Optika Italy IM-5 Series User manual

Ver. 1.3 2022
Model
IM-5MET
IM-5 Series
INSTRUCTION MANUAL

Page 2
Table of contents
1. Warning 3
2. Safety Information 3
3. Package content 4
4. Unpacking 5
5. Intended use 5
6. Symbols and conventions 5
7. Instrument description 6
8. Assembling 8
8.1 Installing the objectives 8
8.2 Use of nosepiece adapter rings 8
8.3 Removing the stage lock 9
8.4 Installing the stage insert 9
8.5 Installing the eyepieces 10
8.6 Installing the lamp housing 10
8.7 Installing the bulb 11
8.8 Connecting the power cord 11
9. Brightfield observation procedures 13
10. Use of the microscope in Brightfield (BF) 14
10.1 General switch on 14
10.2 Brightness adjustment 14
10.3 SwitchingBrighteldobservation 14
10.4 Adjust the interpupillary distance 15
10.5 Diopter adjustment 15
10.6 Use of eyeshields 15
10.7 Light path selection 16
10.8 Coarse focus tension adjustment 16
10.9 ECO button 16
10.10Centeringtheelddiaphragm(FS) 17
10.11Effectsoftheelddiaphragm 17
10.12 Centering the Aperture Diaphragm (AS) 17
10.13Useofcolorlters 18
11. Use of the microscope in Darkfield (DF) 18
11.1 SwitchingDarkeldobservation 18
12. Use of the microscope in Polarized light (PO) 19
13. Microphotography 20
13.1 Use of C-mount cameras 20
13.2 UseofReexcameras 20
14. Maintenance 21
15. Troubleshooting 22
Equipment disposal 24

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of maintenance. It
is built to high optical and mechanical standards and to withstand daily use. We remind you that this manual contains
important information on safety and maintenance, and that it must therefore be made accessible to the instrument users.
We decline any responsibility deriving from incorrect instrument use uses that does not comply with this manual.
2. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the operation voltage
of the equipment and that the lamp switch is in off position. Users should observe all safety regulations of the region. The
equipment has acquired the CE safety label. However, users have full responsibility to use this equipment safely. Please
follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.

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3. Package content
① Microscopebody
② Eyepieces
③ Stage
④ Objectives
⑤ Lamphousing
⑥ Daylightlter(LBD)
⑦ Analyzer
⑧ Polarizer
⑨ Filterholder
⑩ DICdummyslider
⑪ Powersupply
⑫ Powercord
⑬ Revolvingnosepiece
⑭ Nosepieceadapterrings
⑮ Dustcover
⑯ Stageclips
⑰ Allenwrench
⑱ Metalinsertforstage
⑪
⑩
③
④
⑤
⑬
⑭⑮
⑯⑰
⑥
⑦
⑧⑨
①
②
⑱
⑫

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4. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container and lift the
top half of the container. Take some care to avoid that the optical items (objectives and eyepieces) fall out and get dam-
aged. Using both hands (one around the arm and one around the base), lift the microscope from the container and put it
on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or other
residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
5. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the subject.
6. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.

Page 6
PHOTO/TV
PORT
DIOPTER
ADJUSTMENT
RING
EYEPIECE
LIGHT PATH
SELECTOR
LEVER
COARSE
FOCUS KNOB
FINE FOCUS
KNOB
BF/DF MIRROR
SELECTION LEVER
OBJECTIVE
LAMP HOUSING
MICROSCOPE
BODY
STAGE PLATE
ECO
BUTTON
7. Instrument description

Page 7
TENSION
ADJUSTMENT
COLLAR
BRIGHTNESS
ADJUSTMENT KNOB
MAIN SWITCH
X/Y AXIS KNOBS
APERTURE DIAPHRAGM
FIELD DIAPHRAGM
DIC SLIDER SLOT
NOSEPIECE
Opposite side

