Optika Italy B-382PL-ALC User manual

Model
B-382PL-ALC
B-383PL
B-382PLI-ALC
B-383PLI
B-382PH-ALC
B-383PH
B-382PHI-ALC
B-383PHI
B-383FL
B-383LD1
B-383LD2
B-380 Series
INSTRUCTION MANUAL
Ver. 5.0 2019

Page 2
1. Warning 3
2. Symbols and conventions 3
3. Safety Information 3
4. Intended use 3
5. Overview 4
5.1 B-382PL-ALC / B-382PLI-ALC 4
5.2 B-383PL / B-383PLI 5
5.3 B-382PH-ALC / B-382PHI-ALC 6
5.4 B-383PH / B-383PHI 7
5.5 B-383FL 8
5.6 B-383LD1 / B-383LD2 10
6. Unpacking 11
7. Assembling 11
7.1 B-382PL-ALC / B-382PLI-ALC 11
7.2 B-383PL / B-383PLI 12
7.3 B-382PH-ALC / B-382PHI-ALC 12
7.4 B-383PH / B-383PHI 13
7.5 B-383LD1 / B-383LD2 13
7.6 B-383FL 14
7.7 Assembling the microscope 15
7.8 Field Diaphragm (Optional) 16
7.9 Polarizing set (optional) 16
8. Summaryofbrighteldobservationprocedures 18
9. Use of the microscope 19
9.1 Light intensity adjustment 19
9.2 Adjust the interpupillary distance 19
9.3 Diopter adjustment 19
9.4 Coarse focus tension adjustment 19
9.5 Focus lock lever 20
9.6 Stage 20
9.7 Condenser centering 20
9.7.1 Centeringwithoutelddiaphragm 20
9.7.2 Centeringwithelddiaphragm 21
9.8 E󰀨ectsoftheelddiaphragm 21
9.9 Aperture diaphragm 21
9.10 Use of oil immersion objective 22
9.11 Use of ALC system 22
9.12 Use of the polarizer (optional) 22
10. Useofuniversalcondenserforbrighteld/darkeld/phasecontrast 23
10.1 Brighteldobservation(BF) 23
10.2 Darkeldobservation(DF) 23
10.3 Phase contrast observation (PH) 24
10.4 Useofthegreenlter 25
11. Microphotography 25
11.1 Installing the C-mount adapter 25
11.2 Useofreexcameras 25
12. Useinuorescence 26
12.1 Assembling procedure (all models) 26
12.2 HBO bulb assembling (B-383FL) 26
12.3 Centering the HBO bulb (B-383FL) 28
12.4 Use of the microscope (B-383FL) 30
12.5 Use of the microscope (B-383LD1 / LD2) 30
12.6 Use of the shutter 30
12.7 Useofthelightexcludingplate 31
13. Fluorescence observation procedures (B-383FL) 32
14. Fluorescence observation procedures (B-383LD1/LD2) 32
15. Simultaneous observation Phase Contrast + Fluorescence (B-383FL) 33
16. Maintenance 33
17. Troubleshooting 34
Equipment disposal 36
Table of Contents

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of
maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you
that this manual contains important information on safety and maintenance, and that it must therefore be made
accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses
that does not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibility
to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
4. Intended use
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.

Page 4
5. Overview
EYEPIECES
PHOTO/TV PORT
(NOT USABLE WITH ALC
MODELS)
OBSERVATION
HEAD
DIOPTER
ADJUSTMENT
RING
FOCUS LOCK
LEVER
MAIN SWITCH /
LIGHT INTENSITY
DIAL
CONDENSER
STAGE
CONDENSER
HEIGHT
ADJUSTMENT
KNOB
NOSEPIECE
OBJECTIVES
ALC SYSTEM
CONNECTION
CABLE
X-Y
MOVEMENT
KNOBS
CONDENSER
CENTERING
SCREWS
FINE FOCUS KNOB
COARSE FOCUS
KNOB
ALC SYSTEM
ON /OFF BUTTON
SLIDE HOLDER
TENSION
ADJUSTMENT
RING
5.1 B-382PL-ALC / B-382PLI-ALC

