OPTIKA MICROSCOPES B-290 Series User manual

Model
B-290 series (B-292 / B-292PLI / B-293 / B-293PLI)
B-290LD series (B-292LD1.50 / B-292LD1 / B-293LD1.50 / B-293LD1)
B-290TB series (B-290TB)
B-290 Series
INSTRUCTION MANUAL
Ver. 3.0 2019

Page 2
1. Warning 3
2. Symbols and conventions 3
3. Safety Information 3
4. Intended use 3
5. Overview 4
5.1 B-292 / B-292PLI / B-293 / B-293PL 4
5.2 B-292LD1.50 - B-292LD1 - B-293LD1.50 - B-293LD1 5
5.3 B-290TB 6
6. Unpacking 7
7. Assembling 7
7.1 B-292 / B-292PLI / B-293 / B-293PLI 7
7.2 B-292LD1 / B-292LD1.50 / B-293LD1 / B-293LD1.50 8
7.3 B-290TB 9
7.4 Assembling the microscope 10
7.4.1 B-292 / B-292PLI / B-293 / B-293PLI 10
7.4.2 B-292LD1/B-292LD1.50/B-293LD1/B-2932LD1.50 11
7.4.3 B-290TB 12
7.5 Polarizing set (optional) 14
8. Use of the microscope 15
8.1 Switching on the microscope 15
8.2 Light intensity adjustment 15
8.3 Coarse focus tension adjustment 15
8.4 Stage 15
8.5 Adjust the interpupillary distance 16
8.6 Diopter adjustment 16
8.7 Use of oil immersion objective 16
8.8 Condenser centering 17
8.9 Aperture diaphragm 17
8.10 Useofuorescence 18
8.11 Use of the polarizer (optional) 18
9. Microphotography 19
9.1 Cameras with projection lens 19
9.2 Reexcamera 19
10. Troubleshooting 20
11. Maintenance 22
Equipment disposal 23
Summary

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of
maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you
that this manual contains important information on safety and maintenance, and that it must therefore be made
accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses
that does not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibility
to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
4. Intended use
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.

Page 4
5. Overview
5.1 B-292 / B-292PLI / B-293 / B-293PL
FINE FOCUS KNOB
PHOTO TUBE (ONLY FOR
TRINOCULAR HEAD)
INTENSITY ADJUSTMENT
DIAL
STAGE
OBJECTIVES
COARSE FOCUS KNOB
CONDENSER
X/Y MOVEMENT
KNOBS
SLIDE HOLDER
CONDENSER
HEIGHT
ADJUSTMENT
KNOB
CONDENSER
CENTERING SCREWS
OBSERVATION HEAD
-) BINOCULAR (B-292 / B-292PLI)
-) TRINOCULAR (B-293 / B-293PLI)
EYEPIECES

Page 5
5.2 B-292LD1.50 - B-292LD1 - B-293LD1.50 - B-293LD1
INTENSITY ADJUSTMENT
DIAL
COARSE FOCUS KNOB
FINE FOCUS KNOB
CONDENSER
SLIDE HOLDER
CONDENSER
HEIGHT
ADJUSTMENT
KNOB
STAGE
OBJECTIVES
X/Y MOVEMENT
KNOBS
CONDENSER
CENTERING SCREWS
EYEPIECES
PHOTO TUBE (ONLY FOR
TRINOCULAR HEAD)
OBSERVATION HEAD
-) BINOCULAR
(B-292LD1 / B-292LD1.50)
-) TRINOCULAR
(B-293LD1 / B-293LD1.50)
FLUORESCENCE
ATTACHMENT

Page 6
5.3 B-290TB
INTENSITY ADJUSTMENT
DIAL
COARSE FOCUS
KNOB
FINE FOCUS KNOB
STAGE
X/Y MOVEMENT
KNOBS
SLIDE HOLDER OBJECTIVES
DIGITAL HEAD
TABLET
EYEPIECES
CONDENSER
HEIGHT
ADJUSTMENT
KNOB
CONDENSER
CENTERING SCREWS

Page 7
6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or
other residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
7. Assembling
Once opened the box, the microscope parts are the following:
7.1 B-292 / B-292PLI / B-293 / B-293PLI
① Frame
② Observation head
binocular (B-292 / B-292PLI)
trinocular (B-293 / B-293PLI)
③ Photo tube (only B-293 series)
④ Eyepieces
⑤ Objectives (4X / 10X / 40X / 100X)
⑥ Dust cover
⑦ Green lter
⑧ Power supply
⑨ Immersion oil
⑩ Tension adjustment tool
③
④
⑤
⑥
⑦
⑧
①
②
⑨
⑩

