PerkinElmer 200A Series User manual

SERIES 200A
FLUORESCENCE DETECTOR
User’s Guide
LIQUID CHROMATOGRAPH
Y

Release History
Part Number Release Publication Date
09936761 B April 2006
Any comments about the documentation for this product should be addressed to:
PerkinElmer LLC
710 Bridgeport Avenue
Shelton, Connecticut 06484-4794
U.S.A.
Or emailed to: [email protected]
Notices
The information contained in this document is subject to change without notice.
Except as specifically set forth in its terms and conditions of sale, PerkinElmer makes no
warranty of any kind with regard to this document, including, but not limited to, the implied
warranties of merchantability and fitness for a particular purpose.
PerkinElmer shall not be liable for errors contained herein for incidental consequential damages in connection
with furnishing, performance or use of this material.
Copyright Information
This document contains proprietary information that is protected by copyright.
All rights are reserved. No part of this publication may be reproduced in any form whatsoever or translated
into any language without the prior, written permission of PerkinElmer, Inc.
Copyright © 2006 PerkinElmer, Inc.
Produced in the USA.
Trademarks
Registered names, trademarks, etc. used in this document, even when not specifically marked as such, are
protected by law.
PerkinElmer is a registered trademark of PerkinElmer, Inc.
e-ssentials is a trademark of PerkinElmer, Inc.

Table of Contents
Introduction......................................................................................5
Note......................................................................................................7
Product Warranty ...................................................................................8
About This Manual................................................................................10
Conventions Used in this Manual............................................................11
Notes, cautions and warnings..........................................................11
Warning Signs on the Instrument...........................................................14
Safety Practices ..............................................................................15
Overview .............................................................................................18
Common Safety Precautions ..................................................................19
Prior to Use ...................................................................................19
In Use...........................................................................................19
Installation, Maintenance, and Relocation.........................................19
Ignition of Flammable Chemicals .....................................................20
Explosion of Vapor from Flammable Chemicals..................................20
Electric Shock Due to Contact with Inside of Instrument....................21
Electric Shock Due to Improper Grounding .......................................21
Injury Due to Xenon Lamp Explosion ...............................................21
Lamp Replacement.........................................................................21
Lamp Installation ...........................................................................22
Lamp Disposal ...............................................................................22
Inflammation or Injury Due to Toxic or Corrosive Solvent..................23
Touching Hot Part Could Result in Burns ..........................................23
Direct Viewing of Xenon Lamp Could Cause Eye Damage...................23
Heavy Instrument ..........................................................................23
Prevention of Instrument Tipover ....................................................23
Precautions on Use of Solvents..............................................................24
Disposal of Waste Solution ....................................................................25
Accuracy and Precision of Measured Values......................................25
Possible Carryover..........................................................................25
Before Turning on Xenon Lamp Again..............................................25
General Operating Conditions ................................................................27
Environmental Conditions......................................................................28
Electrical Safety....................................................................................29
EMC Compliance...................................................................................30
Europe..........................................................................................30
United States.................................................................................30
WEEE Instructions for PerkinElmer Products............................................31
Warning Labels on the Instrument .........................................................32
System Description .........................................................................35
Overview .............................................................................................37
Control Switches and Indicators.......................................................38
Keyboard on the UI Pad (Option).....................................................39
The Rear Panel ..............................................................................42
Description of Fluorometry ....................................................................44
Principle of Fluorometry..................................................................44
Advantages of Fluorometry .............................................................45
Notes on Fluorescence Analysis.......................................................47
Principle of Operation............................................................................51
Features of Light Source.................................................................52
Specifications .......................................................................................53
Installation......................................................................................55
Unpacking............................................................................................57
Installation Conditions...........................................................................58
Installation Space...........................................................................58

