Sartorius eLINE User manual

eLINE®Electronic Pipette
User Manual

Contents
1. Intended Use ..................................................................................................................................2
2. eLINE® Electronic Pipettes........................................................................................................2
2.1. eLINE® single-, 8- and 12-channel pipettes ..............................................3
2.2. Sartorius pipette tips..........................................................................................3
3. Unpacking.......................................................................................................................................4
4. Pipette Description......................................................................................................................5
4.1. Display and Controls...........................................................................................5
4.2. Reset Button.......................................................................................................... 6
4.3. Start Button...........................................................................................................6
4.4. Tip Sealing and Electronic Tip Ejection........................................................ 8
4.5. Safe-Cone Filters................................................................................................ 8
5. Charging of the Pipette .............................................................................................................9
5.1. Charging with the Sartorius Charging Stand and Carousel.................9
5.2. Charging Directly...............................................................................................10
5.3. Electrical Specifications..................................................................................11
6. Programming Principle.............................................................................................................11
6.1. Programming Map.............................................................................................13
6.2. Speed Adjustment .............................................................................................14
7. Pipette modes..............................................................................................................................14
7.1. Super Pipetting (SP), Pipetting mode (P) and pipetting with
mixing (P+MIXING)............................................................................................15
7.2. Reverse pipetting (rP).......................................................................................16
7.3. Pipetting with cycle counter (P COUNT)...................................................17
7.4. Manual Pipetting (P MANUAL).....................................................................18
8. Multi-modes.................................................................................................................................19
8.1. Multi-dispensing (d)..........................................................................................20
8.2. Diluting (dd) and Diluting With Mixing (dd+MIXING)..........................21
8.3. Sequenced Dispensing (Sd) ............................................................................23
8.4. Multi-aspirating (SA)........................................................................................24
8.5. Automatic Dispensing (Ad).............................................................................25
9. Prog Mode.....................................................................................................................................26
9.1. Store operating mode to storage locations .............................................27
9.2. Recall stored mode from the storage location.......................................27
10. Pipetting Recommendations..................................................................................................28
10.1. Dispensing With Blow-out .............................................................................28
10.2. Dispensing Without Blow-out.......................................................................28
10.3. Other Recommendations ................................................................................28
11. Maintenance ................................................................................................................................29
11.1.1 Cleaning the Outer Surface of the Pipette (daily).................................29
11.1.2. Cleaning the Lower Part of a Single Channel Pipette
(every three months) .......................................................................................30
11.2.1 Cleaning and Autoclaving the Lower Part................................................30
11.2.2.Sterilizing..............................................................................................................31
11.2.3.Autoclaving..........................................................................................................32
11.2.4.UV Sterilization...................................................................................................32
11.3. Battery replacement.........................................................................................32
11.4. Storage...................................................................................................................33
12. Performance Testing.................................................................................................................33
12.1. Recalibration........................................................................................................35
13. Troubleshooting..........................................................................................................................37
14. Warranty Information..............................................................................................................39
15. Recycling Instruction (WEEE) ...............................................................................................40
16. Performance Specifications...................................................................................................40

