Optika Italy LAB Series User manual

Version: 2
Issued: 10, 01, 2017
Model
LAB-10
LAB-20
LAB-30
LAB Series
INSTRUCTION MANUAL

Page 2
Table of Contents
Warning
Symbols and conventions
Safety Information
Intended use
List of accessories and spare parts
Overview
Unpacking
Assembling
Maintenance
Troubleshooting
Equipment disposal

Page 3
Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of mainte-
nance. It is built to high optical and mechanical standards and to withstand daily use. We remind you that this
manual contains important information on safety and maintenance, and that it must therefore be made acces-
sible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses that does
not comply with this manual.
Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibil-
ity to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
Intended use
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.

Page 4
List of accessories and spare parts
CAT. NO. DESCRIPTION
ST-001.1 Eyepieces (pair) wf5x/22mm.
ST-401 Eyepieces (pair) WF10x/20 mm.
ST-402 Eyepieces (pair) WF15x/15 mm.
ST-403 Eyepieces (pair) WF20x/10 mm.
ST-405 Eyepiece micrometer WF10x/20mm.
ST-085 Additional lens 0.5x (w.d. 165mm) (Only for LAB-20 and LAB-30.)
ST-086 Additional lens 1.5x (w.d. 47mm) (Only for LAB-20 and LAB-30.)
ST-415 Eyecups (pair).
DC-002 Plastic dust cover, medium
ST-092 Protective glass for stereohead.
ST-417 Round plastic stage
M-114 C-Mount adapter for 1/2” sensor.
M-115 C-Mount adapter for 1/3” sensor.
M-116 C-Mount adapter for 2/3” sensor.
M-173 Photo adapter for APS-C and Full Frame Reex cameras.
M-113.1 Ring adapter, 30mm (for monocular and binocular microscopes).
ST-418 Focusable C-Mount adapter for 1/3” sensor.
ST-419 Focusable C-Mount adapter for 1/2” snsor.
15104 Lens cleaner, 50ml.
VP-LAB IQ/OQ/PQ Validation Protocols.

Page 5
Overview
DIOPTRIC
COMPENSATION
EYEPIECE
FOCUSING
KNOB
POWER
SWITCH
LIGHT
INTENSITY
ADJUSTMENT
LIGHT
INTENSITY
ADJUSTMENT
OBJECTIVE
CLIPS

Page 6
Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Assembling
Once you open the box, these are the microscope’s components:
① Stereo Head
② Microscope stand
③ Eyepieces
④ Black/white
contrast disc
⑤ Power cable
③④
⑤
①
②
Fig.1
Fig.2
1. Insert the stereo head into the holder on
the stand, and tighten the side screw.
(Fig.1)
2. Insert the eyepieces into the tubes of the
head. (Fig.2)

Page 7
Fig.3
Fig.5Fig.4
Fig.6
1. Place the specimen on the stage
Place the specimen on the microscope
stage and lock the specimen using the
slide-clamp if it is necessary. Ensure that
the specimen is centred over the stage
opening. (Fig.3)
2. Turn on the light
The microscope comes with an electrical
illuminator. Insert the plug of the cable into
the power socket and turn on the switch on
the main body and select your light source.
(Fig.4) Turn the light intensity control to a
brightness suitable for observation. (Fig.5)
3. Adjust interpupillary distance
Hold the right and left parts of the
observation head by both hands and
adjust the interpupillary distance by
turning the two parts until one circle of
light can be seen. If two circles appear,
the interpupillary distance is too big, and
if two overlapped circles appear, the
interpupillary distance is too small. (Fig.6)
4. Focus
Rotate the focusing knob to bring the
sample into focus with the highest
magnication. (Fig.7)
5. Dioptric compensation
This compensation makes it possible
for people with glasses to adjust the
microscope to their eyes and use the
microscope without glasses. Turn the
zoom down to the lowest magnication.
Adjust the diopter compensation ring of the
right eyepiece tube until the image of the
right eyepiece is clear and sharp. Repeat
the procedure for the left eyepiece. Then,
check the focus of the image for the whole
zoom range. It should now be perfectly
parfocal (focus is always maintained during
the change of magnication). (Fig.8)
Using the microscope
Fig.7
Fig.8

Page 8
6. Magnication
Select the desired magnication by
adjusting the zoom knob. Change the
eyepieces and/or add an appropriate
magnifying objective lens if necessary.
(Fig.9)
Total magnication used can be calculated
as:
Eyepiece magnication x Zoom
magnication x Objective lens
magnication.
7. Contrast disc
You can use the black/white disc in order
to enhance the contrast of the image when
using the incident illumination. (Fig.10).
If you want to look at bright opaque
objects, place the disc with the black side
up. (Fig.11)
8. Working distance
Normal working distance for the standard
conguration is 80 mm (LAB-10) and
85mm (LAB-20 & LAB-30). (Fig.12)
9. Video capturing
Two kinds of observation heads, binocular
or trinocular, are available for the LAB
series. They can all be connected to
cameras via an adaptor.
Please refer to the adaptor and camera
manuals for further details.
Fig.9
Fig.10
Fig.11
Fig.12
W.D.

