GE AKTA GO User manual

ÄKTA™go
Operating Instructions
Original instructions

Table of Contents
41 Introduction ..........................................................................................................
51.1 Important user information .............................................................................................................
61.2 About this manual ................................................................................................................................
71.3 Associated documentation ..............................................................................................................
102 Safety instructions ...............................................................................................
112.1 Safety precautions ...............................................................................................................................
142.2 Labels .........................................................................................................................................................
152.3 Emergency procedures ......................................................................................................................
173 System description ..............................................................................................
183.1 ÄKTA go .....................................................................................................................................................
243.2 Available modules ................................................................................................................................
273.3 UNICORN ...................................................................................................................................................
314 Installation ............................................................................................................
324.1 Site preparation .....................................................................................................................................
334.1.1 Delivery, storage, and unpacking ...........................................................................................
354.1.2 Space requirements ......................................................................................................................
374.1.3 Site environment ............................................................................................................................
394.1.4 Power requirements .....................................................................................................................
404.1.5 Computer requirements ..............................................................................................................
414.1.6 Required materials ........................................................................................................................
424.2 Hardware installation .........................................................................................................................
434.2.1 Connect the system ......................................................................................................................
464.2.2 Install waste tubing .......................................................................................................................
484.2.3 Prepare the pump rinsing system ...........................................................................................
514.2.4 Start the instrument ......................................................................................................................
524.3 Software installation ...........................................................................................................................
534.3.1 Download and Install UNICORN ..............................................................................................
544.3.2 Download the Instrument Configuration ............................................................................
554.3.3 Adapt UNICORN to your system ..............................................................................................
574.4 Start UNICORN and connect to system ......................................................................................
605 Prepare the system for a run .............................................................................
615.1 Prepare the flow paths .......................................................................................................................
645.2 Prime inlets and purge pump heads ...........................................................................................
705.3 Performance tests ................................................................................................................................
745.4 Connect a column ................................................................................................................................
775.5 Pressure alarms .....................................................................................................................................
785.6 Prepare for a run at low temperature .........................................................................................
796 Run a method ........................................................................................................
806.1 Create a method ...................................................................................................................................
826.2 Prepare sample for loading .............................................................................................................
2 ÄKTA go Operating Instructions 29360951 AC
Table of Contents

846.3 Start a method run ..............................................................................................................................
856.4 Monitor or interact with the run ....................................................................................................
866.5 Evaluate the run ....................................................................................................................................
876.6 Procedures after the run ...................................................................................................................
907 Maintenance .........................................................................................................
917.1 Clean the instrument externally ....................................................................................................
927.2 Perform system Cleaning-In-Place (CIP) ....................................................................................
967.3 Replace pump rinsing liquid ............................................................................................................
977.4 Replace the main fuses ......................................................................................................................
998 Troubleshooting ...................................................................................................
1019 Reference information ........................................................................................
1029.1 System specifications .........................................................................................................................
1039.2 Chemical resistance specifications ..............................................................................................
1069.3 Recycling information .........................................................................................................................
1089.4 Regulatory information ......................................................................................................................
1099.4.1 European Union and European Economic Area ...............................................................
1109.4.2 Eurasian Customs Union ............................................................................................................
1119.4.3 Regulatory statements ................................................................................................................
1139.4.4 Declaration of Hazardous Substances (DoHS) ..................................................................
1159.5 Health and Safety Declaration Forms .........................................................................................
ÄKTA go Operating Instructions 29360951 AC 3
Table of Contents

1 Introduction
About this chapter
This chapter contains important information that must be read before operating the
ÄKTA go system.
In this chapter
See pageSection
51.1 Important user information
61.2 About this manual
71.3 Associated documentation
4 ÄKTA go Operating Instructions 29360951 AC
1 Introduction

