Radleys Greenhouse plus User manual

nstructions

Green ouse Plus™

Parallel Synthesiser

Cat No. RR99610

Your Local Distributor

July 2018 ssue 4

Radleys Discovery Technologies

Shire Hill, Saffron Walden, Essex, CB11 3AZ. United Kingdom.

Tel: +44-(0)1799-513320 Fax: +44-(0)1799-513283

Email: sales@radleys.co.uk Web: www.radleys.com

innovations for chemistry

Page 1

RR99610 - GreenHouse Plus Parallel Synthesiser

Index

Page 1 Index

Page 2 Introduction

Page 3 Safety Information

Important WARNINGS

Page 4 Products & Accessories

Page 5 Green ouse Plus Exploded View

Page 6 Green ouse Plus Applications

V-Mag Stirring Technology

Page 7 Safety Pressure Relief Valve

IMPORTANT Safety procedures for Safety Pressure Relief Valve

Page 8 Green ouse Plus Assembly Instructions

Page 9 Green ouse Plus Assembly Instructions - continued

Selecting the Septum and Sealing Mat for your application

Page 10 Green ouse Plus Assembly Instructions - continued

Page 11 Green ouse Plus Operating Instructions

Page 12 Green ouse Plus Operating Instructions - continued

Page 13 Green ouse Plus Operating Instructions - continued

Green ouse Plus Chemical Resistance

Page 14 Gasket Selection & Maintenance

Page 15 Gasket Selection & Maintenance - continued

Page 16 Important WARNINGS

Page 17 WARRANTY - EMAIL BACK

Please refer to Green ouse Plus Cooling Reservoir Instructions for details of Cooled Reactions

RR99610 - GreenHouse Plus Parallel Synthesiser

Thank you for purchasing your Green ouse Plus Parallel Synthesiser

Please read this Instruction Manual thoroughly before operating your unit.

Introduction

The Green ouse Plus brings all the benefits in productivity of multiple parallel synthesis to the chemist

at a fraction of the cost of automated systems.

The patented GreenHouse Plus allows chemists to perform 24 reactions in parallel, with a reaction volume of 0.5 to 7ml

per reaction tube. The GreenHouse Plus’s removable reaction block holds 24 glass reaction tubes and has the same

footprint as a standard micro titer plate. This allows the rapid transfer of samples to and from micro titer plates

with a multi-channel pipettor or robotic system. The reaction block is also designed to fit directly into leading

vacuum centrifuges including Genevac.

The GreenHouse Plus is designed to mount on a standard KA or Carousel stirring hotplate, utilising the

single rotating magnetic field to vigorously stir individual PTFE magnetic stirring bars in each

of the 24 glass reaction tubes.

The stirring hotplate provides even heating to all 24 glass reaction tubes (ambient to +160°C solution temperature),

with an accuracy of +/-0.5°C achievable when using the optional fuzzy logic digital temperature controller.

The combined reflux and additions head allows for convenient additions or withdrawals from individual reaction

tubes whilst under reflux and whilst maintaining an inert atmosphere.

Highly effective refluxing within all 24 individual glass reaction tubes is provided by the aluminium head

with it’s water cooling and nickel condensing fingers.

The head features quick release connectors for easy connection to your water supply, and an easy to use septum

mat for additions and withdrawals.

The head features a nickel plated safety pressure relief valve that vents at 4 to 6psi.

Cooled reactions to -70°C are achieved using the optional GreenHouse Cooling Reservoir filled with acetone

and dry ice (or other solvent and dry ice combinations). The removable cooling reservoir fits between the

stirrer and GreenHouse assembly, allowing simultaneous stirring and cooling.

A neat inert gas system with one inlet/outlet for vacuum and inert gas, allows reactions to be performed under

inert atmospheres with ease. Also because there is no flow of gas through the chamber there is minimal

evaporation of solvents during synthesis.

Warranty

GreenHouse Plus Parallel Synthesiser includes one year full parts and labour warranty from date of original

purchase. Warranty will only be valid if a completed Warranty Email Back is returned within 1 month

of date of purchase (see last page).

n the event of product failure please contact your local distributor.

Please do not return any goods without prior agreement.

Page 2

Safety Information

The following symbols are intended to assist the user in the safe and efficient

operation of the GreenHouse Plus Parallel Synthesiser.

