Optika Italy IM-5 Series User manual

Ver. 1.3 2022
Model
IM-5MET
IM-5 Series
INSTRUCTION MANUAL

Page 2
Table of contents
1. Warning 3
2. Safety Information 3
3. Package content 4
4. Unpacking 5
5. Intended use 5
6. Symbols and conventions 5
7. Instrument description 6
8. Assembling 8
8.1 Installing the objectives 8
8.2 Use of nosepiece adapter rings 8
8.3 Removing the stage lock 9
8.4 Installing the stage insert 9
8.5 Installing the eyepieces 10
8.6 Installing the lamp housing 10
8.7 Installing the bulb 11
8.8 Connecting the power cord 11
9. Brightfield observation procedures 13
10. Use of the microscope in Brightfield (BF) 14
10.1 General switch on 14
10.2 Brightness adjustment 14
10.3 SwitchingBrighteldobservation 14
10.4 Adjust the interpupillary distance 15
10.5 Diopter adjustment 15
10.6 Use of eyeshields 15
10.7 Light path selection 16
10.8 Coarse focus tension adjustment 16
10.9 ECO button 16
10.10Centeringtheelddiaphragm(FS) 17
10.11Effectsoftheelddiaphragm 17
10.12 Centering the Aperture Diaphragm (AS) 17
10.13Useofcolorlters 18
11. Use of the microscope in Darkfield (DF) 18
11.1 SwitchingDarkeldobservation 18
12. Use of the microscope in Polarized light (PO) 19
13. Microphotography 20
13.1 Use of C-mount cameras 20
13.2 UseofReexcameras 20
14. Maintenance 21
15. Troubleshooting 22
Equipment disposal 24

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1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of maintenance. It
is built to high optical and mechanical standards and to withstand daily use. We remind you that this manual contains
important information on safety and maintenance, and that it must therefore be made accessible to the instrument users.
We decline any responsibility deriving from incorrect instrument use uses that does not comply with this manual.
2. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the operation voltage
of the equipment and that the lamp switch is in off position. Users should observe all safety regulations of the region. The
equipment has acquired the CE safety label. However, users have full responsibility to use this equipment safely. Please
follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.

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3. Package content
① Microscopebody
② Eyepieces
③ Stage
④ Objectives
⑤ Lamphousing
⑥ Daylightlter(LBD)
⑦ Analyzer
⑧ Polarizer
⑨ Filterholder
⑩ DICdummyslider
⑪ Powersupply
⑫ Powercord
⑬ Revolvingnosepiece
⑭ Nosepieceadapterrings
⑮ Dustcover
⑯ Stageclips
⑰ Allenwrench
⑱ Metalinsertforstage
⑪
⑩
③
④
⑤
⑬
⑭⑮
⑯⑰
⑥
⑦
⑧⑨
①
②
⑱
⑫

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4. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container and lift the
top half of the container. Take some care to avoid that the optical items (objectives and eyepieces) fall out and get dam-
aged. Using both hands (one around the arm and one around the base), lift the microscope from the container and put it
on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or other
residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
5. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the subject.
6. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.

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PHOTO/TV
PORT
DIOPTER
ADJUSTMENT
RING
EYEPIECE
LIGHT PATH
SELECTOR
LEVER
COARSE
FOCUS KNOB
FINE FOCUS
KNOB
BF/DF MIRROR
SELECTION LEVER
OBJECTIVE
LAMP HOUSING
MICROSCOPE
BODY
STAGE PLATE
ECO
BUTTON
7. Instrument description

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TENSION
ADJUSTMENT
COLLAR
BRIGHTNESS
ADJUSTMENT KNOB
MAIN SWITCH
X/Y AXIS KNOBS
APERTURE DIAPHRAGM
FIELD DIAPHRAGM
DIC SLIDER SLOT
NOSEPIECE
Opposite side

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8. Assembling
FFig. 1ig. 1
FFig. 2ig. 2
FFig. 4ig. 4
FFig. 3ig. 3
②
③
①
8.1 Installing the objectives
1. Turn the coarse focusing knob ① until the nosepiece reach-
es its lowest position. (Fig. 1)
• For a safe transport, the nosepiece is placed in the low-
est position and the tension adjustment collar ②is ad-
justed to the appropriate tension when the microscope
leaves the factory. (Fig. 1)
2. Screw the lowest magnication objective on to the nosepiece
from the right side, then turn the turret clockwise. Install the
other objectives in the same way, following the sequence
from low to high magnication.
• Note: the objectives can also be installed through the
stage opening. (Fig. 2)
• Clean the objectives regularly. In inverted microscopes, the
objectives are very sensitive to dust.
• To prevent dust and contamination from entering the micro-
scope, cover all the unused holes with dust caps ③. (Fig. 3)
• When operating, use the low magnication objective (5x or
10X) to search and focus the specimen, then switch to higher
magnications.
8.2 Use of nosepiece adapter rings
The nosepiece can allocate objectives for brighteld/darkeld
observation (BD).
However, it is possible to install objectives for brighteld using
the adapter rings.
1. Screw the adapter ring into the empty hole of the nosepiece.
(Fig. 4 - 5)

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2. Screw the objective into the adapter ring. (Fig. 6)
3. Perform the observation as usual.
• The brighteld objective cannot be used for darkeld,
therefore switching the mirror cube selector to “DF” could
bring to a completely dark image.
• Using the adapter rings it could happen an imperfect
parfocality between brighteld and brighteld/darkeld
objectives. This is not a defect.
FFig. 5ig. 5
FFig. 6ig. 6
FFig. 7ig. 7
①
8.3 Removing the stage lock
1. Remove the 4 screws of the metal retaining plate ①, using
the supplied Allen screwdriver. (Fig. 7)
8.4 Installing the stage insert
• Make sure that the plate is horizontal.
1. Install the stage insert in the stage opening. (Fig. 8)
FFig. 8ig. 8

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8.5 Installing the eyepieces
Insert both eyepieces into the tubes of the optical head. (Fig. 9)
FFig. 9ig. 9
8.6 Installing the lamp housing
1. Insert the lamp house and tighten the provided Allen wrench
①. (Fig. 10)
2. Slide the lter holder into the tube in front of the lamp hous-
ing. (Fig.11)
3. Connect the lamp housing connector to the socket placed in
the back of the microscope. (Fig. 12)
• Check for a safe connection.
FFig. 12ig. 12
FFig. 11ig. 11
FFig. 10ig. 10
①