Page 5
5.2 B-383PL / B-383PLI
EYEPIECES
PHOTO/TV PORT
OBSERVATION
HEAD
FOCUS LOCK
LEVER
MAIN SWITCH /
LIGHT INTENSITY
DIAL
CONDENSER
STAGE
CONDENSER
HEIGHT
ADJUSTMENT
KNOB
NOSEPIECE
OBJECTIVES
X-Y
MOVEMENT
KNOBS
CONDENSER
CENTERING
SCREWS
FINE FOCUS KNOB
COARSE FOCUS
KNOB
SLIDE HOLDER
DIOPTER
ADJUSTMENT
RING
TENSION
ADJUSTMENT
RING

Page 6
5.3 B-382PH-ALC / B-382PHI-ALC
EYEPIECES
PHOTO/TV PORT
(NOT USABLE WITH ALC
MODELS)
OBSERVATION
HEAD
DIOPTER
ADJUSTMENT
RING
FOCUS LOCK
LEVER
MAIN SWITCH /
LIGHT INTENSITY
DIAL
CONDENSER
STAGE
NOSEPIECE
OBJECTIVES
ALC SYSTEM
CONNECTION
CABLE
X-Y
MOVEMENT
KNOBS
CONDENSER
CENTERING
SCREWS
ALC SYSTEM
ON /OFF BUTTON
SLIDE HOLDER
TENSION
ADJUSTMENT
RING
COARSE FOCUS
KNOB
FINE FOCUS KNOB
CONDENSER
HEIGHT
ADJUSTMENT
KNOB

Page 7
5.4 B-383PH / B-383PHI
EYEPIECES
PHOTO/TV PORT
OBSERVATION
HEAD
FOCUS LOCK
LEVER
MAIN SWITCH /
LIGHT INTENSITY
DIAL
CONDENSER
STAGE
CONDENSER
HEIGHT
ADJUSTMENT
KNOB
NOSEPIECE
OBJECTIVES
X-Y
MOVEMENT
KNOBS
CONDENSER
CENTERING
SCREWS
SLIDE HOLDER
DIOPTER
ADJUSTMENT
RING
TENSION
ADJUSTMENT
RING
COARSE FOCUS
KNOB
FINE FOCUS KNOB

Page 8
5.5 B-383FL
OBSERVATION
HEAD
DIOPTER
ADJUSTMENT
RING
FOCUS LOCK
LEVER
MAIN SWITCH /
LIGHT INTENSITY
DIAL
STAGE
CONDENSER
HEIGHT
ADJUSTMENT
KNOB
NOSEPIECE
OBJECTIVES
SLIDE HOLDER
EYEPIECES
HBO FLUORESCENCE
EPI-ILLUMINATOR
COARSE FOCUS
KNOB
FINE FOCUS KNOB
PHOTO/TV PORT

Page 9
CONDENSER
LAMP
HOUSING
X-Y
MOVEMENT
KNOBS
CONDENSER
CENTERING
SCREWS
TENSION
ADJUSTMENT
RING
HBO FLUORESCENCE
POWER SUPPLY
FLUORESCENCE
FILTER SLIDER
B-383FL (OPPOSITE SIDE)

Page 10
5.6 B-383LD1 / B-383LD2
FOCUS LOCK
LEVER
LIGHT INTENSITY
DIAL
STAGE
CONDENSER
HEIGHT
ADJUSTMENT
KNOB
NOSEPIECE
OBJECTIVES
SLIDE HOLDER
LED FLUORESCENCE
EPI-ILLUMINATOR
OBSERVATION
HEAD
DIOPTER
ADJUSTMENT
RING
EYEPIECES
PHOTO/TV PORT
X-Y
MOVEMENT
KNOBS
CONDENSER
CONDENSER
CENTERING
SCREWS
FLUORESCENCE
FILTER SLIDER
FLUORESCENCE
LED
TENSION
ADJUSTMENT
RING
FINE FOCUS KNOB
COARSE FOCUS
KNOB