1.1 Important user information
Read this before operating the
product
All users must read the entire Operating Instructions before installing, operating or
maintaining the product.
Always keep the Operating Instructions at hand when operating the product.
Do not operate the product in any other way than described in the user documentation.
If you do, you may be exposed to hazards that can lead to personal injury and you may
cause damage to the equipment.
Intended use of the product
The ÄKTA go instrument is intended for the purification of bio-molecules, in particular
proteins, for research purposes. It is intended to be used by trained laboratory staff
members in research laboratories within academia and industry.
The ÄKTA go instrument must not be used in any clinical procedures, or for diagnostic
purposes.
The ÄKTA go is not intended for Reversed Phase Chromatography (RPC), since the EPDM
membranes in the K9 Inlet valve are not compatible with buffers normally used in RPC.
Prerequisites
In order to operate ÄKTA go in the way it is intended:
•The user must know how to use a computer with Microsoft®Windows®.
•The user must understand the concepts of liquid chromatography.
•The user must read and understand the Safety instructions chapter in the Operating
Instructions.
•The ÄKTA go instrument must be installed in accordance with the site requirements
and instructions in the Operating Instructions.
ÄKTA go Operating Instructions 29360951 AC 5
1 Introduction
1.1 Important user information

1.2 About this manual
Purpose of this manual
The Operating Instructions give the information needed to install, operate, and maintain
the product in a safe way. Translations of the original instructions are given in several
languages and are contained in the CD provided with this manual, or can be found online
at www.gelifesciences.com/aktago.
Scope of this manual
The Operating Instructions cover the ÄKTA go system.
Typographical conventions
Software items are identified in the text by bold italic text. A colon separates menu levels,
thus File:Open refers to the Open command in the File menu.
Hardware items are identified in the text by bold text (for example, Power).
Notes and tips
A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Note:
A tip contains useful information that can improve or optimize your procedures.
Tip:
6 ÄKTA go Operating Instructions 29360951 AC
1 Introduction
1.2 About this manual

1.3 Associated documentation
Introduction
This section describes the user documentation that is delivered with the product, and
how to find related documentation that can be downloaded from the GE Healthcare
website.
ÄKTA go user documentation
The user documentation listed in the table below is available in printed or in PDF format
at www.gelifesciences.com/aktago.
Main contentsDocumentation
Information needed to handle the delivery package
and unpack the ÄKTA go instrument.
ÄKTA go Unpacking
Instructions
Information needed to install, operate, and maintain
the ÄKTA go system in a safe way.
ÄKTA go Operating
Instructions
Translations of the original instructions are given in
several languages.
Essential information to be kept near the ÄKTA go
system.
ÄKTA go Cue Cards
Additional detailed information on the system, com-
ponent functions, and maintenance. Tips on how to
get the most out of the system when running it.
ÄKTA go User Manual
General specifications and list of materials in the flow
path.
ÄKTA go Product
Documentation
Instructions on how to prepare the installation site for
the ÄKTA go system.
ÄKTA go Site Preparation
Guide
ÄKTA go Operating Instructions 29360951 AC 7
1 Introduction
1.3 Associated documentation

Access from the web
Follow the steps below to access ÄKTA go user documentation that is available on the
web.
ActionStep
To access the product page for ÄKTA go, scan the code below using your
mobile device, or go to www.gelifesciences.com/aktago.
1
Click RELATED DOCUMENTS.2
Select the document you require.3
UNICORN™user documentation
The UNICORN user documentation is listed in the following table and is available from
the UNICORN software. Use the Help menu or press the F1 key on your keyboard. The
documents are available in the UNICORN Online Help and Documentation section.
Main contentsDocumentation
Detailed instructions on how to install UNICORN.UNICORN Quick Installation
Guide 1
•Overview and detailed description of network
setup and complete software installation.
•Administration of UNICORN and the UNICORN
database.
UNICORN Administration and
Technical Manual 2
•Overview and detailed descriptions of the
method creation features in UNICORN.
•Workflow descriptions for common operations.
UNICORN Method Manual 2
8 ÄKTA go Operating Instructions 29360951 AC
1 Introduction
1.3 Associated documentation

