PerkinElmer Spotlight 150 User manual

Spotlight 150
User’s Guide
MOLECULAR SPECTROSCOPY

Release History
Part Number Release Publication Date
L1050099 D October 2015
Any comments about the documentation for this product should be addressed to:
User Assistance
PerkinElmer, Inc.
710 Bridgeport Avenue
Shelton, Connecticut 06484-4794
U.S.A.
Or emailed to: [e-mail]
Notices
The information contained in this document is subject to change without notice.
Except as specifically set forth in its terms and conditions of sale, PerkinElmer makes no
warranty of any kind with regard to this document, including, but not limited to, the
implied warranties of merchantability and fitness for a particular purpose.
PerkinElmer shall not be liable for errors contained herein for incidental consequential damages in
connection with furnishing, performance or use of this material.
Copyright Information
This document contains proprietary information that is protected by copyright.
All rights are reserved. No part of this publication may be reproduced in any form whatsoever or
translated into any language without the prior, written permission of PerkinElmer, Inc.
Copyright © 2015 PerkinElmer, Inc.
Produced in the U.S.A.
Trademarks
Registered names, trademarks, etc. used in this document, even when not specifically marked as such,
are protected by law.
PerkinElmer is a registered trademark of PerkinElmer, Inc.
Fluorolube is a registered trademark of the Hooker Chemical Corporation
Nujol is a registered trademark of the Esso Standard Oil Company
Spotlight, Frontier, and Spectrum are trademarks of PerkinElmer, Inc.

Contents
Introduction............................................................................................... 5
About this Manual ............................................................................................. 6
Conventions Used in this Manual ........................................................................ 7
Notes, Cautions and Warnings..................................................................... 7
Definitions.................................................................................................10
Related Documents....................................................................................10
Requirements for using the Spotlight 150....................................................10
Connecting the PC to the Local Area Network..............................................10
Warnings and Safety Information ...........................................................11
The Spotlight 150.............................................................................................12
Summary.........................................................................................................13
General Operating Conditions............................................................................14
Electrical Safety ...............................................................................................15
Location and Ventilation ...................................................................................16
Warning Labels ................................................................................................17
Microscope Safety Labels ...........................................................................17
Warning Signs on the Microscope ...............................................................18
Lifting the Spotlight 150 ...................................................................................19
EMC Compliance ..............................................................................................20
System Requirements.......................................................................................21
Electrical Requirements..............................................................................21
Safety Specifications.........................................................................................22
Microscope................................................................................................22
Overview of the Spotlight 150 ................................................................. 23
A Guided Tour of the Spotlight 150 ...................................................................24
Connections .....................................................................................................25
Operation ........................................................................................................26
The Optical System ..........................................................................................27
Visible Light Optics ....................................................................................27
Infrared Optics ..........................................................................................30
Getting Ready to Use the Spotlight 150 .................................................. 33
Before Using the Spotlight 150..........................................................................34
Cooling the MCT Detector .................................................................................35
Setting up the Spotlight 150 .............................................................................38
Focusing the Microscope ............................................................................39
Fitting an Attenuator to the Spectrometer ...................................................41
Setting Scan Parameters ............................................................................42
Preparing Samples ................................................................................... 45
Preparing Samples ...........................................................................................46
Tools for Sample Preparation ............................................................................47
Tools Provided with the Spotlight 150 .........................................................47
Other Useful Tools.....................................................................................48
Specialized Accessories ..............................................................................49
Items to Have Available .............................................................................49
Common Window Materials...............................................................................50
Techniques for Preparing Samples for Transmission Measurements.....................51
Flattening Solids ........................................................................................51
Slicing Samples from Solids ........................................................................52
Polymers...................................................................................................53
Particles ....................................................................................................55
Fibers .......................................................................................................56
Fibrous Solids ............................................................................................56
Coatings on Substrates ..............................................................................57
Liquids ......................................................................................................57
Techniques for Collecting Spectra ........................................................... 59
Techniques for Collecting Spectra......................................................................60