Page 11
6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge
of the container and lift the top half of the container. Take some care to avoid that the opti-
cal items (objectives and eyepieces) fall out and get damaged. Using both hands (one around the
arm and one around the base), lift the microscope from the container and put it on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or
other residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
7. Assembling
Once opened the microscope box, the microscope parts are the following:
7.1 B-382PL-ALC / B-382PLI-ALC
① Frame
② Objectives
③ ALC observation head
④ Eyepieces
⑤ Tension adjustment tool
⑥ Dust cover
⑦ Power supply
⑧ Immersion oil
①
③
④
⑤
⑥⑦
⑧
②

Page 12
7.2 B-383PL / B-383PLI
7.3 B-382PH-ALC / B-382PHI-ALC
① Frame
② Objectives
③ Trinocular observation head
④ Eyepieces
⑤ Tension adjustment tool
⑥ Dust cover
⑦ Power supply
⑧ Immersion oil
①
③
④
⑤
⑥⑦
⑧
②
① Frame
② Objectives
③ ALC observation head
④ Eyepieces
⑤ Tension adjustment tool
⑥ Dust cover
⑦ Power supply
⑧ Immersion oil
⑨ Green lter + lter holder
⑩ Centering telescope
①
③
④
⑤
⑥⑦
⑧
②
⑨
⑩

Page 13
7.4 B-383PH / B-383PHI
7.5 B-383LD1 / B-383LD2
① Frame
② Objectives
③ Trinocular observation head
④ Eyepieces
⑤ Tension adjustment tool
⑥ Dust cover
⑦ Power supply
⑧ Immersion oil
⑨ Green lter + lter holder
⑩ Centering telescope
①
③
④
⑤
⑥⑦
⑧
②
⑨
⑩
① Frame
② Objectives
③ Trinocular observation head
④ LED uorescence illuminator
⑤ Eyepieces
⑥ Dust cover
⑦ Immersion oil
⑧ Tension adjustment tool
⑨ Power supply
⑩ Light excluding plate
①
③
④⑤
⑥
⑦⑧②⑨
⑩

Page 14
7.6 B-383FL
① Frame
② Objectives
③ Trinocular observation head
④ HBO uorescence illuminator
⑤ Fluorescence power supply + power cord
⑥ Eyepieces
⑦ Immersion oil
⑧ Tension adjustment tool
⑨ Dust cover
⑩ Power supply
⑪ Light excluding plate
⑫ HBO mercury bulb
①
③
④
⑤⑥
⑦
⑧
②
⑨
⑩
⑫⑪

Page 15
Fig. 1
7.7 Assembling the microscope
1. Insert the optical head above the stand and tighten
the screw with the provided allen wrench. (Fig.1)
ALC models only
2. Connect the ALC cable to the socket on the back
of the frame. (Fig. 2)
3. Insert eyepieces into the empty tubes of the opti-
cal head. (Fig. 3)
4. Screw each objective into the thread of
the nosepiece, clockwise with increasing
magnication. (Fig. 4)
Fig. 2
Fig. 3
Fig. 4

Page 16
5. Insert the power supply jack in the socket placed
in the rear side of the frame. (Fig. 5)
7.8 Field Diaphragm (Optional)
1. Unscrew the lens ath the base of the microsco-
pe. (Fig. 6)
• It may take a little bit of force to unscrew the
lens.
2. Fully screw the eld diaphragm (M-156).
3. System is ready for the use.
7.9 Polarizing set (optional)
1. Place the polarizer on the light exit ①at the base
of the microscope. (Fig. 7)
2. Loosen the head xing knob ② and remove the
head from the microscope frame. (Fig. 8)
Fig. 6
Fig. 7
①
Fig. 8
②
Fig. 5

Page 17
3. Insert the analyzer into the hole inside the frame
③. (Fig. 9)
4. Put back the head into its original position and
lock the xing knob.
• The use of the polarization set, although
possible for models B-383FL, B-383LD1
and B-383LD2, is not recommended. The
presence of the analyzer within the optical
path,duringtheuseofuorescence,causes
asignicantreductionintheamountoflight
projectedonthesample,resultingindi󰀩culty
of observation. Fig. 9
③

