Sartorius Vivaspin 500 User manual

Instructions for Use
Vivaspin®500 and 2
Centrifugal Ultrafiltration Units for General Laboratory Use
3104647-001-00

Contents
Instructions for use Vivaspin®500 | 2 3
Contents
1 About these Instructions.................................................5
1.1 Scope .................................................................5
1.2 Target Groups.........................................................7
1.3 Symbols Used.........................................................7
1.3.1 Warnings in Operation Descriptions...........................7
1.3.2 Other Symbols.................................................7
2 Safety Instructions .......................................................8
2.1 General Functions ....................................................8
2.2 Personnel Qualification ...............................................9
2.3 Significance of these Instructions.....................................9
2.4 Functionality of the Product ..........................................9
3 Product Description ....................................................10
3.1 Vivaspin®500........................................................10
3.2 Vivaspin®2 ............................................................11
3.3 Product Symbols .....................................................12
4 Process Preparation.....................................................13
4.1 Scope of Delivery ....................................................13
4.2 Unpacking ...........................................................13
5 Operation...............................................................14
5.1 Pre-Rinsing the Product .............................................14
5.2 Sanitizing the Product ...............................................14
5.3 Applying the Sample.................................................15
5.4 Inserting the Product into the Centrifuge............................16
5.5 Performing Filtration.................................................16
5.6 Removing the Sample................................................16
5.7 Vivaspin®2 ...........................................................17
5.7.1 Removing the Concentrator from the Filtrate Container .....17
5.7.2 Reverse spin with Vivaspin®2 .................................18
5.8 Desalting or Buffer Exchange ........................................18
6 Storage .................................................................19
6.1 Storing the Product ..................................................19

Contents
4 Instructions for use Vivaspin®500 | 2
7 Disposal................................................................20
7.1 Decontaminating the Product ......................................20
7.2 Disposing of the Product............................................20
8 Technical Specifications ................................................21
8.1 Dimensions ..........................................................21
8.2 Materials .............................................................21
8.3 Ambient Conditions..................................................22
8.4 Operating conditions ................................................22
8.4.1 Filtration Volumes ............................................22
8.4.2 Centrifugation Limit Values...................................23
8.5 Equipment Required.................................................23
8.5.1 Pipettes.......................................................23
8.5.2 Centrifuges ...................................................24
8.6 Sanitizing Methods ..................................................24
9 Typical Performance Characteristics....................................25
9.1 Vivaspin®500....................................................... 25
9.2 Vivaspin®2 .......................................................... 26
10 Chemical Compatibility.................................................27

Instructions for use Vivaspin®500 | 2 5
About these Instructions
1 About these Instructions
1.1 Scope
These instructions are part of the product. These instructions apply to the
following versions of the product:
Vivaspin®500 PES Quantity Prod. no.
3 kDa 25 | 100 VS0191 | VS0192
5 kDa 25 | 100 VS0111 | VS0112
10 kDa 25 | 100 VS0101 | VS0102
30 kDa 25 | 100 VS0121 | VS0122
50 kDa 25 | 100 VS0131 | VS0132
100 kDa 25 | 100 VS0141 | VS0142
300 kDa 25 | 100 VS0151 | VS0152
1,000 kDa 25 | 100 VS0161 | VS0162
0.2 μm 25 | 100 VS0171 | VS0172

6 Instructions for use Vivaspin®500 | 2
About these Instructions
Vivaspin®2 PES Quantity Prod. no.
3 kDa 25 | 100 VS0291 | VS0292
5 kDa 25 | 100 VS0211 | VS0212
10 kDa 25 | 100 VS0201 | VS0202
30 kDa 25 | 100 VS0221 | VS0222
50 kDa 25 | 100 VS0231 | VS0232
100 kDa 25 | 100 VS0241 | VS0242
300 kDa 25 | 100 VS0251 | VS0252
1,000 kDa 25 | 100 VS0261 | VS0262
0.2 μm 25 | 100 VS0271 | VS0272
Vivaspin®2 Cellulose
Triacetate Quantity Prod. no.
10 kDa 25 | 100 VS02V1 | VS02V2
20 kDa 25 | 100 VS02X1 | VS02X2
Vivaspin®2 Hydrosart®Quantity Prod. no.
2 kDa 25 | 100 VS02H91 | VS02H92
5 kDa 25 | 100 VS02H11 | VS02H12
10 kDa 25 | 100 VS02H01 | VS02H02
30 kDa 25 | 100 VS02H21 | VS02H22

Instructions for use Vivaspin®500 | 2 7
About these Instructions
1.2 Target Groups
The instructions are designed for the following target groups. The target
groups must possess the knowledge listed below.
Target Group Knowledge and Qualifications
Operator The operator of the product is responsible for compliance
with safety requirements and workplace safety regula-
tions. The operator must ensure that anyone working with
the product has access to the relevant information and is
trained to work with the product.
1.3 Symbols Used
1.3.1 Warnings in Operation Descriptions
NOTICE
Denotes a hazard that may result in property damage if it is not avoided.
1.3.2 Other Symbols
t Required action: Describes actions that must be carried out.
The actions in the sequence must be carried out in succession.
y Result: Describes the result of the actions carried out.