Main contentsDocumentation
•Overview and detailed description of the system
control features in UNICORN.
•Includes general operation, system settings and
instructions on how to perform a run.
UNICORN System Control
Manual 2
•Overview and detailed descriptions of the
Evaluation Classic3module in UNICORN.
•Description of the evaluation algorithms used in
UNICORN.
UNICORN Evaluation
Manual 2
•Video clips showing common workflows in the
Evaluation module.
•Overview of features of the Evaluation module.
Getting started with
Evaluation (accessed through
help in the UNICORN
Evaluation module)
Descriptions are displayed for the currently active
pane or dialog box.
UNICORN Help
1The UNICORN Quick Installation Guide can be downloaded from www.gelifesciences.com/aktago.
2The current UNICORN version is also added to the title of the manual.
3Evaluation Classic is an advanced evaluation module that requires an extra license to run.
ÄKTA go Operating Instructions 29360951 AC 9
1 Introduction
1.3 Associated documentation

2 Safety instructions
About this chapter
This chapter contains important information for your personal safety.
In this chapter
See pageSection
112.1 Safety precautions
142.2 Labels
152.3 Emergency procedures
10 ÄKTA go Operating Instructions 29360951 AC
2 Safety instructions

2.1 Safety precautions
Introduction
ÄKTA go is powered by mains voltage and handles materials that can be considered
hazardous. Before installing, operating or maintaining the system, you must be aware
of the hazards described in this manual.
Definitions
This user documentation contains safety notices (WARNING, CAUTION, and NOTICE)
concerning the safe use of the product. See definitions below.
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to pro-
ceed until all stated conditions are met and clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
ÄKTA go Operating Instructions 29360951 AC 11
2 Safety instructions
2.1 Safety precautions

General precautions
The following general precautions must be considered at all times. There are also context
related precautions, which are written in their respective chapters.
WARNING
Do not operate the product in any other way than described in the
ÄKTA go user documentation.
WARNING
Only properly trained personnel may operate and maintain the
product.
WARNING
Do not use any accessories not supplied or recommended by GE
Healthcare.
WARNING
Do not use ÄKTA go if it is not working properly, or if it has suffered
any damage, for example:
•damage to the power cord or its plug
•damage caused by dropping the equipment
•damage caused by splashing liquid onto it
WARNING
Access to power plug. Do not block access to the power outlet
and power plug. The power cord with plug must always be easy
to disconnect.
12 ÄKTA go Operating Instructions 29360951 AC
2 Safety instructions
2.1 Safety precautions

WARNING
In the event of a large spillage, disconnect the power cord from
the wall socket.
WARNING
Always use appropriate Personal Protective Equipment (PPE) during
operation and maintenance of this product.
WARNING
Hazardous substances and biological agents. When using haz-
ardous chemical and biological agents, take all suitable protective
measures, such as wearing protective clothing, glasses and gloves
resistant to the substances used. Follow local and/or national
regulations for safe operation and maintenance of this product.
WARNING
Spread of biological agents. The operator must take all necessary
actions to avoid spreading hazardous biological agents. The facility
must comply with the national code of practice for biosafety.
WARNING
High pressure. The product operates under high pressure. Wear
protective glasses and other required Personal Protective Equip-
ment (PPE) at all times.
WARNING
Explosive environment. The product is not approved for work in
a potentially explosive atmosphere. The product does not fulfill the
requirements of the ATEX Directive.
ÄKTA go Operating Instructions 29360951 AC 13
2 Safety instructions
2.1 Safety precautions

2.2 Labels
Description of information on the
system label
The following safety symbols and information may be present on the system label.
MeaningLabel
Warning! Read the user documentation before using the system.
Do not open any covers or replace parts unless specifically stated
in the user documentation.
Electrical rating: Voltage (VAC )Voltage
Electrical rating: Frequency (Hz)Frequency
Electrical rating: Maximum power consumption (VA)Max. Power
Degree of protection provided by the enclosureProtection
Class
Year (YYYY) and month (MM) of manufactureMfg. Date
14 ÄKTA go Operating Instructions 29360951 AC
2 Safety instructions
2.2 Labels

