusts the focus position.
3Fine adjustment
knob
Fine adjusts the focus position.
This knob can be attached to the fine
adjustment dial on either the left or right
side. (When the microscope is shipped,
it is attached to the right-side dial.)
4Pre-focusing lever Sets the upper limit of the stage position
(to prevent contact between the speci-
men and objective).
5Tension adjustment
ring
Adjusts the force required to rotate the
coarse adjustment knob.
2Adjusting the Focus
With both the coarse adjustment knob @ and fine adjustment knob 2,
rotating the knob in the direction of the arrow raises the stage (lets the
specimen approach the objective).
1
2
5
1
3
4
1
2