Environmental Conditions for An Installation Location........................59
Checking the Contents....................................................................59
Assembling ..........................................................................................60
Removal of Transport Screws..........................................................60
Tubing Arrangements.....................................................................61
UI Pad...........................................................................................62
Spare Parts Available ............................................................................65
Operation ........................................................................................67
General Information about Entering Parameters......................................71
Setup ..................................................................................................72
Selecting the Communication Channel..............................................72
Setting the Liquid Crystal Display Contrast........................................73
Setting of Standard Xenon Lamp Lifetime.........................................74
Operating Instructions...........................................................................76
To Adjust the Auto Zero..................................................................76
Setting the Wavelength ..................................................................76
Setting the Utility ...........................................................................77
Setting the GLP Function.................................................................84
Spectrum Measurement..................................................................91
Setting of Recorder and Spectrum Output Factors.............................95
Operation of Marker .......................................................................98
Setting of Time Program.................................................................98
Edit the Wavelength Program ..............................................................105
Shutdown Procedure...........................................................................108
Flushing the Flow Cell After Use...........................................................109
Before Performing Analysis..................................................................110
Characteristics of Organic Solvents ................................................112
Cautions on Static Electricity .........................................................116
Maintenance..................................................................................117
Operational Check ..............................................................................119
Performance Check.............................................................................120
Energy Check of Xe Lamp .............................................................120
Check of Wavelength Accuracy by Using Hg Lamp ..........................120
Check of Sensitivity ......................................................................120
Check and Calibration of Wavelength Accuracy...............................130
Check of Leak Sensor ...................................................................132
Periodic Checks ..................................................................................134
Maintenance & Inspection Methods......................................................135
Energy Check of Xe Lamp .............................................................135
Wavelength Accuracy Check Using Hg Lamp...................................135
Check of Lamp Logbook................................................................135
Check and Washing of Flow Cell Unit....................................................136
Check and Washing of Flow Cell....................................................136
Disassembly and Cleaning of Flow Cell...........................................138
Leakage from the Flow Cell...........................................................140
Cautions on Storage of the Flow Cell..............................................141
Lamp Replacement.............................................................................142
How to Replace the Xenon Lamp...................................................142
Fuse Replacement ..............................................................................153
Troubleshooting............................................................................155
Warnings ...........................................................................................157
Troubleshooting .................................................................................158
Error Messages...................................................................................160
Appendix .......................................................................................163
DESCRIPTION OF CONTACT SIGNALS..................................................165
Outline........................................................................................165
TERMINOLOGY...................................................................................166
Index.............................................................................................171

Introduction

Note
Be sure to read through and understand the following points with regard to this User’s Guide.
1. Information contained in this document is subject to change without notice for improvement.
2. No part of this manual may be reproduced, transmitted or disclosed to a third party in any form
or by any means without the express written permission of PerkinElmer, Inc.
3. PerkinElmer, Inc. assumes no liability for any direct, indirect, or consequential damages arising
from use not described in this manual.
4. Utmost care must be exercised when using the instrument.
5. This document does not provide any warranty or permission for industrial properties or any
rights to grant license lawfully and without infringement.
This instrument is intended for use by persons having a basic knowledge of chemical analysis
procedures. Keep in mind that improper use of analytical instruments, chemicals or samples would
result not only in wrong analytical data but also in consequences adverse to safety.
Please read this instruction manual carefully before attempting operation and acquaint yourself with
the Series 200a FL detector for its correct use.
The liquid chromatograph system comprises a pump unit for delivering eluents, a sample
introduction unit for injecting samples (autosampler, manual injector), a column unit for separating
components of each sample, a column oven unit for maintaining the column at a constant
temperature, and a detector unit for detecting separated sample components as electric signals.
Before using the liquid chromatograph system, read carefully the instruction manual accompanying
each of these units so that you can carry out analysis properly.