Using the Compression Cell ....................................................................... 60
Collecting a Spectrum from a Thick Sample ................................................ 61
Collecting a Spectrum in an Inert Atmosphere ............................................ 62
Viewing a Sample with the Visible Polarizer Accessory........................................ 64
The Theory of Light Polarization................................................................. 64
Applications .............................................................................................. 65
Equipment................................................................................................ 66
Collecting IR Spectra Using the Infrared Polarizer Accessory .............................. 68
Using the Polarizer .................................................................................... 69
Collecting Spectra With the ATR Objective ........................................................ 70
ATR Objective Specification .............................................................................. 72
Fitting the ATR Crystal Holder to the Microscope...................................................... 73
Measuring the Energy in Reflectance Mode ....................................................... 75
Adjusting the Height of the Crystal ................................................................... 76
Centering the Crystal................................................................................. 77
Checking the Infrared Alignment................................................................ 78
Removing the ATR Crystal Holder ..................................................................... 79
Fitting a Crystal Assembly to the ATR Objective................................................. 80
Manual ATR Objective ............................................................................... 80
Automated ATR Objective.......................................................................... 80
Cleaning the ATR Objective Crystal ................................................................... 82
Auto ATR Cleaning Procedure........................................................................... 83
Materials Needed ...................................................................................... 83
Cleaning Procedure ................................................................................... 83
Collecting Spectra with the ATR Crystal............................................................. 88
Manual ATR Objective ............................................................................... 88
Automated ATR Objective.......................................................................... 89
Reflectance FT-IR Microspectroscopy ................................................................ 91
Diffuse Reflectance ................................................................................... 91
Specular Reflectance ................................................................................. 92
Reflection-Absorption ................................................................................ 93
Maintenance.............................................................................................94
Maintenance.................................................................................................... 95
Inspecting the Microscope ......................................................................... 95
Protecting the Microscope.......................................................................... 96
Cleaning ................................................................................................... 96
Cleaning the Cover.................................................................................... 96
Replacing the Microscope Fuse ......................................................................... 97
Service............................................................................................................ 98
Accessories ..................................................................................................... 99
Sample Preparation Tools .......................................................................... 99
Electrical Connections .................................................................................... 100
Fitting the Plug ....................................................................................... 100
Connecting the Microscope to the Electrical Supply ................................... 101
Connecting the Microscope to the Spectrometer........................................ 101
Appendices .............................................................................................102
Appendix 1: Decontamination and Cleaning..................................................... 103
Appendix 2: WEEE Instructions for PerkinElmer Products ................................. 104
Index............................................................................................................ 105

Introduction

6 . Spotlight 150 User's Guide
About this Manual
This introduction gives you information about this manual, to enable you to use it effectively
when learning to operate the Spotlight 150.
Before you start using your Spotlight 150 we recommend that you:
•Read the
Warnings and Safety Information starting on page 11;
•Read the rest of this
Introduction and become aware of conventions and requirements;
•Read
Overview of the Spotlight 150 starting on page 23;
•Read Getting Ready to Use the Spotlight 150 starting on page 33.
Four chapters in this User’s Guide contain information on using and maintaining your
Spotlight 150:
•
Getting Ready to Use the Spotlight 150 gives you information on how to set up your
Spotlight 150 at the beginning of the day's work, to make sure that it is working
properly.
•
Preparing Samples describes techniques for preparing many types of microscopic
samples. It includes descriptions of the sample preparation tools provided with, or
available for, the Spotlight 150.
•
Techniques for Collecting Spectra describes how you can use accessories and different
techniques to collect spectra.
•
Maintenance contains maintenance information. It gives you information on how to care
for the Spotlight 150 and about the performance checks that we recommend that you
perform routinely.

Introduction . 7
Conventions Used in this Manual
Normal text is used to provide information and instructions. Italic text is used to provide
commentary. Bold text refers to text that is displayed on the screen.
UPPERCASE text, for example ENTER or ALT, refers to keys on the PC keyboard. “+” is
used to show that you have to press two keys at the same time, for example, ALT+F.
All eight digit numbers are PerkinElmer part numbers unless stated otherwise.
“Spectrometer” refers to the Frontier IR Systems, Spectrum 400 Series, Spectrum 100 Series,
Spectrum 65 or Spectrum One spectrometer supplied with your Spotlight 150.
Notes, Cautions and Warnings
Three terms, in the following standard formats, are also used to highlight special
circumstances and warnings.
NOTE: A note indicates additional, significant information that is provided with some
procedures.