Page 8
8. Assembling
FFig. 1ig. 1
FFig. 2ig. 2
FFig. 4ig. 4
FFig. 3ig. 3
②
③
①
8.1 Installing the objectives
1. Turn the coarse focusing knob ① until the nosepiece reach-
es its lowest position. (Fig. 1)
• For a safe transport, the nosepiece is placed in the low-
est position and the tension adjustment collar ②is ad-
justed to the appropriate tension when the microscope
leaves the factory. (Fig. 1)
2. Screw the lowest magnication objective on to the nosepiece
from the right side, then turn the turret clockwise. Install the
other objectives in the same way, following the sequence
from low to high magnication.
• Note: the objectives can also be installed through the
stage opening. (Fig. 2)
• Clean the objectives regularly. In inverted microscopes, the
objectives are very sensitive to dust.
• To prevent dust and contamination from entering the micro-
scope, cover all the unused holes with dust caps ③. (Fig. 3)
• When operating, use the low magnication objective (5x or
10X) to search and focus the specimen, then switch to higher
magnications.
8.2 Use of nosepiece adapter rings
The nosepiece can allocate objectives for brighteld/darkeld
observation (BD).
However, it is possible to install objectives for brighteld using
the adapter rings.
1. Screw the adapter ring into the empty hole of the nosepiece.
(Fig. 4 - 5)

Page 9
2. Screw the objective into the adapter ring. (Fig. 6)
3. Perform the observation as usual.
• The brighteld objective cannot be used for darkeld,
therefore switching the mirror cube selector to “DF” could
bring to a completely dark image.
• Using the adapter rings it could happen an imperfect
parfocality between brighteld and brighteld/darkeld
objectives. This is not a defect.
FFig. 5ig. 5
FFig. 6ig. 6
FFig. 7ig. 7
①
8.3 Removing the stage lock
1. Remove the 4 screws of the metal retaining plate ①, using
the supplied Allen screwdriver. (Fig. 7)
8.4 Installing the stage insert
• Make sure that the plate is horizontal.
1. Install the stage insert in the stage opening. (Fig. 8)
FFig. 8ig. 8

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8.5 Installing the eyepieces
Insert both eyepieces into the tubes of the optical head. (Fig. 9)
FFig. 9ig. 9
8.6 Installing the lamp housing
1. Insert the lamp house and tighten the provided Allen wrench
①. (Fig. 10)
2. Slide the lter holder into the tube in front of the lamp hous-
ing. (Fig.11)
3. Connect the lamp housing connector to the socket placed in
the back of the microscope. (Fig. 12)
• Check for a safe connection.
FFig. 12ig. 12
FFig. 11ig. 11
FFig. 10ig. 10
①

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8.7 Installing the bulb
• Before installing the bulb, set the main switch to “OFF”
and disconnect all electrical cables.
1. Using the provided Allen wrench, release the lamp housing
cover ②. (Fig. 13)
2. Press the spring clips ③ of the lamp holder and insert the
halogen bulb into the holes ④. (Fig. 14)
3. Release the spring clips to lock the bulb in place
• Do NOT touch the bulb with the bare hands: this could
reducethelampefciencyandlifetime.
4. Once installed the bulb, put the lamp housing cover into its
original position and lock the screw ②.
8.8 Connecting the power cord
1. Turn the main switch ① to “OFF” before connecting the
power cord. (Fig. 15)
2. Insert the plug of the provided power supply into the power
socket of the microscope. (Fig. 16)
FFig. 13ig. 13
②
FFig. 14ig. 14
FFig. 15ig. 15
③
①
④
FFig. 16ig. 16

Page 12
FFig. 17ig. 17
3. Plug the power cord into the socket of the power supply. (Fig.
17)
4. Plug the power cord into the mains socket.
• Check for a safe connection.
• Please use the provided power cord.
• Iflostordamaged,pleaserefertoqualiedservice.
• Connect the power cord to a grounded power supply
only.

Page 13
9. Brightfield observation procedures
(Used commands) (Chapter)
Bring on “ON” the main switch
Put a specimen on the stage
Insert 10X objective into the light path
Focus the specimen
Begin observation
Adjust field and aperture diaphragms
Insert desired objective for the observation and focus the
specimen
Adjust interpupillary distance
Adjust diopters
Insert color filters
Adjust light intensity
Move the BF/DF selection lever to “BF” position
Main switch
Nosepiece
Observation head
Diopter adjustment ring
Aperture diaphragm
Field diaphragm
Nosepiece
Filter slider
Coarse and ne focusing knobs
Coarse and ne focusing knobs
10
7
7
7
7
10
10
10
10
10
Light adjustment dial 10
Mechanical stage
X/Y movement knob
10
7
BF/DF selection lever 7
Remove polarizer, analyzer and filters
from the light path
Adjust light intensity Light adjustment dial 10