Page 8
7.2 B-292LD1 / B-292LD1.50 / B-293LD1 / B-293LD1.50
① Frame
② Observation head
binocular (B-292LD1 / B-292LD1.50)
trinocular (B-293 / B-293PLI)
③ Photo tube (only B-293 series)
④ Eyepieces
⑤ Objectives
10X/20X/40X/50X: B-292LD1.50 - B-293LD1.50
10X/20X/40X/100X(dry): B-292LD1 - B-293LD1
⑥ Dust cover
⑦ Fluorescence attachment
⑧ Power supply
⑨ Tension adjustment tool
③
④
⑤
⑥
⑦
⑧
①
②
⑨

Page 9
7.3 B-290TB
① Frame
② Digital observation head
③ Eyepieces
④ Objectives (4X / 10X / 40X / 100X)
⑤ Dust cover
⑥ Green lter
⑦ Immersion oil
⑧ Power supply
⑨ Tension adjustment tool
⑩ Tablet power supply
⑪ OTG cable
⑫ USB cable
⑬ Touch pen
⑭ Tablet + keyboard
②
⑩
⑪
⑫
⑬
⑭
③
④
⑤
⑥
⑦
⑧
①
⑨

Page 10
7.4 Assembling the microscope
7.4.1 B-292 / B-292PLI / B-293 / B-293PLI
1. Remove the dust cap from the microscope frame
and from the bottom of the observation head.
2. Insert the optical head above the stand and
tighten the screw. (Fig. 1)
• Hold the head with one hand during the lo-
cking in order to avoid that the head falls.
3. Insert both eyepieces into the tubes of the optical
head. (Fig. 2)
4. Insert the power supply jack in the socket placed
at the rear side of the microscope. (Fig. 3)
Only for trinocular head
Unscrew the protection cap mounted on the photo
port and screw the photo tube. (Fig. 4)
Fig. 1
Fig. 2
Fig. 3
Fig. 4

Page 11
7.4.2 B-292LD1/B-292LD1.50/B-293LD1/B-2932LD1.50
Fig. 5
Fig. 6
Fig. 8
Fig. 7
1. Insert the uorescence attachment above
the frame and tighten the locking screw.
(Fig. 5)
2. Insert the cable in the socket placed at
the rear side of the microscope. (Fig. 6)
3. Insert the optical head above the stand and
tighten the screw. (Fig. 7)
• Hold the head with one hand during the
locking in order to avoid that the head
falls.
4. Insert both eyepieces into the tubes of the
optical head. (Fig. 8)

Page 12
5. Insert the power supply jack in the socket placed
at the rear side of the microscope. (Fig. 3)
Only for trinocular head
Unscrew the protection cap mounted on the photo
port and screw the photo tube. (Fig. 4)
7.4.3 B-290TB
1. Remove the dust cap from the microscope frame
and from the bottom of the observation head.
2. Insert the optical head above the stand and
tighten the screw. (Fig. 11)
• Hold the head with one hand during the lo-
cking in order to avoid that the head falls.
3. Insert both eyepieces into the tubes of the optical
head. (Fig. 12)
Fig. 9
Fig. 10
Fig. 11
Fig. 12

Page 13
4. Fix the rotating part of the junction using
the black wing-nut ①. (Fig. 13)
5. Then hook the Tablet PC onto the 4
screws of the junction and pull toward
down to rmly lock the Tablet PC in the
holder. (Fig. 14)
• To unlock the Tablet PC proceed with
the opposite operation: push toward
up and remove it from the holder.
6. Plug one side of the cable named CA-
MERA CONNECTION (USB + OTG) ②
to the digital head and the other side to
the Tablet PC. (Fig. 15).
7. Plug the cable named POWER SUPPLY
CONNECTION to the Tablet PC for bat-
tery recharge.
Fig. 13
Fig. 14
①
Your Tablet’s been set with the Rotation function disabled: this prevents any ipping of the Live View in order to
get a continuous and as large as possible view of your slide also when the Tablet is removed from the holder. To
enable this function again is very easy: you can activate the Rotation by swiping the screen on his bottom right
side and selecting Settings + Screen. Anyway, it’s not suggested to activate the function when the camera is in
Live View mode as it may give troubles when the camera runs at high resolutions.
The Tablet PC can be xed to a junction.
Fig. 15
②

Page 14
7.5 Polarizing set (optional)
1. Place the polarizer on the light exit ①at the base
of the microscope. (Fig. 16)
2. Loosen the head xing knob ② and remove the
head from the microscope frame. (Fig. 17)
3. Insert the analyzer into the hole inside the frame
③. (Fig. 18)
4. Put back the head into its original position and
lock the xing knob.
Fig. 18
③
Fig. 16
①
Fig. 17
②

