Optika Italy B-290 Series User manual

Model
B-290 series (B-292 / B-292PLI / B-293 / B-293PLI)
B-290LD series (B-292LD1.50 / B-292LD1 / B-293LD1.50 / B-293LD1)
B-290TB series (B-290TB)
B-290 Series
INSTRUCTION MANUAL
Ver. 5.0 2020

Page 2
1. Warning 3
2. Symbols and conventions 3
3. Safety Information 3
4. Intended use 3
5. Overview 4
5.1 B-292 / B-292PLI / B-293 / B-293PL 4
5.2 B-292LD1.50 - B-292LD1 - B-293LD1.50 - B-293LD1 5
5.3 B-290TB 6
6. Unpacking 7
7. Assembling 7
7.1 B-292 / B-292PLI / B-293 / B-293PLI 7
7.2 B-292LD1 / B-292LD1.50 / B-293LD1 / B-293LD1.50 8
7.3 B-290TB 9
7.4 Assembling the microscope 10
7.4.1 B-292 / B-292PLI / B-293 / B-293PLI 10
7.4.3 B-290TB 12
7.5 Polarizing set (optional) 14
8. Use of the microscope 15
8.1 Switching on the microscope 15
8.2 Light intensity adjustment 15
8.3 Coarse focus tension adjustment 15
8.4 Stage 15
8.5 Adjust the interpupillary distance 16
8.6 Diopter adjustment 16
8.7 Use of oil immersion objective 16
8.8 Condenser centering 17
8.9 Aperture diaphragm 17
8.10 Useofuorescence 18
8.11 Use of the polarizer (optional) 18
9. Microphotography 19
9.1 Cameras with projection lens 19
9.2 Reexcamera 19
10. Use of software and digital head 20
11. Maintenance 21
12. Troubleshooting 22
Equipment disposal 23
Summary

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of
maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you
that this manual contains important information on safety and maintenance, and that it must therefore be made
accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses
that does not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibility
to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
4. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the
subject.

Page 4
5. Overview
FINE FOCUS KNOB
PHOTO TUBE (ONLY FOR
TRINOCULAR HEAD)
INTENSITY ADJUSTMENT DIAL
STAGE
OBJECTIVES
COARSE FOCUS KNOB
CONDENSER
X/Y MOVEMENT
KNOBS
SLIDE HOLDER
CONDENSER
HEIGHT ADJUSTMENT
KNOB
CONDENSER
CENTERING SCREWS
OBSERVATION HEAD
-) BINOCULAR (B-292 / B-292PLI)
-) TRINOCULAR (B-293 / B-293PLI)
EYEPIECES
5.1 B-292 / B-292PLI / B-293 / B-293PL
APERTURE DIAPHRAGM

Page 5
INTENSITY ADJUSTMENT DIAL
COARSE FOCUS KNOB
FINE FOCUS KNOB
CONDENSER
SLIDE HOLDER
CONDENSER
HEIGHT ADJUSTMENT
KNOB
STAGE
OBJECTIVES
X/Y MOVEMENT
KNOBS
CONDENSER
CENTERING SCREWS
EYEPIECES
PHOTO TUBE (ONLY FOR
TRINOCULAR HEAD)
OBSERVATION HEAD
-) BINOCULAR
(B-292LD1 / B-292LD1.50)
-) TRINOCULAR
(B-293LD1 / B-293LD1.50)
FLUORESCENCE
ATTACHMENT
5.2 B-292LD1.50 - B-292LD1 - B-293LD1.50 - B-293LD1
APERTURE DIAPHRAGM

Page 6
INTENSITY ADJUSTMENT DIAL
COARSE FOCUS
KNOB
FINE FOCUS KNOB
STAGE
X/Y MOVEMENT
KNOBS
SLIDE HOLDER OBJECTIVES
DIGITAL HEAD
TABLET
EYEPIECES
CONDENSER
HEIGHT ADJUSTMENT
KNOB
CONDENSER
CENTERING SCREWS
5.3 B-290TB
APERTURE DIAPHRAGM

Page 7
6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or other
residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
7. Assembling
Once opened the box, the microscope parts are the following:
7.1 B-292 / B-292PLI / B-293 / B-293PLI
① Frame
② Observation head
binocular (B-292 / B-292PLI)
trinocular (B-293 / B-293PLI)
③ Photo tube (only B-293 series)
④ Eyepieces
⑤ Objectives (4X / 10X / 40X / 100X)
⑥ Dust cover
⑦ Green lter
⑧ Power supply
⑨ Immersion oil
⑩ Tension adjustment tool
③
④
⑤
⑥
⑦
⑧
①
②
⑨
⑩