Page 9
Maintenance
Microscopy environment
This microscope is recommended to be used in a clean, dry and shock free environment with a temperature of
5°-40°C and a maximum relative humidity of 75 % (non condensing). Use a dehumidier if needed.
To think about when and after using the microscope
• The microscope should always be kept vertically when moving it and be careful so that no
moving parts, such as the eyepieces, fall out.
• Never mishandle or impose unnecessary force on the microscope.
• Never attempt to service the microscope yourself.
• After use, turn o󰀨 the light immediately, cover the microscope with the included
dust-cover, and keep it in a dry and clean place.
Electrical safety precautions
• Before plugging in the power supply, make sure that the supplying voltage of your region
matches with the operation voltage of the equipment and that the lamp switch is in o󰀨-
position.
• Users should observe all safety regulations of the region. The equipment has acquired
the CE safety label. However, users do have full responsibility to use this equipment safely.
Cleaning the optics
• If the optical parts need to be cleaned try rst to: use compressed air.
• If that is not su󰀩cient: use a soft lint-free piece of cloth with water and a mild detergent.
• And as a nal option: use the piece of cloth moistened with a 3:7 mixture of ethanol and ether.
Note: ethanol and ether are highly ammable liquids. Do not use them near a heat source, near sparks or
near electric equipment. Use these chemicals in a well ventilated room.
• Remember to never wipe the surface of any optical items with your hands. Fingerprints can damage the
optics.
• Do not disassemble objectives or eyepieces in attempt to clean them.
For the best results, use the OPTIKA cleaning kit (see catalogue).
If you need to send the microscope to Optika for maintenance, please use the original packaging.

Page 10
Troubleshooting
Review the information in the table below to troubleshoot operating problems.
PROBLEM CAUSE SOLUTION
I. Optical Section:
The illumination is ON, but the eld of
view is dark.
The plug is not connected to the il-
lumination
Connect the cable
The brightness is too low Adjust to a proper setting
The edge of the eld of view is
vignetted or the brightness is
asymmetric.
The incident illuminator is not cor-
rectly oriented
Change the angle of the incident illuminator
Dust and stains can be seen in the
eld of view.
There are stains and dust on the
specimen
Clean the specimen
There are stains and dust on the
eyepiece
Clean the eyepiece
Poor image quality:
The image is not sharp
The contrast is not high
The details are not clear
The lenses (condenser, objective,
eyepieces is dirty
Thoroughly clean all the optical system
One side of the image is out of focus. The specimen is out of place (tilted) Place the specimen at on the stage.
II. Mechanical Section:
The coarse focus knob is hard to
turn.
The tension adjustment collar is too
tight
Loosen the tension adjustment collar
The focus is unstable. The tension adjustment collar is too
loose
Tighten the tension adjustment collar
III. Electric section:
The LED doesn’t turn on. No power supply Check the power cord connection
The brightness is not enough The brightness adjustment is low Adjust the brightness
The light blinks The power cord is poorly connected Check the power cord
IV. Viewing tube assembly:
The eld of view of the two eyes is
di󰀨erent
The interpupillar distance is not cor-
rect
Adjust the interpupillar distance
The dioptric correction is not right Adjust the dioptric correction
The viewing technique is not cor-
rect, and the operator is straining the
eyesight
When look into the objective, do not stare
at the specimen but look at the whole eld
of view. Periodically, move the eyes away
to look at a distant object, then back into
the objective
V. Microphotography and video:
The image is unfocused Incorrect focussing Adjusting the focus system as in the
present manual
The edge of the image is unfocussed To some degree, it is inherent to the
nature of achromatic objectives
The problem can be minimized by a correct
setting of the aperture diaphragm
Bright patches appear on the image Stray light is entering the microscope
through the eyepieces and through
the camera viewnder
Cover the eyepieces and the viewnder
with a dark cloth

