Sakura Cyto-Tek 2500 User manual

Cyto-Tek
®
2500
Cytocentrifuge
Operating
Manual

Operating
Manual
Cytocentrifuge
Cyto-Tek®
2500

The brand names of products that have been registered or trademarked by
and are owned by Sakura Finetek USA, Inc., Sakura Finetek Japan Co., Ltd.,
and Sakura Finetek Europe B.V. appear here:
Cyto-Tek
Sakura
Tissue-Tek
© 2015 Sakura Finetek USA, Inc.
All Rights Reserved
Printed in U.S.A.
Revised 21 Dec 2015
0007129-01 Rev. C
Manufactured for:
Sakura Finetek USA, Inc., Torrance, CA 90501 U.S.A.
Sakura Finetek Japan Co., Ltd., Tokyo, 103-0023, Japan
Sakura Finetek Europe B.V., 2408 AV Alphen aan den Rijn, NL

TABLE OF CONTENTS
Page - 1 -
1. INTRODUCTION
1.1 Intended Use................................................................................................................................................................................... 3
1.2 General Description........................................................................................................................................................................ 3
1.3 Physical Characteristics.................................................................................................................................................................. 3
1.3.1 Components and Figures .......................................................................................................................................................3
1.3.2 Guard Bowl Unit ...................................................................................................................................................................... 4
1.3.3 Control Panel........................................................................................................................................................................... 5
1.4 Specifications.................................................................................................................................................................................. 6
1.5 Safety Standards ............................................................................................................................................................................ 6
2. INSTALLATION AND SET UP
2.1 General Information........................................................................................................................................................................ 9
2.2 Installation Requirements .............................................................................................................................................................. 9
2.3 Unpacking........................................................................................................................................................................................ 9
2.4 Unpacking the Instrument ..............................................................................................................................................................9
2.5 Standard Accessories and Options..............................................................................................................................................12
2.5.1 Standard Accessories ........................................................................................................................................................... 12
2.5.2 Options/Consumables........................................................................................................................................................... 12
2.5.3 Missing or Damaged Items?.................................................................................................................................................12
3. OPERATING INSTRUCTIONS
3.1 Operating Instructions .................................................................................................................................................................. 13
3.1.1 Workflow (Figure 3A)............................................................................................................................................................13
3.2 Customization of Settings ............................................................................................................................................................13
3.2.1 Creating a Program ...............................................................................................................................................................13
3.3 Assembling the Specimen Chamber and Dispensing the Specimen..........................................................................................16
3.4 How to Load the Rotor.................................................................................................................................................................. 18
3.5 Starting the Operation.................................................................................................................................................................. 19
3.6 Discontinuing the Operation.........................................................................................................................................................20
3.7 Emergency Stop ............................................................................................................................................................................ 20
3.8 Removing the Guard Bowl............................................................................................................................................................20

Page - 2 -
4. CARE OF THE INSTRUMENT
4.1 General Information...................................................................................................................................................................... 23
4.1.1 Cleaning the Control Panel ................................................................................................................................................... 23
4.1.2 Cleaning the Instrument........................................................................................................................................................23
4.1.3 Disassembling and Cleaning the Guard Bowl Unit..............................................................................................................23
4.1.4 Cleaning 6mL/12mL specimen chambers, 6mL/12mL rubber gaskets, 6mL/12mL chamber cap and chamber holder ....26
4.1.5 Gasket Replacement .............................................................................................................................................................27
4.1.6 Preventive Maintenance by a Service Contractor................................................................................................................27
5. TROUBLESHOOTING AND SERVICE
5.1 Power Outage................................................................................................................................................................................ 29
5.2 Actions to be Taken When an Error Occurs.................................................................................................................................29
5.3 Troubleshooting Charts ................................................................................................................................................................ 30
5.3.1 Error Code of Monitored Errors.............................................................................................................................................31
5.3.2 Other Errors ........................................................................................................................................................................... 33
5.4 Service Information....................................................................................................................................................................... 34
5.4.1 When there is a Problem with the Instrument.....................................................................................................................34
5.4.2 Cyto-Tek 2500 Cytocentrifuge PreService Checklist...........................................................................................................34
5.4 Accessory Items............................................................................................................................................................................ 35
5.5 Replacement Parts........................................................................................................................................................................ 35
5.6 Where to Order ............................................................................................................................................................................. 35
6. SAFETY PRECAUTIONS
6.1 Safety Precautions........................................................................................................................................................................ 37
6.1.1 Dangers.................................................................................................................................................................................. 38
6.1.2 Warnings ............................................................................................................................................................................... 39
6.1.3 Cautions................................................................................................................................................................................. 39