Important WARNINGS

Please read these instructions completely before using your GreenHouse unit.

Operate only in a fumehood with protective safety sash.

The GreenHouse should only be operated by trained and competent personnel. As with all chemistries, a full risk

assessment should be performed prior to starting, and care should be taken to monitor your reactions at all stages.

During operation the GreenHouse should not be left unattended unless in a supervised area.

Maximum recommended operating temperature is 150˚C.

Risk of burns. Take care not to touch the GreenHouse during or after heating. The GreenHouse unit will remain hot

for some considerable time after the heating source has been switched off. A temperature probe or temperature sensitive

label can be used to indicate when components are too hot to touch.

Pressure

This product is not suitable for pressurised reactions. DO NOT PRESSUR SE ABOVE 3psi.

Safety Relief Valve vents at 4 to 6psi. Do not tamper with or obstruct Safety Relief Valve.

Do not use glass chamber if it is scratched, chipped or cracked.

Chemical Resistance

The GreenHouse Plus’ nickel plating may be subject to chemical attack under certain conditions.

Please see Page 13 for full details before using your Green ouse.

The GreenHouse Plus units are coated with a high specification nickel plating. This plating has been developed over many

years with the plating process refined to improve chemical resistance to the optimimum level. Whilst the plating specification

offers excellent resistance to most chemical attack, unfortunately it is not possble to guarantee that it is 100% resistant to all

chemical attack. There will always be some cases where particularly aggressive chemicals or chemical cocktails will attack

the nickel. Therefore we are unable to provide a definitive list of chemicals that may cause damage to the GreenHouse

because of the variability of conditions, such as temperature, exposure time, reflux conditions etc.

Please ensure that there is always adequate cooling of the reflux head during synthesis, this will help minimise the

creation of vapours and minimise chemical attack. Even with ambient or unheated synthesis, vapours can

cause chemical attack of the nickel.

Never overheat the system above the recommended temperature of 150˚C as this will encourage chemical attack.

RR99610 - GreenHouse Plus Parallel Synthesiser

Page 3

Warning

Applies when there is a possibility

of personal injury.

Important Note

Alerts the user to important facts.

mportant, mportant,

Page 4

RR99610 - GreenHouse Plus Parallel Synthesiser

Products & Accessories

Cat No Description Pk Qty

Green ouse Plus Parallel Synthesiser

RR99610 GreenHouse Plus Parallel Synthesiser 1

Components & Accessories

RR99611 GreenHouse Plus Reaction Block 1

RR99612 GreenHouse Plus Reaction Tubes 100

RR99613 GreenHouse Plus PTFE Magnetic Stirring Bar 40

RR99614 GreenHouse Plus Silicone Septum Mat 3

RR99615 GreenHouse Plus PTFE Faced Silicone Septum Mat 3

RR99616 GreenHouse Plus Pre-Slit PTFE Faced Silicone septum Mat 3

RR99617 GreenHouse Plus PTFE Faced Butyl Rubber Sealing Mat 3

RR99618 GreenHouse Plus Septa + Sealing Mat Evaluation Kit 4

RR99602 GreenHouse Cooling Reservoir 1

RR99605 Replacement Viton Gaskets 2

RR99606 Replacement Silicone Gaskets 2

RR99664 Replacement Glass Cylinder 1

RR99905 Digital Thermometer (-250˚C to +400˚C) & 200mm Probe 1

Carousel Stirring otplate & Temperature Controller

RR98072 Carousel Stirring Hotplate 230v & UK plug 1

RR98073 Carousel Temperature Controller 1

Green ouse Plus Systems:

Include all the important components and accessories required for efficient operation...