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1. Intended Use
Sartorius eLINE® Electronic Pipettes are intended,
designed and manufactured for dispensing liquids in a
variety of applications, and are to be used in combination
with Sartorius Optifit Tips or SafetySpace Filter Tips. The
Sartorius eLINE® Electronic Pipette and tip combination,
fall within the scope of in-vitro diagnostics, and
can be used as a diagnostic medical device in related
applications. Thereby, Sartorius electronic pipettes and
Sartorius tips fulfil the relevant demands of the Directive
98/97/EC of the European Parliament.
The electronic pipette product range covers a volume
range of 0,1 µl to 5 ml. It is recommended that Sartorius
Optifit Tips or SafetySpace Filter Tips are used with
Sartorius pipettes to ensure optimum compatibility and
performance.
2. eLINE® Electronic
Pipettes
The eLINE® electronic pipettes offer you the specialised
technology and innovations in the field of the hand held
liquid handling devices. The attractive and ergonomic
design of the eLINE® pipettes together with electronic
tip ejection operation take the effort out of pipetting
and reduce the risk of repetitive strain injuries (RSI) that
are common in mechanical pipetting.
The microprocessor controlled system and the superior
construction of the eLINE® pipettes enables maximum
liquid handling performance with high levels of accuracy
and precision. The microprocessor controlled piston
movements reduces the possibility of human error and
thus also contamination of the pipette interior. The tip
cone design of the eLINE® pipettes offer the possibility
to use replaceable Safe-Cone Filters to help prevent
contamination and damage of the pipette.
The eLINE® electronic pipettes offers a comprehensive
range of liquid handling protocols, typically required by
laboratories currently. The simple keyboard and easy-to-
read display together with user-friendly operation and
programming allows these tasks to be performed quickly
and effortlessly. The eLINE® pipettes operate on the air
displacement principle and use disposable tips.

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2.1. eLINE® Single, 8 and 12 Channel Pipettes
Order
Code
Incl.
Charger
Order
Code
Excl.
Charger*
Channels Volume
Range
(µl)
Incre-
ment
(µl)
Optifit
Tips***
(µl)
Safety-
Space
Filter
Tips*** (µl)
Safe-Cone Filters
Standard Plus
730011 730010 10,1-5 0,05 10 10 – –
730021 730020 10,2-10 0,05 10 10 – –
730041 730040 15-120 0,50 200
350
120 721008 721018
730061 730060 110-300 1,00 350 300 721007 721017
730081 730080 150-1000 5,00 1000 1000 721006 721016
730101** 730100**1100-5000 10,0 5000 – 721006 721016
730321 730320 80,2-10 0,05 10 10 – –
730341 730340 85-120 0,50 200
350
120 721008 721018
730361 730360 810-300 1,00 350 300 721007 721017
730391 730390 850-1200 5,00 1200 1200 721006 721016
730421 730420 12 0,2-10 0,05 10 10 – –
730441 730440 12 5-120 0,50 200
350
300 721008 721018
730461 730460 12 10-300 1,00 350 300 721007 721017
730491 730490 12 50-1200 5,00 1200 1200 721006 721016
Pipettes are supplied with a universal charger (EU, UK, US|JPN, AUS, KOR and CHN plugs)
* Pipettes excluding a charger were discontinued as of 1st January, 2016.
** NOTE: Minimum volume in P-mode is 500 µl. 100 µl is possible in multi-dispensing (d) mode.
*** NOTE: Low Retention Tips are available in volumes up to
1200 µl.
2.2. Sartorius Pipette Tips
Sartorius pipette tips are recommended for use with
eLINE® pipettes. The use of fully compatible tips will
ensure the maximal pipetting accuracy and precision,
and thereby guarantee the performance specifications
given for the pipette. Sartorius tips are made of pure
virgin polypropylene and manufactured in protected
clean room conditions.

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Sartorius offers a full range of standard Optifit, and
SafetySpace Filter and Low Retention tips. Sartorius
non-filter tips are available in trays, bulk packages
and space-saving refill systems. Sartorius non-filter
tips and trays are autoclavable at 121°C (252F), 20
min, 1 bar overpressure (15 p.s.i). All Single Tray Racks
and clean Refill Packs are certified RNase, DNase and
endotoxin-free.
For more information, please go to
www.sartorius.com or contact your local Sartorius
support.
3. Unpacking
The eLINE® electronic pipettes may be delivered with
or without an AC-adaptor, depending from the type of
order. However, all eLINE® pipette packages contains:
- Pipette
- Tip
- Safe-Cone Filters and Filter Forceps (>10 µl)
- Grease (in single-channel units only)
- Instructions for use
- Performance certification in accordance with
ISO 8655-6
Please check that all items are included and that no
damage has occurred during shipment.
NOTE: The battery is protected against discharging
during storage with a plastic protector.
BEFORE USE PLEASE REMOVE THE PLASTIC
PROTECTOR UNDER THE BATTERY COVER.
NOTE: The pipette is supplied with discharged battery.
It is recommended to recharge the pipette for at least
12 hours before initial use.

