Sakura 6134 User manual

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LINK MANUAL / 連結設定方法
Link Kit of Prisma®and Film® ／ プリズマ®・フイルム®連結キット
MODEL ： 6134
Manufactured for:
Sakura Finetek Japan Co.,Ltd
1-8-2, Shin-ohashi, Koto-ku Tokyo 135-0007 Japan
Sakura Finetek Europe B.V.,
2408 AJ Alphen aan den Rijn, The Netherlands
Made in Japan

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Link Procedure for Tissue-Tek®Prisma®Automatic Stainer and Tissue-Tek®Film®Coverslipper
The following explains procedures for linking the Film® with the Prisma®, using the Link
System kit, and for setting up the link installation program on those instruments.
< Tissue-Tek®Film®Link System >
1. Safety Precautions
Follow instructions stated below to ensure safety and proper instrument operation.
Improper handling and/or operation may cause physical hazards and damages not only on
the instruments but on specimens to be processed.
Warning
*Observe safety precautions for the instruments to be linked.
*The instruments must be installed and linked by specially trained sales reps or service
personnel only.
*Disassembly and/or installation of the linked unit must be performed by specially
trained service personnel only.
*Do not transport and/or move the linked unit. Doing so will give significantly heavy
load to the unit, causing fall and/or damage of the unit.
*A bench or table must have enough strength capable of supporting instrument weight.
Specified weight of the instruments is a dry weight. In actuality, a stronger or table
may be necessary due to additional weight of reagents loaded on the instruments.
*Be sure to use specific slide baskets. Failure to do so will possibly break or damage
specimens to be processed.
Caution
*If acid or alkaline reagents are used on the Prisma®with the Film®, make sure to put
reservoir lids on those solution reservoirs or to remove those reservoirs from the Prisma®
after use. Some of components in the linked Film®are low in tolerance for acid and
alkaline.

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2. Contents of Link System Kit
This is a kit for mechanically and electrically linking the Prisma®with the Film®. By
linking the Prisma®to the Film®with this kit, a series of works from the staining to the
coverslipping can be automatically performed.
The Link System kit consists of following items:
(1) Installation bar : 1
(2) Hex socket head bolts (M5 x 15) : 5
(3) Spring washers (M5) : 5
(4) Plain washers (M5) : 5
(5) Communication cable : 1
(6) Compact Flash Card : 1 (Link installation program for the Prisma®
included)
(7) Basket positioning fixture : 1 set
Prior to linking two instruments, prepare following tools.
a. Phillips screwdriver, No. 2
b. Phillips screwdriver, No. 1
c. Socket driver, width across flat 5.5mm
d. Allen wrench, width across flat 4.0mm

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3. Installation
3-1. Installation space
Following are conditions necessary to install two instruments. For other particular
environmental factors, please refer to the operating manual for each instrument.
A bench of 1,900mm or wider is necessary to install the linked unit on it. Also, a clearance
of 400mm or more should be provided between the right side of the Prisma®and a wall or
something to allow the control panel of the Prisma®to move properly. On the other side of
the link unit, a clearance of 450mm or more is needed to fully open the film door of the
Film®.
The bench where the linked unit is installed on must withstand a load of at least 3 kN.
3-2. Installation of Tissue-Tek®Prisma®
Install the Prisma®first.
(1) To remove link cover located at the left side of the Prisma®:
Use Phillips screwdriver No. 1 and socket driver to remove
cap nut and truss head screw at the top of link cover. Pull
link cover straight toward you a little until you can detach 2
white clips from the bottom. Then, while lifting link cover
up, draw it outward
(2) To attach installation bar to the Prisma®:
Hold installation bar in place and tighten 3 hex socket head
bolts (combined with plain and spring washers) using Allen
wrench.

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(3) Connect communication cable to the Prisma®in advance.
Now, the Prisma®is ready for linking with the Film®.
If an installation place does not allow easy access to the back side of the Prisma®, the
exhaust duct and the drain hose should be secured prior to linking with the Film®.
3-3. Installation of Tissue-Tek®Film®
To remove link cover located at the right side of the Film®:
(1) Open loading door. Using Phillips screwdriver No. 2,
remove a screw that secure link cover to instrument
base.
(2) Remove link cover from the instrument.
3-4. Link
(1) Place the Film®on the left-hand side of the Prisma®,
and then put the right edge of instrument base of the
Film®on installation bar attached to the Prisma®.