8 . Series 200a User’s Guide
Product Warranty
This product is warranted to be free from defects in material or workmanship under normal use
within the product specifications indicated in this manual and under conditions given below.
This warranty is void if the instrument is not used according to the instruction manual.
The manufacturer makes no warranties, either express or implied, except as provided herein,
including without limitation thereof, warranties as to marketability, merchantability, for a particular
purpose or use, or against infringement of any patent.
No oral or written information or advice given by the manufacturer, its dealers, distributors, agents
or employees shall create a warranty or in any way increase the scope of this warranty.
1. Scope of Warranty:
Any parts that prove to be defective in design or workmanship during the warranty period
will be repaired, adjusted or replaced without charge. A substitute part may be used for
repair, or replacement with an equivalent product may be made instead of repair. Such
system components as a personal computer and printer to be updated frequently for
improvement may not be available in original versions at the time of replacement.
Note that this warranty does not apply to the instrument after it is discarded, or if modified
by the user or resold without permission from the manufacturer, consumable parts, and
any failure of lifetime-expired parts. The manufacturer assumes no liability for any damage
to data or application software due to any possible fault or failure of this instrument.
2. Warranty Period:
One year from the date of initial installation (In case a separate warranty document has
been issued, the warranty period indicated in it takes precedence over the above period)
3. Limitations and Exclusions on Warranty. Note that the following cases are excluded from
the scope of this warranty.
a. Failure due to operation at a place not meeting the installation requirements
specified by the manufacturer.
b. Failure due to power supply voltage/frequency other than specified by the
manufacturer or due to abnormality in power supply.
c. Corrosion or deterioration of the piping due to impurities contained in gas,
compressed air or cooling water supplied by the user.
d. Corrosion of the electric circuits or deterioration of the optical elements due to
highly corrosive atmospheric gas.
e. Failure due to use of software, hardware or spare parts not supplied by the
manufacturer.
f. Failure due to use not described in the manual or improper repair not approved by
the manufacturer.

Introduction 9
g. Failure due to maintenance or repair by other than service personnel qualified by
the manufacturer.
h. Failure due to relocation or transport conducted not under the supervision of the
manufacturer after the initial installation of the instrument.
i. Failure due to disassembly, modification or relocation not approved by the
manufacturer.
j. Failure due to acts of God, including fire, earthquake, storm, flood, lightning, social
disturbance, riot, crime, insurrection, terrorism, war (declared or undeclared),
radioactive pollution, contamination with harmful substances, etc.
k. Failure of the hardware, or damage to the system software, application software or
data due to computer virus infection.
l. After disposal of this instrument, after its resale without prior approval from the
manufacturer, consumable parts, and failure of any part that has reached the end
of its service life.
4. Disclaimer of Warranty
THE MANUFACTURER MAKES NO WARRANTIES, EITHER EXPRESS OR IMPLIED, EXCEPT
AS PROVIDED HEREIN, INCLUDING WITHOUT LIMITATION THEREOF, WARRANTIES AS
TO MARKETABILITY, MERCHANTABILITY, FOR A PARTICULAR PURPOSE OR USE, OR
AGAINST INFRINGEMENT OF ANY PATENT. IN NO EVENT SHALL THE MANUFACTURER BE
LIABLE FOR ANY DIRECT, INCIDENTAL OR CONSEQUENTIAL DAMAGES OF ANY NATURE,
OR LOSSES OR EXPENSES RESULTING FROM ANY DEFECTIVE PRODUCT OR THE USE OF
ANY PRODUCT.
NO ORAL OR WRITTEN INFORMATION OR ADVICE GIVEN BY THE MANUFACTURER, ITS
DEALERS, DISTRIBUTORS, AGENTS OR EMPLOYEES SHALL CREATE A WARRANTY OR IN
ANY WAY INCREASE THE SCOPE OF THIS WARRANTY.

About This Manual
This user’s guide is divided into following chapters:
Chapter 1 Introduction
This chapter contains the product warranty, conventions used in the manual and the conventions
and warnings used in the manual.
Chapter 2 Safety Practices
Important safety information is provided in this chapter.
Chapter 3 System Description
This chapter contains information on the detector, a descriptions of Fluorometry and instrument
specifications.
Chapter 4 Installation
Information on installing and re-installing your instrument should you ever need to move your
system is provided.
Chapter 5 Operation
This chapter provides information on operating the instrument.
Chapter 6 Maintenance
Maintenance and cleaning procedures for the various components of your instrument are provided.
Chapter 7 Troubleshooting
Troubleshooting information, software and system error messages are provided for the instrument.
Appendix
The miscibility chart of solvents is in this appendix.

