Optika IM Series User manual

Ver. 5.0 2019
Model
IM-3
IM-3F
IM-3FL4
IM-3LD
IM Series
INSTRUCTION MANUAL

Page 2
Table of Contents
1. Warning 3
2. Symbols and conventions 3
3. Safety Information 3
4. Intended use 3
5. Overview 4
5.1 IM-3 4
5.2 IM-3F / IM-3FL4 5
5.3 IM-3LD 6
6. Unpacking 7
7. Assembling 7
7.1 IM-3 7
7.2 IM-3F / IM-3FL4 8
7.3 IM-3FLD 9
7.4 Installation procedure 10
7.4.1 Installing the objectives 10
7.4.2 Installing stage extension or mechanical stage 10
7.4.3 Installing the stage insert 11
7.4.4 Installing the eyepieces 11
7.4.5 Installing condenser and LED housing 11
7.4.6Installingcolorlters 12
7.4.7 Connecting the power supply 12
7.4.8Installinguorescence 13
8. Brighteldobservationprocedures 16
9. Using the microscope (BF or PH) 17
9.1 Turning on the microscope 17
9.2 Adjusting the light intensity 17
9.3 Adjusting the coarse focus tension 17
9.4 Diopter adjustment 17
9.5 Adjusting interpupillary distance 18
9.6 Use of eyeshields 18
9.7 Selecting the light path 18
9.8 Mechanical stage and sample holders 19
9.9 Aperture diaphragm 20
9.10 Usingcolorlters 20
9.11 Phase contrast 20
9.11.1 Phase contrast slider 21
9.11.2 Centering the phase ring 21
10. Fluorescence observation procedures (IM-3F / IM-3FL4) 22
11. Fluorescence observation procedures (IM-3LD) 22
12. Using the microscope (FL) 23
12.1 Centering the mercury bulb 23
12.2 Centeringtheelddiaphragm 25
12.3 Using the microscope 25
12.3.1Switchinguorescenceltercubes 25
12.3.2 Filter holder / Shutter 27
12.3.3InsertingaNDlter 27
12.3.4Usingthelterslider 27
13. Simultaneous observation in Phase Contrast + HBO Fluorescence 28
14. Microphotography 29
14.1 Use of C-mount cameras 29
14.2 UseofReexcamera 29
15. Maintenance 30
16. Troubleshooting 31
Equipment disposal 33

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of mainte-
nance. It is built to high optical and mechanical standards and to withstand daily use. We remind you that this
manual contains important information on safety and maintenance, and that it must therefore be made accessi-
ble to the instrument users. We decline any responsibility deriving from incorrect instrument use uses that does
not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the ope-
ration voltage of the equipment and that the lamp switch is in off position. Users should observe all safety regu-
lations of the region. The equipment has acquired the CE safety label. However, users have full responsibility to
use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure safe
operation of the unit.
4. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the
subject.

Page 4
5. Overview
FILTER HOLDER
PHASE
CONTRAST SLIDER
PHOTO/TV
PORT
DIOPTRIC
ADJUSTMENT
RING
EYEPIECE
LED
HOUSING
CONDENSER
COARSE
FOCUS KNOB
FINE FOCUS KNOB
TENSION
ADJUSTMENT
COLLAR
OBJECTIVE
STAGE
MICROSCOPE
BODY
LIGHT PATH
SELECTOR
LEVER
GLASS STAGE
INSERT
5.1 IM-3

Page 5
5.2 IM-3F / IM-3FL4
PHOTO/TV
PORT
DIOPTRIC
ADJUSTMENT
RING
EYEPIECE
PHASE CONTRAST SLIDER
(OPTIONAL FOR IM-3FL4)
LED
HOUSING
CONDENSER
FILTER HOLDER
COARSE
FOCUS KNOB
FINE FOCUS KNOB
FLUORESCENCE
POWER SUPPLY
TENSION
ADJUSTMENT
COLLAR
OBJECTIVE
STAGE
MICROSCOPE
BODY
LIGHT PATH
SELECTOR
LEVER
GLASS STAGE INSERT
FLUORESCENCE
LAMP HOUSING
UV SHIELD

