Optika Italy B-810 Series User manual
Model
B-810
B-810 Series
INSTRUCTION MANUAL
Ver. 2.0 2019
Page 2
Table of Contents
1. Warning 3
2. Symbols and conventions 3
3. Safety Information 3
4. Intended use 3
5. Overview 4
5.1 Microscope body 4
5.2 Brighteldcondenser(M-1189/M-1191) 6
5.3 Brighteld/Darkeld/PhaseContrastcondenser(M-1152.NO) 6
5.4 Brighteld/PhaseContrastcondenser(M-1124.NO) 7
6. Unpacking 8
7. Assembling 8
7.1 Microscopeassembling 9
8. Brighteldobservationprocedures 11
9. Useofthemicroscope 12
9.1 Microscopeswitchon 12
9.2 Light intensity adjustment 12
9.3 Coarse focus tension adjustment 12
9.4
Coarse upper limit lever 13
9.5 Stage 13
9.6
Dioptricadjustment 13
9.7 Adjusting the interpupillary distance 14
9.8 Use of eye shields 14
9.9 Lightpathselection 14
9.10 Centering the condenser 15
9.11 Effectsoftheelddiaphragm 15
9.12 Aperturediaphragm 15
9.13 Useofoilimmersionobjective 16
10. Brighteld/Darkeld/PhaseContrastCondenser 17
10.1 BrighteldObservation(BF) 17
10.2 DarkeldObservation(DF) 17
10.3 PhaseContrastObservation(PH) 18
10.4 Useofthegreenlter 19
11. Brighteld/PhaseContrastCondenser 19
11.1 BrighteldObservation(BF) 20
11.2 PhaseContrastObservation(PH) 20
11.3 Useofthegreenlter 20
12. Microphotography 21
12.1 Use of C-mount cameras 21
12.2 UseofReexcameras 21
13. Maintenance 22
14. Troubleshooting 23
Equipment disposal 25
Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of
maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you
that this manual contains important information on safety and maintenance, and that it must therefore be made
accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses
that does not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICALSHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
AvoidingElectricalShock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the ope-
ration voltage of the equipment and that the lamp switch is in off position. Users should observe all safety regu-
lations of the region. The equipment has acquired the CE safety label. However, users have full responsibility to
use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure safe
operation of the unit.
4. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVDModels
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the
subject.
Page 4
5. Overview
PHOTO/TV
PORT
DIOPTER
ADJUSTMENT
RING
NOSEPIECE
COARSE
UPPER
LIMIT LEVER
OBJECTIVES
FIELD DIAPHRAGM
BRIGHTNESS
ADJUSTMENT KNOB
OBSERVATION
HEAD
CONDENSER
HEIGHT
ADJUSTMENT
KNOB
5.1 Microscope body
MAIN SWITCH
Page 5
EYEPIECES
STAGE
CONDENSER
BINOCULAR
HEAD
X/Y MOVEMENT
KNOBS
CONDENSER
CENTERING SCREWS
TENSION ADJUSTMENT
RING
SLIDE HOLDER
FINE FOCUS
KNOB
COARSE FOCUS
KNOB
Opposite side
LIGHT PATH
SELECTOR
Page 6
5.2 Brighteldcondenser(M-1189/M-1191)
SWING-OUT LEVER
APERTURE
DIAPHRAGM
SWING-OUT
FRONT LENS
5.3 Brighteld/Darkeld/PhaseContrastcondenser(M-1152.NO)
APERTURE DIAPHRAGM
LEVER (LOWER PART)
PHASE RING
CENTERING SCREWS
CONDENSER TURRET
(BF - DF - 10/20 - 40 - 100)
Page 7
5.4 Brighteld/PhaseContrastcondenser(M-1124.NO)
PHASE RING 40
APERTURE DIAPHRAGM
BRIGHTFIELD / PHASE
CONTRAST SLIDER
PHASE RING 10/20
PHASE RING
CENTERING
SCREWS
PHASE RING
CENTERING
SCREWS
Page 8
6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or
other residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
7. Assembling
Once opened the box, the microscope parts are the following:
① Microscope frame
② Eyepieces
③ Objectives
④ Observation head
⑤ Condenser (according to the conguration)
⑥ Immersion oil (if 100x objectibve is included
into the conguration)
⑦ Allen wrench
⑧ Dust cover
⑨ Power supply
⑩ Green lter (only for PH conguration)
⑪ Centering telescope (only for PH congura-
tion)
③
⑤
⑦
⑧
⑨
①
②
④
⑥
⑩
⑪
Page 9
7.1 Microscopeassembling
1. Insert the optical head above the stand and
tighten the screw. (Fig. 1)
• Holdtheheadwithonehandduringthelocking
in order to avoid that the head falls.
