Optika Italy B-290 Series User manual

Model
B-290 series (B-292 / B-292PLI / B-293 / B-293PLI)
B-290LD series (B-292LD1.50 / B-292LD1 / B-293LD1.50 / B-293LD1)
B-290TB series (B-290TB)
B-290 Series
INSTRUCTION MANUAL
Ver. 5.0 2020

Page 2
1. Warning 3
2. Symbols and conventions 3
3. Safety Information 3
4. Intended use 3
5. Overview 4
5.1 B-292 / B-292PLI / B-293 / B-293PL 4
5.2 B-292LD1.50 - B-292LD1 - B-293LD1.50 - B-293LD1 5
5.3 B-290TB 6
6. Unpacking 7
7. Assembling 7
7.1 B-292 / B-292PLI / B-293 / B-293PLI 7
7.2 B-292LD1 / B-292LD1.50 / B-293LD1 / B-293LD1.50 8
7.3 B-290TB 9
7.4 Assembling the microscope 10
7.4.1 B-292 / B-292PLI / B-293 / B-293PLI 10
7.4.3 B-290TB 12
7.5 Polarizing set (optional) 14
8. Use of the microscope 15
8.1 Switching on the microscope 15
8.2 Light intensity adjustment 15
8.3 Coarse focus tension adjustment 15
8.4 Stage 15
8.5 Adjust the interpupillary distance 16
8.6 Diopter adjustment 16
8.7 Use of oil immersion objective 16
8.8 Condenser centering 17
8.9 Aperture diaphragm 17
8.10 Useofuorescence 18
8.11 Use of the polarizer (optional) 18
9. Microphotography 19
9.1 Cameras with projection lens 19
9.2 Reexcamera 19
10. Use of software and digital head 20
11. Maintenance 21
12. Troubleshooting 22
Equipment disposal 23
Summary

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of
maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you
that this manual contains important information on safety and maintenance, and that it must therefore be made
accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses
that does not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibility
to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
4. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the
subject.

Page 4
5. Overview
FINE FOCUS KNOB
PHOTO TUBE (ONLY FOR
TRINOCULAR HEAD)
INTENSITY ADJUSTMENT DIAL
STAGE
OBJECTIVES
COARSE FOCUS KNOB
CONDENSER
X/Y MOVEMENT
KNOBS
SLIDE HOLDER
CONDENSER
HEIGHT ADJUSTMENT
KNOB
CONDENSER
CENTERING SCREWS
OBSERVATION HEAD
-) BINOCULAR (B-292 / B-292PLI)
-) TRINOCULAR (B-293 / B-293PLI)
EYEPIECES
5.1 B-292 / B-292PLI / B-293 / B-293PL
APERTURE DIAPHRAGM

Page 5
INTENSITY ADJUSTMENT DIAL
COARSE FOCUS KNOB
FINE FOCUS KNOB
CONDENSER
SLIDE HOLDER
CONDENSER
HEIGHT ADJUSTMENT
KNOB
STAGE
OBJECTIVES
X/Y MOVEMENT
KNOBS
CONDENSER
CENTERING SCREWS
EYEPIECES
PHOTO TUBE (ONLY FOR
TRINOCULAR HEAD)
OBSERVATION HEAD
-) BINOCULAR
(B-292LD1 / B-292LD1.50)
-) TRINOCULAR
(B-293LD1 / B-293LD1.50)
FLUORESCENCE
ATTACHMENT
5.2 B-292LD1.50 - B-292LD1 - B-293LD1.50 - B-293LD1
APERTURE DIAPHRAGM

Page 6
INTENSITY ADJUSTMENT DIAL
COARSE FOCUS
KNOB
FINE FOCUS KNOB
STAGE
X/Y MOVEMENT
KNOBS
SLIDE HOLDER OBJECTIVES
DIGITAL HEAD
TABLET
EYEPIECES
CONDENSER
HEIGHT ADJUSTMENT
KNOB
CONDENSER
CENTERING SCREWS
5.3 B-290TB
APERTURE DIAPHRAGM

Page 7
6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or other
residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
7. Assembling
Once opened the box, the microscope parts are the following:
7.1 B-292 / B-292PLI / B-293 / B-293PLI
① Frame
② Observation head
binocular (B-292 / B-292PLI)
trinocular (B-293 / B-293PLI)
③ Photo tube (only B-293 series)
④ Eyepieces
⑤ Objectives (4X / 10X / 40X / 100X)
⑥ Dust cover
⑦ Green lter
⑧ Power supply
⑨ Immersion oil
⑩ Tension adjustment tool
③
④
⑤
⑥
⑦
⑧
①
②
⑨
⑩

Page 8
7.2 B-292LD1 / B-292LD1.50 / B-293LD1 / B-293LD1.50
① Frame
② Observation head
binocular (B-292LD1 / B-292LD1.50)
trinocular (B-293 / B-293PLI)
③ Photo tube (only B-293 series)
④ Eyepieces
⑤ Objectives
10X/20X/40X/50X: B-292LD1.50 - B-293LD1.50
10X/20X/40X/100X(dry): B-292LD1 - B-293LD1
⑥ Dust cover
⑦ Fluorescence attachment
⑧ Power supply
⑨ Tension adjustment tool
③
④
⑤
⑥
⑦
⑧
①
②
⑨

Page 9
7.3 B-290TB
① Frame
② Digital observation head
③ Eyepieces
④ Objectives (4X / 10X / 40X / 100X)
⑤ Dust cover
⑥ Green lter
⑦ Immersion oil
⑧ Power supply
⑨ Tension adjustment tool
⑩ Tablet power supply
⑪ OTG cable
⑫USB cable
⑬ Touch pen
⑭ Tablet + keyboard
②
⑩
⑪
⑫
⑬
⑭
③
④
⑤
⑥
⑦
⑧
①
⑨

Page 10
7.4 Assembling the microscope
7.4.1 B-292 / B-292PLI / B-293 / B-293PLI
1. Remove the dust cap from the microscope frame
and from the bottom of the observation head.
2. Insert the optical head above the stand and tight-
en the screw. (Fig. 1)
• Hold the head with one hand during the
locking in order to avoid that the head falls.
3. Insert both eyepieces into the tubes of the optical
head. (Fig. 2)
4. Insert the power supply jack in the socket placed
at the rear side of the microscope. (Fig. 3)
Only for trinocular head
5. Unscrew the protection cap mounted on the
photo port and screw the photo tube. (Fig. 4)
FFig. 1ig. 1
FFig. 2ig. 2
FFig. 3ig. 3
FFig. 4ig. 4