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Battery Cover
Charging
Contacts
Reset Button
Finger Hook
Tip Cone
(5000 µl)
Tip Ejector
Assembly
(5000 µl)
Display
Keyboard
Tip Ejection Buttons
Start Button
Handle
Handle Locking Collar
MCP Connecting Collar
Tip Ejector Collar
(10 µl –1000 µl)
Tip Cone Housing
Tip Ejector Bar
Tip Cone(s)
(excl. 5000 µl)
Safe-Cone Filter
Operating Mode Symbol
Mode Range
Aliquots/Counter Volume
Speed in/out Battery Symbol
Aspirating/Dispensing direction
Display:
4. Pipette Description
4.1. Display and Controls
The control and programming of the eLINE® pipette is
performed using the display, keyboard and operating
buttons.

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NOTE: When the pipette is not connected to the
charging unit the pipette/display will automatically
switch off after 10 minutes of non-use. Pipette can
be switched on by pressing any key.
4.2. Reset Button
To reset the pipette, press the reset button on the
battery cover. The display shows RESET and PRESS TIP
in a blinking form and pressing the tip ejector button
will reset the pipette to the first programming level
in PIPET mode.
During the reset function the pipette automatically
determines the home position for the piston and tip
ejector by running the piston up and down. During the
reset function the display shows AUTO TEST and the
completed reset function is confirmed by showing the
text DONE on the display.
NOTE: The pipette must never be reset with the tip
attached! The attached tip can induce an error in
home position determination. However, if resetting
is accidentally done with the tip attached reset the
pipette again without the tip attached.
4.3. Start Button
The start button triggers the aspiration and dispensing
operations according to selected operating mode.
Only a soft touch is required to operate the button. If
the start button is held down during dispensing, the
piston will stop in the lowest position until the button
is released. In the operating modes with automatic
blow-out (P, dd) the piston will automatically return
to the home position after the start button is released.
If the start button is held down in the reverse pipetting
mode or during the dispensing of the last aliquot
in the multi-dispensing modes the piston direction
symbol will change within one second and the pipette
will be ready to aspirate the next sample when the
start button is released.

7
Keyboard:
Select Arrow up/down Enter
- Press to select - Press to select - Press to confirm
the mode range or the operating the selections or
to activate/confirm mode or to make setting changes
setting changes setting changes
(volume, aliquots,
speed, etc.)
Operating buttons:
Start button Tip ejection buttons Reset button
- Triggers all piston - Press to remove tip - Press to reset the
movements pipette
Mode ranges and operating mode symbols:
PROG
PROG 1-6
= 6 storage
locations
MULTI
d= multi-dispensing
dd = diluting
dd+MIXING = diluting
with mixing
Sd* = sequencial
dispensing
SA = multi-aspirating
Ad = automatic
multi-dispensing
*Not available in eLINE® 5 µl
PIPET
SP* = super pipetting
P= pipetting
rP = reverse
pipetting
P+MIXING = pipetting
with mixing
P COUNT = pipetting with
cycle counter
P MANUAL** = manual
pipetting
*Only available in eLINE® 5 µl
**Not available in multichannels