RR99619 System 27 - Green ouse Plus Parallel Synthesiser

Comprises of the following

RR99610 GreenHouse Plus Parallel Synthesiser 1

RR98072 Carousel Stirring Hotplate 230 volt & UK Plug 1

RR98073 Carousel Temperature Controller 1

RR99612 GreenHouse Plus Reaction Tubes 100

RR99613 GreenHouse Plus PTFE Magnetic Stirring Bars 40

RR99618 GreenHouse Plus Septa + Sealing Mat Evaluation Kit 4

RR99620 System 28 - Green ouse Plus Parallel Synthesiser with Cooling Reservoir

Comprises of the following

RR99610 GreenHouse Plus Parallel Synthesiser 1

RR98072 Carousel Stirring Hotplate 230 volt & UK Plug 1

RR98073 Carousel Temperature Controller 1

RR99602 GreenHouse Cooling Reservoir 1

RR99612 GreenHouse Plus Reaction Tubes 100

RR99613 GreenHouse Plus PTFE Magnetic Stirring Bars 40

RR99618 GreenHouse Plus Septa + Sealing Mat Evaluation Kit 4

RR99061 Reaction Flask Support Ring 6

RR99905 Digital Thermometer (-250 to +400˚C) 200mm Probe 1

Green ouse Plus Exploded View

RR99628 - Sealing Knobs

RR99606 - Silicone Gasket

(RR99605 - Optional Viton Gasket)

Greenhouse Plus PTFE

Faced Butyl Rubber Sealing

Mat - RR99617

Sealing Plate

Sealing Plate Knobs

GreenHouse Plus Septa

+ Sealing Mat Evaluation

Kit - RR99618

RR99606 - Silicone Gasket

(RR99605 - Optional Viton Gasket)

RR99612 - Reaction Tubes

RR99664 - Glass Cylinder

Base Unit

RR98072 - Carousel

Stirring Hotplate

Lower Guide Pillars

Combined Reflux and

Additions Head

Solid nickel Reflux Pins

Reaction Block - RR99611

O-Ring for Sealing

Knobs - RR99635

RR99610 - GreenHouse Plus Parallel Synthesiser

Page 5

The Green ouse Plus Parallel Synthesiser can be set-up in

two basic configurations:

eated and Ambient Reactions

For reactions at ambient temperatures and above use the water cooled

GreenHouse Plus head with nickel condensing fingers for highly effective

refluxing within all 24 individual glass reaction tubes. The single gas

inlet/outlet port on the Reflux/Additions Head allows the easy removal of air by

vacuum and the introduction of inert gas. To add air sensitive reagents use the

Septum mat at ambient temperature prior to heating to reflux.

Cooled Reactions (see separate nstructions)

For reactions at temperatures below ambient use the GreenHouse Plus with

the GreenHouse Cooling Reservoir (optional extra). Use of an inert gas is

normally essential for such reactions, as typically very reactive air or moisture

sensitive reagents are used. The Septum mat allows the addition of reagents

during synthesis and whilst maintaining fully inert conditions. The single gas

inlet/outlet port on the head allows the easy removal of air by vacuum and the

introduction of inert gas.

RR99610 - GreenHouse Plus Parallel Synthesiser

Warning

The Green ouse Plus’ nickel plating may be subject to

chemical attack under certain conditions. Please see Page 13

for full details before using your Green ouse.

Warning

The Green ouse Plus Parallel Synthesiser is not designed for

pressurised reactions.

Pressurised reactions may cause explosion.

DO NOT PRESSURISE ABOVE 3psi

Please refer to Green ouse

Cooling Reservoir

Instructions for details

of Cooled Reactions

V-Mag Stirring Technology

The GreenHouse Plus uses V-Mag stirring technology, which utilises oversized

vertically positioned PTFE stirring bars to maximise the uniformity of stirring

within each reaction tube. The stirring bars actually spin in a v-shaped orbit.

Page 6

Green ouse Plus Applications

Important Note

Care should be taken not to over load the tubes with too much

solid material, such as scavenger resins, as this may restrict

the effectiveness of the stirring.

mportant, mportant,

The Safety Pressure Relief Valve is plated internally and externally with

electroless nickel plating giving it superior chemical resistance to most solvents

and acids, when compared with unplated aluminium or stainless steel.

The Safety Pressure Relief Valve contains a Hastelloy (C276) spring and Kalrez

(flouropolymer) sealing O-ring.

All Safety Pressure Relief Valves are individually tested and certified to crack at

4 to 6psi +/-5%.

1. On every occasion, prior to use of your GreenHouse Plus ensure that the

Safety Pressure Relief Valve is not stuck to its seating. This is achieved by

gently pulling up the cap until the valve comes off its seating.

2. Use only a regulated inert gas supply not exceeding 3psi. For efficient inert

reactions you should require only a very slight positive pressure of inert gas,

say 0.5 to 1psi. Excessive gas pressures are dangerous and unnecessary.