8 Instructions for use Vivaspin®500 | 2
Safety Instructions
2 Safety Instructions
2.1 General Functions
The product is intended for the ultrafiltration and | or diafiltration of biological
and aqueous solutions. The sample solutions and volumes used must be
suitable for the product.
The filtration process must be carried out in a centrifuge. Macromolecules
that are sufficiently larger than the nominal pore size of the membrane are
retained above the membrane and progressively concentrated. The vertical
membrane inhibits membrane fouling while the built-in dead stop impedes
concentration to dryness and loss of sample.
The product is supplied non-sterile. It is intended for single use and must be
disposed of after one use.
The product is intended exclusively for use in accordance with these instruc-
tions. Any further use beyond this is considered improper.
Operating Conditions for the Product
The product is intended for general laboratory use.
The product may only be used with the equipment and under the operating
conditions described in the Technical Data section of these instructions.

Instructions for use Vivaspin®500 | 2 9
Safety Instructions
2.2 Personnel Qualification
Persons without sufficient knowledge in the safe use of the device can injure
themselves and others.
If a specific qualification is required for an activity:The target group is indi-
cated. If no qualification is specified: The activity can be carried out by the
target group “Operator”.
2.3 Significance of these Instructions
Failure to follow the instructions might have serious consequences, e.g.
danger to individuals.
tRead the instructions carefully and completely. The instructions for action
build on each other.
tEnsure that the information contained in these instructions is available to
all individuals working with the product.
2.4 Functionality of the Product
A damaged product or worn parts can lead to malfunctions or cause hazards
which are difficult to identify.
tOnly operate the product when it is safe and in perfect working order.

10 Instructions for use Vivaspin®500 | 2
Product Description
3 Product Description
3.1 Vivaspin®500
1
2
3
Fig.1: Product overview (example)
Pos. Description
1 Concentrator
2 Membrane
3 Filtrate container

Instructions for use Vivaspin®500 | 2 11
Product Description
3.2 Vivaspin®2
1
2
3
4
Fig.2: Product overview (example)
Pos. Description
1 Concentrator cap
2 Concentrator
3 Membrane
4 Filtrate container

12 Instructions for use Vivaspin®500 | 2
Product Description
3.3 Product Symbols
Symbol Definition
Catalogue number
Do not reuse
Use by
Batch code
Manufacturer
Temperature limitation
Non-sterile product

Instructions for use Vivaspin®500 | 2 13
Process Preparation
4 Process Preparation
4.1 Scope of Delivery
Article Qty
Product packed in a cardboard box
Vivaspin®500 25 or 100
Vivaspin®2 25 or 100
Instructions for Use 1
4.2 Unpacking
Procedure
tNOTICE Risk of product malfunctions due to exceeding the usability!
Check the usability of the product (see specification on packaging).
Dispose of products forwhich the usability has been exceeded.
tUnpack the product.

14 Instructions for use Vivaspin®500 | 2
Operation
5 Operation
5.1 Pre-Rinsing the Product
Membranes in the product may contain traces of glycerin. If this substance
can interfere with the analysis of the sample: The membrane may be rinsed
before filtration.
Procedure
tRemove the concentrator cap.
tUse a pipette to apply a filling volume of buffer solution or deionized water
into the concentrator.
tReplace the concentrator cap.
tWash the buffer solution or deionized water through the membrane by
centrifugation.
tEmpty the concentrator and filtrate container.
tIf the pre-rinsed product is not used immediately: Cover the surface of the
membrane with buffer solution or water and store the product in the
refrigerator. The membrane must not dry out.
5.2 Sanitizing the Product
The product can be sanitized before use. The sanitizing method must be
suitable for the product (see Chapter "8.6 Sanitizing Methods," page 24).
Procedure
tRemove the concentrator cap.
tSanitize the product using the desired sanitizing method.
tEmpty the product.