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4.4. Tip Sealing and Electronic Tip
Ejection
The pipette tips are recommended for use with
eLINE® pipettes. The eLINE® multichannel pipettes are
provided with a spring loaded OPTILOAD tip cones for
optimal tip sealing.
For easy and effortless tip ejection all eLINE®
electronic pipettes are equipped with the electronic
tip ejection feature. There are two tip ejection buttons
conveniently placed on the either side of the start
button to ensure easy reach for both right and left
handed users.
Tip ejection: When the pipetting cycle is completed,
either of the buttons can be depressed to eject the tip.
NOTE: The tip ejection function is frozen during the
pipetting cycle and can only be activated after the
tip is emptied. If needed the pipetting cycle can be
interrupted by pressing the enter button. The display
will show E (E=empty) and the tip is then emptied by
pressing the start button.
4.5. Safe-Cone Filters
The tip cone design of the eLINE® pipettes (>10 µl)
allows the use of Safe-Cone Filters in the tip cones.
These removable filters prevent liquids and liquid
vapours from entering the pipette and thus protects
the pipette from cross contamination and damage.
The Safe-Cone Filters are available as Standard or
Plus versions. It is recommended to use the Standard
Filter for general applications and the Plus Filter for
more demanding applications. The filters need to be
changed regularly. The interval for filter changing is
application dependant but the recommendation is
to change the filter daily (after 50 – 250 pipetting
cycles) and always in case of over-aspiration. The
filter forceps should be used to avoid touching the
soiled filters by hand. (See Chapter 2.1. for ordering
information.)

9
5. Charging of The Pipette
The battery is protected against discharging
during storage with a plastic protector.
BEFORE USE PLEASE REMOVE THE PLASTIC
PROTECTOR UNDER THE BATTERY COVER. However,
should the battery become discharged during storage
and recharging is required, a battery symbol will be
observed in the bottom right corner of the display.
The eLINE® pipettes should be charged using the
original Sartorius Charging Stand or Charging
Carousel. However, the eLINE® also features direct
charging by using the eLINE® AC-adaptor.
NOTE: If the battery is fully discharged, the display
will be empty and there will be no response from the
operating buttons. After a few minutes on charge,
the battery symbol will be displayed and the + and
– symbols will continue to blink until the battery is
fully charged.
5.1. Charging with the Sartorius
Charging Stand and Carousel
The charging stands and carousels are bench top
charging units. The charging stand accommodates one
eLINE® pipette and the charging carousel four eLINE®
pipettes. The stands and carousels operate through
the metal contacts placed in the sides of the pipette
and in the head of the charging units.
Before operating the AC-adaptor must be connected
to the socket at the rear of the charging stand or at
the side of the charging carousel as well as to the
electrical outlet. Before connecting the AC-adaptor
to the electrical outlet, check that its voltage settings
corresponds to the voltage settings of your electrical
outlet.
Cat. No. Product
730981 Charging Stand for one pipette
730991 Charging Carousel for 4 pipettes
With universal AC-adaptor (Euro, USA/JPN, UK, AUS, KOR and
China plugs)

10
1. Check that the battery protector is removed and
the battery cover is properly closed.
2. Place the pipette into the charging unit. Make
sure that the metal contacts at the rear of the
pipette are properly fitted to the tracks of the
metal contacts in the top of the charging unit.
3. When charging, the battery symbol will be
displayed with the + and – symbols flashing. The
charging time for completely discharged batteries
is 4 hours.
4. When the battery is full the battery symbol
disappears from the display. If display shows
RESET and PRESS TIP, press the tip ejection button
to reset the pipette, the pipette will then be ready
for use.
NOTE: Never reset the pipette with the tip attached
as the attached tip can induce an error in the home
position determination of the piston and tip ejector
collar!
NOTE: When the pipette is not in use it is recommended
to keep the pipette in the charging unit. This ensures
that the battery is kept charged and the pipette is
ready for use.
5.2. Charging Directly
The AC-adaptor supplied with the eLINE® pipette or
single charging stand can be used for the charging of
the eLINE® electronic pipette. The socket for the AC-
adaptor is situated on the back of the pipette.
1. Before connecting the AC-adaptor to the electrical
outlet, check that its voltage settings corresponds
to the voltage settings of your mains electrical
outlet.
2. Check that the battery protector is removed and
the battery cover is properly closed.
3. Connect the AC-adaptor to the mains electrical
outlet as well as to the socket at the back of the
pipette.
4. The charging time is 12 hours for a completely
discharged battery.