Page 15
8. Use of the microscope
8.1 Switching on the microscope
Operate on the main switch ① placed in the rear side
of the microscope, moving the selector on “I” (Fig. 19)
8.2 Light intensity adjustment
Operate on the light intensity dial to increase or de-
crease the illumination intensity. (Fig. 20)
8.3 Coarse focus tension adjustment
• Adjust the tension using the provided tool.
The coarse knob tension is pre-setted in the factory.
To modify the tension according to personal’s needs,
rotate the ring ③ using the provided tool (Fig. 21).
Clockwise rotation increases the tension. If the ten-
sion is too loose, the stage could go lower by itself
or the focus easily lost after ne adjustment. In this
case, rotate the knob in order to increase the tension.
8.4 Stage
Stage accepts standard slides 26 x 76 mm, thickness
1,2 mm with coverslide 0,17mm. (Fig. 22)
1. Open the spring arm of the slide holder ② and
place the slide from the front on the stage.
2. Gently release the spring arm of the slide holder.
• A sudden release of the spring arm could cau-
se the falling of the slide.
Fig. 7
Fig. 19
Fig. 20
Fig. 21
Fig. 22
②
①

Page 16
8.5 Adjust the interpupillary distance
Hold the right and left parts of the observation head
using both hands and adjust the interpupillary distan-
ce by turning the two parts until one circle of light can
be seen. (Fig. 23)
• The graduation on the interpupillary distance in-
dicator ①, pointed by the spot “.” on the eyepiece
holder, shows the distance between the opera-
tor’s eyes.
The range of the interpupillary distance is 48- 75 mm.
8.6 Diopter adjustment
This operation can be done only on binocular
models.
1. Look into the right eyepiece with your right eye
only, and focus on the specimen.
2. Look into the left eyepiece with your left eye
only. If the image is not sharp, use the dioptric
adjustment ring ②to compensate. (Fig. 24)
• The adjustment range is ±5 diopter. The num-
ber indicated on the adjustment ring gradua-
tion should correspond to the operator’s dio-
ptric correction.
8.7 Use of oil immersion objective
AllmodelsexceptLDseries
1. Focus the specimen with a low power objective.
2. Lower the stage.
3. Put a drop of oil (provided) on the area of the
specimen to be observed. (Fig. 25)
• Make sure that there are no oil bubbles. Air
bubbles in the oil damage the image quality.
• To check for bubbles: remove an eyepiece, ful-
ly open the aperture diaphragm and observe the
objective exit pupil. (The pupil must be circular
and bright).
• To remove the bubbles, gently move the nose-
piece to the right and left to move the immersion
objective a few times and allow the air bubbles
to move.
4. Insert immersion objective.
5. Return the stage to the upper focusing point and
obtain an optimal focus using the ne focus knob.
6. After use, gently remove the oil with a soft paper
towel or a lightly moistened optic paper with a
mixture of ethyl ether (70%) and absolute ethyl
alcohol (30%).
• The immersion oil, if not immediately cleaned,
could crystallize creating a glass-like layer.
In this situation the observation of the speci-
menwouldbedi󰀩cult(evennotimpossible)
due to the presence of an additional thickness
on the objective.
Fig. 23
Fig. 25
①
Fig. 24
②

Page 17
8.8 Condenser centering
The condenser is installed and pre-centered in the
factory.
To remove the condenser use an Allen wrench 1.5
mm and operate on the xing knob placed on the
right side of the condenser holder.
Should a new centering is needed, operate in this
way:
1. Insert 4x objective in the light path (in case 4x is
not available use the lower magnication availa-
ble).
2. Focus the specimen.
3. Close the aperture diaphragm using the ring ①,
moving the ring to the value “4” related to the 4x
objective. (Fig. 26)
4. Raise the condenser to the upper limit using the
height adjustment knob ② placed on the left side
of the condenser holder.
5. Center the condenser using the centering screws
③ until the eld of view is evenly illuminated (in
the eld of view no dark and bright areas must be
noticed).
6. Fully open the diaphragm.
8.9 Aperture diaphragm
The Numerical Aperture (N.A.) value of the aperture
diaphragm a󰀨ects the image contrast. Increasing or
reducing this value one can vary resolution, contrast
and depth of focus of the image.
Move the diaphragm ring ① (Fig. 26) on the value
corresponding to the objective in use. In this case the
optimal setting of the condenser is achieved.
It is possible, however, move the ring to lower or hi-
gher values to adapt the observation to personal pre-
ferences.
• With low contrast specimens set the numerical
aperture to about 70%-80% of the objective’s
N.A. If necessary, remove on eyepiece and, lo-
oking into empty sleeve, adjust the condenser’s
diaphragm in order to obtain an image like the
one in Fig. 27.
Fig. 27
IRIS DIAPHRAGM
FIELD OF VIEW
30-20%
70-80%
Fig. 26
①
②
③

