Sartorius Vivaspin Turbo 15 RC User manual

Instructions for Use
Vivaspin®Turbo 15 RC
Centrifugal Ultrafiltration Devices for Research Use
2563320-000-02

Contents
Instructions for Use Vivaspin®Turbo 15 RC 3
Contents
1 About these Instructions.................................................5
1.1 Scope .................................................................5
1.2 Target Groups.........................................................5
1.3 Symbols Used.........................................................6
1.3.1 Warnings in Operation Descriptions...........................6
1.3.2 Other Symbols.................................................6
2 Safety Instructions .......................................................7
2.1 General Functions ....................................................7
2.2 Personnel Qualification ...............................................7
2.3 Significance of these Instructions.....................................8
2.4 Functionality of the Product ..........................................8
3 Product Description .....................................................9
3.1 Product Overview.....................................................9
3.2 Product Symbols.....................................................10
4 Process Preparation......................................................11
4.1 Scope of Delivery .....................................................11
4.2 Unpacking ............................................................11
5 Operation...............................................................12
5.1 Pre-Rinsing the Product .............................................12
5.2 Sanitizing the Product ...............................................12
5.3 Performing Filtration.................................................13
5.3.1 Applying the sample..........................................13
5.3.2 Inserting the Product into the Centrifuge ....................14
5.3.3 Performing Filtration .........................................14
5.3.4 Removing the Sample ........................................15
5.4 Desalting or Buffer Exchange ........................................15
6 Storage .................................................................16
6.1 Storing the Product ..................................................16
7 Disposal.................................................................17
7.1 Decontaminating the Product .......................................17
7.2 Disposing of the Product.............................................17

Contents
4 Instructions for Use Vivaspin®Turbo 15 RC
8 Technical Specifications ................................................18
8.1 Dimensions ..........................................................18
8.2 Materials .............................................................18
8.3 Ambient Conditions..................................................19
8.4 Operating Conditions................................................19
8.5 Equipment Required................................................20
8.5.1 Pipettes......................................................20
8.5.2 Centrifuges ..................................................20
8.6 Sanitizing Methods .................................................20
8.7 Chemical Compatibility ..............................................21
8.8 Typical Performance Characteristics.................................22
8.8.1 Typical Concentration Conditions ............................22
8.8.2 Examples of Times to Concentrate 30x at 20 °C and Solute
Recoveries....................................................23

About these Instructions
Instructions for Use Vivaspin®Turbo 15 RC 5
1 About these Instructions
1.1 Scope
These instructions are part of the product. These instructions apply to the
following versions of the product:
Vivaspin®Turbo 15 RC Quantity Prod. no.
5 kDa 12 | 48 VS15TR11 | VS15TR12
10 kDa 12 | 48 VS15TR01 | VS15TR02
30 kDa 12 | 48 VS15TR21 | VS15TR22
50 kDa 12 | 48 VS15TR31 | VS15TR32
100 kDa 12 | 48 VS15TR41 | VS15TR42
1.2 Target Groups
The instructions are addressed to the following target groups. The target
groups must possess the knowledge listed below.
Target Group Knowledge and Qualifications
Operator The operator is familiarwith the device and the
associated work processes. The operator understands
the hazards which may arise when working with the
device, and knows how to prevent them.

About these Instructions
6 Instructions for Use Vivaspin®Turbo 15 RC
1.3 Symbols Used
1.3.1 Warnings in Operation Descriptions
NOTICE
Denotes a hazard that may result in property damage if it is not avoided.
1.3.2 Other Symbols
t  Required action: Describes actions that must be carried out.
The actions in the sequence must be carried out in succession.
y Result: Describes the result of the actions carried out.

Safety Instructions
Instructions for Use Vivaspin®Turbo 15 RC 7
2 Safety Instructions
2.1 General Functions
The product is intended for the ultrafiltration and | or diafiltration of biological
and aqueous solutions. The sample solutions and volumes used must be suit-
able for the product.
The filtration process must be carried out in a centrifuge. Macromolecules
that are sufficiently larger than the nominal pore size of the membrane are re-
tained above the membrane and progressively concentrated. The vertical
membrane inhibits membrane fouling while the built-in dead stop impedes
concentration to dryness and loss of sample.
The product is supplied non-sterile. It is intended for single use and must be
disposed of after one use.
The product is intended exclusively for use in accordance with these instruc-
tions. Any further use beyond this is considered improper.
Operating Conditions for the Product
The product may only be used for research purposes. Do not use the product
for in vitro diagnostic procedures or similar diagnostic procedures.
The product may only be used with the equipment and under the operating
conditions described in the Technical Data section of these instructions.
2.2 Personnel Qualification
Persons without sufficient knowledge in the safe use of the device can injure
themselves and others.
If a specific qualification is required for an activity:The target group is indicated.
If no qualification is specified: The activity can be carried out by the target
group “Operator”.

