Optika Italy B-510 Series User manual

Ver. 1.3 2022
INSTRUCTION MANUAL
B-510 Series
Model
B-510MET
B-510METR

Page 2
Table of Contents
1. Warning 3
2. Safety Information 3
3. Package content 4
3.1 B-510MET 4
3.2 B-510MET-R 5
4. Unpacking 6
5. Intended use 6
6. Symbols and conventions 6
7. Instrument description 7
7.1 B-510MET 7
7.2 B-510MET-R 9
8. Assembling 11
9. Summary of reflected light observation procedures 15
10. Use of the microscope 16
10.1 Light intensity adjustment 16
10.2 Coarse focus tension adjustment 16
10.3 Coarse upper limit lever 16
10.4 Stage 16
10.4.1 B-510MET 16
10.4.2 B-510MET-R 17
10.5 Diopter adjustment 17
10.6 Adjusting the interpupillary distance 17
10.7 Use of eyeshields 17
10.8 Centering of reected light diaphragms 18
10.8.1 Field diaphragm (FS) 18
10.8.2 E󰀨ects of the eld diaphragm 18
10.8.3 Aperture diaphragm (AS) 19
10.9 Centering the condenser (B-510MET-R) 19
10.10 Aperture diaphragm 19
10.11 Use of lters 20
10.12 Oblique illumination 20
10.13 Use of polarized light 21
11. Microfotography 22
11.1 Use of C-mount cameras 22
11.2 Use of reex cameras 22
12. Maintenance 23
13. Troubleshooting 24
Equipment disposal 26

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of maintenance. It
is built to high optical and mechanical standards and to withstand daily use. We remind you that this manual contains
important information on safety and maintenance, and that it must therefore be made accessible to the instrument users.
We decline any responsibility deriving from incorrect instrument use uses that does not comply with this manual.
2. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the operation voltage
of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety regulations of the region. The
equipment has acquired the CE safety label. However, users have full responsibility to use this equipment safely. Please
follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.

Page 4
3. Package content
3.1 B-510MET
① Microscopeframe
② Eyepieces
③ Objectives
④ Observationhead
⑤ ReectedlightIlluminator
⑥ Allenwrench
⑦ Polarizer
⑧ Analyzer
⑨ Dustcover
⑩ Connectionplate
⑪ Colorlters
⑫ Powersupply
⑬ Tensionadjustmenttool
③
④
⑤
⑥
⑦
⑧
⑨
①
②
⑪
⑩
⑫⑬

Page 5
3.2 B-510MET-R
① Microscopeframe
② Eyepieces
③ Objectives
④ Observationhead
⑤ Reectedlightilluminator
⑥ Allenwrenches
⑦ Polarizer
⑧ Analyzer
⑨ Dustcover
⑩ Colorlters
⑪ Connectionplate
⑫ Condenser
⑬ Powersupply
⑭ Tensionadjustmenttool
①
②
③
④
⑤
⑥⑦
⑧
⑨
⑩
⑪
⑬
⑫
⑭

Page 6
4. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container and lift the
top half of the container. Take some care to avoid that the optical items (objectives and eyepieces) fall out and get dam-
aged. Using both hands (one around the arm and one around the base), lift the microscope from the container and put it
on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or other
residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
5. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the subject.
6. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.

Page 7
PHOTO/TV PORT
DIOPTER
ADJUSTMENT RING
NOSEPIECE
COARSE
UPPER
LIMIT KNOB
OBJECTIVES
MAIN SWITCH /
BRIGHTNESS ADJUSTMENT
KNOB
OBSERVATION
HEAD
REFLECTED LIGHT
ILLUMINATOR
OBLIQUE
ILLUMINATION
SLIDER
FILTER SLIDER
7. Instrument description
7.1 B-510MET

Page 8
EYEPIECES
STAGE
BINOCULAR
TUBE
X/Y MOVEMENT
KNOBS
TENSION ADJUSTMENT RING
SLIDE HOLDER
FINE FOCUS
KNOB
COARSE FOCUS
KNOB
ANALYZER
POLARIZER
FIELD DIAPHRAGM
(FS)
APERTURE
DIAPHRAGM (AS)
Opposite side