3. Do not attempt to dismantle or modify the Safety Pressure Relief Valve. This

is not a user serviceable item and must only be replaced with an approved

replacement.

4. The Safety Pressure Relief Valve is designed to vent at nominally 4 to 6psi.

Therefore under normal and correct operating procedures (where maximum

recommended operating pressure is 3psi) the Safety Pressure Relief Valve

should not be venting.

5. Should over-pressure venting occur:

a. mmediately turn off gas supply

b. Either disconnect gas inlet or lift Relief Valve to remove excess pressure.

c. mmediately turn off any heating, and if possible remove GreenHouse

Plus from hotplate (use heat resistant gloves).

d. Check that incoming gas does not exceed 3psi.

e Check that reflux cooling is sufficient.

f. Check temperature setting of hotplate.

6. Always ensure that a reliable cooling water supply is used during refluxing.

Failure of cooling water during heating/refluxing may lead to over-heating.

Overheating of the system can encourage attack of the nickel plating by

aggressive liquids or vapours.

RR99610 - GreenHouse Plus Parallel Synthesiser

Page 7

Warning

The Green ouse Plus Parallel Synthesiser is not designed for

pressurised reactions.

Pressurised reactions may cause explosion.

DO NOT PRESSURISE ABOVE 3psi

IMPORTANT

Safety Procedures for Safety Pressure Relief Valve

Safety Pressure Relief Valve

Locate GreenHouse Plus base on to stirring hotplate (maximum top plate

diameter 135mm). The circular recess in the base fits snugly around the top

plate, yet allows it to be lifted on or off the stirring hotplate with ease.

Use the two sealing knobs to seal the head and ensure a gas tight chamber. The

sealing knobs need only to be hand tight.

Using the locating pins slide the head down into position on the glass

chamber, taking care to align alpha-numeric numbering on head and base.

Ensure that the circular gasket is properly located in its groove, then carefully

place glass chamber on to silicone (or optional Viton) gasket.

Fill reaction block with reaction tubes. Add PTFE magnetic stirring bar to each

reaction tube. Add solvents and reagents to the tubes as required, taking note of

the appropriate rack position (A1-D6) for each sample.

nsert reaction block into GreenHouse Plus base, taking care to align alpha-

numeric numbering on base and reaction block.

RR99610 - GreenHouse Plus Parallel Synthesiser

Page 8

Warning

Do not use glass chamber if it is scratched, chipped or

cracked, as even the smallest flaw could lead to failure.

Important Note

Take care to align the locating pins first to ensure that the

head does not damage your reaction tubes as you slide it in

position.

mportant, mportant,

Important Note

The Green ouse Plus has a thin electroless nickel plating for

protection against chemical attack. Damage to this plating via

scratches or chips, or the use of some strong acids, may lead

to corrosion of the underlying aluminium metal.

mportant, mportant,

Important Note

Over tightening may damage the thread. Do not use a spanner

or wrench to tighten sealing knobs.

mportant, mportant,

Green ouse Plus Assembly Instructions

RR99610 - GreenHouse Plus Parallel Synthesiser

Selecting the Septum and Sealing mat for your application

There are three septum mats and one sealing mat available for use with the

GreenHouse Plus. All four mats have been manufactured specifically to fit the

GreenHouse Plus, and have one pre-punched centre hole allowing for easy

insertion of a temperature probe through the mat and into the reaction block.

RR99614 - Greenhouse Plus Silicone Septum Mat

A basic silicone elastomer septum mat. The silicone exhibits excellent resistant

to heat and cold as well as offering good resistance to a broad range of solvents

and solvent vapours. Can be repeatedly pierced whilst maintaining a gas

tight seal.

RR99615 - PTFE Faced Silicone Septum Mat

Manufactured from chromatographically clean silicone elastomer, with a thin PTFE film on both sides offering excellent

chemical resistance.

RR99616 - Pre-Slit PTFE Faced Silicone Septum Mat

Manufactured from chromatographically clean silicone elastomer, with a thin PTFE film on both sides and 24 pre-slit positions

enabling use of blunt syringe needles.

