Optika Italy B-382PL-ALC User manual

Model
B-382PL-ALC
B-383PL
B-382PLI-ALC
B-383PLI
B-382PH-ALC
B-383PH
B-382PHI-ALC
B-383PHI
B-383FL
B-383LD1
B-383LD2
B-380 Series
INSTRUCTION MANUAL
Ver. 5.0 2019

Page 2
1. Warning 3
2. Symbols and conventions 3
3. Safety Information 3
4. Intended use 3
5. Overview 4
5.1 B-382PL-ALC / B-382PLI-ALC 4
5.2 B-383PL / B-383PLI 5
5.3 B-382PH-ALC / B-382PHI-ALC 6
5.4 B-383PH / B-383PHI 7
5.5 B-383FL 8
5.6 B-383LD1 / B-383LD2 10
6. Unpacking 11
7. Assembling 11
7.1 B-382PL-ALC / B-382PLI-ALC 11
7.2 B-383PL / B-383PLI 12
7.3 B-382PH-ALC / B-382PHI-ALC 12
7.4 B-383PH / B-383PHI 13
7.5 B-383LD1 / B-383LD2 13
7.6 B-383FL 14
7.7 Assembling the microscope 15
7.8 Field Diaphragm (Optional) 16
7.9 Polarizing set (optional) 16
8. Summaryofbrighteldobservationprocedures 18
9. Use of the microscope 19
9.1 Light intensity adjustment 19
9.2 Adjust the interpupillary distance 19
9.3 Diopter adjustment 19
9.4 Coarse focus tension adjustment 19
9.5 Focus lock lever 20
9.6 Stage 20
9.7 Condenser centering 20
9.7.1 Centeringwithoutelddiaphragm 20
9.7.2 Centeringwithelddiaphragm 21
9.8 E󰀨ectsoftheelddiaphragm 21
9.9 Aperture diaphragm 21
9.10 Use of oil immersion objective 22
9.11 Use of ALC system 22
9.12 Use of the polarizer (optional) 22
10. Useofuniversalcondenserforbrighteld/darkeld/phasecontrast 23
10.1 Brighteldobservation(BF) 23
10.2 Darkeldobservation(DF) 23
10.3 Phase contrast observation (PH) 24
10.4 Useofthegreenlter 25
11. Microphotography 25
11.1 Installing the C-mount adapter 25
11.2 Useofreexcameras 25
12. Useinuorescence 26
12.1 Assembling procedure (all models) 26
12.2 HBO bulb assembling (B-383FL) 26
12.3 Centering the HBO bulb (B-383FL) 28
12.4 Use of the microscope (B-383FL) 30
12.5 Use of the microscope (B-383LD1 / LD2) 30
12.6 Use of the shutter 30
12.7 Useofthelightexcludingplate 31
13. Fluorescence observation procedures (B-383FL) 32
14. Fluorescence observation procedures (B-383LD1/LD2) 32
15. Simultaneous observation Phase Contrast + Fluorescence (B-383FL) 33
16. Maintenance 33
17. Troubleshooting 34
Equipment disposal 36
Table of Contents

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of
maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you
that this manual contains important information on safety and maintenance, and that it must therefore be made
accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses
that does not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibility
to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
4. Intended use
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.

Page 4
5. Overview
EYEPIECES
PHOTO/TV PORT
(NOT USABLE WITH ALC
MODELS)
OBSERVATION
HEAD
DIOPTER
ADJUSTMENT
RING
FOCUS LOCK
LEVER
MAIN SWITCH /
LIGHT INTENSITY
DIAL
CONDENSER
STAGE
CONDENSER
HEIGHT
ADJUSTMENT
KNOB
NOSEPIECE
OBJECTIVES
ALC SYSTEM
CONNECTION
CABLE
X-Y
MOVEMENT
KNOBS
CONDENSER
CENTERING
SCREWS
FINE FOCUS KNOB
COARSE FOCUS
KNOB
ALC SYSTEM
ON /OFF BUTTON
SLIDE HOLDER
TENSION
ADJUSTMENT
RING
5.1 B-382PL-ALC / B-382PLI-ALC