Page 9
PHOTO/TV PORT
DIOPTER
ADJUSTMENT RING
NOSEPIECE
COARSE
UPPER
LIMIT KNOB
CONDENSER
CENTERING SCREWS
OBJECTIVES
APERTURE DIAPHRAGM
(TRANSMITTED LIGHT)
FIELD DIAPHRAGM
(TRANSMITTED LIGHT)
BRIGHTNESS
ADJUSTMENT
KNOB
OBSERVATION
HEAD
CONDENSER HEIGHT
ADJUSTMENT KNOB
OBLIQUE
ILLUMINATION
SLIDER
REFLECTED LIGHT
ILLUMINATOR
TRANSMITTED/REFLECTED
LIGHT SELECTOR
FILTER SLIDER
7.2 B-510MET-R

Page 10
EYEPIECES
STAGE
CONDENSER
BINOCULAR
TUBE
X/Y MOVEMENT
KNOBS
FINE FOCUS
KNOB
COARSE FOCUS
KNOB
TENSION ADJUSTMENT RING
SLIDE HOLDER
FIELD DIAPHRAGM (FS)
(REFLECTED LIGHT)
APERTURE DIAPHRAGM (AS)
(REFLECTED LIGHT)
ANALYZER
POLARIZER
TRANSMITTED/REFLECTED
LIGHT MAIN SWITCH
Opposite side

Page 11
FFig. 1ig. 1
FFig. 2ig. 2
FFig. 4ig. 4
FFig. 3ig. 3
①
②
③
④
⑤
8. Assembling
1. Insert the reected light illuminator ① on the frame and lock
the locking knob ② with the provided Allen wrench. (Fig. 1)
2. Remove the cover ③ on the back side of the illuminator.
(Fig. 2)
3. Insert the LED housing ④ and lock the locking screws ⑤.
(Fig. 3-4)

Page 12
4. Connect the illuminator cable ⑥to the connector ⑦placed
on the back side of the frame. (Fig. 5)
FFig. 5ig. 5
FFig. 6ig. 6
FFig. 8ig. 8
FFig. 7ig. 7
⑥
⑦
⑧
5. Insert the optical head above the epi-illuminator and tighten
the locking screw ⑧ with the provided Allen wrench. (Fig. 6)
• Hold the head with one hand during the locking in order
to avoid that the head falls.
(Only B-510MET-R)
• Condenser is pre-installed in the factory. To remove the
condenser use an Allen wrench 1,5 mm diam and operate on
the locking screw placed on the right side of the condenser
holder.
6. Insert both eyepieces into the tubes of the optical head. (Fig.
7)
7. Screw each objective into the thread of the nosepiece,
clockwise with increasing magnication. (Fig. 8)

Page 13
FFig. 9ig. 9
FFig. 10ig. 10
FFig. 12ig. 12
FFig. 11ig. 11
8. Insert the polarizer “PO” with the inscription facing the user
(before removing the empty slider). (Fig. 9)
9. Insert analyzer “AN” with the inscription facing up (rst removing
the white covering plates on the illuminator). (Fig.10-11)
• It is possible to combine the analyzer and the polarizer
together (using the connection plate) so that the insertion of
the two lters is simultaneous.
10. Screw the plate xing screws to the holes on the end of the
lters. (Fig. 12)

Page 14
11. Insert the power supply jack in the connector placed on the
back side of the frame. (Fig. 13)
FFig. 13ig. 13

Page 15
9. Summary of reflected light observation procedures
(Used commands) (Chapter)
Put on “ON” the main switch and adjust brightness
Place a specimen on the stage
Insert 10x objective into the light path
Focus the specimen
Begin observation
Adjust aperture and field diaphragms
Insert the desired objective into the light path and focus the
specimen
Adjust interpupillary distance
Adjust diopter
Adjust brightness
Remove polarizer, analyzer and filters
from the light path
Insert color filters
Move on “R” the light path selector
(B-510METR)
Main switch / brightness adjustment knob 10
X/Y movement knobs 7
Slide holder 10
Nosepiece 7
Coarse and fine focus knobs 7
Observation head 10
Diopter adjustment ring 10
Aperture diaphragm 10
Field diaphragm 10
Nosepiece 7
Coarse and fine focus knobs 7
Main switch / brightness adjustment knob 10
Transmitted/Reected Light Selector 7
Filter slider 10

