Optika Italy B-810 Series User manual

Model
B-810
B-810 Series
INSTRUCTION MANUAL
Ver. 2.0 2019

Page 2
Table of Contents
1. Warning 3
2. Symbols and conventions 3
3. Safety Information 3
4. Intended use 3
5. Overview 4
5.1 Microscope body 4
5.2 Brighteldcondenser(M-1189/M-1191) 6
5.3 Brighteld/Darkeld/PhaseContrastcondenser(M-1152.NO) 6
5.4 Brighteld/PhaseContrastcondenser(M-1124.NO) 7
6. Unpacking 8
7. Assembling 8
7.1 Microscopeassembling 9
8. Brighteldobservationprocedures 11
9. Useofthemicroscope 12
9.1 Microscopeswitchon 12
9.2 Light intensity adjustment 12
9.3 Coarse focus tension adjustment 12
9.4 Coarse upper limit lever 13
9.5 Stage 13
9.6 Dioptricadjustment 13
9.7 Adjusting the interpupillary distance 14
9.8 Use of eye shields 14
9.9 Lightpathselection 14
9.10 Centering the condenser 15
9.11 Effectsoftheelddiaphragm 15
9.12 Aperturediaphragm 15
9.13 Useofoilimmersionobjective 16
10. Brighteld/Darkeld/PhaseContrastCondenser 17
10.1 BrighteldObservation(BF) 17
10.2 DarkeldObservation(DF) 17
10.3 PhaseContrastObservation(PH) 18
10.4 Useofthegreenlter 19
11. Brighteld/PhaseContrastCondenser 19
11.1 BrighteldObservation(BF) 20
11.2 PhaseContrastObservation(PH) 20
11.3 Useofthegreenlter 20
12. Microphotography 21
12.1 Use of C-mount cameras 21
12.2 UseofReexcameras 21
13. Maintenance 22
14. Troubleshooting 23
Equipment disposal 25

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1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of
maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you
that this manual contains important information on safety and maintenance, and that it must therefore be made
accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses
that does not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICALSHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
  AvoidingElectricalShock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the ope-
ration voltage of the equipment and that the lamp switch is in off position. Users should observe all safety regu-
lations of the region. The equipment has acquired the CE safety label. However, users have full responsibility to
use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure safe
operation of the unit.
4. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVDModels
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the
subject.

Page 4
5. Overview
PHOTO/TV
PORT
DIOPTER
ADJUSTMENT
RING
NOSEPIECE
COARSE
UPPER
LIMIT LEVER
OBJECTIVES
FIELD DIAPHRAGM
BRIGHTNESS
ADJUSTMENT KNOB
OBSERVATION
HEAD
CONDENSER
HEIGHT
ADJUSTMENT
KNOB
5.1 Microscope body
MAIN SWITCH

Page 5
EYEPIECES
STAGE
CONDENSER
BINOCULAR
HEAD
X/Y MOVEMENT
KNOBS
CONDENSER
CENTERING SCREWS
TENSION ADJUSTMENT
RING
SLIDE HOLDER
FINE FOCUS
KNOB
COARSE FOCUS
KNOB
Opposite side
LIGHT PATH
SELECTOR

Page 6
5.2 Brighteldcondenser(M-1189/M-1191)
SWING-OUT LEVER
APERTURE
DIAPHRAGM
SWING-OUT
FRONT LENS
5.3 Brighteld/Darkeld/PhaseContrastcondenser(M-1152.NO)
APERTURE DIAPHRAGM
LEVER (LOWER PART)
PHASE RING
CENTERING SCREWS
CONDENSER TURRET
(BF - DF - 10/20 - 40 - 100)

Page 7
5.4 Brighteld/PhaseContrastcondenser(M-1124.NO)
PHASE RING 40
APERTURE DIAPHRAGM
BRIGHTFIELD / PHASE
CONTRAST SLIDER
PHASE RING 10/20
PHASE RING
CENTERING
SCREWS
PHASE RING
CENTERING
SCREWS

Page 8
6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or
other residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
7. Assembling
Once opened the box, the microscope parts are the following:
① Microscope frame
② Eyepieces
③ Objectives
④ Observation head
⑤ Condenser (according to the conguration)
⑥ Immersion oil (if 100x objectibve is included
into the conguration)
⑦ Allen wrench
⑧ Dust cover
⑨ Power supply
⑩ Green lter (only for PH conguration)
⑪ Centering telescope (only for PH congura-
tion)
③
⑤
⑦
⑧
⑨
①
②
④
⑥
⑩
⑪

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7.1 Microscopeassembling
1. Insert the optical head above the stand and
tighten the screw. (Fig. 1)
• Holdtheheadwithonehandduringthelocking
in order to avoid that the head falls.
2. Insert both eyepieces into the tubes of the optical
head. (Fig. 2)
3. Insert the condenser under the stage. Check that
it is correctly inserted in its housing (under the
condenser there is a plug that must completely
enter into the guide of the condenser holder).
(Fig. 3)
4. Tighten the condenser xing screw ①.
5. Screw each objective into the thread of
the nosepiece, clockwise with increasing
magnication. (Fig. 4)
Fig. 1
Fig. 2
Fig. 4
Fig. 3
①

Page 10
Fig. 5
6. Insert the power supply jack in the connector
placed at the rear side of the microscope. (Fig. 5)