Page 11
Equipment disposal
Art.13 Dlsg 25 july 2005 N°151. “According to directives 2002/95/EC, 2002/96/EC and 2003/108/EC relating
to the reduction in the use of hazardous substances in electrical and electronic equipment and waste disposal.”
The basket symbol on equipment or on its box indicates that the product at the end of its useful life should be
collected separately from other waste.
The separate collection of this equipment at the end of its lifetime is organized and managed by the producer.
The user will have to contact the manufacturer and follow the rules that he adopted for end-of-life equipment
collection.
The collection of the equipment for recycling, treatment and environmentally compatible disposal, helps to prevent
possible adverse effects on the environment and health and promotes reuse and/or recycling of materials of the
equipment.
Improperdisposal of the product involves the application of administrative penalties as provided bythe laws in force.

Page 12

Modello
LAB-10
LAB-20
LAB-30
Serie LAB
MANUALE D’ISTRUZIONI
Versione: 2
Emesso il: 10, 01, 2017

Pagina 14
Indice Contenuti
Avvertenza
Simboli
Informazioni sulla sicurezza
Utilizzo previsto
Accessori sostituibili e parti di ricambio
Descrizione dello strumento
Apertura della confezione
Assemblaggio
Manutenzione
Soluzioni per eventuali problemi
Smaltimento

Pagina 15
Avvertenza
Questo microscopio è uno strumento scientico di alta precisione, progettato per durare a lungo con una mini-
ma manutenzione; la realizzazione è secondo i migliori standard ottici e meccanici, per poter essere utilizzato
quotidianamente.
Vi ricordiamo che questo manuale contiene informazioni importanti per la sicurezza e per la manutenzione dello
strumento, e deve quindi essere messo a disposizione di coloro che lo utilizzeranno.
Decliniamo ogni responsabilità derivante da un utilizzo dello strumento non indicato nel presente manuale.
Simboli
La seguente tabella riporta i simboli utilizzati in questo manuale.
PERICOLO
Questo simbolo indica un rischio potenziale ed avverte di procedere con cautela.
SHOCK ELETTRICO
Questo simbolo indica un rischio di shock elettrico.
Informazioni sulla sicurezza
Per evitare shock elettrici
Prima di collegare il cavo di alimentazione alla presa elettrica, assicurarsi che il voltaggio della rete locale coin-
cida con il voltaggio dello strumento e che l’interruttore dell’illuminazione sia nella posizione “O󰀨”.
Gli utenti dovranno seguire tutte le norme di sicurezza locali. Lo strumento è certicato CE. In ogni caso, gli
utilizzatori sono gli unici responsabili per un utilizzo sicuro dello strumento. Per l’utilizzo in sicurezza dello stru-
mento è importante attenersi alle seguenti istruzioni e leggere il manuale in tutte le sue parti.
Utilizzo previsto
Solo per ricerca. Non è previsto alcun utilizzo di questo strumento per uso diagnostico.

Pagina 16
Accessori sostituibili e parti di ricambio
CAT. NO. DESCRIZIONE
ST-001.1 Oculari (coppia) WF5x/22mm
ST-401 Oculari (coppia) WF10x/20 mm
ST-402 Oculari (coppia) WF15x/15 mm
ST-403 Oculari (coppia) WF20x/10 mm.
ST-405 Oculare micrometrico WF10x/20mm
ST-085 Lente addizionale 0,5x (w.d. 165mm) (Solo per LAB-20 e LAB-30.)
ST-086 Lente addizionale 1,5x (w.d. 47mm) (Solo per LAB-20 e LAB-30.)
ST-415 Paraocchi (coppia)
DC-002 Copertina anti-polvere in plastica, media
ST-092 Vetro protettivo per testata stereo
ST-417 Disco in plastica
M-114 Adattatore C-Mount per sensore 1/2”
M-115 Adattatore C-Mount per sensore 1/3”
M-116 Adattatore C-Mount per sensore 2/3”
M-173 Adattatore fotograco per macchine fotograche reex APS-C e Full Frame
M-113.1 Anello adattatore, 30mm (per microscopi monoculari e binoculari)
ST-418 Adattatore fochettabile C-Mount per sensorer 1/3”.
ST-419 Adattatore fochettabile C-Mount per sensore1/2”.
15104 Kit di pulizia, 50ml.
VP-LAB IQ/OQ/PQ Protocollo di validazione.