1. INTRODUCTION
Page 3
1.1 Intended Use
The Cyto-Tek®2500 Cytocentrifuge is designed for the
purpose of transferring cells onto a glass slide from
cytological specimens contained in a specimen chamber
through the process of centrifugation. The instrument, as
part of the cytology process, is intended to facilitate the in
vitro examination of human and animal cells for morphology
changes by a cytopathologist.
1.2 General Description
The Cyto-Tek 2500 Cytocentrifuge (Figure 1A) is a self-
contained instrument designed to transfer cells from
suspension onto a glass slide by cytocentrifugation. This
instrument has the capacity for up to 12 specimen holders.
Each specimen holder will accommodate a specimen of up
to 12 mL. A mono-layer of cells from the specimen is
deposited on a glass slide in a specific, square area;
therefore, screening of the slide is simplified.
This instrument ensures specimen integrity through
automatic, paced controlled optimal acceleration. The rotor
speed is programmable in 10-rpm increments from 200 rpm
to 2500 rpm. Centrifugation times are programmable from
1 second to 99 minutes 59 seconds. The software can store
up to 30 programs.
The ON-OFF switch, control panel, and digital displays are
all located on the front of the instrument for easy access.
The Cyto-Tek 2500 was designed with enhanced features
focusing on user safety, including a new sealable rotor
housing with lid which significantly reduces aerosol
exposure. The rotor housing is completely removable and
transportable, which enable loading and unloading
specimens away from the cytocentrifuge. The Cyto-Tek
2500 Cytocentrifuge is designed for years of reliable,
efficient, smooth, and quiet operation.
1.3 Physical Characteristics
1.3.1 Components and Figures
(Figure 1B)
The components of the Cyto-Tek 2500 Cytocentrifuge are
shown and described in the following figures and
paragraphs. Read and review them carefully. Locate and
identify each component of this instrument before
attempting to install, program, and operate it.
Figure 1A
③
①
②
⑤
④
⑥
⑦
⑧
⑨
Figure 1B