Page 16
10. Use of the microscope
FFig. 14ig. 14
FFig. 15ig. 15
FFig. 17ig. 17
FFig. 16ig. 16
10.1 Light intensity adjustment
Operate on the light intensity adjustment knob to turn ON / OFF
the microscope and to increase / decrease the illumination
voltage ①. (Fig. 14)
• For B-510MET-R only. There is a three-position switch on
the rear of the stand: position “I” turns on the transmitted
light, position “II” turns on the reected light and position “O”
turns o󰀨 the microscope.
①
②
③
④
10.2 Coarse focus tension adjustment
• Adjust the tension using the provided tool.
The tension of the coarse focusing knob is factory preset.
1. To modify the tension according to personal’s needs, rotate
the ring ② . (Fig. 15)
• Clockwise rotation increases the tension.
• If the tension is too loose, the stage could go lower by itself or
the focus easily lost after ne adjustment. In this case, rotate
the knob in order to increase the tension .
10.3 Coarse upper limit lever
The upper limit knob has two functions: prevent the contact
between slide and objective and acts as “focus memory”.
1. After focusing the specimen, pull the lever ③ toward the
front of the microscope and lock it. (Fig. 16)
• In this way the focus upper limit is set.
2. Now one can lower the stage with coarse focus knob, replace
the specimen and raise again the stage up to the upper limit:
specimen will be in approximate focus and will need a ne
adjustment to get the proper focus.
• Fine focus movement is not a󰀨ected by the coarse
focus lock.
• To unlock, move the lever in the opposite direction to the
one used for the locking.
10.4 Stage
10.4.1 B-510MET
Stage accepts metallurgical samples with thickness max 35
mm, placed on a standard support 26 x 76 mm.
• Open the spring arm of the slide holder ④ and place
frontally the slides on the stage. (Fig. 17)
• Gently release the spring arm of the slide holder.
• A sudden release of the spring arm could cause the
falling of the slide.

Page 17
10.4.2 B-510MET-R
Stage accepts metallurgical samples with thickness max 35
mm, placed on a standard support 26 x 76 mm, or standard
slides 26x76 mm.(Fig. 18)
• Open the spring arm of the slide holder ④ and place
frontally the slides on the stage. (Fig. 18)
• Gently release the spring arm of the slide holder.
• A sudden release of the spring arm could cause the
falling of the slide.
FFig. 18ig. 18
FFig. 19ig. 19
FFig. 21ig. 21
FFig. 20ig. 20
①
②
④
10.5 Diopter adjustment
1. Look into the right eyepiece with your right eye only, and
focus on the specimen.
2. Look into the left eyepiece with your left eye only. If the
image is not sharp, use the diopter adjustment ring ①to
compensate. (Fig. 19)
• The adjustment range is ±5 diopter. The number indicated
on the adjustment ring graduation should correspond to
the operator’s diopter correction.
10.6 Adjusting the interpupillary distance
Observing with both eyes, hold the two eyepiece prism assem-
blies. Rotate them around their common axis until the elds of
view coincide.
• The graduation on the interpupillary distance indicator
②, pointed by the spot “.” on the eyepiece holder, shows
the distance between the operator’s eyes. (Fig. 34)
The range of the interpupillary distance is 48-75 mm.
10.7 Use of eyeshields
• Use with eyeglasses
Fold rubber eyeshields with both hands. Folded eyeshields avoid
scratching the lenses of eyeglasses. (Fig. 21)