Introduction . 11
Conventions Used in this Manual
Normal text is used to provide information and instructions.
Bold text refers to text that is displayed on the screen.
UPPERCASE text, for example, ENTER or ALT, refers to keys on the PC keyboard. '+' is used to
show that you have to press two keys at the same time, for example, ALT+F.
All eight digit numbers are PerkinElmer part numbers unless stated otherwise.
Notes, cautions and warnings
Three terms, in the following standard formats, are also used to highlight special circumstances and
warnings.
NOTE:
A note indicates additional, significant information that is provided with some procedures.

12 . Series 200a User’s Guide
CAUTION
We use the term CAUTION to inform you about situations that could
result in serious damage to the instrument or other equipment.
Details about these circumstances are in a box like this one.
D
Caution (Achtung)
Bedeutet, daß die genannte Anleitung genau befolgt werden muß, um
einen Geräteschaden zu vermeiden.
DK
Caution (Bemærk)
Dette betyder, at den nævnte vejledning skal overholdes nøje for at
undgå en beskadigelse af apparatet.
E
Caution (Advertencia)
Utilizamos el término CAUTION (ADVERTENCIA) para advertir sobre
situaciones que pueden provocar averías graves en este equipo o en
otros. En recuadros éste se proporciona información sobre este tipo de
circunstancias.
F
Caution (Attention)
Nous utilisons le terme CAUTION (ATTENTION) pour signaler les
situations susceptibles de provoquer de graves détériorations de
l'instrument ou d'autre matériel. Les détails sur ces circonstances
figurent dans un encadré semblable à celui-ci.
I
Caution (Attenzione)
Con il termine CAUTION (ATTENZIONE) vengono segnalate situazioni
che potrebbero arrecare gravi danni allo strumento o ad altra
apparecchiatura. Troverete informazioni su tali circostanze in un riquadro
come questo.
NL
Caution (Opgelet)
Betekent dat de genoemde handleiding nauwkeurig moet worden
opgevolgd, om beschadiging van het instrument te voorkomen.
P
Caution (Atenção)
Significa que a instrução referida tem de ser respeitada para evitar a
danificação do aparelho.

Introduction . 13
WARNING
We use the term WARNING to inform you about situations that could
result in personal injury to yourself or other persons. Details about
these circumstances are in a box like this one.
D
Warning (Warnung)
Bedeutet, daß es bei Nichtbeachten der genannten Anweisung zu
einer Verletzung des Benutzers kommen kann.
DK
Warning (Advarsel)
Betyder, at brugeren kan blive kvæstet, hvis anvisningen ikke
overholdes.
E
Warning (Peligro)
Utilizamos el término WARNING (PELIGRO) para informarle sobre
situaciones que pueden provocar daños personales a usted o a
otras personas. En los recuadros como éste se proporciona
información sobre este tipo de circunstancias.
F
Warning (Danger)
Nous utilisons la formule WARNING (DANGER) pour avertir des
situations pouvant occasionner des dommages corporels à
l'utilisateur ou à d'autres personnes. Les détails sur ces circonstances
sont données dans un encadré semblable à celui-ci.
I
Warning (Pericolo)
Con il termine WARNING (PERICOLO) vengono segnalate situazioni
che potrebbero provocare incidenti alle persone. Troverete
informazioni su tali circostanze in un riquadro come questo.
NL
Warning (Waarschuwing)
Betekent dat, wanneer de genoemde aanwijzing niet in acht wordt
genomen, dit kan leiden tot verwondingen van de gebruiker.
P
Warning (Aviso)
Significa que a não observância da instrução referida poderá causar
um ferimento
ao usuário.

14 . Series 200a User’s Guide
Warning Signs on the Instrument
WARNING
FOR CONTINUED PROTECTION
REPLACE ONLY WITH SAME FUSE
TYPE AND RATING
Power On/Off push button
Alternating current

Safety Practices

Overview
This chapter describes the general safety practices and precautions that must be observed when
operating the Series 200a Fluorescence Detector.
This advice is intended to supplement, not supersede, the normal safety codes in the user's
country. It is also a supplement to the PerkinElmer standard Safety and Health Policy. The
information provided does not cover every safety procedure that should be practiced. Ultimately,
maintenance of a safe laboratory environment is the responsibility of the analyst and the analyst's
organization.
Please consult all manuals supplied with the Series 200a and accessories before you start working
with the instrument. Carefully read the safety information in this chapter and in the other manuals
supplied. When setting up the instrument or performing analyses or maintenance procedures,
strictly follow the instructions provided.