1. INTRODUCTION
Page 4
①
Power Switch
Turns the instrument power on and off.
②
Control Panel
Provides the control buttons and indicators to display the
instrument status.
③
Instrument Lid
Is opened and closed when the guard bowl unit is placed into the
instrument. A lid interlock mechanism is provided to prevent access
to the guard bowl unit during operation. Also provided are the lid
sensor for detecting that the lid is closed and the lid lock sensor for
detecting that the lid is locked properly. The lid has a lid gasket to
prevent leakage from the guard bowl.
④
Transparent Window
Is provided in the instrument lid to allow the operator to view the
rotation status of the guard bowl.
⑤
Lid Open Push Button
Allows the instrument lid to lift up. By gently pressing the button
located in front of the transparent window, the lid is lifted up and
then opened by operator to the open position. To close the lid,
lower the lid toward the front and push down.
⑥
Emergency Lid Release Opening
⑦
Power Cord Connector (with fuse holders)
Accepts the instrument end of the power cord.
⑧
Lid Gasket
⑨
Spindle
Connects to the bowl shaft housing of the guard bowl to transfer
rotation from the motor.
1.3.2 Guard Bowl Unit (Figure 1C & 1D)
The guard bowl unit is comprised of the guard bowl, rotor,
and bowl lid.
The bowl lid must be put on the guard bowl during handling
and operation.
CAUTION: The guard bowl and bowl lid are adjusted
unit by unit. Avoid using a combination of the guard
bowl with the bowl lid of a different unit.
①
Guard Bowl
Accommodates the rotor. The guard bowl is detachable and has its
own detachable bowl lid.
The bowl lid can reduce aerosols and splashes from specimen
chambers in the rotor during centrifugation.
The rotor is removed from the guard bowl by loosening two
thumbscrews that hold the rotor in place. Splashes from specimen
chambers during centrifugation are accumulated inside the guard
bowl. The guard bowl must be drained after every run.
②
Rotor
Has compartments numbered 1 to 12 as an aid for chamber
management. The rotor fastened to the guard bowl serves as a
stand when fluid samples are dispensed into specimen chambers.
③
Bowl Shaft Housing
④
V-shape Gasket
⑤
Bowl Lid
Securely seals the guard bowl. The lid lock button is provided on
top of the lid knob. The bowl lid is locked by pushing down the
button and can be opened by pulling it up (Figure 1E).
⑥
Bowl Lid Gasket
⑦
Part Number of the Guard Bowl
①
②
④
③
Locked
Unlocked
Lock button
Lid knob
Figure 1C
Figure 1D
Figure 1E

Page 5
1.3.3 Control Panel (Figure 1F)
①
START/STOP key
Starts and stops the operation.
②
SET key
Switches the Standby mode to the Program mode and the Set
& View mode.
③
(down-arrow) key
Changes a program number and decreases a setting value for
the rotation speed or process time.
④
(up-arrow) key
Changes a program number and increases a setting value for
the rotation speed or process time.
⑤
Program Number display (7-segment display)
Displays a program number from 1 to 30.
⑥
Speed display (7-segment display)
Displays a programmed rotation speed or an actual speed.
⑦
Time display (7-segment display)
Displays a programmed process time or a process time left.
⑧
Lid Lock indicator
An orange light is displayed when the instrument lid is locked
prior to the start of operation and goes out when the lid is
unlocked after the motor has stopped.
⑨
Power indicator
Lights up while the power is supplied to the instrument.
①
②
③
④
⑤
⑥
⑦
⑧
⑨
Figure 1F

1. INTRODUCTION
Page 6
1.4 Specifications
Power Required:
Power Rating: AC100 to 240V, 50/60Hz
Power Consumption: 100VA
Power protection: Class I, Pollution Degree 2
Installation Category:
Class II
Weight Main Body:
10 Kg (22 lbs)
Weight: Guard Bowl
1.7Kg (3.7 lbs)
Overall Dimensions:
22.5 cm (8.9 in) high (with lid closed)
59.5 cm (23.4 in) high (with lid opened)
49.0 cm (19.3 in) deep
37.5 cm (14.8 in) wide
Holder Capacity:
12 Specimen Holders
1mL / 6mL / 12mL specimen chambers
Glass Slide Sizes:
Length: 76 +0/-1mm
Width: 26 +0/-1mm
Thickness: 1.1 +0.1/-0.2mm
Rotor Operating Speeds:
From 200 rpm to 2500 rpm; in 10 rpm increments
Programmable Time Settings:
Program memory-up to 30
From 1 second to 99 minutes 59 seconds; selectable in
minutes and seconds
Operating Conditions:
Operational temperature: 10 to 40°C (50 to 104°F)
Operational relative humidity: 30 to 85%RH
(non-condensing)
Storage temperature: 20 to 60°C (–4 to 140°F)
Storage relative humidity: 20-90% RH
(non-condensing)
Noise level: 50 dBA or lower
(when properly balanced)
Instrument Life Expectancy:
7 Years.
Conditions: The safety precautions provided in the
operating manual and medical package insert must be
observed. The instrument needs to be regularly maintained
according to the instructions in Section 4, Care of the
Instrument.
NOTE: It is recommended that preventative maintenance
is performed on the instrument once a year.
1.5 Safety Standards
Complies with: UL 61010-1, 2nd:2004
UL61010-2-020:2006
CAN/CSA C22.2 No. 61010-1-04
CAN/CSA C22.2 No. 61010-2-20-09
CAN/CSA C22.2 No. 61010-2-101
IEC 61010-2-101
Safety Features:
Lid lock, Guard bowl monitor (imbalanced rotation detector),
Over-current protection, Over-heat protection