2. Insert both eyepieces into the tubes of the optical
head. (Fig. 2)
3. Insert the condenser under the stage. Check that
it is correctly inserted in its housing (under the
condenser there is a plug that must completely
enter into the guide of the condenser holder).
(Fig. 3)
4. Tighten the condenser xing screw ①.
5. Screw each objective into the thread of
the nosepiece, clockwise with increasing
magnication. (Fig. 4)
Fig. 1
Fig. 2
Fig. 4
Fig. 3
①
Page 10
Fig. 5
6. Insert the power supply jack in the connector
placed at the rear side of the microscope. (Fig. 5)
Page 11
8. Brighteldobservationprocedures
Main switch
(Used commands) (Chapter)
Slide holder
X/Y movement knobs
Nosepiece
Binocular tube
Diopter adjustment ring
Condenser height adjustment knob
Condenser centering screws
Aperture diaphragm
field diaphragm
Nosepiece
Brightness adjustment knob
Coarse and fine focus knobs
Coarse and fine focus knobs
Set the main swith to “1” and adjust light intensity.
Place a slide on the stage.
Insert 10x objective into the light path
Focus the specimen
Begin observation
Adjust aperture and field diaphragms
Insert in the light path the desired objective and focus the
specimen
Adjust interpupillary distance
Adjust diopter
Center optical axis
Adjust brightness
(5/9)
(9)
(5)
(5)
(5)
(5)
(5)
(9)
(9)
(9)
(9)
(9)
(9)
(5/9)
Brightness adjustment knob (5/9)
Page 12
9. Useofthemicroscope
9.1 Microscopeswitchon
Move the main switch ①, located on the left side of
the stand, to position “1” to turn on the microscope.
(Fig. 6)
9.2 Light intensity adjustment
Operate on the light intensity adjustment knob ② to
increase / decrease the illumination voltage. (Fig. 7)
9.3 Coarse focus tension adjustment
The tension of the coarse focusing knob is factory
preset.
1. To modify the tension according to personal’s
needs, rotate the ring
③
. (Fig. 8)
• Clockwise rotation increases the tension.
• If the tension is too loose, the stage could go
lower by itself or the focus easily lost after ne
adjustment. In this case, rotate the knob in order
to increase the tension
.
Fig. 6
Fig. 7
Fig. 8
③
①
②
Page 13
9.4
Coarse upper limit lever
The upper limit knob has two functions: prevent the
contact between slide and objective and acts as
“focus memory”.
1. After focussing the specimen, pull the lever
①
toward the front of the microscope and lock it.
(Fig. 9).
• IIn this way the focus upper limit is set.
2. Now one can lower the stage with coarse focus
knob, replace the specimen and raise again the
stage up to the upper limit: specimen will be in
approximate focus and will need a ne adjustment
to get the proper focus..
• Finefocusmovement isnotaffected bythe
coarsefocuslock.
• To unlock, move the lever in the opposite
directiontotheoneusedforthelocking.
• Two stoppers ②areinsertedontheframe.DO
NOTREMOVETHETWOSTOPPERS.
9.5 Stage
Stage accepts standard slides 26 x 76 mm, thickness
1,2 mm with coverlside 0,17mm.
It is possible to place two slides side by side on the
stage.
• Open the spring arm of the slide holder
③
and place frontally the slides on the stage.
(Fig.10)
• Gently release the spring arm of the slide
holder.
• A sudden release of the the spring arm could
cause the falling of the slide.
9.6
Dioptricadjustment
1. Look into the right eyepiece with your right eye
only, and focus on the specimen.
2. Look into the left eyepiece with your left eye
only. If the image is not sharp, use the dioptric
adjustment ring ④ to compensate. (Fig. 11)
• Theadjustmentrangeis±5diopter.Thenumber
indicated on the adjustment ring graduation
should correspond to the operator’s dioptric
correction.
Fig. 10
Fig. 11
④
③
Fig. 9
①
LOCK
UNLOCK
②
Page 14
9.7 Adjusting the interpupillary distance
Observing with both eyes, hold the two eyepiece
prism assemblies. Rotate them around their common
axis until the elds of view coincide.
• The graduation on the interpupillary distance
indicator ①, pointed by the spot “.” on the
eyepieceholder,showsthedistancebetween
theoperator’seyes.(Fig.12)
The range of the interpupillary distance is 48-75mm.
9.8 Use of eye shields
• Use with eyeglasses
Fold rubber eyeshields with both hands. Folded
eyeshields avoid scratching the lenses of eyeglasses.