Page 14
10. Use of the microscope in Brightfield (BF)
10.1 General switch on
To activate the reected light illuminator, put the main switch ①,
located on the right side of the stand, to the position “ON”. (Fig.
18)
FFig. 18ig. 18
FFig. 19ig. 19
FFig. 20ig. 20
①
②
③
FFig. 21ig. 21
④
10.2 Brightness adjustment
1. Turn the brightness adjustment knob ② to increase and
decrease the brightness. (Fig. 19)
2. While rotating the brightness adjustment knob, the LED
indicator on the front panel ③ will turn ON or OFF some
segments. (Fig. 20)
10.3 SwitchingBrighteldobservation
Move the lever ④in “BF” position for brighteld illumination. (Fig.
21)

Page 15
10.4 Adjust the interpupillary distance
Observing with both eyes, hold the two eyepiece prism assem-
blies. Rotate them around their common axis until the elds of
view match.
• The graduation on the interpupillary distance indicator
①, pointed by the spot “.” on the eyepiece holder, shows
the distance between the operator’s eyes. (Fig. 22)
The range of the interpupillary distance is 48-75 mm.
FFig. 23ig. 23
FFig. 24ig. 24
②
FFig. 25ig. 25
FFig. 22ig. 22
①
10.5 Diopter adjustment
• Both eyepieces are provided with diopter adjustment
ring. Put each ring in the “0” position before proceeding
with the adjustment.
1. Look into the right eyepiece with your right eye only, and
focus on the specimen.
2. Look into the left eyepiece with your left eye only. If the
image is not sharp, use the diopter adjustment ring ②to
compensate. (Fig. 23)
• The adjustment range is ±5 diopter. The number indicated
on the adjustment ring graduation should correspond to
the operator’s diopter correction.
10.6 Use of eyeshields
• Use with eyeglasses
Fold rubber eyeshields with both hands. Folded eyeshields avoid
scratching the lenses of eyeglasses. (Fig. 24)
• Use without eyeglasses
Raise eyeshields and observe at the microscope placing eyes
to the shields, avoiding external light to disturb the observation.
(Fig. 25)

Page 16
10.7 Light path selection
• The observation head is equipped with an optical path
selector that allows the light to be distributed to the eyepieces
and to the photo / TV port.
1. Move the selector ① to the left (In) or to the right (Out) to
distribute the light. (Fig. 26)
POSITION LIGHT
IN 100% TV
OUT 50% EYEPIECES - 50% TV
FFig. 26ig. 26
FFig. 27ig. 27
FFig. 28ig. 28
①
②
10.8 Coarse focus tension adjustment
The tension of the coarse focusing knob is factory preset.
1. To modify the tension according to personal’s needs, rotate
the ring ②. (Fig. 27)
• Clockwise rotation increases the tension.
• If the tension is too loose, the revolver could go lower by itself
or the focus easily lost after ne adjustment. In this case,
rotate the knob in order to increase the tension.
10.9 ECO button
1. Press the ECO button (Fig. 28) to activate the “ECO” function.
• Once activated, the system will automatically shut-off after
20 minutes from the activation.
2. To deactivate the function, press the ECO button again.

Page 17
10.10Centeringtheelddiaphragm(FS)
1. Move the selector on the reected light illuminator in the
position “BF”. (Fig. 21)
2. Place the specimen on the stage, insert 10x objective into the
light path and focus.
3. Completely pull out the eld diaphragm lever ① to fully close
the diaphragm. (Fig. 29)
4. Using the provided Allen wrench use the two centering
screws ② to bring the bright spot in the centre of the eld of
view. (Fig. 29)
5. Gradually open the diaphragm. The diaphragm is centered
when the diaphragm image is symmetrical to the eld of
view. (Fig. 30)
6. In normal use, open the diaphragm until it circumscribes the
eld of view.
FFig. 30ig. 30
FFig. 31ig. 31
FFig. 29ig. 29
①
②
FFig. 40ig. 40
FFig. 32ig. 32
APERTURE
DIAPHRAGM
FIELD OF VIEW
30-20%
70-80%
③
④
10.12 Centering the Aperture Diaphragm (AS)
1. Move the selector on the reected light illuminator in the
position “BF”. (Fig. 21)
2. Completely pull out the aperture diaphragm lever ③ to fully
close the diaphragm. (Fig. 31)
3. Remove one eyepiece.
4. While looking into the empty eyepiece sleeve, use the
provided Allen wrench into the centering screws ④to bring
the bright spot in the centre of the eld of view. (Fig. 31)
5. The illuminator is centered when the diaphragm image is
symmetrical to the eld of view.
• The Numerical Aperture (N.A.) value of the aperture
diaphragm affects the image contrast. Increasing or reducing
this value one can vary resolution, contrast and depth of
focus of the image.
• With low contrast specimens move the diaphragm lever to
about 70%-80% of the objective’s N.A. If necessary, remove
on eyepiece and, looking into empty sleeve, adjust the AS
lever in order to obtain an image like the one in Fig. 32.
10.11Effectsoftheelddiaphragm
Field diaphragm adjusts the illuminated area to obtain a high
contrast image.
Set the diaphragm according to the objective in use until it
circumscribes the eld of view, in order to eliminate unnecessary
light to eyepieces. (Fig. 30)