Page 7
CAUTION: The instrument is intended to be operated
indoors. Do not use it outdoors.
COMPARISION OF ROTATION SPEED RELATIVE
TO CENTRIFUGAL FORCE
Rotation speed
(rpm)
Relative
Centrifugal
Force (g) Approx.
Acceleration
(m/s2)
Approx.
200
5
46
300
11
104
400
19
184
500
29
288
600
42
414
700
58
564
800
75
737
900
95
932
1000
117
1151
1100
142
1393
1200
169
1658
1300
198
1946
1400
230
2256
1500
264
2590
1600
301
2947
1700
339
3327
1800
380
3730
1900
424
4156
2000
470
4605
2100
518
5077
2200
568
5572
2300
621
6090
2400
676
6631
2500
734
7195
NOTES:
・The distance from the center of the rotor to a
specimen chamber installed is 105 mm.
・Relative centrifugal force (RCF) = 11.739 x 10-5 x N2(g)
・N = Revolutions per minute (rpm)

1. INTRODUCTION
Page 8

2. INSTALLATION AND SETUP
Page 9
2.1 General Information
This section provides information on selecting a proper
location, unpacking, and installing the Cyto-Tek 2500
Cytocentrifuge. The instrument must be installed correctly
to ensure proper operation and service. Read this operating
manual carefully before attempting to operate the
instrument. Follow all instructions carefully.
The Cyto-Tek2500 Cytocentrifuge is a precision instrument
and must be handled accordingly. Rough handling or
dropping of the instrument will disturb or damage internal
components. Always handle the instrument with care.
2.2 Installation Requirements
The Cyto-Tek 2500 Cytocentrifuge will deliver optimum
performance if the following requirements are observed:
1. Place the cytocentrifuge on a strong, level surface
away from any induced mechanical vibration.
2. Do not subject the cytocentrifuge to direct sunlight or
any other form of heat source.
3. The instrument must be in an area of minimal contact
with volatile combustibles and corrosive gases.
4. The instrument should not be on the same electrical
circuit servicing equipment with motors, cooling units,
heating units, or light sources.
5. Mark a 30 cm (one foot) boundary around the
cytocentrifuge and permit no person or hazardous
material within this zone while the cytocentrifuge is
operating.
2.3 Unpacking
Open the carton. The carton will contain the following
accessories:
Accessories packed in the secondary accessory carton
include:
NOTE: An English explanatory material on the Japanese
warranty card and medical package insert is also included
for overseas customers.
If any of these items are missing contact Sakura Technical
Support (see Section 5.4).
If there is any visible shipping damage to any item,
immediately file a complaint with the carrier. Then notify
your Sakura instrument distributor.
2.4 Unpacking the Instrument
CAUTION: Use proper lifting techniques when
removing the instrument from packaging.
1. Cut the two plastic straps on the sides of the
carton.
2. Remove both cardboard spacers (Figure 2A) and
instrument lid gasket (Figure 2B).
Figure 2A
Description Quantity Check box
Power Cord
1
□
Paper Filter, 1mL
12
□
Chamber Holder, 1mL
12
□
Specimen Chamber, 1mL
12
□
Chamber Cap, 1mL
12
□
Operating Manual
1
□
Description Quantity Check box
Guard Bowl Unit
1 set
□
Medical Package Insert
(Japan Customers Only)
1 □
Warranty Card
1
□
Unpacking Instructions
1
□