RR99617 - PTFE Faced Butyl Rubber Sealing Mat

Manufactured from a tough PTFE coated butyl rubber. Designed to use for sealing the GreenHouse Plus when

additions/removals are not required. Providing an inert, gas tight environment for samples. The PTFE face is bonded to one

side of the durable butyl rubber providing an inert chemically resistant barrier. This sealing mat should be used with the PTFE

coated side facing downwards towards the reaction vessels.

RR99618 - Septa + Sealing Mat Evaluation Kit

An evaluation kit containing one each of the above four septum/sealing mats.

Page 9

Select the appropriate septum/sealing mat and place in recess. Now place

sealing plate in the correct position and seal with four knobs. (See below for

assistance on septum/sealing mat selection).

Important Note

When placing septum mat into recess on Green ouse Plus

head, always ensure that pre-punched centre hole in mat

aligns with the probe hole in the head.

mportant, mportant,

Green ouse Plus Assembly Instructions - continued

6b 6c 6d

RR99610 - GreenHouse Plus Parallel Synthesiser

For accurate temperature control we recommend using the RR98073 Digital

Temperature Controller. Connect the RR98073 Fuzzy Logic Digital Temperature

Controller connection (D N 12 878 Class 2) to the connection port at the rear of

the stirring hotplate.

Connect your water hose onto the barbed end of two swivel elbow connectors

provided. Then simply insert the nozzle into the quick release water connector

on the head. Either water connector may be used for in/out water flow.

For most applications cold tap water should be adequate for efficient cooling.

However when low boiling point solvents are used or in regions with elevated

ambient temperatures a chilled water supply is recommended.

Maximum recommend cooling water supply pressure is 10psi.

Carefully insert the temperature probe down into the reaction block; through the

pre-punched centre hole sealing septum, taking care to insert the end of the

probe in the corresponding hole in the reaction block below.

10b

10a

connection contact ther-

mometer

mains power

connection

mains

connection

Warning

Read RR98072 Carousel Stirring otplate and RR98073 Fuzzy

Logic Digital Temperature Controller instructions thoroughly

before operation.

Page 10

Green ouse Plus Assembly Instructions - continued

Important Note

If the probe will not easily insert, then stop and check that you

have correctly aligned the septum mat with the hole below.

mportant, mportant,

Connect your gas/vacuum tube to the hose barb connector provided. Then

simply insert the other end into the quick release gas fitting on the head.

Warning

Use only a regulated inert gas supply not exceeding 3psi. For

efficient inert reactions you should require only a very slight

positive pressure of inert gas, say 0.5 to 1psi. Excessive gas

pressures are dangerous and unnecessary.

Warning

Aggressive solvents or vapours (such as trifluoroacetic acid (TFA)) will corrode the (glass filled, reinforced

Nylon) gas fitting.

Warning

Always ensure that an adequate and reliable cooling water

supply is used during synthesis, this will help minimise the

creation of vapours and minimise chemical attack. Even with

ambient or unheated synthesis, vapours can cause chemical

attack of the nickel.

Step 1

Assemble apparatus as detailed on pages 8, 9 and 10.

Step 2

Prior to use of your GreenHouse Plus ensure that the Safety Pressure Relief

Valve is not stuck to its seating. This is achieved by gently pulling up the cap until

the valve comes off its seating.

Step 3

Turn on cooling water supply. For most applications tap water should be

adequate for efficient cooling. However when low boiling point solvents are used

or in regions with elevated ambient temperatures a chilled water supply is

recommended.

RR99610 - GreenHouse Plus Parallel Synthesiser

Page 11

Step 4

Using a 3-way tap connect the GreenHouse Plus to a regulated inert gas

supply (e.g. nitrogen or argon) and a vacuum source (e.g. water pump or house

vacuum etc).

The GreenHouse Plus is first evacuated to the best vacuum that can be attained

then it is filled with the inert gas using the 3-way tap. Repeating this twice more

will purge the chamber and provide an inert atmosphere.

Warning

Always ensure that an adequate and reliable cooling water

supply is used during synthesis, this will help minimise the

creation of vapours and minimise chemical attack. Even with

ambient or unheated synthese, vapours can cause chemical

attack of the nickel.

Warning

The Green ouse Plus should only be operated in a fume

cupboard with protective safety sash.