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(2) Turn adjustable feet of the Film®to make it level
with the Prisma®. Then, fix the Film®on
installation bar, using 2 hex socket head bolts (with
two washers combined), accessing from the Prisma®
side.
(3) Connect communication cable to the Film®.
4. Setting up Tissue-Tek®Prisma®
4-1. Installing the link installation program on the Prisma®
(1) Insert the Compact Flash Card into the slot located
at the left side of the control panel of the Prisma®.
(2) Turn on power switch. The link installation
program will start.

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(3) The initial screen appears. Then, gently touch
the display screen.
(4) The initial screen changes to the menu screen.
Three buttons are displayed on the screen.
The link installation program enables you
To install the link information on the
Prisma®(*),
To set the basket transfer position in the
Prisma®, and
To check the basket transfer by the Prisma®.
(*) The link information is not installed on the standard version of the Prisma®. Without
the link information installed, the Prisma®cannot recognize the Film®.
4-2. Installing the link information on the Prisma®
(1) Touch the first “Install the Link Information”
button on the menu screen.
(2) Touch the “Install/Uninstall” button in the
“Install the Link Information” screen to change
the indication in the “Prisma Link Status” field
to “Link to Coverslipper” from “None Link”.
When “Link to Coverslipper ” was displayed (*),
the link information has been installed on the
Prisma®.

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(*) Touching the “Install/Uninstall” button again will set the “Prisma Link Status” back to
“None Link”. When the “None Link” is on, the link information is in the uninstalled
status.
(3) Touch the “Return” button to return to the menu screen.
5. Setting the Basket Transfer Position
After the link information was installed, follow the steps below to set the basket transfer
position for the Prisma®.
(1) Move the loading container to the Prisma®from the Film®.
(2) Place the basket positioning fixture into the loading container.
(3) Set the basket transfer position through the link position setting menu in the link
installation program.
(4) Remove the basket positioning fixture and return the loading container to the Film®.
5-1. Moving the loading container to the Prisma®
(1) Hold the MENU key pressed and turn power
switch on. The Film®displays the main menu
screen. Keep the MENU key pressed for at
least 3 seconds.
(2) Move the cursor to “3. Adjustment of link” in the
main menu screen, using the right arrow key.
Then, press the ENTER key. The “Adjustment
of Link” screen appears.

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(3) Move the cursor to “2. Adjustment” in the
“Adjustment of Link” screen, using the right
arrow key. Press the ENTER key.
(4) When the next screen was displayed, press the
ENTER key. Then, the return home movement
is carried out.
(5) After the next screen appeared, place the loading
container into the Film® and press the START
key. The loading container starts moving
rightward and stops just before it goes into the
Prisma®.
(6) The Y-axis offset value is displayed on the screen.
Press the ENTER key. The loading container
moves into the Prisma®.

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(*) Back-and-forth movement (Y-axis) is adjustable through this screen. However, it is
unnecessary to adjust it again because it has been already done before factory shipment.
(7) The screen displays the X-axis offset value.
The Film®is ready.
(*) Right-and-left movement (X-axis) is adjustable
through this screen. If the loading container
interferes with the exterior or the sink in the
Prisma®, the alignment of both instruments
needs to be adjusted.
5-2. Placing the basket positioning fixture
Place the basket positioning fixture into the loading container so that notches of the fixture
can be fitted into the basket receiver in the container, as shown below.