Page 18
• Use without eyeglasses
Raise eye shields and observe at the microscope placing eyes
to the shields, avoiding external light to disturb the observation.
(Fig. 22)
FFig. 22ig. 22
FFig. 23ig. 23
FFig. 25ig. 25
FFig. 24ig. 24
①
②
③
10.8 Centering of reected light diaphragms
10.8.1 Field diaphragm (FS)
1. Move the selector ① on the reected light illuminator in the
position corresponding to letter “R” (only B-510MET-R). (Fig.
23)
2. Place the specimen on the stage, insert 10x objective into
the light path and focus.
3. Completely pull out the eld diaphragm selector ② to fully
close the diaphragm.(Fig. 24)
4. Using the provided Allen wrench use the two centering
screws ③ to bring the bright spot in the center of the eld of
view.
5. Gradually open the diaphragm. The condenser is centered
when the diaphragm image is symmetrical to the eld of
view. (Fig. 25)
6. In normal use, open the diaphragm until it circumscribes the
eld of view.
10.8.2 E󰀨ects of the eld diaphragm
Field diaphragm adjusts the illuminated area to obtain a high
contrast image.
Set the diaphragm according to the objective in use until it
circumscribes the eld of view, in order to eliminate unnecessary
light to eyepieces.

Page 19
30-20%
10.8.3 Aperture diaphragm (AS)
1. Move the selector ① on the reected light illuminator in the
position corresponding to letter “R” (only B-510MET-R). (Fig.
23)
2. Completely pull out the aperture diaphragm selector ④to
fully close the diaphragm. (Fig. 26)
3. Remove one eyepiece.
4. While looking into the empty eyepiece sleeve, use the
provided Allen wrench into the centering screws ⑤to bring
the bright spot in the center of the eld of view. (Fig. 26)
5. The illuminator is centered when the diaphragm image is
symmetrical to the eld of view.
• The Numerical Aperture (N.A.) value of the aperture
diaphragm a󰀨ects the image contrast. Increasing or reducing
this value one can vary resolution, contrast and depth of
focus of the image.
• With low contrast specimens move the diaphragm lever to
about 70%-80% of the objective’s N.A. If necessary, remove
on eyepiece and, looking into empty sleeve, adjust the
condenser’s ring in order to obtain an image like the one in
Fig. 27.
FFig. 26ig. 26
FFig. 27ig. 27
④
⑤
FIELD OF VIEW
APERTURE
DIAPHRAGM 70-80%
FFig. 28ig. 28
②
③
①④
10.9 Centering the condenser (B-510MET-R)
1. Place the specimen on the stage, insert 10x objective into
the light path and focus.
2. Insert the front lens of the swing-out condenser ①. (Fig. 28)
3. Rotate the eld diaphragm ring ② in counterclockwise
direction, to fully close the diaphragm.
4. Rotate the condenser height adjustment knob ③ to focus the
edges of the diaphragm.
5. Rotate the two centering screws ④to bring the bright spot in
the center of the eld of view.
6. Gradually open the diaphragm. The condenser is centered
when the diaphragm image is symmetrical to the eld of view.
7. In normal use, open the diaphragm until it circumscribes the
eld of view.
FFig. 29ig. 29
①
10.10 Aperture diaphragm
• The Numerical Aperture (N.A.) value of the aperture
diaphragm a󰀨ects the image contrast. Increasing or reducing
this value one can vary resolution, contrast and depth of
focus of the image.
• With low contrast specimens set the numerical aperture value
① (printed on the condenser ring) to about 70%-80% of the
objective’s N.A. (Fig. 29) If necessary, remove on eyepiece
and, looking into empty sleeve, adjust the condenser’s ring in
order to obtain an image like the one in g. 27.
Example: with objective PLAN 40x / 0,65 set the scale to 0.65
x 0.8 = 0,52

Page 20
10.11 Use of lters
1. Insert the desired lter for the observation in the slot placed
in the left side of the illuminator (Fig. 30).
2. First click stop position leaves lter not inserted, while second
position inserts lter into the light path.
Filter Application
Blue Converts the source color temperature to
daylight
Green Increases the contrast in black and white pho-
tography
Yellow Contrast lter for the observation of wafers
and semiconductors
Frosted white Reduces lighting irregularities by creating a
homogeneous eld of view
FFig. 31ig. 31
FFig. 30ig. 30
10.12 Oblique illumination
1. After focusing the specimen with the desired objective, gently
move the oblique illumination sliders (Fig. 31).
2. Stop sliding when a shadow begins to obscurate the
specimen.
• In this way an oblique illumination e󰀨ect can be achieved,
that highlights reliefs and surface defects of your specimen.