8 . Spotlight 150 User's Guide
CAUTION
We use the term CAUTION to inform you about situations that could result
in serious damage to the instrument
or other equipment. Details about
these circumstances are in a box like this one.
Caution (Achtung)
Bedeutet, daß die genannte Anleitung genau befolgt werden muß, um einen
Geräteschaden zu vermeiden.
Caution (Bemærk)
Dette betyder, at den nævnte vejledning skal overholdes nøje for at undgå
en beskadigelse af apparatet.
Caution (Advertencia)
Utilizamos el término CAUTION (ADVERTENCIA) para advertir sobre
situaciones que pueden provocar averías graves en este equipo o en otros.
En los recuadros como éste se proporciona información sobre este tipo de
circunstancias.
Caution (Attention)
Nous utilisons le terme CAUTION (ATTENTION) pour signaler les
situations susceptibles de provoquer de graves détériorations de
l'instrument ou d'autre matériel. Les détails sur ces circonstances figurent
dans un encadré semblable à celui-ci.
Caution (Attenzione)
Con il termine CAUTION (ATTENZIONE) vengono segnalate situazioni
che potrebbero arrecare gravi danni allo strumento o ad altra
apparecchiatura. Troverete informazioni su tali circostanze in un riquadro
come questo.
Caution (Opgelet)
Betekent dat de genoemde handleiding nauwkeurig moet worden opgevolgd,
om beschadiging van het instrument te voorkomen.
Caution (Atenção)
Significa que a instrução referida tem de ser respeitada para evitar a
danificação do aparelho.

Introduction . 9
WARNING
We use the term WARNING to inform you about situations that could
result in personal injury to yourself or other persons. Details about these
circumstances are in a box like this one.
Warning (Warnung)
Bedeutet, daß es bei Nichtbeachten der genannten Anweisung zu einer
Verletzung des Benutzers kommen kann.
Warning (Advarsel)
Betyder, at brugeren kan blive kvæstet, hvis anvisningen ikke overholdes.
Warning (Peligro)
Utilizamos el término WARNING (PELIGRO) para informarle sobre
situaciones que pueden provocar daños personales a usted o a otras
personas. En los recuadros como éste se proporciona información sobre
este tipo de circunstancias.
Warning (Danger)
Nous utilisons la formule WARNING (DANGER) pour avertir des
situations pouvant occasionner des dommages corporels à l'utilisateur ou
à d'autres personnes. Les détails sur ces circonstances sont données dans
un encadré semblable à celui-ci.
Warning (Pericolo)
Con il termine WARNING (PERICOLO) vengono segnalate situazioni
che potrebbero provocare incidenti alle persone. Troverete informazioni
su tali circostanze in un riquadro come questo.
Warning (Waarschuwing)
Betekent dat, wanneer de genoemde aanwijzing niet in acht wordt
genomen, dit kan leiden tot verwondingen van de gebruiker.
Warning (Aviso)
Significa que a não observância da instrução referida poderá causar um
ferimento ao usuário.

10 . Spotlight 150 User's Guide
Definitions
OPERATOR: Person operating the equipment for its intended purpose.
RESPONSIBLE BODY: Individual or group responsible for the use and maintenance of the
equipment and for ensuring that the OPERATORS are adequately trained.
Related Documents
Manuals for Spectrometer
Information on using your spectrometer can be found in the manuals that were supplied with
it. The
IR & Raman Manuals CD (L1050002) includes instrument manuals for the Frontier IR
Systems, Spectrum 400 Series and Spectrum 100 Series spectrometers. The
Spectrum Two
Manuals CD
(L1050242) contains the instrument manual for the Spectrum Two spectrometer.
Spectrum Software
Spectrum software has an on-screen Help system, which you can access by selecting
Contents from the Help menu, by using the F1 key, or by clicking the Help button in a
dialog. The Help information assumes that you are familiar with the hardware components of
the Spotlight 150 microscope contained in this guide.
Requirements for using the Spotlight 150
We assume that the Spotlight 150 has been properly set up and aligned. This installation
must be performed by a PerkinElmer Service Representative.
Ensure that your PC meets the minimum requirements for hardware and software set out in
the
PC Requirements section of the Spectrum Administrator’s Guide (L1050095).
Connecting the PC to the Local Area Network
The PC provided is connected point-to-point to the Spotlight 150. If you wish to connect the
PC to your local area network to enable transfer of data, we recommend that you contact
your local PerkinElmer Service Representative.

