Sartorius Centrisart G-16 User manual

Translation of the original operating manual
sb
09/2015
Rev. 1.3 of 02/03/2017
G-16;Version; ; ;
Version
WZG6001-e150401
Operating Manual
CentrisartG-16
Laboratory centrifuge from serial no.
157603
98648-020-94

© Copyright by
Sartorius Lab Instruments GmbH & Co. KG
Weender Landstraße 94-108
37075 Göttingen
Germany
Phone: [phone]
Fax: [phone]
www.sartorius.com

Table of contents
Version 09/2015, Rev. 1.3 of 02/03/2017
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Translation of the original operating manual, WZG6001-e150401
1General information...........................................................................................................................................9
1.1 Importance of the operating manual......................................................................................................................9
1.2 Intended use....................................................................................................................................................................9
1.3 Warranty and liability..................................................................................................................................................9
1.4 Copyright.......................................................................................................................................................................10
1.5 Standards and regulations........................................................................................................................................10
1.6 Scope of supply............................................................................................................................................................10
2Layout and mode of operation.......................................................................................................................11
2.1 Layout of the centrifuge...........................................................................................................................................11
2.1.1 Functional and operating elements................................................................................................................11
2.1.2 Name plate.............................................................................................................................................................12
2.2 Mode of operation......................................................................................................................................................13
2.2.1 Centrifugation principle.....................................................................................................................................13
2.2.2 Area of application...............................................................................................................................................13
2.2.2.1 Speed, radius, and relative centrifugal force.....................................................................................14
2.2.2.2 Density...........................................................................................................................................................14
3Safety...................................................................................................................................................................15
3.1 Marking of the unit....................................................................................................................................................15
3.2 Explanation of the symbols and notes..................................................................................................................16
3.3 Responsibility of the operator.................................................................................................................................17
3.4 Operating personnel...................................................................................................................................................17
3.5 Informal safety instructions.....................................................................................................................................17
3.6 Safety instructions......................................................................................................................................................18
3.6.1 Electrical safety.....................................................................................................................................................18
3.6.2 Mechanical safety.................................................................................................................................................18
3.6.3 Fire prevention......................................................................................................................................................19
3.6.4 Chemical and biological safety.........................................................................................................................19
3.6.5 Safety instructions for centrifugation...........................................................................................................20
3.6.6 Resistance of plastics...........................................................................................................................................20
3.6.7 Service life of rotors and accessories..............................................................................................................20
3.7 Safety devices...............................................................................................................................................................21
3.7.1 Lid lock device.......................................................................................................................................................21
3.7.2 Standstill monitoring system............................................................................................................................21
3.7.3 System check..........................................................................................................................................................21
3.7.4 Earth conductor check........................................................................................................................................21
3.7.5 Imbalance monitoring system...........................................................................................................................21
3.7.6 Rotor monitoring system....................................................................................................................................21
3.8 Measures in the event of hazards and accidents...............................................................................................22
3.9 Remaining hazards......................................................................................................................................................22

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4Storage and transport......................................................................................................................................23
4.1 Dimensions and weight..............................................................................................................................................23
4.2 Storage conditions......................................................................................................................................................23
4.3 Notes on transport......................................................................................................................................................23
4.4 Packaging.......................................................................................................................................................................24
4.5 Transport safety device..............................................................................................................................................24
5Set-up and connection ....................................................................................................................................25
5.1 Installation site.............................................................................................................................................................25
5.2 Power supply.................................................................................................................................................................25
5.2.1 Type of connection...............................................................................................................................................25
5.2.2 Customer-provided fuses...................................................................................................................................25
6Using the centrifuge.........................................................................................................................................26
6.1 Initial start-up..............................................................................................................................................................26
6.2 Switching the centrifuge on....................................................................................................................................26
6.2.1 Opening and closing the lid...............................................................................................................................26
6.2.2 Installation of rotors and accessories.............................................................................................................27
6.2.2.1 Installation of the rotor...........................................................................................................................27
6.2.2.2 Installation of angle rotors with a hermetically sealed lid............................................................27
6.2.2.3 Installation of accessories........................................................................................................................28
6.2.2.4 Adapters........................................................................................................................................................29
6.2.2.5 Tubes..............................................................................................................................................................29
