Sakura Tissue-Tek Glas User manual

Coverslipping
Tissue-Tek® Glas™ Coverslipper
Quick Reference Guide
Tissue-Tek® Glas™ Coverslipper
Sakura Finetek Europe B.V. | Flemingweg 10a, 2408 AV Alphen a/d Rijn | NL
Phone: [phone] | Fax: [phone] | [e-mail] | www.sakura.eu
Start Up
The Tissue-Tek®GlasTM Coverslipper is a system that dispenses mounting medium onto stained
histopathological and cytopathological samples on glass slides as well as coverslipping these samples
using cover glasses.
Loading door
Adjustable
Feet level
Coverglass
holder
Available in 40,
50, 55 and 60
mm
Display Panel
Debris tray
Mounting medium
bottle
Receiving racks

Tissue-Tek® Glas™ Coverslipper
Coverslipping
Quick Reference Guide
Tissue-Tek® Glas™ Coverslipper
page 2 | 6 QRG | Glas Coverslipper | 2011 | V-2
Preparation before cover slipping
Hardware
Placing cover glasses
1. Put cover glass holder in place.
2. Make sure the tab of the cover glass holder fits
in the holder (check on the CG size).
Placing coverglasses
Placing mounting medium
When setting the mounting medium bottle for the
first time, you must fill the discharging channel
with mounting medium.
1. Place the waste bottle below the dispensing
nozzle.
2. Fill the priming bottle with mounting medium.
3. Connect the tip of the introduction tube with
the priming bottle.
4. Squeeze the priming bottle to feed the
mounting media until it reaches the dispensing
nozzle.
5. Remove the priming bottle from the
introduction tube.
6. Set the mounting medium bottle.
7. Tilt the mounting medium tube towards the
front and lift the tube.
8. In order to prevent air bubbles, insert the tube
gently into the bottle.
9. Perform an [initial priming] page 2.7 OM.
10. Press [EMER. STOP] to enter the set up
menu.
11. Highlight [1. Prime Dispenser].
12. Press [Verify].
13. Press [Start] and again [Start] to prime.
Changing mounting medium brands
Since mounting mediums vary in viscosity and
formulation, it will be necessary to follow the same
priming procedure when changing to a different
brand of mounting medium.
Follow the detailed instructions written in the
Operating Manual, “priming the instrument”
beginning on page 2.7.
Placing mounting medium Priming bottle connected
to introduction tube
Cover glass holder
Holder hole
Cover glasses
Introduction tube

Coverslipping
Tissue-Tek® Glas™ Coverslipper
Quick Reference Guide
Tissue-Tek® Glas™ Coverslipper
QRG | Glas Coverslipper | 2011 | V-2 page 3 | 6
Installing the nozzle reservoir
1. Set the dispensing tray in the system by
aligning the tray with the two guide rails (only
for the first time use).
Placing Anti-drying Bottle (1) and waste Container (2
Filling anti drying bottle
1. Fill the anti drying bottle with solvent to just
below the lid.
Note: type of solvent depends on the mounting medium
2. Place the anti-drying bottle and waste
container in the dispensing tray.
Handling the dispensing nozzle
1. When setting the anti-drying bottle, push down
the dispensing nozzle in a backward direction
and then set the bottle.
2. After the anti-drying bottle and waste container
have been set, lift the dispensing nozzle and
return the nozzle so that its tip enters the hole
in the anti-drying bottle. (see description
“handling the dispensing nozzle”).
Handling dispensing nozzle Filling anti-drying bottle
Filling “pump anti drying bottle”
1. Remove the lid and add solvent for mounting
medium into the pump reservoir.
Note: type of solvent depends on the mounting medium.
2. Fill the pump anti drying bottle with solvent to
just below the lid.
Filling pump anti drying bottle
Filling loading station
1. Fill the loading station with solvent until the
specified level.
Note: type of solvent depends on the mounting medium.
Filling loading Station
Required level
Required level
1
2