Warnings and Safety
Information

12 . Spotlight 150 User's Guide
The Spotlight 150
The Spotlight 150 consists of a microscope, FT-IR spectrometer and a PC.
Figure 1 Spotlight 150 – Microscope and Frontier IR System
Figure 2 Spotlight 150 – Microscope and Spectrum Two System

Warnings and Safety Information . 13
Summary
The PerkinElmer Spotlight 150 has been designed to comply with a wide variety of
international standards governing the safety of laboratory equipment. In routine use, the
Spotlight 150 poses virtually no risk to you. If you take some simple, common-sense
precautions, you can make sure that you maintain the continued safe operation of the
Spotlight 150.
•DO make sure that all parts of the Spotlight 150 are properly connected to the electrical
supply; in particular, make sure that the ground (earth) wires are securely connected.
•DO disconnect the electrical power supply before opening the main cover of the
microscope and spectrometer.
•DO keep the microscope dry. Avoid spilling liquid into the microscope and spectrometer.
Clean all external spills immediately. If anything that is spilled enters the main body of
the microscope or spectrometer, switch off the power and call a PerkinElmer Service
Representative.
•If your Spotlight 150 is fitted with an MCT (mercury cadmium telluride) detector,
DO wear safety glasses and protective gloves when you are filling the detector dewar in
the microscope with liquid nitrogen. Slowly pour the liquid nitrogen into the dewar.
Stand back from the detector during filling because liquid nitrogen may be expelled
from the dewar flask. Use only liquid nitrogen.
•DO NOT use a flammable gas to purge the spectrometer or microscope. The spectrometer
contains a hot lamp, and fire or explosion may result. Use only clean, dry, oil-free
nitrogen or air to purge the instrument.
•DO read the more detailed information on safety in the following pages.
WARNING
Removal of covers and fixtures not specified in this manual may result in
hazardous radiation exposure.
Use of controls or adjustments or performance of procedures other than
those specified herein may result in hazardous radiation exposure.

14 . Spotlight 150 User's Guide
General Operating Conditions
For information about the spectrometer, refer to the User’s Guide supplied with the
instrument. The
IR & Raman Manuals CD (L1050002) includes instrument manuals for the
Frontier IR Systems, Spectrum 400 Series and Spectrum 100 Series spectrometers.
The microscope has been designed and tested in accordance with PerkinElmer specifications
and in accordance with the safety requirements of the International Electrotechnical
Commission (IEC). The microscope conforms to IEC publication 61010−1 (“Safety
requirements for electrical equipment for measurement, control, and laboratory use”) as it
applies to IEC Class 1 (earthed) appliances and therefore meets the requirements of EC low
voltage directive 2006/95/EC.
Only use the microscope indoors and under the following conditions:
Temperature 15 °C to 35 °C
Relative humidity 80% maximum (non-condensing)
If possible, avoid any adjustment, maintenance and repair of the opened, operating
instrument. If any adjustment, maintenance and repair of the opened instrument is
necessary, this must be done by a skilled person who is aware of the hazard involved.
Whenever it is likely that the microscope is unsafe, make it inoperative. The microscope may
be unsafe if it:
•Shows visible damage;
•Fails to perform the intended measurement;
•Has been subjected to prolonged storage in unfavorable conditions;
•Has been subjected to severe transport stresses.
The microscope has been designed to be safe under the following conditions:
•Indoor use;
•Altitude up to 2000 m;
•Ambient temperatures of 5 °C to 40 °C;
•A maximum ambient relative humidity of 80% for temperatures up to 31 °C decreasing
linearly to 50% relative humidity at 40 °C;
•Mains fluctuations not exceeding ±10% of the nominal voltage. For example, 230 V ± 10%.
WARNING
If the equipment is used in a manner not specified herein the protection
provided by the equipment may be impaired.