6.3 Control system "Spincontrol L"................................................................................................................................30
6.3.1 User interface........................................................................................................................................................30
6.3.2 Manual mode.........................................................................................................................................................31
6.3.2.1 Starting a centrifugation run.................................................................................................................31
6.3.2.2 Interrupting a centrifugation run.........................................................................................................31
6.3.2.3 Interrupting a deceleration process......................................................................................................31
6.3.2.4 Selection, display, and modification of data.....................................................................................31
6.3.2.5 Speed / relative centrifugal force (RCF)..............................................................................................32
6.3.2.6 Runtime.........................................................................................................................................................32
6.3.2.7 Rotor selection............................................................................................................................................34
6.3.2.8 Acceleration and deceleration curves..................................................................................................35
6.3.2.9 Automatic lid opening function ("AutoOpen").................................................................................35
6.3.2.10 Sound signal ("Buzzer")............................................................................................................................36
6.3.2.11 Program lock ("ProgLock").......................................................................................................................36
6.3.2.12 Cycle display ("Cycles").............................................................................................................................37
6.3.2.13 Input lock.....................................................................................................................................................38
6.3.3 Program mode.......................................................................................................................................................39
6.3.3.1 Saving a program.......................................................................................................................................39
6.3.3.2 Loading a program.....................................................................................................................................40
6.4 Switching the centrifuge off....................................................................................................................................40

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7Malfunctions and error correction................................................................................................................41
7.1 General malfunctions.................................................................................................................................................41
7.1.1 Emergency lid release..........................................................................................................................................42
7.2 Table of error codes....................................................................................................................................................43
7.3 Service contact.............................................................................................................................................................44
8Maintenance and service.................................................................................................................................45
8.1 Maintenance.................................................................................................................................................................45
8.1.1 Centrifuge...............................................................................................................................................................45
8.1.2 Accessories..............................................................................................................................................................46
8.1.2.1 Plastic accessories......................................................................................................................................47
8.1.3 Rotors, buckets and carriers..............................................................................................................................47
8.1.4 Load bearing bolts................................................................................................................................................47
8.1.5 Glass breakage.......................................................................................................................................................48
8.2 Sterilisation and disinfection of the rotor chamber and accessories...........................................................48
8.2.1 Autoclaving............................................................................................................................................................49
8.3 Service.............................................................................................................................................................................50
8.4 Return of defective units..........................................................................................................................................51
9Disposal................................................................................................................................................................52
10 Technical data....................................................................................................................................................53
10.1 Ambient conditions.....................................................................................................................................................54
11 Appendix..............................................................................................................................................................55
11.1 Range of accessories...................................................................................................................................................55
11.1.1 Maximum speed for tubes.................................................................................................................................56
11.1.2 Rotor radii...............................................................................................................................................................56
11.2 Speed-gravitational-field-diagram........................................................................................................................57
11.3 Acceleration and deceleration curves....................................................................................................................58
11.4 Resistance data.............................................................................................................................................................61
11.5 EC declaration of conformity...................................................................................................................................65
12 Index.....................................................................................................................................................................67

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1 General information
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1General information
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1.1 Importance of the operating manual
A fundamental requirement for the safe and trouble-free operation of the
centrifuge is to be familiar with the fundamental safety instructions and all
possible hazards.
The operating manual includes important information concerning the safe
operation of the centrifuge.
This operating manual and, in particular, the notes on safety and hazards must be
observed by all persons operating the centrifuge.
In addition, the local rules and regulations for the prevention of accidents must be
complied with.
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1.2 Intended use
Centrifuges are power-driven machines that separate liquids from solid matter,
liquid mixtures, or solid mixtures by centrifugal force. They are solely intended for
this purpose. Any other use beyond this area of application is regarded as improper
use. Sartorius Lab Instruments GmbH & Co. KG cannot be held liable for any
damage resulting from such improper use.
The intended use also includes
observation of all the notes and instructions included in the operating manual
and
compliance with the care, cleaning, and maintenance instructions.
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1.3 Warranty and liability
The warranty and liability are subject to our "General Terms and Conditions of
Business Relating to Goods and Services of Sartorius" that were distributed to the
operator upon the conclusion of the contract.
Warranty and liability claims are excluded if they are due to:
improper use.
non-compliance with the safety instructions and hazard warnings in the
operating manual.
improper installation, start-up, operation, or maintenance of the centrifuge.
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1.4 Copyright The information and figures contained in these instructions correspond to the
version date specified below.
Sartorius reserves the right to make changes to the technology, features,
specifications and design of the equipment without notice.
Copyright notice:
This operating manual, including all of its components, is protected by copyright.
Any use beyond the limits of the copyright law is not permitted without our
approval.
