PerkinElmer Lambda 365 User manual

09931462A
PerkinElmer, 710 Bridgeport Avenue,
Shelton, CT06484-4794, U.S.A
Produced in the USA.
Lambda 365 5-Pos Variable Pathlength Cell Holder
Installation Instructions
This instruction sheet describes the installation of this accessory which is used with the Lambda 365
Spectrophotometer.
NOTE:
Read these instructions before you install this accessory.
Contacting PerkinElmer
Supplies, replacement parts, and accessories can be ordered directly from PerkinElmer, using the part
numbers.
See our website:
http://perkinelmer.com
PerkinElmer's catalog service offers a full selection of high-quality supplies.
To place an order for supplies and many replacement parts, request a free catalog, or ask for information:
If you are located within the U.S., call toll free 1-800-762-4002, 8 a.m. to 8 p.m. EST. Your order will be
shipped promptly, usually within 24 hours.
If you are located outside of the U.S., call your local PerkinElmer sales or service office.
Features
• Available with various pathlength cells (5, 10, 20, 40, 50 mm)
• Excellent durability
Figure 1 Lambda 365 5-Pos Variable Pathlength Cell Holder [P/N: 4100104]

09931462A
2
Dimensions and Specifications
Dimensions
Physical Characteristic Specification Comment
Outline
Height (mm) 139.5
Width (mm) 134.5
Depth (mm) 341
Inner
Height (mm) 35
Suitable for the Standard
Cells
Width (mm) 58
Depth (mm) 12.6
Weight (kg) 2.5
Specification
Physical Characteristic Specification Comment
Space between cells (mm) 7.4
Moving distance of one cell (mm) 20
Moving distance of five cells (mm) 80
Moving time of one cell (sec) 4
Moving time of five cells (sec) 11
Description
Connectable Cells
Description
Precision cell, 5 mm light path, 1.7 ml, ea
Precision cell, 10 mm light path, 3.5 ml, ea
Precision cell, 20 mm light path, 7 ml, ea
Precision cell, 40 mm light path, 14 ml, ea
Precision cell, 50 mm light path, 17.5 ml, ea
Other cells are available upon request if what you want is not listed.

09931462A
3
Configuration of the 5-Pos Variable Pathlength Cell Holder
Figure 2 Lambda 365 5-Pos Variable Pathlength Cell Holder
Variable Partition
Variable Pathlength Cuvette Holder
①5 mm cell
②10 mm cell
③20 mm cell
④40 mm cell
⑤50 mm cell
Figure 3 Lambda 365 5-Pos Variable Pathlength Cell Holder

09931462A
4
- Used to fix a cell holder, a base plate or a front plate for Lambda 365.
- Spare screws (2ea) are enveloped with the accessory.
Figure 4 Phillips round head screw with washer (M4 *12L)
Installation
1. Prepare the Lambda 365 Spectrophotometer to install this accessory.
2. Connect the power cord and the communication cable. DO NOT turn on the power of the
instrument!
Figure 5 Connecting the power cord and USB communication cable
3. Remove the two Phillips round head screws with washer (M4*12L) to remove the existing cell
holder and base plate.
Figure 6 Location of the two Phillips round head screws with washer
CAUTION
ATTENTION
Make sure the instrument is turned off while installing this accessory.
Assurez-vous que l’instrument est éteint lors de l’installation de
cet
accessoire.

09931462A
5
4. Pull out the cell holder and base plate by hand.
Figure 7 Pulling out the cell holder and base plate
5. Insert the 5-Pos Variable Pathlength Cell Holder in the sample compartment.
Figure 8 Inserting the 5-Pos Variable Pathlength Cell Holder in the sample
compartment

09931462A
6
6. Gently press the 5-Pos Variable Pathlength Cell Holder to connect the communication port (male)
under the bottom of the 5-Pos Variable Pathlength Cell Holder to the port (female) in the sample
compartment.
Figure 9 Connecting the communication ports
7. Tighten the 5-Pos Variable Pathlength Cell Holder in the sample compartment with the screws
(The red circles indicates the location of the screws in the figure).
Figure 10 Tighten the 5-PosVariable Pathlength Cell Holder in the sample
compartment
8. Turn on the power switch of the instrument.