Page 18
10.13Useofcolorlters
1. Insert the color lter in the slot of the lter holder. (Fig. 33)
2. Move the slider into one of the three available position until it
clicks into the right position.
FILTER APPLICATION
Blue Converts the source colour temperature to
daylight
Frosted white Reduces lighting irregularities by creating a
homogeneous eld of view
FFig. 33ig. 33
11. Use of the microscope in Darkfield (DF)
11.1 SwitchingDarkeldobservation
• Themicroscopeallowstoworkinbrighteld(BF)ordarkeld(DF).
• Forobservationindarkelditisnecessarytousespecicobjectivescalled“BD”.
1. Move the lever in “DF” position for darkeld illumination. (Fig. 21)
• Darkeldobjectivesalsoallowobservationinbrighteld.

Page 19
12. Use of the microscope in Polarized light (PO)
The performance of polarizer may deteriorate
whenithasbeenexposedtolightforalongpe-
riod (about continuous 2000 hours). If this hap-
pens, replace the polarizer.
1. Move the lever in “BF” position for brighteld illumination.
(Fig. 21)
• Polarized light observation cannot be performed when
theBF/DFselectorisin“DF”position.
2. Insert the polarizer “PO” with the inscription facing the user.
3. Move the polarizer “PO” into the light path until the slider
clicks into its position. (Fig. 34)
FFig. 35ig. 35
FFig. 36ig. 36
FFig. 34ig. 34
①
4. Insert analyzer “AN” with the inscription facing up.
5. Move the rotatable analyzer “AN” into the light path until the
slider clicks into its position. (Fig. 35)
6. Put a at mirror on the stage and get into approximate focus
the mirror surface.
7. While observing into the eyepieces, rotate the scale of the
analyzer ①until you achieve the darkest image. (Fig. 36)
• Now the light extinction (so-called “crossed Nicol position”)
is achieved.
8. Put a specimen on the stage, focus the specimen and begin
observation.

Page 20
13. Microphotography
13.1 Use of C-mount cameras
1. Loosen the clamping screw ① on the trinocular port and
remove the dust cap ②. (Fig. 37) ②
FFig. 37ig. 37
FFig. 38ig. 38
FFig. 39ig. 39
④
①
②
③
④
③
①
2. Screw the C-mount adapter ③to the camera ④ and insert
the round dovetail of the C-mount into the empty hole of the
trinocular port, then tighten the clamping screw ①. (Fig. 38)
13.2 UseofReexcameras
1. Insert the Reex adapter ①into the relay tube to the micro-
scope ②.
2. Screw the “T2” ring ③ (not provided) to the reex adapter.
3. Connect the Reex camera ④to the “T2” ring just installed
(Fig. 39).
4. Mount the other end of the relay tube ②into the empty hole
of the trinocular port, then tighten the clamping screw. (Fig.
37)
• “T2” ring is not provided along with the microscope, but is
commercially available.
• While shooting dark specimens, darken eyepieces and view-
nder with a dark cloth to minimize the diffused light.
• To calculate the magnication of the camera: objective magni-
cation * camera magnication * lens magnication.
• If using an SLR camera, mirror movement may cause the
camera to vibrate.
• Wesuggestliftingthemirror,usinglongexposuretimes
and a remote cord.