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5-3. Setting the basket transfer position
Initiate the link installation program in the Prisma®(see the paragraph 4-1). Set the
position where baskets are handed over to the Film®through the transfer position setting
function in the Prisma®.
(1) Touch the second “Link Position Setting” button on the menu screen in the link
installation program.
(2) The “Move” dialog appears. Pressing the “ON”
button initiates the return-home movement
before the robotic arm starts moving to above the
loading container.
(3) When the robotic arm is in motion, the “Running”
dialog is displayed. Please wait a minute.
(4) When the robotic arm stopped, the “To Film Offset”
screen appears. The screen displays 3 buttons.
To read three dimensional coordinates the arm is
currently positioned, press the “SET” button. To
save information on the arm position read by the

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“SAVE” button, press the “SAVE” button. To return to the menu screen without saving
the information, Press the “BACK” button.
(5) Hold the robotic arm on the hook portion by a hand
and slowly move it down toward the top of the basket
positioning fixture in the loading container.
(6) Fit the groove of the center hook on the robotic arm into
the notch on the top of the basket positioning fixture.
Do not force to fit the groove into the notch. Doing so
may get the fixture shifted. If they are not aligned,
adjust them by moving the arm to X, Y, and Z directions
respectively.
(7) Press the “SET” button to read the offset value of
each axis and to display readings.
If “UNDER” or “OVER” is displayed in the fields,
the arm is possibly far out of position. Check the
fixture, the loading container, the Prisma®and/or
the Film®to see if they are properly aligned with
each other.

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(8) After the basket transfer position was determined,
press the “SAVE” button to save the offset values
into instrument memory and also to return to the
menu screen.
<Reference>
How to read offset values
As shown on the right, the offset
value is defined according to the
direction the robotic arm moves.
Each offset value varies in
increments of the following distance.
* X-axis: 0.1 mm
* Y-axis: 0.05 mm
* Z-axis: 0.05 mm
5-4. Lifting up the robotic arm
(1) After the basket transfer position was set, gently move the robotic arm up while holding
the hook portion by a hand to escape the arm from the area the loading container
moves.
(2) Remove the basket positioning fixture from the loading container.
(3) Press the ENTER key on the control panel of the Film®to return the loading container
to the Film®. After the container reached its place in the Film®, turn off instrument
power.

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6. Verifying the Basket Transfer
Perform the basket transfer through the communication between two instruments to see if
baskets are properly delivered to the Film® from the Prisma®. This should be done with
empty slide baskets first and then, if the first run is successfully finished, perform the second
run with slide-loaded baskets.
6-1. Preparation
Turn on the power of the Film®. Prepare the Film® for normal use; that is, install the
xylene bottle and the coverslipping film on the instrument, and verify that the instrument
can work properly.
Prepare 3 20-slide baskets and 3 20-slide basket adapters.
6-2. Verifying the basket transfer
(1) Touch the last “Confirm link to Coverslipper”
button on the menu screen in the link installation
program.
(2) The “Confirm link to Coverslipper ” dialog is
displayed. Close the cover of the Prisma®before
touching the “START” button on the screen.
The Prisma®demands the Film®to deliver the
loading container to the Prisma®through
communication between them. The
“Coverslipper Preparing” dialog is on the screen
until the loading container is send out of the
Film®.

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(3) After the Film®delivers the loading container to the
Prisma®, the dialog shown on the right appears on the
screen.
(4) Open the door. Place 3 slide baskets prepared in
advance into the reservoir located at the far right.
Face the “UP SIDE” mark on each slide basket
toward the right when placing into the reservoir.
(5) Touch the “OK” button.
(6) After completion of the return-home movement, the
robotic arm goes and picks up slide baskets from the
start station. The robotic arm transfers baskets to the
loading container. After releasing them into the loading
container, the arm returns to its home position. After
the arm stopped at its home, the Film®returns the
loading container to its original position and begins
the coverslipping operation. When the Prisma®is in
operation, the “Running” dialog is on the screen.
(7) When the first “Confirm link to Coverslipper” screen
appeared, touch the “BACK” button to return to the
menu screen.
Were the baskets properly transferred to the Film®? If not, adjust the basket transfer
position again.

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7. Handling of the linked Prisma®
This section explains the information necessary to actually use the linked Prisma®. Please
also refer to each operation manual of Tissue-Tek®Prisma®and Tissue-Tek®Film®.
*To send stained-slide baskets to the Film®during operation, see “7-1 Changing the
system setup.”
*To know how the linked Prisma®is operating, see “7-2 Staining process when the link is
used.”
7-1. Changing the system setup
The Prisma®allows selecting whether or not the stained-slide baskets are transferred to the
Film®. To transfer them to the Film®, it is necessary to change settings in the Utility
Menu.
(1) Select “System Setup” in the Utility Menu.
(2) Touch the button for “Coverslipper Link”
to change “Not in Use” to “In Use”.
Note:
When the link information is not installed on
the Prisma®by the link installation program,
the button for “Coverslipper Link” is not
displayed.
If you do not want to transfer baskets to the
Film®, although it is linked, select “Not in Use”.