Page 8
7.2 B-292LD1 / B-292LD1.50 / B-293LD1 / B-293LD1.50
① Frame
② Observation head
binocular (B-292LD1 / B-292LD1.50)
trinocular (B-293 / B-293PLI)
③ Photo tube (only B-293 series)
④ Eyepieces
⑤ Objectives
10X/20X/40X/50X: B-292LD1.50 - B-293LD1.50
10X/20X/40X/100X(dry): B-292LD1 - B-293LD1
⑥ Dust cover
⑦ Fluorescence attachment
⑧ Power supply
⑨ Tension adjustment tool
③
④
⑤
⑥
⑦
⑧
①
②
⑨

Page 9
7.3 B-290TB
① Frame
② Digital observation head
③ Eyepieces
④ Objectives (4X / 10X / 40X / 100X)
⑤ Dust cover
⑥ Green lter
⑦ Immersion oil
⑧ Power supply
⑨ Tension adjustment tool
⑩ Tablet power supply
⑪ OTG cable
⑫USB cable
⑬ Touch pen
⑭ Tablet + keyboard
②
⑩
⑪
⑫
⑬
⑭
③
④
⑤
⑥
⑦
⑧
①
⑨

Page 10
7.4 Assembling the microscope
7.4.1 B-292 / B-292PLI / B-293 / B-293PLI
1. Remove the dust cap from the microscope frame
and from the bottom of the observation head.
2. Insert the optical head above the stand and tight-
en the screw. (Fig. 1)
• Hold the head with one hand during the
locking in order to avoid that the head falls.
3. Insert both eyepieces into the tubes of the optical
head. (Fig. 2)
4. Insert the power supply jack in the socket placed
at the rear side of the microscope. (Fig. 3)
Only for trinocular head
5. Unscrew the protection cap mounted on the
photo port and screw the photo tube. (Fig. 4)
FFig. 1ig. 1
FFig. 2ig. 2
FFig. 3ig. 3
FFig. 4ig. 4

Page 11
7.4.2 B-292LD1/B-292LD1.50/B-293LD1/B-2932LD1.50
FFig. 5ig. 5
FFig. 6ig. 6
FFig. 8ig. 8
FFig. 7ig. 7
1. Insert the uorescence attachment above the
frame and tighten the locking screw. (Fig. 5)
2. Insert the cable in the socket placed at the rear
side of the microscope. (Fig. 6)
3. Insert the optical head above the stand and tight-
en the screw. (Fig. 7)
• Hold the head with one hand during the lock-
ing in order to avoid that the head falls.
4. Insert both eyepieces into the tubes of the optical
head. (Fig. 8)

Page 12
5. Insert the power supply jack in the socket placed
at the rear side of the microscope. (Fig. 9)
Only for trinocular head
6. Unscrew the protection cap mounted on the pho-
to port and screw the photo tube. (Fig. 10)
7.4.3 B-290TB
1. Remove the dust cap from the microscope frame
and from the bottom of the observation head.
2. Insert the optical head above the stand and tight-
en the screw. (Fig. 11)
• Hold the head with one hand during the lock-
ing in order to avoid that the head falls.
3. Insert both eyepieces into the tubes of the optical
head. (Fig. 12)
4. Insert the power supply jack in the socket placed
at the rear side of the microscope. (Fig. 9)
FFig. 9ig. 9
FFig. 10ig. 10
FFig. 11ig. 11
FFig. 12ig. 12

Page 13
5. Fix the rotating part of the junction using the black
wing-nut ①. (Fig. 13)
6. Then hook the Tablet PC onto the 4 screws of the
junction and pull toward down to rmly lock the
Tablet PC in the holder. (Fig. 14)
• To unlock the Tablet PC proceed with the oppo-
site operation: push toward up and remove it from
the holder.
7. Plug one side of the cable named CAMERA CON-
NECTION (USB + OTG) ② to the digital head
and the other side to the Tablet PC. (Fig. 15).
8. Plug the cable named POWER SUPPLY CON-
NECTION to the Tablet PC for battery recharge.
• The Tablet has been set with the Rotation func-
tion disabled: this prevents any ipping of the Live
View in order to get a continuous and as large as
possible view of your slide also when the Tablet is
removed from the holder.
• To enable this function again: you can activate
the Rotation by swiping the screen on his bot-
tom right side and selecting Settings + Screen.
Anyway, it’s not suggested to activate the func-
tion when the camera is in Live View mode as it
may give troubles when the camera runs at high
resolutions.
FFig. 13ig. 13
FFig. 14ig. 14
①
FFig. 15ig. 15
②