Page 6
5.3 IM-3LD
DIOPTRIC
ADJUSTMENT
RING
EYEPIECE
PHASE
CONTRAST
SLIDER
LED
HOUSING
FILTER HOLDER
COARSE
FOCUS KNOB
FINE FOCUS KNOB
TENSION
ADJUSTMENT COLLAR
OBJECTIVE
STAGE
MICROSCOPE
BODY
LIGHT PATH
SELECTOR
LEVER
GLASS INSERT
STAGE
ANTI-GLOW CAP
CONDENSER
PHOTO/TV
PORT

Page 7
6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
7. Assembling
7.1 IM-3
Once you open the IM-3 box, these are the microscope’s components:
① Microscope body
② Condenser
③ Filter holder
④ LED illuminator
⑤ Phase contrast slider
⑥ Eyepieces
⑦ Centering telescope
⑧ Metal insert for stage
⑨ Glass insert for stage
⑩ Objectives
⑪ Power supply
⑫ Green lter IF550
⑪
⑫⑩
③④
⑤
⑥
⑦⑧ ⑨
①②

Page 8
① Microscope body
② Condenser
③ LED illuminator
④ Fluorescence power supply + cable
⑤ Fluorescence power cable
⑥ Filter holder
⑦ Metal insert for stage
⑧ Glass insert for stage
⑨ Objectives
⑩ Eyepieces
⑪ Fluorescence lters
⑫ Green lter IF550
⑬ UV shield
⑭ Fluorescence illuminator
⑮ Mercury lamp house
⑯ Microscope power supply
⑰ Phase contrast slider
Provided with IM-3F
Optional with IM-3FL4
①
②
⑪
⑫
⑬⑭
⑮
⑩
③
④
⑤⑥
⑦ ⑧
⑨
7.2 IM-3F / IM-3FL4
Once you open the IM-3F / IM-3FL4 box, these are the microscope’s components:
⑯
⑰

Page 9
7.3 IM-3FLD
Once you open the IM-3LD box, these are the microscope’s components:
① Microscope body
② Condenser
③ LED illuminator
④ Filter protection covers
⑤ Anti-glow cap
⑥ Phase contrast slider
⑦ Glass insert for stage
⑧ Fluorescence lters
⑨ Objectives
⑩ Eyepieces
⑪ Power supply
①
②
⑪
⑩
③
④
⑤
⑥⑦
⑧
⑨

Page 10
7.4 Installation procedure
7.4.1 Installing the objectives
1. Turn the coarse focusing knob ① until the nose-
piece reaches its lowest position.
• For a safe transport, the nosepiece is placed
in the lowest position and the tension adjust-
ment collar ②is adjusted to the appropriate
tension when the microscope leaves the fac-
tory. (Fig.1)
2. Screw the lowest magnication objective on the
nosepiece from the right side, then turn the no-
sepiece clockwise. Mount the other objectives in
the same way, following the sequence from low
to high.
• Note: the objectives can also be installed
through the stage opening. (Fig. 2)
• Clean the objectives regularly. In inverted micro-
scopes, the objectives are very sensitive to dust.
• To prevent dust and contamination from entering
the microscope, cover all the unused holes with
dust caps ③. (Fig. 3)
• When operating, use the low power objective
(10X) to search and focus the specimen, then
switch to higher magnications.
• When switching between objectives, slowly turn
the nosepiece until it clicks. The click means that
the objective is in the right position, in the center
of the light path.
Fig. 1
①
②
Fig. 2
Fig. 3
③
7.4.2 Installing stage extension or mechanical stage
• Stage extension and mechanical stage are
optional accessories for all the models.
Stage extension can be installed on either side of the
stage to enlarge the working surface.
Mechanical stage can only be installed on the right
side of the stage.
1. Installing the units: screw the bolts in the xing
holes of the attachments, then mount the unit
from below the stage. (Fig. 4)
Fig. 4