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(3) The link station (LK) is displayed at the left
bottom corner in the Stain Process monitor
screen.
7-2. Stain process when the link is used.
When the link is used, the slide baskets are carried not to the end station but to the link station
after completion of stain process. However, when the loading container is in use, the
stained-slide baskets are carried to the end station to wait until they can be transferred to the
Film®. When the Film®is ready to receive the baskets, the Prisma®carries them to the link
station from the end station in an interval between other staining runs.
Note:
When the stained-slide baskets were brought to the end station, you can remove them from the
Prisma®by touching the “Remove” key.
Note:
If you want to run a staining program that does not need the coverslipping by the Film®, assign
the last of that program to the program end station (PE) so that you can remove the stained-slide
baskets from the Prisma®.
When the power is not supplied to the Film®
When the link station is displayed but the power is not supplied to the Film®, the dialog of Error
800 is generated.

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To continue the operation, touch the “YES” button and prepare the Film®to begin the
coverslipping. To stop the operation, touch the “NO” button. The stained-slide baskets are
placed into the end station.
If you want to resume the link operation after you stopped it by selecting the NO button, follow
the steps below.
(1) Touch the “Menu” button at the right
bottom of the Stain Process Monitor
screen to access the Stain Process Menu
screen. Then, touch the “Staining Mode
Selection” button.
(2) Touch the button for the “Link with
Coverslipper” setting to change it to “YES”
from “NO”.

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「Tissu-Tek®Film®」と「Tissue-Tek®Prisma®」の連結設定方法
「Tissue-Tek® Film® 連結用キット」
１． 本製品をご使用になる前に
本製品を正しくお使いいただくために次のことをお守りください。誤った使い方をすると危険であ
り、装置だけでなく、標本を破損、損傷することがあります。
警告
連結を行う装置の安全上の注意事項を守ってください。
本製品は、専門の訓練を受けた営業マン、又はサービスマンなどによって連結・設置を行っ
てください。
連結後の分解・設置は、専門の訓練を受けたサービスマンなどによって行ってください。
連結状態での装置の運搬・移動は、行わないでください。連結状態での運搬・移動は、製品
に著しく大きな力を与えることがあり、製品の落下、破損の原因になります。
テーブル等の強度が、装置の重量に耐えるものをご利用ください。仕様での重量は、乾燥重
量を示しています。実際には、お使いになる薬液等により、より高い強度のテーブル等が必
要になる場合があります。
必ず、指定の標本用バスケットをご利用ください。指定以外のバスケットを使用した場合、
標本の破損、損傷を招く恐れがあります。
注意
Tissue-Tek® Prisma®で酸性、又はアルカリ性の薬液を使用する場合は、使用後に必ず、各
種の薬液容器蓋をするか、薬液を装置から取り外してください。連結する封入装置には、酸
性やアルカリ性に弱い部分があります。

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２． Tissue-Tek®Film®連結用キットとは
本キットは、フィルム式自動封入装置 Tissue-Tek®Film®と自動染色装置 Tissue-Tek®Prisma®を
機械的、電気的に連結を行うためのキットです。この連結キットで連結することにより、標本の染
色から封入までの一連の作業を自動化することが出来ます。
Tissue-Tek® Film®連結用キットの構成は下記の通りです。
①連結バー ： 1 個
②六角穴付きボルト（M5×15） ： 5 個
③バネ座金 (M5 用) ： 5 個
④平座金（M5 用） ： 5 個
⑤装置間通信ケーブル ： 1 本
⑥コンパクトフラッシュカード ： 1 個
（Prisma 用インストールプログラム内臓）
⑦位置決め治具 ： 1 セット
なお、連結を行う際に下記の工具をご用意ください。
A.プラスドライバー ： No.2
B.プラスドライバー ： No.1
C.ソケットドライバー ： 対辺 5.5 ｍｍ
D.六角レンチ ： 対辺 4 ｍｍ