2. INSTALLATION AND SETUP
Page 10
Figure 2B
3. Remove box tape.
4. Slightly hold up the instrument lid and remove the
foam support inserts (Figure 2C).
Figure 2C
5. Remove the top foam block insert (Figure 2D).
Figure 2D
6. Unwrap the proactive vinyl sheet from the
instrument and completely open the lid (Figure 2E).
Figure 2E
7. Remove adhesive tape that holds the guard bowl
unit in place and close the lid (Figure 2F).
Figure 2F
8. Grasp the bottom of the instrument and carefully
remove the instrument from the carton (Figure 2G).
Figure 2G
Guard bowl unit
Adhesive tape

Page 11
9. Open the lid by pressing the Lid Open button
(Figure 2H). Remove the guard bowl unit from the
instrument (Figure 2I).
Figure 2H
Figure 2I
10. Remove a foam cushion (Figure 2J).
Figure 2J
11. Install the lid gasket, see Section 4.1.3. (Figure 2K).
12. Replace the guard bowl into the instrument
(Figure 2K).
Figure 2K
Lid open push button
Guard bowl unit
Foam cushion
Lid gasket
Guard bowl unit

2. INSTALLATION AND SETUP
Page 12
2.5 Standard Accessories and
Options
2.5.1 Standard Accessories
NOTES:
・The cell deposition area of 1mL paper filter is 6 x 6 mm.
・The cell deposition area of 6mL paper filter and 6mL
rubber gasket is 14 x 14 mm.
・The cell deposition area of 12mL paper filter and 12 mL
rubber gasket is 14 x 30 mm.
2.5.2 Options/Consumables
2.5.3 Missing or Damaged Items?
Check the contents of all shipping cartons and boxes
carefully for all of the listed items. If any items are visibly
damaged, or are missing, call 1-800-725-8723 or 1-310-
972-7800 (U.S. Customers only). If you are located outside
the U.S., contact your nearest Sakura instrument distributor
Description/Product Code
Quantity
①
Power Cord (100/115V), A4010548
or
Power Cord (230V), A4010549
1
②
Guard Bowl Unit, F60-755-00
(Guard Bowl, Rotor, Bowl Lid)
1 set
1mL Specimen Chamber Kit, 4301
Includes the following items:
12 each
③
Specimen Chamber, 1mL
④
Chamber Holder, 1mL
⑤
Chamber Cap, 1mL
⑥
Paper Filter, 1mL
Operating Manual, 0007129-01
1
Medical Package Insert (Japan Only)
1
Description/ Product Code
1mL Specimen Chamber Kit, 4301
Includes the following 4 items:
③1mL Specimen Chamber
④1mL Chamber Holder
⑤1mL Chamber Cap
⑥1mL Paper Filter
⑦
6mL Specimen Chamber, 4331
⑧
12mL Specimen Chamber, 4328
⑨
6/12mL Chamber Holder, 4326
⑩
6/12mL Chamber Cap, 4334
⑪
6mL Rubber Gasket, 4327
⑫
12mL Rubber Gasket, 4337
①
②
③
④
⑤
⑥
③
⑨
⑩
⑩
⑦
⑨
⑪
⑫
④
⑤
⑥
⑧