Pagina 17
Descrizione dello strumento
ANELLI DI
COMPENSAZIONE
DIOTTRICA
OCULARI
MANOPOLA
DI MESSA A
FUOCO
PINZETTE PER
SOSTEGNO
CAMPIONI
REGOLAZIONE
INTENSITÀ
LUMINOSA
REGOLAZIONE
INTENSITÀ
LUMINOSA
OBIETTIVI
PULSANTE
DI
ACCENSIONE

Pagina 18
Apertura della confezione
Il microscopio si trova in un imballaggio di polistirolo espanso stampato. Dopo aver tolto il nastro adesivo da
tutti gli imballi, sollevare la metà superiore dell’imballaggio. Fare attenzione a non far cadere o danneggiare le
componenti ottiche (obiettivi e oculari). Estrarre il microscopio dal suo imballaggio con entrambe le mani (una
intorno al braccio e una intorno alla base) e appoggiarlo su un piano stabile.
Assemblaggio
Queste sono le componenti del microscopio che trovate quando aprite la scatola:
① Testata stereo
② Stativo del microscopio
③ Oculari
④ Disco di contrasto
bianco/ nero
⑤ Cavo alimentatore
③④
⑤
①
②
Fig.1
Fig.2
1. Inserire la testata stereo nell’anello di
ssaggio dello stativo e stringete la vite
laterale (Fig.1).
2. Inserite gli oculari nei portaoculari della
testata. (Fig.2)

Pagina 19
1. Posizionamento del campione
Posizionare il campione sul piano porta-
preparati del microscopio e bloccare
il vetrino con le apposite pinzette se
necessario. Assicurarsi che il vetrino si
trovi al centro del campo di osservazione.
(Fig.3)
2. Accensione della luce
Il microscopio è dotato di un illuminatore
elettrico. Inserire lo spinotto del cavo
nella presa di alimentazione e premere il
pulsante di accensione posto sullo stativo e
selezionare la sorgente luminosa. (Fig.4)
Regolare l’intensità della luce mediante
l’apposita manopola no ad ottenere una
luminosità adatta all’osservazione. (Fig.5)
3. Regolazione della distanza
interpupillare
Regolare la distanza interpupillare dei
portaoculari sulla testata no ad ottenere
la visione di un unico campo luminoso
circolare. Se appaiono due cerchi signica
che la distanza interpupillare è troppo
grande, mentre se si vedono due cerchi
sovrapposti signica che la distanza
interpupillare è troppo ravvicinata. (Fig.6)
4. Messa a fuoco
Ruotando l’apposita manopola mettere a
fuoco il vetrino con il maggior potere di
ingrandimento. (Fig.7)
5. Regolazione diottrica
Questo tipo di compensazione rende
possibile l’utilizzo del microscopio anche
senza l’uso di occhiali. Regolare lo zoom
al minimo ingrandimento. Ruotare l’anello
di compensazione diottrica dell’oculare
destro no a che l’immagine è denita e a
fuoco. Ripetere la procedura per l’oculare
sinistro. Quindi controllare la messa a
fuoco dell’immagine per tutta l’escursione
di zoom. Lo zoom deve risultare parafocale
(il fuoco viene sempre mantenuto variando
l’ingrandimento). (Fig.8)
Istruzioni per l’uso
Fig.3
Fig.5Fig.4
Fig.6
Fig.7
Fig.8

Pagina 20
6. Ingrandimento
Selezionare il potere di ingrandimento
desiderato mediante lo zoom. Cambiare
gli oculari e/o aggiungere un obiettivo da
ingrandimento se necessario. Per calcolare
l’ingrandimento totale applicato, si usi la
seguente formula: Ingrandimento oculare
x Ingrandimento zoom x Ingrandimento
obiettivo. (Fig.9)
7. Disco di contrasto bianco/ nero
E’ possibile utilizzare il disco di contrasto
bianco/ nero per aumentare il contrasto
dell’immagine quando si usa la luce
incidente (Fig.10). Se si vuole vedere
oggetti chiari e opachi, appoggiare sulla
base il piatto con il lato nero verso l’alto
(Fig.11).
8. Distanza di lavoro
La distanza di lavoro normale in una
congurazione standard è pari a 80 mm
(LAB-10) e 85mm (LAB-20 & LAB-30).
(Fig.12).
9. Cattura video
Sono disponibili due tipologie di testate
per la serie LAB: binoculare e trinoculare.
Entrambe possono essere connesse a
telecamere tramite un adattatore. Fare
riferimento al manuale della telecamera
per ulteriori dettagli.
Fig.9
Fig.10
Fig.11
Fig.12