Tissue-Tek® Glas™ Coverslipper
Coverslipping
Quick Reference Guide
Tissue-Tek® Glas™ Coverslipper
page 4 | 6 QRG | Glas Coverslipper | 2011 | V-2
Preparation before coverslipping
Software
VOLUME KNOB
Can be used to fine tune the volume of mounting
medium dispensed on the slide.
SPEED KNOB
Refers to the speed at which the coverglass is
applied to the slide.
STOP
Can be used to stop the instrument while operating.
TEST
Can be beneficial if the operator would like to test
one slide.
SIZE
Toggles between coverglass sizes 40, 50, 55 and 60
mm.
VERIFY
Key acts as an enter key.
PRIMING FUNCTION
Can be used to verify proper dispensing.
When “single key”is pressed mounting medium
will be dispensed from the nozzle one time.
When “continuous”key is pressed the mounting
medium will dispensed continuously until the
“STOP” key is pressed.
Control panel
Coverslipping Operation
Cover slipping slides
Pre-operational checks
1. Turn on POWER.
2. Remove the loading station lid.
3. Check the solvent volume level in anti-drying
bottle and dispensing pump reservoir.
4. Clean any possibly spilled mounting medium.
5. Place receiving racks in the unloading area.
6. Put coverglass (CG) holders in place and fill
with coverglasses.
7. Check if CG size on screen matches CG in
holder.
8. Check the volume level of mounting medium.
Start of operation
1. Check CG size, coverslipping speed and volume
of mounting medium.
2. Select coverslipping program.
3. Press and hold [prime] key for 3 seconds
4. Pull out the loading station and set baskets.
Close loading station door and [start]
coverslipping.
Check CG size
Insert slides in parallel
Do not tilt slides
Loading of the slides
Set by making sure the
“UP SIDE” marks are
facing the right.
Loading of the baskets

Coverslipping
Tissue-Tek® Glas™ Coverslipper
Quick Reference Guide
Tissue-Tek® Glas™ Coverslipper
QRG | Glas Coverslipper | 2011 | V-2 page 5 | 6
Maintenance
(section 5 operating manual)
Daily
Station cleaning
1. Clean each station and its surrounding area.
2. Brush off each station, remove dust and/or
glass particles.
3. Using a cloth moistened with solvent for
mounting medium*, remove any mounting
medium attached to the stations.
Cover glass and Slide glass arm cleaning
1. Clean the suction pads using a cloth moistened
with 100% alcohol.
End of Operation
1. Remove receiving racks.
2. Remove empty baskets.
3. Place dispensing nozzle in the lubrication bottle
above the solvent (1)! If the instrument will not
be used for more than one week, place the
dispensing nozzle in the solvent (2) by pulling
the lever towards you.
Power off
1. Turn off power and place the lid on the loading
station.
Mounting medium nozzle cleaning
1. Clean the mounting medium nozzle by using a
cloth moistened with solvent for mounting
medium*.
2. At the end of every day before switching off the
machine, place dispensing nozzle in the anti-
drying bottle above the solvent!
Weekly
Waste container
1. Remove waste container and replace it with a
clean container.
2. Check the spatula part.
Soak the used waste container for 24 hours in
solvent for mounting medium*.
Cleaning the loading station
Lever
Station cleaning
Suction pad Slide glass arm
Suction pad coverglass arm
1Normal state
2Dispensing nozzle
down lever is pulled
2
1
Lever

Tissue-Tek® Glas™ Coverslipper
Coverslipping
Quick Reference Guide
Tissue-Tek® Glas™ Coverslipper
page 6 | 6 QRG | Glas Coverslipper | 2011 | V-2
6 Months
Anti-drying bottle
1. Change the solvent for mounting medium*in
the anti-drying bottle.
Cleaning the receiving racks
1. Soak the racks for 24 hours in solvent for
mounting medium*.
Instrument strainer
Open cover on the right side of the instrument and
remove the strainer cap. Use xylene soaked brush
to clean the filter. Replace the cap.
Yearly
1. Preventive maintenance by a Sakura
representative or a qualified service engineer.
Trouble shooting
(section 6 operating manual)
The trouble shooting part of the operating manual
is divided into three sections to explain how to
recover from conditions that may occur during
routine operation.
In the first section,
Troubleshooting Chart is provided, which identifies
possible conditions related to the electrical and
mechanical operations.
The second section,
Messages, explains specific messages that may be
displayed during routine operation in detail.
The third section,
Gives a detailed explanation of all error codes and
how to recover from them.
Operating manual section 6, trouble shooting
1. Change the solvent for mounting medium*in
the loading station.
*Solvent for mounting medium is the reagent which is compatible
with the mounting medium, Tissue clear when using Tissue Mount
and Xylene when using Glass coverslipping medium.
Waste container, spatula part