3. OPERATING INSTRUCTIONS
Page 13
3.1 Operating Instructions
3.1.1 Workflow (Figure 3A)
The following is a basic operating flow when using the
Cyto-Tek 2500 Cytocentrifuge:
Figure 3A
3.2 Customization of Settings
3.2.1 Creating a Program
The instrument starts up in the standby mode after power-
on. The control panel displays the program number and
settings of a currently-selected program. The instrument
saves up to 30 programs. Selectable rotation speed is from 200
to 2500 rpm, and is displayed in multiples of 10 rpm.
NOTE: The factory default settings are 1,000 rpm and 10
minutes. Programs can be edited as desired (Figure 3B).
Figure 3B
Setting a Rotation Speed (Figure 3C)
Figure 3C
1. Select the program number, then press the SET key.
NOTE: The Speed display starts to flash, indicating that
the speed setting mode is up.
2. Determine the desired rotor speed for the program
cycle samples. Press the UP () and DOWN () keys
until the Speed Display indicates the desired rotor
speed. Selectable rotation speed is from 200 to 2500
rpm, and is displayed in multiples of 10 rpm. If these
keys are held down for one second or more, the value
will change by 50.
3. Press the SET key to save the changes and enter the
time (minutes) setting mode.
Assemble the specimen chambers, dispense specimen into each chamber and
install the chamber cap securely.
CAUTION: Use microscopic glass slides with ground edges.
CAUTION: Glass slides might break, depending on their size and quality.
Determine if glass slides you want to use can be safely assembled
in the chamber holders and processed on the instrument before
use.
Load the rotor in the guard bowl with the assembled chambers.
(Balance the rotor while loading).
Put on the bowl lid and lock it.
Open the instrument lid and set the guard bowl unit in place.
Close the instrument lid.
Select the program number of a program to run, using the UP (

) and DOWN
(

) keys. If a desired program does not exist, edit and save a new program.
Press the START/STOP key to start the operation.
The lid is locked and the lid lock indicator is lit.
After the lid lock indicator has shut off, open the instrument lid.
Remove the guard bowl unit and close the instrument lid.
Remove the bowl lid.
Remove the specimen chamber holder assemblies from the rotor.
Carefully remove the glass slide from the chamber holder.
Disinfect or sterilize the guard bowl, bowl lid, and others.
When the operation is ended, the completion buzzer sounds.

3. OPERATING INSTRUCTIONS
Page 14
NOTE: To return to the standby mode without saving the
change, press the START/STOP key.
CAUTION: The rotation speed is selectable from 200
rpm to 2,500 rpm. However, the morphology of cells
may be degraded if a higher rotation speed is
selected. Consider an appropriate value before
setting the speed.
Setting a Process Time (minutes) (Figure 3D)
Figure 3D
1. Press the SET key.
2. Press the UP () and DOWN () keys until the Time
Display indicates the desired time in minutes.
3. Press the SET key to save the changes and enter the
time (seconds) setting mode.
NOTE: To return to the standby mode without saving the
change, press the START/STOP key.
CAUTION: The process time is selectable from 0
minute and 1 second to 99 minutes and 59 seconds.
However, a prolonged process time may dry out
specimens. Consider an appropriate value before
setting the time.
Setting a Process Time (seconds) (Figure 3E)
Figure 3E
1. Press the SET key.
2. Press the UP () and DOWN () keys until the Time
Display indicates the desired time in seconds.
3. Press the SET key to save the changes.
NOTE: To return to the standby mode without saving the
change, press the START/STOP key.
Set and View Mode
When the SET key is held down for 3 seconds or more and
released, the instrument will start the set & view mode for
setting system options and viewing the software version
number and error logs.
This set & view mode provides the following functions:
•Selecting a completion buzzer
•Turning ON or OFF an alert for preventing slides
from drying
•Selecting the volume
•Viewing the software version number
•Viewing the error code of an error occurred
Selecting a Completion Alarm SET-1 (Figure 3F)
Figure 3F
1. In Standby mode, press the SET key for 3 seconds or
more. The completion buzzer setting mode displays
“SET-1” on the Speed display.
2. Use the UP () and DOWN () keys to change the
value in the Time display from 1 to 3. Each setting is
as follows:
1: (Pi--, Pi--, Pi--)
2: (Pi Pi, Pi Pi, Pi Pi)
3: (PiPiPi--, PiPiPi--, PiPiPi--)
3. To test the sound in the selected pattern, press the
START/STOP key. Press the key again to stop the
buzzer.
4. Press the SET key to save the change and enter the
alert On/Off setting mode.