Warnings and Safety Information . 15
Electrical Safety
Connect the microscope to a power supply line that includes a switch or other adequate
means of disconnection from the electricity supply.
Plug the microscope into electricity-supply sockets that are provided with a protective ground
(earth) connection only. The microscope must be earthed.
If fuses need replacing, use only those with the required current rating and of the specified
type. Do not use makeshift fuses and do not short-circuit fuse holders.
When the microscope is connected to its electricity supply, terminals may be live. Removing
covers other than those which can be removed by hand is likely to expose live parts.
NOTE: There are no user-serviceable parts in the microscope.
Capacitors inside the microscope may still be charged even if the microscope has been
disconnected from all voltage sources.
Disconnect the microscope from all voltage sources before opening for any adjustment,
replacement, maintenance or repair. Any adjustment, replacement, maintenance or repair
must be performed by a PerkinElmer Service Representative.
The microscope must only be connected to equipment meeting the requirements of
IEC 61010-1 (Safety requirements for electrical equipment for measurement, control and
laboratory use – general requirements) or IEC 60950 (Safety of information technology
equipment).
WARNING
Any interruption of the protective ground (earth) conductor inside or
outside the microscope
or disconnection of the protective ground (earth)
terminal can make the microscope dangerous.

16 . Spotlight 150 User's Guide
Location and Ventilation
The Spotlight 150 is installed by a PerkinElmer Service Representative, who will be able to
advise on the positioning of the system. To allow for adequate cooling, the system should
not be sited near to room heating equipment, for example, central-heating radiators. There
should be a minimum gap of at least 15 cm (6 inches) from the top and side surfaces of the
microscope to permit adequate cooling.
WARNING
Make sure that the switch at the electrical supply inlet at the rear of the
microscope is not obstructed.

Warnings and Safety Information . 17
Warning Labels
When this label is attached to an instrument it means “Caution, risk of
danger”. Refer to the manual to find out the nature of the potential
hazard and any actions which have to be taken.
Microscope Safety Labels
The following safety labels are fixed to the microscope.
Figure 3 Rear of Microscope
Figure 4 Inside Dewar lid – MCT detector versions only
NOTE: See Cooling the MCT Detector on page 35 for information on how to fill the Dewar.
Power
100–230 V ~50/60 Hz
75 VA

18 . Spotlight 150 User's Guide
Warning Signs on the Microscope
Caution, risk of electric shock.
Caution, risk of danger.
Refer to accompanying documents to find out the nature of the potential
hazard and any actions which have to be taken.

Warnings and Safety Information . 19
Lifting the Spotlight 150
WARNING
The microscope and spectrometer are heavy precision instruments, so
two people are required for safe handling.
Consult the local codes of practice issued by safety advisors before
attempting to lift these instruments.
Take care not to injure yourself or others, or to drop the instruments.
The microscope weighs approximately 32 kg.
•Lift the microscope only by the base.
•Do not attempt to lift it by the stage, cassegrain assembly or other attachments.
The spectrometer weighs approximately 34 kg unpacked and has a lifting recess on either
side.
Do not move the Spotlight 150 after it has been installed without consulting your local
PerkinElmer Service Representative.

20 . Spotlight 150 User's Guide
EMC Compliance
EC directive
The Spotlight 150 has been designed and tested to meet the requirements of the EMC
Directive 2004/108/EC.
The Spotlight 150 complies with the EMC standard EN61326, (EMC standard for electrical
equipment for measurement, control and laboratory use) and EN55011 (ISM) class A
(rf emissions).
FCC rules and regulations
This product is classified as a digital device used exclusively as industrial, commercial, or
medical test equipment. It is exempt from the technical standards specified in Part 15 of the
FCC Rules and Regulations, based on Section 15.103(c).