PerkinElmer FL 6500 User manual

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PerkinElmer, 710 Bridgeport Avenue,
Shelton, CT 06484-4794, U.S.A
Produced in the USA.
FL 6500/8500 Low Temperature Cell Holder Installation
Instructions
This instruction sheet describes the installation of this accessory which is used with the FL 6500/8500
Fluorescence Spectrometer.
NOTE:
Read these instructions before you install this accessory.
Contacting PerkinElmer
Supplies, replacement parts, and accessories can be ordered directly from PerkinElmer, using the part
numbers.
See our website:
http://perkinelmer.com
PerkinElmer's catalog service offers a full selection of high-quality supplies.
To place an order for supplies and many replacement parts, request a free catalog, or ask for information:
If you are located within the U.S., call toll free 1-800-762-4000, 8 a.m. to 8 p.m. EST. Your order will be
shipped promptly, usually within 24 hours.
If you are located outside of the U.S., call your local PerkinElmer sales or service office.
Features
• Easy to install
• Easy to use
• Used for measurement of fluorescence and phosphorescence at a liquid nitrogen temperature
Figure 1 Low Temperature Cell Holder (P/N:N4201045)

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Dimensions and Specifications
Physical Characteristic Specifications
Cooling System Liquid nitrogen
Cooling Temperature 77K (-196℃)
Liquid Nitrogen Reservoir (L) 0.84
LT Cell Holder Dimensions (mm) 197(W) x 275(D) x 177.5(H)
Weight (Kg) 2.48
Accessory Cover Dimensions (mm) 180(W) x 90(D) x 83.5(H)
Cover Weight (Kg) 0.22
Configuration of the Low Temperature Cell Holder
Figure 2 Low Temperature Cell Holder
Figure 3 Accessory Cover

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Figure 4 Cover for Alignment Figure 5 Teflon Rod
NOTE:
NMR Tube for experiment is required to use this accessory. The NMR Tube is not included in the
accessory configuration and should be purchased separately. We recommend a quartz material tube of
5mm outer diameter, 3.4mm inner diameter and 7 inches length.
Installation
1. Prepare the FL 6500/8500 Fluorescence Spectrometer to install this accessory.
2. Connect the instrument power cord and the communication cable.
3. Loosen the accessory fixing bolt and remove the current sample accessory.
Figure 6Loosening the accessory fixing bolt
4. Pull out the cell holder by hand.
Figure 7Pulling out the cell holder

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5. Remove the current front cover plate.
Figure 8Front Cover Plate
6. After checking the pogo pin position in the sample compartment, place the Low Temperature Cell
Holder to be fit into the pogo pin well.
Figure 9Installing the accessory
7. With the bolts secure the front cover plate of the Low Temperature Cell Holder.
Figure 10 Tightening the bolts
8. Tighten the accessory fixing bolt again.
Figure 11 Tightening the accessory fixing bolt

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9. With opening the lid door, then close the sample compartment lid, and then mount the accessory
cover.
Figure 12 Mounting the accessory cover
10.Connect the gas flow. (Go to page 9 “Purging the instrument.”)
Figure 13 Purge gas flow connection
11.Switch on the instrument.

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Alignment
NOTE: When the Low Temperature Cell Holder is installed for the first time and the unexpected peak is shown,
you must align to achieve the optimal spectrum. This must be performed at room temperature.
1. Loosen the six M4 screws, and then remove the top cover.
2. Loosen (but do not remove) the four screws (see the red circle in the following photo).
3. Insert the alignment cover into the hole of the screws.
4. Close the sample compartment lid and open the lid door and then mount the accessory cover.

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5. Prepare a solution of a suitable fluorescent sample.
For example, 10 µM Rhodamine 6G in Ethanol.
6. Fill one of the sample tubes to a depth of 35 mm with the sample.
7. Wipe the outside of the tube to dry it, and then insert the tube gently down and close the sample
cover.
8. In Spectrum FL, start the Spectra Scan mode and enter the following parameters:
Scan Type: Emission Scan
Scan Mode: Single Scan in Intensity
Luminescence Mode: Fluorescence
Excitation wavelength: 490 nm
Emission wavelength: 510 nm ~ 700 nm
Initial Dark: True
Excitation slit/Emission slit: 10 nm / 10 nm
NOTE:
These parameters are only for using Rhodamine 6G as the sample. If another sample is used, then
the excitation and emission wavelength will have to be set accordingly.
9. Save the method, and then click Run icon.

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10. When the measurement is finished, check if the spectrum shape looks similar with the optimal
spectrum of #15. If you’ve got the good spectrum, you can skip steps 11 to14.
11. Remove the accessory cover and open the sample compartment lid carefully.
12. Turn the alignment cover slightly to change the angle of the inner copper block.
13. After opening the lid door, close the sample compartment lid, and then mount the accessory
cover again.
14. Measure the sample again. Repeat the procedure until a good Spectrum is obtained.

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15. The following spectrum shows the optimal angle of separation between fluorescence and
Rayleigh light.
NOTE: If fluorescence and Rayleigh are overlapping, the angle needs to be adjusted. (see step 12)
Examples of erroneously measured spectra
16. After achieving the optimal angle, remove the sample tube and the alignment cover.
17. Tighten the four securing screws.
CAUTION
ATTENTION
While tightening the screws, be careful since the position of the sample
tube can be shifted.
Lors du serrage des vis, faites attention car la position du tube à
échantillon peut être décalée.
18. Place the top cover of the accessory, and secure it using the four screws removed previously.
Purging the instrument
Before measurement of the liquid nitrogen temperatures can be performed, the instrument must
be purged with dry gas, for example helium or nitrogen. This gas must be free of carbon dioxide
and moisture.
The purpose of purging is to avoid the build-up of ice on optical surfaces inside the instrument, in
the sample area and on the sample tube itself. A gas pressure of 0.025 MPa is normally sufficient.

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The gas flow must be connected to the one touch fitting on the front cover plate as shown below.
Tubing O.D size is 4 Ø.
Filling the accessory with liquid nitrogen
WARNING
AVERTISSEMENT
During filling of the liquid nitrogen tank, protective gloves must be
worn, and care must be taken not to touch the metal surfaces of
the accessory with unprotected hands.
Lors du remplissage du réservoir d'azote liquide, il faut porter des
gants de protection et veiller à ne pas toucher les surfaces
métalliques de l'accessoire avec des mains non protégées.
WARNING
AVERTISSEMENT
Due to the danger of asphyxiation please make sure that the area
in which the instrument/accessory and liquid nitrogen vessel are
located is well ventilated.
En raison du risque d'asphyxie, veuillez vous assurer que la zone
dans laquelle se trouvent l'instrument/l'accessoire et le récipient
d'azote liquide est bien ventilée.