Instructions for use Vivaspin®500 | 2 15
Operation
5.3 Applying the Sample
It is recommended that a pipette is used to apply the sample into the product.
The pipette must be compatible with the product (see Chapter "8.5.1
Pipettes," page 23).
Please ensure that the molecular weight cut-off (MWCO) of the product is
suitable for the size of the target molecule to be concentrated. In order to
ensure maximum recovery of the target molecule, it is recommended to
select a MWCO that is at least 50% below the size of the target molecule.
NOTICE
Risk of product malfunctions due to using unsuitable samples!
tOnly pour suitable samples into the product (see Chapter "10 Chemical
Compatibility," page 27).
NOTICE
Risk of product malfunctions or damage to the centrifuge due to exceeding
the maximum filling volume!
tDo not exceed the maximum filling volume (see Chapter "8.4.1 Filtration
Volumes," page 22).
Procedure
tCheck whether the MWCO of the product is suitable for the application.
tRemove the concentrator cap.
tApply the sample into the product using a pipette. Comply with the
maximum filling volume.
tReplace the concentrator cap.

16 Instructions for use Vivaspin®500 | 2
Operation
5.4 Inserting the Product into the Centrifuge
Procedure
tNOTICE Risk of product malfunctions or damage to the centrifuge! Only
use the product in suitable centrifuges (see Chapter "8.5.2 Centrifuges,"
page 24).
tInsert the product into the centrifuge.
tIf a centrifuge with fixed-angle rotor is used: Place the product into the
centrifuge so that the printed volume graduations on the concentrator are
facing upwards.
5.5 Performing Filtration
Procedure
tNOTICE Risk of product malfunctions or damage to the centrifuge.
Complywith the approved centrifugation limit values (see Chapter "8.4.2
Centrifugation Limit Values," page 23).
tCentrifuge the product in the centrifuge until the desired concentration
level is achieved.
5.6 Removing the Sample
Procedure
tIf the filtration or concentration is complete: Remove the product from the
centrifuge.
tRemove the concentrator cap.
tRecover the sample from dead stop pocket of the concentrator using
apipette.
tIf the membrane was pre-rinsed before filtration: Decant the filtrate and
concentrate.

Instructions for use Vivaspin®500 | 2 17
OperationOperation
5.7 Vivaspin®2
5.7.1 Removing the Concentrator from the
Filtrate Container
Procedure
tTo release the tube from the concentrator,
pinch the tube – to press it into an oval shape
– before removing it with a twisting action.
Press
here Press
here
Tube Sleeve

18 Instructions for use Vivaspin®500 | 2
OperationOperation
5.7.2 Reverse spin with Vivaspin®2
The concentrate can be collected into the
concentrator cap by reverse spinning, instead of
using a pipette.
Procedure
tRemove filtrate container.
tWith the concentrator cap still fitted, invert
the concentrator.
tInsert concentrator cap into filtrate container.
tInsert the product into the centrifuge.
tIf a centrifuge with fixed-angle rotor is used:
Place the product into the centrifuge so that
the printed volume graduations on the
concentrator are facing upwards.
tSpin at up to 3,000 gfor 2 minutes.
5.8 Desalting or Buffer Exchange
Procedure
tConcentrate the sample to the desired level.
tRemove the concentrator cap.
tDiscard the filtrate.
tRefill the concentratorwith an appropriate
exchange buffer.
tConcentrate the sample again.
tRepeat the process until the original buffer
and | or contaminating microsolute has been
sufficiently removed.
tIf the desalting or buffer exchange is
complete: Recover the sample.
Concentrator
Concen-
trator cap
Concentrate
Filtrate
container Filtrate

Instructions for use Vivaspin®500 | 2 19
Storage
6 Storage
6.1 Storing the Product
If the product has been unpacked and membrane has been pre-rinsed:
The membrane must be protected against drying out. For this purpose, the
membrane must be stored in a moist and cool condition.
NOTICE
Risk of damage to the product due to improper storage!
tComplywith the storage specifications.
Procedure
tIf the product is packaged: Store the product in the packaging.
tIf the product has been unpacked and the membrane has been
pre-rinsed:
tRemove the concentrator cap.
tCover the membrane with buffer solution orwater.
tReplace the concentrator cap.
tStore the product according to the ambient conditions (see Chapter "8.3
Ambient Conditions," page 22).

20 Instructions for use Vivaspin®500 | 2
Disposal
7 Disposal
7.1 Decontaminating the Product
If the product has come into contact with hazardous substances: Steps must
be taken to ensure proper decontamination and declaration. The operator of
the product is responsible for adhering to local government regulations on
the proper decontamination and declaration for transport and disposal.
Procedure
tIf the product has come into contact with hazardous substances:
Decontaminate the product.
7.2 Disposing of the Product
The product must be disposed of properly. The packaging is made of environ-
mentally friendly materials that can be used as secondary raw materials.
Requirements
The product must be decontaminated.
Procedure
tDispose of the product in accordance with local government regulations.
tDispose of the packaging in accordance with local government regula-
tions.