This applies in particular to reprinting, translation and edition irrespective of the
type of media used.
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1.5 Standards and regulations
EC declaration of conformity
(see chapter 11.5 - "EC declaration of conformity")
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1.6 Scope of supply
The centrifuge comprises:
1 connection cable
depending on the voltage variant
1 box spanner, size 10
(rotor fastening)
Part no. 69CE0009
1 tube of grease for load bearing
bolts
Part no. 69CE0006
Documentation:
Operating manual incl. EC declaration of conformity
(see chapter 11.5 - "EC declaration of conformity")
Accessories
According to your order, our order confirmation, and your delivery note.
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2 Layout and mode of operation
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2Layout and mode of operation
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2.1 Layout of the centrifuge
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2.1.1 Functional and operating elements
1Lid
2User interface (see
chapter 6.3.1 - "User
interface")
3Mains switch
Fig. 1: Total view of the centrifuge
4 Name plate
(see chapter 2.1.2 -
"Name plate")
5 Mains power input
6 Equipotential bonding
screw
Fig. 2: Rear view of the centrifuge
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2.1.2 Name plate
1Manufacturer and
registered office
2Type
3Serial number
4Max. density
5Nominal voltage
6Input fuse
7Symbol for special
disposal (see chapter 9 -
"Disposal")
8CE-mark in compliance
with the directive
2006/42/EG
9Part number
10Year of manufacture
11Max. speed
12Max. kinetic energy
13Power consumption
Fig. 3: Example of a name plate
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2.2 Mode of operation
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2.2.1 Centrifugation principle
Centrifugation is a process for the separation of heterogeneous mixtures of
substances (suspensions, emulsions, or gas mixtures) into their components. The
mixture of substances, which rotates on a circular path, is subject to centripetal
acceleration that is several times greater than the gravitational acceleration.
Centrifuges use the mass inertia inside the rotor chamber for separating the
substances. Due to their higher inertia, particles or media with a higher density
travel outwards. In doing so, they displace the components with a lower density,
which in turn travel towards the centre.
The centripetal acceleration of an object inside a centrifuge, as the effect of
centripetal force, depends on the distance between the object and the axis of
rotation as well as on the angular velocity. It increases linearly as a function of the
distance with regard to the axis of rotation and quadratically as a function of the
angular velocity. The bigger the radius in the rotor chamber is and the higher the
speed is, the higher the centripetal acceleration is. However, the forces acting on
the rotor also increase.
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Das
2.2.2 Area of application
Depending on the area of application of the centrifuge and also on the particle
size, solids content, and volume throughput of the mixture of substances that is to
be centrifuged, there are different types of centrifuges.
The areas of application go from household use as a salad spinner or honey
separator up to specialised technical applications in the clinical, biological, or
biochemical context:
For numerous clinical examinations, cellular material must be separated from
the liquid to be analysed. The normal separation process can be sped up
considerably by using laboratory centrifuges.
In the metal-working industry, centrifuges are used for separating oil from
cream and low-fat milk.
Particularly big centrifuges are used in the sugar industry for separating the
syrup from the crystalline sugar.
Ultracentrifuges are predominantly used in biology and biochemistry in order
to isolate particles, e.g. viruses. They are specifically designed for high speeds up
to 500,000 rpm. The rotor moves in a vacuum in order to avoid air friction.
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2.2.2.1 Speed, radius, and relative centrifugal force
The acceleration g, which the samples are subject to, can be increased by increasing
the radius in the rotor chamber and by increasing the speed. These three
parameters are interdependent and linked with each other via the following
formula:
Relative centrifugal force RCF = 11.18 x 10-6 x r x n2
r = radius in cm
n = speed in rpm
RCF without any dimension
If two values are entered, the third value is determined by way of the stated
formula. If, afterwards, the speed or the radius is changed, the resulting relative
centrifugal force will be recalculated automatically by the control unit. If the RCF is
changed, the speed will be adapted while the specified radius is maintained.
The speed-gravitational-field-diagram provides an overview of the relationship
between speed, radius, and RCF (see chapter 11.2 - "Speed-gravitational-field-
diagram").