Page 14
7.5 Polarizing set (optional)
1. Place the polarizer on the light exit ①at the base
of the microscope. (Fig. 16)
2. Loosen the head xing knob ② and remove the
head from the microscope frame. (Fig. 17)
3. Insert the analyser into the hole inside the frame
③. (Fig. 18)
4. Put back the head into its original position and
lock the xing knob.
FFig. 18ig. 18
③
FFig. 16ig. 16
①
FFig. 17ig. 17
②

Page 15
8. Use of the microscope
FFig. 7ig. 7
FFig. 19ig. 19
FFig. 20ig. 20
FFig. 21ig. 21
FFig. 22ig. 22
②
①
8.1 Switching on the microscope
1. Operate on the main switch ① placed in the rear
side of the microscope, moving the selector on “I”
(Fig. 19)
• For “LD” models only: there is a three-position
switch on the rear of the stand: position “I” turns
on the transmitted light, position “II” turns on
the uorescence and position “O” turns o󰀨 the
microscope.
8.2 Light intensity adjustment
1. Operate on the light intensity dial to increase or
decrease the illumination intensity. (Fig. 20)
8.3 Coarse focus tension adjustment
• Adjust the tension using the provided tool.
The coarse knob tension is pre-setted in the factory.
1. To modify the tension according to personal’s
needs, rotate the ring using the provided tool
(Fig. 21).
• Clockwise rotation increases the tension.
• If the tension is too loose, the stage could go
lower by itself or the focus easily lost after ne
adjustment. In this case, rotate the knob in order
to increase the tension.
8.4 Stage
Stage accepts standard slides 26 x 76 mm, thickness
1,2 mm with coverslide 0,17mm. (Fig. 22)
1. Open the spring arm of the slide holder ② and
place the slide from the front on the stage.
2. Gently release the spring arm of the slide holder.
• A sudden release of the spring arm could
cause the falling of the slide.

Page 16
8.5 Adjust the interpupillary distance
Hold the right and left parts of the observation head
using both hands and adjust the interpupillary dis-
tance by turning the two parts until one circle of light
can be seen. (Fig. 23)
• The graduation on the interpupillary distance in-
dicator ①, pointed by the spot “.” on the eyepiece
holder, shows the distance between the opera-
tor’s eyes.
The range of the interpupillary distance is 48- 75 mm.
8.6 Diopter adjustment
This operation can be done only on binocular
models.
1. Look into the right eyepiece with your right eye
only, and focus on the specimen.
2. Look into the left eyepiece with your left eye only.
If the image is not sharp, use the dioptric adjust-
ment ring ②to compensate. (Fig. 24)
• The adjustment range is ±5 diopter. The num-
ber indicated on the adjustment ring gradua-
tion should correspond to the operator’s di-
optric correction.
8.7 Use of oil immersion objective
AllmodelsexceptLDseries
1. Focus the specimen with a low power objective.
2. Lower the stage.
3. Put a drop of oil (provided) on the area of the
specimen to be observed. (Fig. 25)
• Make sure that there are no oil bubbles. Air
bubbles in the oil damage the image quality.
• To check for bubbles: remove an eyepiece, fully
open the aperture diaphragm and observe the
objective exit pupil. (The pupil must be circular
and bright).
• To remove the bubbles, gently move the nose-
piece to the right and left to move the immersion
objective a few times and allow the air bubbles to
move.
4. Insert immersion objective.
5. Return the stage to the upper focusing point and
obtain an optimal focus using the ne focus knob.
6. After use, gently remove the oil with a soft pa-
per towel or a lightly moistened optic paper with
a mixture of ethyl ether (70%) and absolute ethyl
alcohol (30%).
• The immersion oil, if not immediately cleaned,
could crystallize creating a glass-like layer.
In this situation the observation of the speci-
menwouldbedi󰀩cult(evennotimpossible)
due to the presence of an additional thickness
on the objective.
FFig. 23ig. 23
FFig. 25ig. 25
①
FFig. 24ig. 24
②