Safety Instructions
8 Instructions for Use Vivaspin®Turbo 15 RC
2.3 Significance of these Instructions
Failure to follow the instructions might have serious consequences, e.g. dan-
ger to individuals.
t Read the instructions carefully and completely. The instructions for action
build on each other.
t Ensure that the information contained in these instructions is available to
all individuals working with the product.
2.4 Functionality of the Product
A damaged product or worn parts can lead to malfunctions or cause hazards
which are difficult to identify.
t Only operate the product when it is safe and in perfect working order.

Product Description
Instructions for Use Vivaspin®Turbo 15 RC 9
3 Product Description
3.1 Product Overview
4
1
2
5
3
Fig.1: Product overview (example)
Pos. Description
1 Concentrator cap
2 Concentrator
3Twin vertical membranes
4 Filtrate container
5 Angular dead stop pocket

Product Description
10 Instructions for Use Vivaspin®Turbo 15 RC
3.2 Product Symbols
Symbol Description
Catalogue number
Batch code
Use by
Non-sterile product
Only suitable for research purposes
Do not re-use
Consult instructions for use
Temperature limits

Process Preparation
Instructions for Use Vivaspin®Turbo 15 RC 11
4 Process Preparation
4.1 Scope of Delivery
Article Quantity
Product, packed in a cardboard box 12 or 48
Instructions for Use 1
4.2 Unpacking
Procedure
t NOTICE Risk of product malfunctions due to exceeding the usability!
Check the usability of the product (see specification on packaging).
Dispose of products forwhich the usability has been exceeded.
t Unpack the product.

Operation
12 Instructions for Use Vivaspin®Turbo 15 RC
5 Operation
5.1 Pre-Rinsing the Product
Membranes in the product may contain traces of glycerin. If this substance
can interfere with the analysis of the sample: The membranes may be rinsed
before filtration.
Procedure
t Remove the concentrator cap.
t Use a pipette to apply a filling volume of buffer solution or deionized water
into the concentrator.
t Replace the concentrator cap.
t Wash the buffer solution or deionized water through the membranes by
centrifugation.
t Empty the concentrator and filtrate container.
t If the pre-rinsed product is not used immediately: Cover the surface of the
membranes with buffer solution or water and store the product in the
refrigerator. The membranes must not dry out.
5.2 Sanitizing the Product
The product can be sanitized before use. The sanitizing method must be
suitable for the product (see Chapter 8.4, page 19).
Procedure
t Remove the concentrator cap.
t Sanitize the product using the desired sanitizing method.
t Empty the product.

Operation
Instructions for Use Vivaspin®Turbo 15 RC 13
5.3 Performing Filtration
5.3.1 Applying the sample
It is recommended that a pipette is used to apply the sample into the product.
The pipette must be compatible with the product (see Chapter “8.5.1 Pipettes”,
page 20).
Please ensure that the molecular weight cut-off (MWCO) of the product is
suitable for the size of the target molecule to be concentrated. In order to
ensure maximum recovery of the target molecule, Sartorius recommends
selecting a MWCO that is at least 50% below the size of the target molecule.
NOTICE
Risk of product malfunctions due to using unsuitable samples!
t Only pour suitable samples into the product (see Chapter “8.7 Chemical
Compatibility”, page 21).
NOTICE
Risk of product malfunctions or damage to the centrifuge due to exceeding
the maximum filling volume!
t Do not exceed the maximum filling volume (see Chapter “8.4 Operating
Conditions”, page 19).
Procedure
t Check whether the MWCO of the product is suitable for the application.
t Remove the concentrator cap.
t Apply the sample into the product using a pipette. Comply with the maxi-
mum filling volume.
t Replace the concentrator cap.