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2.2.2.2 Density The laboratory centrifuge is suitable for the separation of constituents of different
densities in mixtures with a maximum density of 1.2 g/cm3. All information
concerning the speed of rotors and accessories refers to liquids with a density
corresponding to this specification. If the density is above this value, the maximum
permissible speed of the centrifuge must be reduced based on the following
formula:
n = nmax x
)/2,1( Rho
Rho = density in g/cm3
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3Safety
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3.1 Marking of the unit
The following symbols are used for all types of centrifuges manufactured by Sartorius:
Dangerous voltage
I
On (Power)
Hot surface
0
Off (Power)
Caution! Risk of bruising
Name plate (see chapter 2.1.2
- "Name plate")
Protective earth (ground)
CE mark in compliance with
the directive 2006/42/EC
Earth (ground)
Do not dispose as part of
domestic waste
Unplug the mains plug
GS mark (tested safety; only
for Germany)
Arrow indicating the direction
of rotation
NRTL mark (only for the USA
and Canada)
Label concerning the FCC rules
(only for Canada)
RCM mark
(only for Australia)
China RoHS 2 mark
(only for China)

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NOTE
Safety indications on the centrifuge must be kept readable at all times. If
necessary, they must be replaced.
NOTE
Not all of the symbols/labels are used for this centrifuge type.
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3.2 Explanation of the symbols and notes
In this operating manual, the following names and symbols to indicate hazards are
used:
DANGER
This symbol stands for a direct hazard to the life and health of persons.
Non-observance of these symbols causes serious health problems up to life-
endangering injuries.
DANGER
This symbol stands for a direct hazard to the life and health of persons due to
electrical voltage.
Non-observance of these symbols causes serious health problems up to life-
endangering injuries.
WARNING
This symbol stands for a potential hazard to the life and health of persons.
Non-observance of these symbols can cause serious health problems up to life-
endangering injuries.
CAUTION
This symbol indicates a potentially hazardous situation
Non-observance of these notes can cause minor injuries or damage to property.
NOTE
This symbol indicates important information.
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3.3 Responsibility of the operator
The operator is responsible for authorising only qualified personnel to work on the
centrifuge (see chapter 3.4 - "Operating personnel").
The areas of responsibility of the personnel concerning the operation, maintenance,
and care of the unit must be clearly defined.
The safety-conscious work of the personnel in compliance with the operating
manual and the relevant EC and national health and safety regulations as well as
with the accident prevention regulations must be checked at regular intervals (e.g.
every month).
Under the international rules for health and safety at work, the operator is obliged
to:
take measures in order to prevent all danger to life or health during work.
ensure that centrifuges are operated properly and entirely as intended (see
chapter 1.2 - "Intended use").
take protective measures against fire and explosion when working with
hazardous substances.
take measures for the safe opening of centrifuges.
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3.4 Operating personnel
Persons operating the unit must
be familiar with the fundamental regulations concerning workplace safety and
accident prevention
have read and understood this operating manual (and in particular the safety
sections and warning notes) and confirmed this with their signature.
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
3.5 Informal safety instructions
This operating manual is a part of the product.
The operating manual must be kept at the location of use of the centrifuge.
Ensure that it is accessible at all times.
The operating manual must be handed over to any subsequent owner or
operator of the centrifuge.
Any changes made must be added to the operating manual.
In addition to the operating manual, the general and local rules and regulations
concerning the prevention of accidents and the protection of the environment
must also be supplied.
Safety and danger indications on the centrifuge must be kept readable at all
times. If necessary, they must be replaced.
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
3.6 Safety instructions
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
3.6.1 Electrical safety
To reduce the risk of electrical shock, the centrifuge uses a three-wire electrical
cord and plug to connect the equipment to earth-ground. To preserve this safety
feature:
DANGER
Ensure that the wall socket is properly wired and grounded.
Check that the mains voltage agrees with the nominal voltage listed on the
name plate.
Do not place vessels containing liquid on the centrifuge lid or within the safety
distance of 30 cm around the centrifuge. Spilled liquids may get into the
centrifuge and damage electrical or mechanical components.
Work on the power supply system must only be performed by certified
electricians.
Inspect the electrical equipment of the unit regularly. Defects such as loose or
burnt cables must be eliminated immediately.
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3.6.2 Mechanical safety
In order to ensure the safe operation of the centrifuge, observe the following:
WARNING
Do not open the lid when the rotor is in motion!
Do not reach into the rotor chamber when the rotor is in motion!
Do not use the centrifuge if it was installed incorrectly.
Do not use the centrifuge without panels.
Do not use the centrifuge if the rotors and inserts show signs of corrosion or
other defects.
Only use the centrifuge with rotors and accessories that have been approved
by the manufacturer. In case of doubt, contact the manufacturer (see chapter
7.3 - "Service contact").