Page 17
8.8 Condenser centering
• The condenser is installed and pre-centered in
the factory.
• To remove the condenser use an Allen wrench
1.5 mm and operate on the xing knob placed on
the right side of the condenser holder.
Should a new centering is needed, operate in this
way:
1. Insert 4x objective in the light path (in case 4x is
not available use the lower magnication avail-
able).
2. Focus the specimen.
3. Close the aperture diaphragm using the ring ①,
moving the ring to the value “4” related to the 4x
objective. (Fig. 26)
4. Raise the condenser to the upper limit using the
height adjustment knob ② placed on the left side
of the condenser holder.
5. Center the condenser using the centering screws
③ until the eld of view is evenly illuminated (in
the eld of view no dark and bright areas must be
noticed).
6. Fully open the diaphragm.
8.9 Aperture diaphragm
• The Numerical Aperture (N.A.) value of the ap-
erture diaphragm a󰀨ects the image contrast. In-
creasing or reducing this value one can vary res-
olution, contrast and depth of focus of the image.
Move the diaphragm ring ① (Fig. 26) on the value
corresponding to the objective in use. In this case
the optimal setting of the condenser is achieved.
• With low contrast specimens set the numerical
aperture to about 70%-80% of the objective’s
N.A. If necessary, remove on eyepiece and, look-
ing into empty sleeve, adjust the condenser’s
diaphragm in order to obtain an image like the
one in Fig. 27.
FFig. 27ig. 27
APERTURE
DIAPHRAGM
FIELD OF VIEW
30-20%
70-80%
FFig. 26ig. 26
①
②
③

Page 18
8.10 Useofuorescence
1. Operate on the main switch placed in the rear
side of the microscope.
• Setting on “I” turns on transmitted light, setting on
“II” turns on uorescence. Setting on “O” turns o󰀨
the microscope. (Fig. 28)
2. Move the lter selector in the “B” position (Fig.
29) to insert the uorescence lter in the light
path. Move the selector in the middle to work with
brighteld transmitted light.
3. Unlike a mercury lamp system, B-290LD LED illu-
mination doesn’t need any power-up time for heat-
ing, and can be used immediately after switching
on. Also, the LED source is pre-aligned in factory
and doesn’t need any alignment operation.
4. Focus on your sample, and adjust the light in-
tensity as needed through the brightness adjust-
ment knob. In order to improve the darkness of
the background (thus improving contrast), it is
strongly suggested to put the put a dark cover on
the light exit at the base of the microscope.
FILTER
NAME EXCITATION FILTER DICHROIC MIRROR EMISSION FILTER APPLICATIONS
B 460 - 490 nm 505 nm 515LP nm
• FITC: fluorescent antibodies
• Achridine orange DNA - RNA
• Auramine
8.11 Use of the polarizer (optional)
1. Remove the specimen from the stage.
2. Looking inside the eyepieces, rotate the polarizer
until the darkest position is achieved.
3. Once the dark is achieved (“extinction” or
“Crossed Nicol” position) it is possible to begin
the observation.
FFig. 28ig. 28
FFig. 29ig. 29

Page 19
9. Microphotography
FFig. 30ig. 30
FFig. 31ig. 31
M1
M1) Screw the Reex adapter to the “T2” ring (provided with the
FFig. 32ig. 32
M2
M3) Properly install the Reex camera’s adapter
on the trinocular port
FFig. 33ig. 33
9.1 Cameras with projection lens
1. Remove dust caps from camera and projection
lens.
2. Screw the projection lens to camera thread. (Fig.
30)
3. Insert the projection lens into the photo tube. (Fig.
31)
9.2 Reexcamera
1. Screw the “T2” ring (not provided) at the end of
the projection lens (M-173), then install every-
thing to the reex camera. (Fig. 32)
2. Insert the projection lens into the photo tube. (Fig.
33)

Page 20
10. Use of software and digital head
The camera inside the digital head is driven by PROVIEW software.
For the instructions about the use of the software, please refer to the specic instruction manual.
Manual can be downloaded using the QR code available on this manual or using the web site.
The PDF version of the manual can be found under the name:
OPTIKA - B-150D - B-190TB - B-290TB Software Setup - Instruction manual - EN IT ES FR DE PT.