2.3 Emergency procedures
Introduction
This section describes how to shut down the ÄKTA go instrument in an emergency situ-
ation, and the procedure for restarting the system.
The section also describes the result in the event of power failure.
Emergency shutdown
To shut down the instrument in an emergency, disconnect the instrument power cord
from its power source. The power source can be an ordinary power outlet, or an UPS
(Uninterruptible Power Supply) unit.
Any ongoing activity will be terminated immediately. Run data up to the time of the in-
terruption will be saved.
NOTICE
Do not leave the instrument in an emergency stop condition. Flush
the flow path with water or buffer when the emergency has been
dealt with.
Power failure
The result of a power failure depends on whether the system is equipped with a Real-Time
Unit and whether the instrument or the computer is affected.
If power is lost to the ÄKTA go instrument, with or without a Real-Time Unit, the run is
interrupted immediately. Run data collected up to the time of the power failure is saved
in the UNICORN software.
If power is lost to the computer and the system is not equipped with a Real-Time Unit,
the run is interrupted immediately. Run data collected up to the time of the power failure
is saved in the UNICORN software.
If power is lost to the computer and the system is equipped with a Real-Time Unit, the
run continues to completion and the run data is stored in the Real-Time Unit and uploaded
to the computer once it is reconnected.
Connecting the instrument and computer to an uninterruptible power supply
(UPS) can help to prevent loss of data and material during a power failure.
Note:
ÄKTA go Operating Instructions 29360951 AC 15
2 Safety instructions
2.3 Emergency procedures

Restart after emergency
shutdown or power failure
Follow the steps below to restart the system after an emergency shutdown or power
failure.
ActionStep
Reconnect the power cord.1
Start the instrument by pressing the On/Off button on the instrument control
panel.
2
Start the computer and the UNICORN software.3
Re-establish connection between UNICORN and the instrument.4
If the run has been aborted, recover or discard remaining sample and flush
the flow path as appropriate.
5
16 ÄKTA go Operating Instructions 29360951 AC
2 Safety instructions
2.3 Emergency procedures

3 System description
About this chapter
This chapter gives an overview of the ÄKTA go instrument and the UNICORN software.
In this chapter
See pageSection
183.1 ÄKTA go
243.2 Available modules
273.3 UNICORN
ÄKTA go Operating Instructions 29360951 AC 17
3 System description

3.1 ÄKTA go
Introduction
This section gives an overview of the ÄKTA go instrument.
Illustration of the ÄKTA go
system
The illustration below shows the ÄKTA go system. The computer with the UNICORN
software is located on the right hand side of the instrument to make room for accessories
on the left hand side of the instrument.
Run
Exterior design
The liquid handling modules are placed on the front of the instrument. The instrument
is equipped with trays to collect spillage, and adjustable feet to level the instrument.
Buffer vessels can be placed on top of the instrument. Air ventilation, power cables, and
data cables are located at the rear of the instrument.
18 ÄKTA go Operating Instructions 29360951 AC
3 System description
3.1 ÄKTA go

Illustration of the ÄKTA go
instrument
The illustration below shows the ÄKTA go instrument after installation.
FunctionPart
Pump1
Pump rinsing solution tube2
Pressure monitor3
Mixer4
Injection valve5
UV monitor6
Top tray7
Holder rails8
Instrument control panel9
Conductivity monitor10
Flow restrictor11
Outlet valve12
Inlet valve13
Bottom tray14
Ready
8
9
10
11
12
13
14
2
1
6
3
4
5
7
Illustration of the instrument
control panel
The instrument control panel is located to the upper left on the front of the instrument.
It shows the current status of the system using LED light and status text. The pause and
continue buttons can be used to control an ongoing run.
Control panel buttons can be locked using the UNICORN software.
Note:
ÄKTA go Operating Instructions 29360951 AC 19
3 System description
3.1 ÄKTA go

FunctionPartIllustration
Display1
1
2
3
4
5
Status indicator2
Pause button3
Run/Continue button4
On/Off button5
Status indicators
The display and status indicators on the instrument control panel indicate the current
status of ÄKTA go.
The table below describes the different states that can be displayed.
DescriptionDisplayState
The instrument is turned off.Off
20 ÄKTA go Operating Instructions 29360951 AC
3 System description
3.1 ÄKTA go