11
NOTE: It is possible to continue pipetting while the
eLINE® pipette is charging, connected to the AC-
adaptor. With a fully discharged battery a 30 minute
initial charging is required before continuing the
pipetting.
5.3. Electrical Specifications
Battery
- Rechargeable NiMH battery pack with protection
circuit
- 4.8 V, 500 mAH
- Charge empty batteries for a minimum of 12 hours
AC-adaptor for Charging Stand or direct charging
- Input voltages and main plug according to local
requirements
- Output voltage 7,5V DC, 800 mA
AC-adaptor for Charging Carousel
- Input voltages and main plug according to local
requirements
- Output voltage 9 V DC, 2000 mA
6. Programming Principle
The eLINE® pipettes features 10-11 different
operating modes and six different storage locations
for frequently used modes with user-selected settings.
The operating modes are divided in to three Mode
Ranges (PIPET, MULTI, PROG) and each Mode Range
includes the following operating modes:
Mode Range: Operating Modes:
PIPET SP, P, rP, P+MIXING, P COUNT,
P MANUAL*
MULTI d, dd, dd+MIXING, Sd, SA, Ad
PROG 1, 2, 3, 4, 5, 6 (=storage locations)
Programming is performed by using the Select, Enter
and arrow keys. The mode range is selected with the
Select key, the desired operating mode is then selected
with the arrow key and selection confirmed with Enter
key.

12
The setting changes (volumes, aliquots, etc.) are made
with arrow keys and confirmed with either Select or
Enter key. In the shortcut programming the changes
are confirmed with the Enter key. In the shortcut
programming the speed adjustment is not possible.
The use of the Select key in confirming the setting
changes enables also the speed adjustment.
NOTE: When the programming or pipetting cycle is
completed it is possible to make the setting changes
inside the operating mode simply by pressing the
arrow key and confirming with Enter or Select.
The eLINE® pipette includes also six storage locations
(Prog 1–6). The operating mode with user-selected
settings can be stored in to the storage location under
PROG mode for future recalls. After completing the
setting changes of the selected operating mode simply
press Enter and Select keys simultaneously, choose the
storage location (PROG 1-6) with arrow key and press
Enter. The stored operation mode can now be recalled
from the PROG mode.

13
or
or
Repeat until
programming
is complete
or
PIPET MULTI PROG
SP*d PROG 1
P dd PROG 2
rP dd + MIXING PROG 3
P + MIXING Sd*** PROG 4
P MANUAL** SA PROG 5
P COUNT Ad PROG 6
To change all settings To change settings When pre-stored
including speed: excluding speed: Prog 1–6 was chosen
Start pipetting
Store the
operating
mode with
Change selected
settings with settings to
Prog 1–6
+
Start pipetting +
Start pipetting Start pipetting
*SP is available only in eLINE® 5 µl
**P MANUAL is not available in multichannel models
***Sd is not available in eLINE® 5 µl
6.1. Programming map

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6.2. Speed Adjustment
There are 9 speeds available for both dispensing and
aspiration. The speed is indicated in the bottom left
hand corner of the display. The default speed is 5 for
all operating modes.
Speed can be adjusted during the programming by
completing the setting changes with the select key.
In every operating mode the speed adjustment is the
last programming step. After adjusting the “speed out”
the programming is completed with enter or select key
and the pipette is then ready for operation.
NOTE: The P MANUAL mode includes 5 different
dispensing and aspirating speeds.
7. Pipette Modes
The mode range PIPET includes 6 different operating
modes.
Symbol Description
SP Super Pipetting performs pipetting
with super blow-out function
enabling dispensing of very small
volumes from the air. SP-mode is
available only in eLINE® 5 µl pipette
PPipetting with automatic blow-out
rP Reverse pipetting without blow-out
P + MIXING Pipetting with blow-out followed
with mixing function
P COUNT Pipetting with blow-out and
displayed cycle counter
P MANUAL Manual pipetting with on-line
volume display