Safety Practices . 19
Common Safety Precautions
Prior to Use
•Before using the instrument, be sure to read this user’s guide carefully to attain a full
understanding of its operations.
•Keep this user’s guide handy nearby so it can be referred to whenever needed.
•Be sure to observe the procedures specified in this user’s guide.
•Be sure to understand and follow all the safety instructions given in this user’s guide.
•Be sure to observe all the hazard warnings attached to the instrument or provided in this
user’s guide. Failure to do so could result in personal injury or damage to the instrument.
•Be sure to follow all the methods of use instructed in this user’s guide for proper
application of the product.
•Absolutely avoid modifying the product, using non-specified parts, or removing safety
devices as it could be hazardous.
•Do not perform any operation or action other than described in this user’s guide. If you
have any trouble in the instrument, notify the nearest sales representative or service office
of PerkinElmer.
•When using chemicals for the instrument, be sure to provide proper ventilation of the
room. Inadequate ventilation could endanger human health.
•Keep in mind that the hazard warnings in the manuals or on the product cannot cover
every possible case, as it is impossible to predict and evaluate all circumstances
beforehand. Always be alert and use your common sense.
In Use
If an abnormality such as unusual noise, odor, fuming or gas leakage occurs during
operation of the instrument, immediately disconnect power to the instrument, and take
proper safety measures as required. Then, contact your PerkinElmer service representative.
Installation, Maintenance, and Relocation
•At the time of delivery, installation of the instrument shall be carried out by or under
supervision of qualified service personnel of the manufacturer or its authorized service
agent for ensuring safety and high accuracy in operation of the instrument. It is not
permitted for the user to carry out installation.
•After completion of installation, check that all the standard parts are provided. If the
instrument is made active with any one of the standard parts not equipped, a failure could
occur which may result in a hazardous condition.
NOTE:
If any item is missing or damaged or if you have any questions, please notify PerkinElmer.

20. Series 200a Fi User’s Guide
•The maintenance and checkup procedures to be taken by the user are only those described
in the manual. When taking the maintenance and checkup procedures described in the
manual, attain a clear understanding of them.
•Do not perform other maintenance and checkup procedures to avoid jeopardizing safety
and causing troubles in the instrument.
•After installation, do not relocate the instrument. If the instrument is relocated, vibration or
impact applied during relocation could cause a malfunction in the optical components that
have been adjusted precisely.
•If any warning/caution label has become illegible due to deterioration with age or it has
been damaged due to any cause, contact your PerkinElmer service representative.
WARNING
We use the term WARNING to inform you about situations that
could result in personal injury to yourself or other persons. Be
sure that all instrument operators read and understand the
precautions listed below. It is advisable to post a copy of the
precautions near or on the instrument shelf.
Ignition of Flammable Chemicals
•This instrument is not explosion-proof. In unattended operation, do not use organic
solvents having an ignition point below 70 °C.
•Beware of ignition hazard when using flammable chemicals such as organic solvents.
a. Do not bring a heat or flame source near the instrument.
b. Well-ventilate the laboratory room where the instrument is used.
c. Always check the following conditions. If an abnormality is found, stop operation
immediately and unplug the power cord from the supply.
¾Leakage of solvent or waste solution.
¾Leakage of solvent inside the instrument.
•When using flammable chemicals, be careful about possible ignition due to static electricity.
To prevent the build-up of static electricity, use a conductive container for waste solution
and provide proper grounding connection to it.
Explosion of Vapor from Flammable Chemicals
If a flammable chemical such as organic solvent leaks from the flow path of the instrument and its
vapor concentration reaches the explosion limit, it could cause spontaneous combustion with
dangerously explosive results.
When using a flammable and readily volatile chemical, be sure to check for leakage from the
instrument flow path and ventilate the laboratory room adequately.