Page 15
Turning an Alert ON or OFF SET-2 (Figure 3G)
Figure 3G
1. Press the SET key in the completion alarm setting
mode. The alert On/Off setting mode display “SET-2”
in the Speed display.
NOTE: Allows the operator to turn On/Off a End of
Process alarm to prevent slides from drying.
2. Use the UP () and DOWN () arrow keys once at
the end of a run to select “Off” to sound the completion
buzzer. Or select a desired time interval in one-second
increments (selectable from 0 second to 60 seconds) to
keep the completion buzzer sounding. The value is
displayed in the Time display, like “On00” or “On60”,
and circulated on every key press.
3. Press the SET key to save the change and enter
the sound volume setting mode.
Selecting a Sound Volume SET-3 (Figure 3H)
Figure 3H
1. Press the SET key in the alert On/Off setting mode.
The sound volume setting mode displays “SET-3” in the
Speed display.
NOTE: Three sound volume levels are available for the
completion and error alarms. Use the UP () and DOWN
() arrow keys to change the value in the Time display from
1 - 3. Each setting is as follows:
1: (Low)
2: (Middle)
3: (Loud)
2. To test the sound volume, press the START/STOP key.
Press the key again to stop the alarm.
3. Press the SET key to save the change and switch to the
software version number display.
Viewing the Software Version Number (Figure
3I)
Figure 3I
1. Press the SET key in the sound volume setting mode.
The version number of the software currently installed
in the instrument is displayed.
2. Press the SET key to switch to the error code display.
Viewing Error Codes (Figure 3J)
Figure 3J
Error Codes have been programmed into the Cyto-Tek 2500
Cytocentrifuge. These 2-digit codes alert the operator to a
possible instrument malfunction or operating error. They
are displayed in the Speed Display window. An alarm
buzzer sounds when an error code is displayed.
1. Press the SET key when the software version number
is displayed. The error code display appears.
2. The last three errors codes are listed.

3. OPERATING INSTRUCTIONS
Page 16
NOTE: The occurrence order of the errors is displayed in
the Program display. The error code of each error is
displayed in the Speed display. If there is no error stored in
memory, the Speed display will show “E---“.
3. Press the SET key to return to the standby mode.
3.3 Assembling the Specimen
Chamber and Dispensing the
Specimen
CAUTIONS:
•The 1mL specimen chamber, chamber holder,
chamber cap and paper filter are disposable.
Do not reuse them.
•Confirm that a name and ID on each glass slide
match those of individual patients or subject.
•The limit of rotating imbalance of load is 3
grams. Evenly fill the specimen chambers to be
placed in the rotor.
•Use the 1mL specimen chamber only with the
1mL chamber holder, chamber cap, and paper
filter. Those for the 6mL and 12mL chambers
are not compatible.
•When the paper filter is in use, cytocentrifuge
the chambers within 2 ~ 3 minutes of
dispensing the specimen.
•The 6mL and 12mL specimen chambers, rubber
gaskets, 6/12mL specimen chamber holder and
cap are reusable after being disinfected and
washed. However, if they have cracks or are
deformed, do not use them.
•Do not use glass slides that have flaws. They
will be broken during operation.
•After the specimen has been dispensed,
immediately lock the bowl lid and start the
operation.
•Do not place different types of the chamber
assemblies in the rotor at a time. Doing so will
cause an imbalance error to occur during
operation.
•Glass slides might break, depending on their
size and quality. Determine if glass slides you
want to use can be safely assembled in the
chamber holders and processed on the
instrument before use.
•If the specimen chamber using the rubber
gasket is overfilled, aerosoling will occur
inside the guard bowl. If it is under filled,
uneven and inadequate smearing will result.
Procedure for Using 1mL Disposable
Specimen Chambers
1. Write the name of the specimen on a clean glass
slide and place the slide, frosted side up, in the
specimen chamber holder (Figure 3K).
Figure 3K
2. Place the new paper filter on the slide (Figure 3L).
Figure 3L