09931462A
7
Measurement
NOTE: Before executing System Self Test, the 5-Pos Variable Pathlength Cell Holder has to be installed first.
Otherwise, the instrument can be damaged electrically and do not operate properly.
1. Install the 5-Pos Variable Pathlength Cell Holder referring to chapter Installation.
2. Close the sample compartment cover and turn on the instrument.
CAUTION
ATTENTION
Do not start the UV Express software before finishing the initialization of grating.
Ne démarrez pas
le logiciel UV Express avant de terminer l'initialisation du
réseau.
NOTE: Start the System Self Test after warming up the system for at least 20 minutes.
3. Double-click on the UV Express software folder and select one of the modes for starting.
4. The following message box will be displayed. Empty the cell holder and close the lid firmly. Click
OK.
5. System Self Test starts. Click OK after finishing the System Self Test.
6. Click New to open a new window. Select Experiment Type and click OK.
7. Open Method in the main menu.

09931462A
8
8. ①Click the Accessory tab and ②Select Variable Pathlength Multi-Cell on Accessory
Type, and then click the ③Method Setup button to open the Multicell window.
9. The Multicell window will be shown. Using the button, select cells to be used among the
available cells list. Check (√) Multi Baseline, if it is required to use.
a. Available Cells: Indicate cells that are available for measurement.
b. Using Cells: Shows the cell positions which are selected for measurement. Remove cells
using key and switch positions using and keys.
c. Multicell Options
Multi Zero: If checked (√), zero will be measured at all the selected cell positions. If not,
zero will be only measured at the 1st cell position among the using cells.
Multi Baseline: If checked (√), the baseline will be measured at each selected cell. If not
checked, the baseline is only measured at the 1st cell position among the using cells.
NOTE:
To prevent inadequate baseline (zero) measurement because of the difference in transmittance among
cuvettes or any other reasons, Multi Baseline (Multi Zero) measurement is recommended.
10. Click OK.
11. After setting parameters for Experiment, Lamp, Internal Reference and Scan Setup, click
Apply and then select OK.
12. After placing the reference solution into both the selected Using Cell positions and the reference
cell position of the Multicell holder, select the Baseline or Zero buttons to perform the Baseline
function.
NOTE:
The baseline is required to run only one time when the first measurement is done, not every
measurement. However, it should be done whenever the system is power off/on, the wavelength is
changed, SBW is changed or Reference sample is changed.

09931462A
9
13. Place the reference into the reference cell position and sample into the selected using cell
positions.
14. Select the Sample button to measure.
15. After the measurement is finished, the results are displayed in the result window. Save or print
results as required.
Calibration of Multi-Cell Position
Calibrate the beam position of the 5-Pos Variable Pathlength Cell Holder when the multicell is installed for
the first time or beam position is incorrect.
1. Execute Scan Setup mode and ①click the Accessory tab of the Method window. ②Select
Variable Pathlength Multi-Cell on Accessory Type, and then click the ③Method Setup
button to open the Multicell window.
2. Select the Calibration tab in the Multicell window. The following dialog box will appear.

09931462A
10
3. The functions of the Multicell calibration are shown as follows.
Command Function
MultiCell Position Show saved steps about each cell position of the Multi-Cell.
Multicell Move
Used for moving Multi-Cell position as clicking buttons
.
Used for moving Multi-Cell position using
buttons by
entered step.
Multicell Calibration Used to perform the Multi-Cell calibration.
Stop Used to stop the Multi-Cell calibration.
Save Result Used to save the calibrated result.
Reset Multicell Used to move to ‘0’ step of Multi-Cell position.
4. Click on Reset Multicell to format the Multi-Cell steps. Click OK.
5. Select MultiCell Calibration , and then the following dialog box will appear. Remove all
samples from the 5-Pos Variable Pathlength Cell Holder (Empty the multicell holder).
6. Select OK, and then the Multicell calibration will start. The current process of calibration will be
shown in the main window.

09931462A
11
7. When calibration is finished, the following box appears. Select OK.
8. Select Save Result to save the result. If the following message box appears, select OK.
Troubleshooting
When the 5-Pos Variable Pathlength Cell Holder does not move
1. Check the Connector status.
Check if the 5-Pos Variable Pathlength Cell Holder connector is connected firmly to the Lambda
365.
When the intensity value is low
1. Recalibrate the 5-Pos Variable Pathlength Cell Holder.
Recalibrate if the light beam does not go through the center of the cell holder hole.
2. Replace the lamp.
If the intensity value is still low after the recalibration, the lamps of the light source for
measurement may be deteriorated and need replacing. Contact PerkinElmer.

09931462A
12
When the Multi-Cell does not reset
If the resetting is failed, the photo interrupter switch inside the 5-Pos Variable Pathlength Cell Holder
needs replacing. Contact PerkinElmer for replacing the photo interrupter.