Important Note

Maximum recommended cooling water supply pressure is

10psi.

mportant, mportant,

Green ouse Plus Operating Instructions

Warning

Use only a regulated inert gas supply not exceeding 3psi. For

efficient inert reactions you should require only a very slight

positive pressure of inert gas, say 0.5 to 1psi. Excessive gas

pressures are dangerous and unnecessary.

Warning

Agressive solvents or solvents vapours such as trifluoroacetic

acid (TFA) will corrode the (glass filled reinforced Nylon) gas

fitting.

Step 5

Set the stirring speed and temperature to the desired

level. To obtain the best temperature control, use the

optional RR98073 Fuzzy Logic Digital Temperature

Controller to maintain the set temperature to +/- 0.5˚C.

Maximum recommended operating temperature is 150˚C.

RR99610 - GreenHouse Plus Parallel Synthesiser

Warning

Please read RR98072 Carousel Stirring

otplate and RR98073 Fuzzy Logic

Digital Temperature Controller instruc-

tions thoroughly before operation.

Warning

The Safety Pressure Relief Valve is designed to vent at nomi-

nally 4 to 6psi. Therefore under normal and correct operation

procedures (where maximum recommended operating pressure

is 3psi) the Safety Pressure Relief Valve should not be venting.

Should over-pressure venting occur:

a. mmediately turn off gas supply

b. Either disconnect gas inlet or lift Relief Valve to remove excess pressure.

c. mmediately turn off any heating, and if possible remove GreenHouse Plus from hotplate (use heat resistant gloves).

d. Check that incoming gas does not exceed 3psi.

e Check that reflux cooling is sufficient.

f. Check temperature setting of hotplate.

Page 12

Adding Air Sensitive Reagents During Synthesis

Step 6

Before making your reagent additions, it is important to relieve any pressure

within the chamber, otherwise you may not be able to inject the reagent or there

may be a splash-back through the septum mat when you withdraw the needle.

To do this carefully lift Relief Valve to remove excess pressure

Step 7

To prolong the life of your septum mat, always wipe your injecting needle before

insertion to remove any excess reagent that may attack the mat. Also avoid

inserting tip of the needle into your sample where it can similarly become

contaminated with solvent residue.

Step 8

Care should be taken to monitor your synthesis, gas supply, cooling water and

reaction temperature.

Important Note

Always hold the syringe vertically to ensure that you are

injecting into the reaction tube directly below.

mportant, mportant,

Green ouse Plus Operating Instructions - continued

RR99610 - GreenHouse Plus Parallel Synthesiser

Page 13

Warning

Take care when disassembling your Green ouse Plus as surfaces may be hot. Also take care to minimise

solvent spillage and scratching or chipping of nickel plated components.

Step 9

Once your synthesis is complete:

a. Turn off gas supply.

b. Disconnect gas inlet or lift Relief Valve to remove excess pressure.

c. Turn off heating.

d. f possible remove GreenHouse from hotplate (use heat resistant gloves), otherwise heat transfer will continue.

e. Once reaction has sufficiently cooled, turn off reflux cooling.

f. The sealing knobs can now be unscrewed and the reaction block removed.

The GreenHouse Plus units are coated with a high specification nickel plating. This plating has been developed over many

years with the plating process refined to improve chemical resistance to the optimimum level. Whilst the plating specification

offers excellent resistance to most chemical attack, unfortunately it is not possble to guarantee that it is 100% resistant to all

chemical attack. There will always be some cases where particularly aggressive chemicals or chemical cocktails will attack

the nickel. Therefore we are unable to provide a definitive list of chemicals that may cause damage to the GreenHouse

because of the variability of conditions, such as temperature, exposure time, reflux conditions etc.

To Reduce the Risk of Corrosion we recommend the following:

1. Exposure times should be kept to aminimum i.e. do not leave the unit exposed to the chemicals or vapours for any longer

than absolutely necessary.

2. Relux cooling should always be used, even if not heating. Always ensure that an adequate and reliable cooling water

supply is used during synthesis, this will help minimise the creation of vapours and minimise chemical attack. Even with

ambient or unheated synthesis, vapours can cause chemical attack of the nickel.

3. Use inert gas. The slight positive pressure of the inert gas decreases the levels of evaporation, plus the purging of the

system will remove water vapour in the chamber (the combination of water and acids such as HCl can accelerate corrosion).