Page 18
8.10 Useofuorescence
Operate on the main switch placed in the rear
side of the microscope. Setting on “I” turns on
transmitted light, setting on “II” turns on uore-
scence.
Setting on “O” turns o󰀨 the microscope. (Fig.
28)
Move the lter selector in the “B” position (Fig.
29) to insert the uorescence lter in the light
path. Move the selector in the center to work
with brighteld transmitted light.
Unlike a mercury lamp system, B-290LD LED
illumination doesn’t need any power-up time
for heating, and can be used immediately after
switching on. Also, the LED source is pre-alig-
ned in factory and doesn’t need any alignment
operation.
Focus on your sample, and adjust the light
intensity as needed through the brightness
adjustment knob. In order to improve the
darkness of the background (thus improving
contrast), it is strongly suggested to put the put
a dark cover on the light exit at the base of the
microscope.
FILTER
NAME EXCITATION FILTER DICHROIC MIRROR BARRIER FILTER APPLICATIONS
B 460 - 490 nm 505 nm 515LP nm • FITC: fluorescent antibodies • Achridine orange DNA - RNA
• Auramine
8.11 Use of the polarizer (optional)
1. Remove the specimen from the stage.
2. Looking inside the eyepieces, rotate the po-
larizer until the darkest position is achieved.
3. Once the dark is achieved (“extinction” or
“Crossed Nicol” position) it is possible to
begin the observation.
Fig. 28
Fig. 29

Page 19
9. Microphotography
9.1 Cameras with projection lens
1. Remove dust caps from camera and projection
lens.
2. Screw the projection lens to camera thread. (Fig.
30)
3. Insert the projection lens into the photo tube.
(Fig. 31)
9.2 Reexcamera
1. Screw the “T2” ring (not provided) at the end of the
projection lens (M-173), then install everything to
the reex camera. (Fig. 32)
2. Insert the projection lens into the photo tube.
(Fig. 33)
Fig. 30
Fig. 31
M1
M1) Screw the Reex adapter to the “T2” ring (provided with the
Fig. 32
M2
M3) Properly install the Reex camera’s adapter
on the trinocular port
Fig. 33

Page 20
10. Troubleshooting
Review the information in the table below to troubleshoot operating problems.
PROBLEM CAUSE SOLUTION
I. Optical Section:
LED operates, but eld of view re-
mains dark. Power supply is unplugged. Connect
Brightness is too low Set brightness to a proper level
Fluorescence filter is not suitable
for the specimen Use a suitable filter
Dirt or dust is visible in the eld of
view. Dirt/dust on the specimen Clean the specimen
Dirt/dust on the eyepieces Clean the eyepieces
Image looks double Aperture diaphragm is stopped
down too far Open aperture diaphragm
Visibility is poor.
• Image is not good.
• Contrast is poor.
• Details are indistinct.
• Image glares
Revolving nosepiece is in an
incorrect position Move the nosepiece to a click stop
Aperture diaphragm is too closed
or to open Adjust aperture diaphragm
Dust or dirt on lenses (condenser,
objectives, eyepieces and slide) Clean thoroughly
For transmitted light observation,
the coverglass thickness must not
exceed 0.17mm
Use a coverglass with thickness 0.17mm
Focus is not even Slide holder is not flat. Move the specimen
to a flat position
One side of the image is out of focus. The nosepiece is not in the center
of the light path Turn the nosepiece to a click stop
The specimen is out of place
(tilted) Place the specimen at on the stage.
The optical performance of the
sample cover glass is poor Use a cover glass of better quality
II. Mechanical Section:
The coarse focus knob is hard to
turn. The tension adjustment collar is too tight Loosen the tension adjustment collar
The focus is unstable. The tension adjustment collar is
too loose Tighten the tension adjustment collar
III. Electric section
The LED doesn’t turn on. No power supply Check the power cord connection
The brightness is not enough The brightness adjustment is low Adjust the brightness
The light blinks The power cord is poorly con-
nected Check the power cord
IV. Observation tube
Field of view of one eye does not
match that of the other. Interpupillary distance is incorrect. Adjust interpupillary distance.
Incorrect diopter adjustment.Adjust diopter.
Your view is not accustomed to
microscope observation.
Upon looking into eyepieces, try looking at
overall field before concentrating on speci-
men range. You may also find it helpful to
look up and into distance for a moment
before looking back into microscope.