(Fig. 13)
• Use without eyeglasses
Raise eye shields and observe at the microscope
placing eyes to the shields, avoiding external light to
disturb the observation. (Fig. 14)
9.9 Lightpathselection
The observation head is equipped with an optical
path selector that allows the light to be distributed to
the eyepieces and to the photo / TV port.
1. Fully insert the selector ② to get 100% of the light
to the eyepieces. (Fig. 15)
• In this case the photo / TV port is not enabled.
2. Pull out the selector to enable the light also at the
photo / TV port. The light split in this case will be
50% to the eyepieces and 50% to the photo / TV
port.
Fig. 13
Fig. 14
Fig. 12
①
Fig. 15
②
Page 15
9.10 Centering the condenser
1. Place the specimen on the stage, insert 10x
objective into the light path and focus.
2. Insert the front lens of the swing-out condenser
①. (Fig. 16)
3. Rotate the eld diaphragm ring ②in
counterclockwise direction, to fully close the
diaphragm.
4. Rotate the condenser height adjustment knob ③
to focus the edges of the diaphragm.
5. Rotate the two centering screws ④to bring the
bright spot in the center of the eld of view.
6. Gradually open the diaphragm. The condenser
is centered when the diaphragm image is
symmetrical to the eld of view. (Fig. 17)
7. In normal use, open the diaphragm until it
circoscribes the eld of view.
9.11 Effectsoftheelddiaphragm
Field diaphragm adjusts the illuminated area to obtain
a high contrast image.
Set the diaphragm according to the objective in
use until it circoscribes the eld of view, in order to
eliminate unnecessary light to eyepieces.
9.12 Aperturediaphragm
• The Numerical Aperture (N.A.) value of the
aperture diaphragm affects the image contrast.
Increasing or reducing this value one can vary
resolution, contrast and depth of focus of the
image.
• With low contrast specimens set the numerical
aperture value ⑤ (printed on the condenser ring)
to about 70%-80% of the objective’s N.A. (Fig. 18)
If necessary, remove on eyepiece and, looking
into empty sleeve, adjust the condenser’s ring in
order to obtain an image like the one in g. 19.
Example:withobjectivePLAN40x/0,65setthe
scaleto0.65x0.8=0,52
Fig. 17
Fig. 40
Fig. 19
IRIS
DIAPHRAGM
FIELD OF VIEW
30-20%
70-80%
Fig. 18
⑤
Fig. 16
②
③
①④
Page 16
9.13 Use of oil immersion objective
1. Focus the specimen with a low power objective.
2. Lower the stage (remembering to lock the coarse
upper limit lever).
3. Put a drop of oil (provided) on the area of the
specimen to be observed. (Fig. 20)
• Make sure that there are no oil bubbles.Air
bubbles in the oil damage the image quality.
• To check for bubbles: remove an eyepiece, fully
open the aperture diaphragm and observe the
objective exit pupil. (The pupil must be round and
bright).
• To remove the bubbles, gently move the
nosepiece to the right and left to move the
immersion objective a few times and allow the air
bubbles to move.
4. Insert immersion objective.
5. Return the stage to the upper focusing point and
obtain an optimal focus using the ne focus knob.
6. After use, gently remove the oil with a soft paper
towel or a lightly moistened optic paper with a
mixture of ethyl ether (70%) and absolute ethyl
alcohol (30%).
• Theimmersionoil,ifnotimmediatelycleaned,
couldcrystallizecreatingaglass-likelayer.In
this situation the observation of the specimen
wouldbedifcultifnotimpossibleduetothe
presence of an additional thickness on the
objective.
Fig. 20
Page 17
10.1 BrighteldObservation(BF)
1. Rotate the condenser turret to insert the “BF” position.
2. Now repeat the steps described in the chapter “Brighteld observation procedure”.
10.2 DarkeldObservation(DF)
1. Rotate the condenser turret to insert the “DF”position.
• Byinsertingthedarkeldring,theaperturediaphragmopensautomatically.Thisisadesiredeffect
and should not be considered a defect.
2. Place a specimen on the stage and focus.
3. Observing into eyepieces raise or lower the condenser until a homogeneous illumination of the specimen can
be achieved, thus obtaining a proper darkeld effect.
• Darkeld requires a huge amount of light. Switching from darkeld to brighteld, one could be
dazzled.DonotkeepyoureyesontheeyepieceswhenmovingthecondenserturretfromDFtoBF.
• “Dry”darkeldobservation,thatis,withouttheuseofoil,isonlypossiblewithobjectiveswithN.A.
lowerthan0,7.
• Observingindarkeld,itmaybenecessarytoraisethecondenserfromthenormalpositiontoobtain
a more homogeneous illumination. This is not a defect.