4. The units should always be thoroughly cleaned after use.

5. Care should be taken to ensure that the nickel plating is not damaged or scratched.

6. Care should be taken to avoid spillages of acids directly onto the nickel plating.

7. f any evidence of corrosion occurs you should stop using the unit, review your methods and chemistry to identify the cause,

and where necessary contact Radleys

Green ouse Plus Operating Instructions - continued

Warning

Strong acids like Cl, Br and acetic acid, particularly in the form of hot vapour, may cause corrosion.

Increased temperatures, exposure times and the presence of water will accelerate this corrosion.

Warning

If any evidence of corrosion occurs you should stop using the unit, review your methods and chemistry

to identify the cause, and where necessary contact Radleys

Important Note

If you have any doubt over the chemical resistance of the Green ouse in relation to your application,

please contact Radleys and we will provide you with a sample of the nickel plated aluminium for testing.

mportant, mportant,

Please refer to Green ouse Plus Cooling Reservoir Instructions for details of Cooled Reactions

Green ouse Plus Chemical Resistance

Gasket Introduction

The GreenHouse Plus is supplied as standard with, Silicone Rubber Gaskets

(RR99605) in the Base Unit and the Reflux/Additions Head. These gaskets are

suitable for the majority of applications but in some cases the optional Viton

Rubber Gaskets (RR99606) will be more suitable.

When to use the Silicone Rubber Gaskets

The Silicone Rubber Gaskets will be suitable for most applications showing

good resistance to most common organic solvents and reagents including

acetone, acetonitrile, alcohols, acetic acid, esters, DMF, DMSO, mineral acids

and bases etc. t should be borne in mind that resistance testing is normally done

with the material totally immersed in the solvent, whereas with the GreenHouse

Plus in normal operation the gaskets should only be exposed to low

concentrations of vapours from solvents and reagents.

An exception to this is when the GreenHouse Cooling Reservoir is used with dry

ice/acetone as the cooling medium, where the gasket in the GreenHouse Plus

Base Unit will come in direct contact with acetone. The Silicone Rubber Gasket

is particularly suitable for this application as it stays soft at low temperatures and

shows good resistance to acetone.

Silicone Rubber shows poor resistance to trifluoroacetic acid (TFA) and therefore

Viton may be preferred for applications involving this solvent.

When to use the Viton Rubber Gaskets

Viton shows superior resistance to chlorinated hydrocarbons such as

dichloromethane and chlorobenzene compared with Silicone Rubber. t is also

more resistant to aromatic hydrocarbons. Viton exhibits relatively good resis-

tance to trifluoroacetic acid (TFA) and is the preferred gasket in this case.

Viton shows poor resistance to acetone and also hardens at low temperatures.

Viton gaskets should therefore not be used with the GreenHouse Cooling

Reservoir when dry ice/acetone is used as the cooling agent.

Thermal Resistance of Gaskets

Both the Silicone Rubber and Viton Gaskets have service temperatures well in

excess of the normal maximum recommended operating temperature of the

GreenHouse (ca 150˚C).

RR99610 - GreenHouse Plus Parallel Synthesiser

Page 14

Please refer to Green ouse

Plus Cooling Reservoir

Instructions for details of

Cooled Reactions

Gasket Selection & Maintenance

ow long should the gasket last?

The gaskets are a consumable item and they will need replacing. How long they

last depends very much on what you expose them to. Repeatedly splashing

solvents and reagents directly on to the gaskets will greatly reduce their life.

Conversely careful control of the refluxing will reduce vapours during synthesis

and therefore reduce chemical attack of the gaskets during synthesis.

RR99605 - Silicone Rubber Gaskets, pack of 2

RR99606 - Viton Rubber Gaskets, pack of 2

Changing the Gaskets

The Gaskets should be inspected regularly and replaced when they show signs

of deterioration or embrittlement. They can easily be removed with a pin or

something similar, taking care not to damage the groove in which they seat. The

new gaskets are then simply pushed into place ensuring that they are properly

located in the machined grooves.

You will not require glue or sealant to hold the gaskets in place.

RR99610 - GreenHouse Plus Parallel Synthesiser

Page 15

Gasket Selection & Maintenance - continued

RR99610 - GreenHouse Plus Parallel Synthesiser

Page 16