Page 5
5.2 B-383PL / B-383PLI
EYEPIECES
PHOTO/TV PORT
OBSERVATION
HEAD
FOCUS LOCK
LEVER
MAIN SWITCH /
LIGHT INTENSITY
DIAL
CONDENSER
STAGE
CONDENSER
HEIGHT
ADJUSTMENT
KNOB
NOSEPIECE
OBJECTIVES
X-Y
MOVEMENT
KNOBS
CONDENSER
CENTERING
SCREWS
FINE FOCUS KNOB
COARSE FOCUS
KNOB
SLIDE HOLDER
DIOPTER
ADJUSTMENT
RING
TENSION
ADJUSTMENT
RING

Page 6
5.3 B-382PH-ALC / B-382PHI-ALC
EYEPIECES
PHOTO/TV PORT
(NOT USABLE WITH ALC
MODELS)
OBSERVATION
HEAD
DIOPTER
ADJUSTMENT
RING
FOCUS LOCK
LEVER
MAIN SWITCH /
LIGHT INTENSITY
DIAL
CONDENSER
STAGE
NOSEPIECE
OBJECTIVES
ALC SYSTEM
CONNECTION
CABLE
X-Y
MOVEMENT
KNOBS
CONDENSER
CENTERING
SCREWS
ALC SYSTEM
ON /OFF BUTTON
SLIDE HOLDER
TENSION
ADJUSTMENT
RING
COARSE FOCUS
KNOB
FINE FOCUS KNOB
CONDENSER
HEIGHT
ADJUSTMENT
KNOB

Page 7
5.4 B-383PH / B-383PHI
EYEPIECES
PHOTO/TV PORT
OBSERVATION
HEAD
FOCUS LOCK
LEVER
MAIN SWITCH /
LIGHT INTENSITY
DIAL
CONDENSER
STAGE
CONDENSER
HEIGHT
ADJUSTMENT
KNOB
NOSEPIECE
OBJECTIVES
X-Y
MOVEMENT
KNOBS
CONDENSER
CENTERING
SCREWS
SLIDE HOLDER
DIOPTER
ADJUSTMENT
RING
TENSION
ADJUSTMENT
RING
COARSE FOCUS
KNOB
FINE FOCUS KNOB

Page 8
5.5 B-383FL
OBSERVATION
HEAD
DIOPTER
ADJUSTMENT
RING
FOCUS LOCK
LEVER
MAIN SWITCH /
LIGHT INTENSITY
DIAL
STAGE
CONDENSER
HEIGHT
ADJUSTMENT
KNOB
NOSEPIECE
OBJECTIVES
SLIDE HOLDER
EYEPIECES
HBO FLUORESCENCE
EPI-ILLUMINATOR
COARSE FOCUS
KNOB
FINE FOCUS KNOB
PHOTO/TV PORT

Page 9
CONDENSER
LAMP
HOUSING
X-Y
MOVEMENT
KNOBS
CONDENSER
CENTERING
SCREWS
TENSION
ADJUSTMENT
RING
HBO FLUORESCENCE
POWER SUPPLY
FLUORESCENCE
FILTER SLIDER
B-383FL (OPPOSITE SIDE)

Page 10
5.6 B-383LD1 / B-383LD2
FOCUS LOCK
LEVER
LIGHT INTENSITY
DIAL
STAGE
CONDENSER
HEIGHT
ADJUSTMENT
KNOB
NOSEPIECE
OBJECTIVES
SLIDE HOLDER
LED FLUORESCENCE
EPI-ILLUMINATOR
OBSERVATION
HEAD
DIOPTER
ADJUSTMENT
RING
EYEPIECES
PHOTO/TV PORT
X-Y
MOVEMENT
KNOBS
CONDENSER
CONDENSER
CENTERING
SCREWS
FLUORESCENCE
FILTER SLIDER
FLUORESCENCE
LED
TENSION
ADJUSTMENT
RING
FINE FOCUS KNOB
COARSE FOCUS
KNOB