10. Brighteld/Darkeld/PhaseContrastCondenser
The M-1152.NO condenser allows observation in bright eld, dark eld and phase contrast.
Observation mode Condenser Turret position
Brightfield BF (Fig. 20)
Darkeld DF (Fig. 21)
Phase contrast 10x 10/20 (Fig.22)
Phase contrast 20x 10/20 (Fig.22)
Phase contrast 40x 40 (Fig. 23)
Phase contrast 100x 100 (Fig. 24)
Fig. 20 Fig. 21
Fig. 24Fig. 23Fig. 22
Page 18
Fig. 25
Fig. 26
Fig. 28
Fig. 27
UPPER PART OF
CENTERING
TELESCOPE
CONDENSER
PHASE RING
(BRIGHT) ②
OBJECTIVE
PHASE
RING
(DARK) ③
①
10.3 PhaseContrastObservation(PH)
1. Center the condenser as already described in the
chapter 9.10.
• This condenser does not have a swing-out lens,
so the operation described in step 2 is not needed.
2. Rotate the condenser turret to insert the “10/20”
position.
3. nsert 10x objective into the light path.
• By inserting any phase ring, the aperture
diaphragm opens automatically. This is a de-
sired effect and should not be considered a
defect.
4. Place a specimen on the stage and focus.
5. Remove one eyepiece and insert the centering
telescope. (Fig. 25)
6. Rotate the upper part of the centering telescope
until the two phase rings (one dark and one bright)
visible in the telescope are in focus. (Fig. 26)
7. Using centering screws on the condenser ① (Fig.
27), center the phase rings to make the bright ring
② be concentric to the dark ring ③. (Fig. 28)
8. Insert 20x objective (do not rotate the condenser
turret) and check the centering of the two rings.
9. Repeat the same operation with other objectives
to check the ring centering: 40x objective – turret
position “40”, 100x objective – turret position
“100”.
10. At the end remove the centering telescope,
reinstall the eyepiece and begin observation.
• With40xand100xobjectivesitmaybeuseful
to slightly raise the condenser, to obtain a
better projection of the phase rings. This is
not a defect.
11. With the 4X objective, the condenser could
haveadarkhaloattheperipheryoftheeldof
view. This is not to be considered a defect.
Page 19
10.4 Useofthegreenlter
• The green lter is used to increase the contrast
of the image during phase contrast observation.
• Place the lter on the eld lens of the microscope
(Fig. 29) and begin the observation.
• For observation in brighteld or darkeld it is advi-
sable to remove the lter from the optical path.
Fig. 29
11. Brighteld/PhaseContrastCondenser
Slider condenser M-1124.NO allows observation in bright eld and phase contrast with objectives 10X, 20X and
40X.
Observation mode Slider position
Brighteld O (Fig. 30)
Phase contrast 10x 10/20 (Fig. 31)
Phase contrast 20x 10/20 (Fig. 31)
Phase contrast 40x 40 (Fig. 32)
Fig. 32
Fig. 31
Fig. 33
Page 20
11.1 BrighteldObservation(BF)
1. Move the condenser slider to the middle position
to insert the empty position. (Fig. 33)
2. Now repeat the steps described in the chapter
“Brighteld observation procedure”.
11.2 PhaseContrastObservation(PH)
1. Center the condenser as already described in the
chapter 9.10.
• This condenser does not have a swing-out lens,
so the operation described in step 2 is not needed.
2. Move the condenser slider to the right to insert
the phase ring 10 (Fig. 34) or to the left to insert
the phase ring 40. (Fig. 35)
3. Insert the corresponding objective in to the light
path.
4. Open the aperture diaphragm.
5. Place a specimen on the stage and focus.
6. Remove one eyepiece and insert the centering
telescope. (Fig. 25)
7. Rotate the upper part of the centering telescope
until the two phase rings (one dark and one bright)
visible in the telescope are in focus. (Fig. 25-26)
8. Using the centering screws on the slider ① (Fig.
36), cener the rings as already described in
chapter 10.3.
9. At the end remove the centering telescope,
reinstall the eyepiece and begin observation.
• With40xand100xobjectivesitmaybeuseful
to slightly raise the condenser, to obtain a
better projection of the phase rings. This is
not a defect.
• With the 4X objective, the condenser could
haveadarkhaloattheperipheryoftheeld
of view. This is not to be considered a defect.
11.3 Useofthegreenlter
• The green lter is used to increase the contrast
of the image during phase contrast observation.
• Place the lter on the eld lens of the microscope
(Fig. 29) and begin the observation.
• For observation in brighteld it is advisable to
remove the lter from the optical path.
Fig. 36
①
Fig. 34
Fig. 35
Fig. 36
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