Sartorius PICUS User manual

User Manual
Picus® & Picus® NxT Electronic Pipette

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Table of Contents
1. Introduction 3
1.1. Intended Use 4
1.2. Product Overview 4
1.2.1. Single and Multichannel Pipettes 4
1.2.2. Display 5
1.2.3. Optifit Tips and SafetySpace Filter Tips 5
1.3. Contents of Delivery Package 6
2. Getting Started 7
2.1. Charging 7
2.2. Power Up 7
3. Operation 8
3.1. Operating Principles 8
3.2. Pipetting Modes 9
3.2.1. Pipetting (Forward Pipetting) 10
3.2.2. Reverse Pipetting 10
3.2.3. Multi-Dispensing 11
3.2.4. Manual Pipetting 12
3.2.5. Diluting 13
3.2.6. Sequential Dispensing 13
3.2.7. Multi-Aspirating 14
3.2.8. Titration 15
3.2.9. Protocols (only in Picus® NxT) 16
3.2.10. Advanced Functions 17
3.3. Saving Pipetting Programs to Memory 20
3.4. Setup 21
3.4.1. Adjustment 21
3.4.2. Sound 23
3.4.3. Backlight 23
3.4.4. User ID 24
3.4.5. GLP Info (only in Picus®) 24
3.4.6. Reset 25
3.4.7. Information 25
3.4.8. Languages 25
3.4.9. Pipette Lock 26
3.4.10. Passwords (only in Picus® NxT) 26
3.4.11. Date and Time (only in Picus® NxT) 27
3.4.12. Reminders (only in Picus® NxT) 28
4. Care and Maintenance 30
4.1. Cleaning and Maintenance 30
4.1.1. Single-Channel Pipettes 30
4.1.2. Multichannel Pipettes 33
4.2. Sterilizing 33
4.2.1. Autoclaving 33
4.2.2. UV Sterilization 33
4.3. Performance Testing 33
4.4. Replacing the Battery 35
4.5. Replacement Parts 35
4.6. Storage 35
5. Warranty 35
6. Hazardous Substances (RoHS II) 36
7. Disposal (WEEE) 36
8. Troubleshooting 36
8.1. Hardware Reset 36
8.2. Troubleshooting Guide 36
9. Technical Data 37
9.1. Performance Specifications 38
9.2. Speed Table 38
10. Ordering Information 40

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1. Introduction
The Sartorius Picus®/Picus® NxT electronic pipette brings ergonomics to
a completely new level with its exceptionally lightweight and compact
design, and its ease of operation. This versatile air displacement pipette has
been designed by an experienced R&D team in cooperation with laboratory
personnel and ergonomists to ensure safe and comfortable pipetting.
The Picus®/Picus® NxT is available in single and multichannel models (8 and
12-channel). Tips can be attached and ejected safely and comfortably using
the Optiload tip-loading feature and the electronic tip ejection function. To
improve safety by reducing the risk of contamination, replaceable Safe-Cone
Filters can be used in all Picus®/Picus® NxT models greater than 10 µl.
The fully electronic operation, unique DC motor, and the electronic brake and
piston control system guarantee excellent accuracy and precision. The desired
volume can be quickly selected using the light and easy-to-use adjustment
wheel on top of the pipette.
Picus® NxT offers advanced safety and efficiency features for highly regulated
laboratories. These features include a certificate of accredited 3-point
calibration, user-definable pipetting protocols, and calibration and service
reminders.
Congratulations on becoming a new Picus®/Picus® NxT owner!

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1.1. Intended Use
The Picus®/Picus® NxT pipette is intended, designed and manufactured for
dispensing liquids in a variety of applications and to be used in combination
with Optifit Tips or SafetySpace Filter Tips. The aforementioned pipette and tip
combination, fall within the scope of in-vitro diagnostics, and can be used as
a diagnostic medical device in related applications. Thereby, Picus®/Picus® NxT
and its accessories fulfil the relevant demands of the Directive 98/97/EC of the
European Parliament.
The Picus®/Picus® NxT product range covers a volume range of 0,2 µl to
10 ml. It is recommended that Sartorius Optifit Tips or SafetySpace Filter
Tips are used with Sartorius pipettes to ensure optimum compatibility and
performance.
It is a general purpose laboratory device that fulfils ISO 9001 and ISO 13485
standards.
This manual contains useful information, including information on good
pipetting practice.
NOTE: Prolonged pipetting can cause Work Related Upper Limb Disorder
(WRULD). The manufacturer is not responsible for WRULD or any related
injuries caused by using pipettes.
1.2. Product Overview
Before using the Sartorius Picus®/Picus® NxT electronic pipette for the first
time, please review this operating manual carefully. This manual can be
downloaded from the Sartorius website at: www.sartorius.com. Hardcopies
may be ordered by e-mail from [email protected].
1.2.1. Single and Multichannel Pipettes
1. Electronic tip ejector
2. Charging contacts
3. ON/OFF button
4. USB-charging port
5. Operating button & volume range colour-code
6. Adjustment wheel
7. Hotkey for memory places (black in Picus® NxT)
8. Softkeys for programming (black in Picus® NxT)
9. Dot-matrix display
10. Maximum volume
11. Dispensing head (tip ejector collar and tip-cone), autoclavable
(excl. 8/12-ch 1200 µl)
12. Optiload, spring-loaded tip-cones in multichannel pipettes
13. Safe-Cone Filters (excl. <10 µl pipettes)
14. Pipette tip
15. Tip ejector collar. (When using non-Sartorius tips, it can be adjusted for
suitable tip ejection, in 10µl, 120µl, 300µl and 1000µl single-channel
models)

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SafetySpace
air gap
]
1.2.2 Display
The multi-colour backlit dot-matrix display is clear and is logically structured.
1. Softkey functions
2. Current mode
3. Pipetting volume and aliquots in multidispensing modes
4. Speed
5. Battery charging indicator
6. Arrows indicating aspiration and dispensing
1.2.3 Optifit Tips and SafetySpace Filter Tips
We recommend using Sartorius Optifit Tips or SafetySpace Filter Tips.
These tips are designed for Sartorius pipettes and therefore ensure optimal
compatibility, accuracy, and precision.
For optimum results you should also:
−Ensure that the liquid and the pipette/tip combination are at approximately
the same temperature
−Choose the right tip volume for your pipette: the colour of the tip tray
should match the colour code on the pipette
−Use Optifit Tips in rack, refill, or bulk packaging, and choose the purity
level your application requires: free of DNase, RNase and endotoxin, and/or
sterilized
−If aerosol contamination needs to be avoided, choose Safe-Cone Filters,
which are attached to the tip-cone and should be changed daily, or
SafetySpace Filter Tips, which are discarded after each pipetting
−SafetySpace Filter Tips should also be chosen when you want to avoid
sample loss due to the sample reaching the filter. The extra space between
the sample and the filter ensures that even foaming or viscous liquids don’t
reach the filter in reverse pipetting or repetitive/multiple dispensing modes
−Pre-rinse the tip three to five times before pipetting (this is especially
important in the forward pipetting mode)
−Wipe the tip against the receiving vessel’s wall to catch the last droplet
after dispensing
−Change the tip after every pipetting