Do not hold your fingers between the lid and the housing when closing the lid.
Risk of crushing!
Defective lid relieving devices could cause the centrifuge lid to fall (contact
the service department, if necessary). Risk of crushing!
Do not hit or move the centrifuge during its operation.
Do not lean against or rest on the centrifuge during its operation.
Do not spin any substances that could damage the material of the rotors and
buckets of the centrifuge in any way. Highly corrosive substances, for example,
damage the material and affect the mechanical strength of the rotors and
buckets.
Stop the centrifuge immediately in the event of a malfunction. Eliminate the
malfunction (see chapter 7 - "Malfunctions and error correction") or inform
the service department of the manufacturer (see chapter 7.3 - "Service
contact").
Ensure that all repairs are performed only by authorised and specialised
personnel.

3 Safety
Version 09/2015, Rev. 1.3 of 02/03/2017
19
Translation of the original operating manual, WZG6001-e150401
WARNING
Prior to any start-up, check the centrifuge, rotor, and accessories for signs of
damage that can be discerned from the outside. Special attention must be paid
to all of the rubber parts (e.g. motor cover, lid seal, and adapters) in terms of
visible structural changes. Defective parts must be replaced immediately.
Open the centrifuge when it is not in use so that moisture can evaporate.
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3.6.3 Fire prevention
DANGER
Do not spin explosive or inflammable substances.
Do not use the centrifuge within hazardous locations.
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3.6.4 Chemical and biological safety
If pathogenic, toxic, or radioactive samples are intended to be used in the
centrifuge, it is in the responsibility of the user to ensure that all necessary safety
regulations, guidelines, precautions, and practices are adhered to accordingly.
DANGER
Infectious, toxic, pathogenic, and radioactive substances may only be used in
special, certified containment systems with a bio-seal in order to prevent the
material from being released.
Take suitable precautions for your own safety if there is a risk of toxic,
radioactive, or pathogenic contamination
Materials that chemically react with each other with a high level of energy are
prohibited.
WARNING
Keep informed about local measures to avoid harmful emissions (depending on
the substances to be centrifuged).
Protective clothing is not required for the operation of the centrifuge.
The materials to be centrifuged may, however, require special safety measures
(e.g. centrifugation of infectious, toxic, radioactive, or pathogenic substances).
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3 Safety
20
Version 09/2015, Rev. 1.3 of 02/03/2017
Translation of the original operating manual, WZG6001-e150401
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3.6.5 Safety instructions for centrifugation
For safe operation, observe the following before starting the centrifuge:
WARNING
Ensure that the centrifuge was set up properly (see chapter 5 - "Set-up and
connection").
Maintain a safety distance of at least 30 cm (12 inches) around the centrifuge.
Do not store any dangerous goods in the centrifuge area.
Do not stay in the safety area longer than what is absolutely necessary for the
operation of the centrifuge.
Only use the centrifuge with rotors and accessories that have been approved
by the manufacturer. We explicitly warn against the use of equipment of poor
quality. Breaking glass or bursting vessels can cause dangerous imbalances at
high speeds
Ensure that rotor and buckets are correctly fitted (see chapter 6.2.2.1 -
"Installation of the rotor").
Observe the instructions on the installation of accessories (see chapter 6.2.2.3 -
"Installation of accessories").
WARNING
The rotor must be loaded axial symmetrically at equal weights.
If liquids with a density > 1.2 g/cm3are used, reduce the speed (see chapter
2.2.2.2 - "Density").
Do not use the centrifuge if the rotor is loaded asymmetrically.
Do not use the centrifuge with tubes that are excessively long.
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3.6.6 Resistance of plastics
Chemical influences have a strong effect on the polymeric chains of plastics, and,
therefore, on their physical properties. Plastic parts can be damaged if solvents,
acids, or alkaline solutions are used.
NOTE
Refer to the resistance data (see chapter 11.4 - "Resistance data")!
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3.6.7 Service life of rotors and accessories
The rotors and accessories have a limited service life.
WARNING
Perform regular checks (at least once per month) for safety reasons!
Pay special attention to changes, such as corrosion, cracks, material abrasion,
etc.
After 10 years, they must be inspected by the manufacturer.
After 50,000 cycles, the rotor must be scrapped for reasons of safety.
If other data concerning the service life are engraved on the rotor or bucket,
these data shall apply accordingly. For example, a bucket with the engraving
. date 02/20 ruary 2020 at the latest.
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