Page 18
8. Summaryofbrighteldobservationprocedures
Main switch /
brightness adjustment knob
(Used commands ) (Chapter)
Slide holder
Nosepiece
Observation head
Diopter adjustment ring
Condenser centering
Aperture diaphragm
Field diaphragm
Nosepiece
Main switch /
brightness adjustment knob
Corse and fine focus knobs
Corse and fine focus knobs
Set the main swith to “ON” and adjust light intensity
Place a slide on the stage
Insert 10x objective into the light path
Focus the specimen
Begin observation
Adjust aperture and field diaphragms
Insert in the light path the desired objective and focus the
specimen
Adjust interpupillary distance
Adjust diopter
Center optical axis
Adjust brightness
9.1
9.6
5
5
5
5
9.2
9.3
9.7
9.9
9.7
9.1
X/Y movement knobs 5

Page 19
9. Use of the microscope
9.1 Light intensity adjustment
Operate on the light intensity dial ① to turn ON/OFF
the microscope and to increase or decrease the illu-
mination intensity. (Fig. 10)
• Only for B-383LD1 / B-383LD2: the switch lo-
cated at the back of the microscope operates
to turn on the transmitted light (position “I”)
or the reected light (position “II”). Turn on
the microscope for transmitted light by tur-
ning the switch to “I”.
9.2 Adjust the interpupillary distance
Hold the right and left parts of the observation head
using both hands and adjust the interpupillary distan-
ce by turning the two parts until one circle of light can
be seen. (Fig. 11)
9.3 Diopter adjustment
1. Look into the right eyepiece with your right eye
only, and focus on the specimen.
2. Look into the left eyepiece with your left eye
only. If the image is not sharp, use the dioptric
adjustment ring ② to compensate. (Fig. 12)
• Highpoint eyepieces allow the use also to glass
wearers.
• NOTE: For optimal parfocality, we recommend
using your glasses during normal microscope
use.
9.4 Coarse focus tension adjustment
To modify the tension according to personal’s needs,
rotate the ring ③ using the provided tool (Fig. 13).
Clockwise rotation increases the tension.
• NOTE: If the tension is too loose, the stage could
go lower by itself or the focus easily lost after ne
adjustment. In this case, rotate the knob in order
to increase the tension.
Fig. 10
①
Fig. 11
Fig. 12
②
Fig. 13
③

Page 20
9.5 Focus lock lever
The upper limit knob has two functions: prevent the
contact between slide and objective and acts as
focus memory.
1. After focussing the specimen, rotate the knob ①
and lock it (Fig. 14). In this way the focus upper
limit is set.
2. lower the stage with coarse focus knob and
replace the specimen.
3. Raise again the stage up to the upper limit:
specimen will be in approximate focus and will
need a ne adjustment to get the proper focus.
Fine focus movement is not a󰀨ected by the
coarse focus lock.
• To unlock, move the knob in the opposite
direction to the one used for the lock.
9.6 Stage
Stage accepts standard slides 26 x 76 mm, thickness
1,2 mm with coverlside 0,17mm. (Fig. 15)
It is possible to place two slides side by side on the
stage.
1. Open the spring arm of the slide holder ② and
place frontally the slides on the stage.
2. Gently release the spring arm of the slide holder.
• A sudden release of the the spring arm could
cause the falling of the slide.
9.7 Condenser centering
9.7.1 Centeringwithoutelddiaphragm
The condenser is installed and pre-centered in the
factory.
To remove the condenser use an Allen wrench 1.5
mm and operate on the xing knob placed on the
right side of the condenser holder.
Should a new centering is needed, operate in this
way:
1. Insert 4x objective in the light path (in case 4x is
not available use the lower magnication availa-
ble).
2. Focus the specimen.
3. Close the aperture diaphragm using the ring ③,
moving the ring to the value “4” related to the 4x
objective. (Fig. 16)
4. Raise the condenser to the upper limit using the
height adjustment knob ④ placed on the left side
of the condenser holder.
5. Center the condenser using the centering screws
⑤ until the eld of view is evenly illuminated (in
the eld of view no dark and bright areas must be
noticed).
6. Fully open the diaphragm.
Fig. 14
①
Fig. 15
②
Fig. 16
⑤
③
④