Operation
14 Instructions for Use Vivaspin®Turbo 15 RC
5.3.2 Inserting the Product into the Centrifuge
Procedure
t NOTICE Risk of product malfunctions or dam-
age to the centrifuge! Only use the product in
suitable centrifuges (see Chapter “8.5.2 Cen-
trifuges”, page 20).
t Insert the product into the centrifuge.
t If a centrifuge with fixed-angle rotor is used:
Place the product into the centrifuge as follows:
−The printed volume graduations on the
concentrator (1) are facing sideways.
−The dead stop pocket of the concentrator
(2) is pointing towards the outside of the
fixed-angle rotor.
5.3.3 Performing Filtration
Procedure
t NOTICE Risk of product malfunctions or dam-
age to the centrifuge. Comply with the ap-
proved centrifugation limit values (see Chap-
ter “8.4 Operating Conditions”, page 19).
t Centrifuge the product in the centrifuge until
the desired concentration level is achieved.
2
1

Operation
Instructions for Use Vivaspin®Turbo 15 RC 15
5.3.4 Removing the Sample
Procedure
t If the filtration or concentration is complete: Remove the product from the
centrifuge.
t Remove the concentrator cap.
t Recover the sample from dead stop pocket of the concentrator using a
pipette.
t If the membrane was pre-rinsed before filtration: Decant the filtrate and
concentrate.
5.4 Desalting or Buffer Exchange
Procedure
t Concentrate the sample to the desired level.
t Remove the concentrator cap.
t Discard the filtrate.
t Refill the concentratorwith an appropriate exchange buffer.
t Concentrate the sample again.
t Repeat the process until the original buffer and | or contaminating micro-
solute has been sufficiently removed.
t If the desalting or buffer exchange is complete: Recover the sample.

Storage
16 Instructions for Use Vivaspin®Turbo 15 RC
6 Storage
6.1 Storing the Product
If the product has been unpacked and the membranes have been pre-rinsed:
The membranes must be protected against drying out. For this purpose, the
membranes must be stored in a moist and cool condition.
NOTICE
Risk of damage to the product due to improper storage!
t Complywith the storage specifications.
Procedure
t If the product is packaged: Store the product in the packaging.
t If the product has been unpacked and the membranes have been pre-
rinsed:
t Remove the concentrator cap.
t Cover the membranes with buffer solution orwater.
t Replace the concentrator cap.
t Store the product according to the ambient conditions (see Chapter 8.3,
page 19).

Disposal
Instructions for Use Vivaspin®Turbo 15 RC 17
7 Disposal
7.1 Decontaminating the Product
If the product has come into contact with hazardous substances: Steps must
be taken to ensure proper decontamination and declaration. The operator of
the product is responsible for adhering to local government regulations on
the proper decontamination and declaration for transport and disposal.
Procedure
t If the product has come into contact with hazardous substances:
Decontaminate the product.
7.2 Disposing of the Product
The product must be disposed of properly. The packaging is made of environ-
mentally friendly materials that can be used as secondary raw materials.
Requirements
The product must be decontaminated.
Procedure
t Dispose of the product in accordance with local government regulations.
t Dispose of the packaging in accordance with local government regulations.

Technical Specifications
18 Instructions for Use Vivaspin®Turbo 15 RC
8 Technical Specifications
8.1 Dimensions
Unit Value
Length x Diameter mm 118 x 30
Active membrane surface cm28.6
Weight g 25
8.2 Materials
Materials
Concentrator Styrene butadiene copolymer (SBC)
Concentrator cap | Filtrate container Polypropylene
Membranes Regenerated cellulose

Technical Specifications
Instructions for Use Vivaspin®Turbo 15 RC 19
8.3 Ambient Conditions
Unit Value
Storage temperature
When packed °C +4 – +40
When unpacked, with membrane kept moist °C +2 – +8
8.4 Operating Conditions
Centrifuge
with swing
bucket rotor
Centrifuge with
fixed-angle
rotor (25°)
Unit Value Value
For use with centrifuge
Filling volume, minimum mL 4 4
Filling volume, maximum mL 15 11
Membrane hold-up
volume, minimum
µL 28 28
Dead stop volume1µL 120 140
Relative Centrifugal Force,
maximum
5 – 50 kDa g4,000 6,000
100 kDa g3,000 6,000
1 The dead stopvolume mayvary depending on the type and concentration of the sample,
operating temperature and | or centrifuge rotor

Technical Specifications
20 Instructions for Use Vivaspin®Turbo 15 RC
8.5 Equipment Required
8.5.1 Pipettes
Pasteur pipette, variable volume or fixed volume pipette for sample appli-
cation and concentrate or filtrate retrieval
8.5.2 Centrifuges
Unit Value
Swing bucket rotor or fixed-angle rotor
Minimum rotor angle for fixed-angle rotor 25°
Rotor suitable for centrifuge tubes with the
following properties
Conical base
Volume mL 50
Diameter mm 30
8.6 Sanitizing Methods
Rinsing with 70% ethanol solution or with sanitizing gas mixture,
e.g.ethylene oxide
Not suitable for autoclaving