15
7.1. Super Pipetting (SP), Pipetting
mode (P) and Pipetting With
Mixing (P+MIXING)
1. Press to display PIPET
2. Scroll with until the symbol SP, P or P+MIXING
is displayed.
3. Confirm by pressing
4. Select the desired pipetting volume by using the
NOTE: When holding down the the volume display
starts to scroll faster.
5. Press to confirm selection and to display the
speed in or press and continue from Step
10.
6. Select the speed in with (1= Min and 9 =
Max)
7. Press or to confirm selection and to
display the speed out
8. Select the speed out with (1= Min and 9 =
Max)
9. Press or to confirm selections
10. Position the tip to aspirate and press the START
button
11. Position the tip to dispense. Press the START
button to dispense the sample. The tip is
emptied with blow-out and the piston returns
automatically back to the home position.
NOTE: When the START button is held down during
dispensing the piston will stop in the lowest position.
When the start button is released the piston will
automatically return to the home position.

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To mix:
1.
Position the tip in to the solution, then press and
hold the START button .
Mixing is performed automatically as long as the
START button is held down.
2. Position the tip to dispense. Press START button
twice
NOTE: The mixing is performed with about 70% of the
total volume.
7.2. Reverse Pipetting (rP)
A selected volume plus and excess is aspirated into the
tip. After dispensing the selected volume the excess
volume remains in the tip and is discarded.
1. Press to display PIPET
2. Scroll with until the symbol rP is displayed.
3. Confirm by pressing
4. Select the desired pipetting volume by using
NOTE:When holding down the volume display
starts to scroll faster.
5. Press to confirm selection and to display the
speed in
or press and continue from Step 10.
6. Select the speed in with (1= Min and 9 = Max)
7. Press or to confirm selection and to
display the speed out
8. Select the speed out with (1= Min and 9 =
Max)
9. Press or to confirm selections
10. Position the tip to aspirate and press the START
button
11. Position the tip to dispense. Press the START
button

17
12. Position the tip to discard excess liquid and press
the START button twice
NOTE: It is also possible to continue to aspirate and
dispense the same volume without the empty function.
To continue, keep the START button depressed during
dispensing and within one second the direction arrow
will change. Continuing to hold the START button
down, place the tip into the liquid again and then
release the START button to aspirate the next sample.
7.3. Pipetting With Cycle Counter
(P COUNT)
The eLINE® performs pipetting with blow-out and
the cycle counter function. The cycle counter counts
up to 99 pipettings and then returns back to 0. It is
possible to choose the cycle number to begin with. It is
also possible to change the cycle number or reset the
counter during the pipetting by using the arrow keys.
1. Press to display PIPET
2. Scroll with until the symbol P and COUNT is
displayed.
3. Confirm by pressing
4. Select the desired pipetting volume by using the
NOTE: When holding down the volume display
starts to scroll faster.
5. Press or to confirm selection.
6. Cycle counter will show 1. Use to select the
sample number you want to begin with (0-99).
7. Press to confirm the selection and to display
the speed in
or press and continue from Step 12.
8. Select the speed in with (1= Min and 9 = Max)
9. Press or to confirm selection and to
display the speed out

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10. Select the speed out with (1= Min and 9 =
Max)
11. Press or to confirm selections
12. Position the tip to aspirate and press the START
button
13. Position the tip to dispense and press the
START button . The tip is emptied with blow-
out and the piston returns automatically back to
the home position. The pipette is ready for next
pipetting.
NOTE: To change the cycle number or to reset the
counter during the pipetting, press the to display
the desired cycle number and confirm with .
7.4. Manual Pipetting (P MANUAL)
(Not available in multichannel models)
In the manual pipetting mode the aspiration and
dispensing are controlled manually. The aspiration
or dispensing continues as long as the START button
is held down (within the selected volume range).
The aspiration/dispensing direction can be changed
during pipetting by using the arrow key. In the manual
pipetting mode the display always shows the actual
amount of sample in the tip.
1. Press to display PIPET
2. Scroll with until the symbol P MANUAL is
displayed.
3. Confirm by pressing
4. Select the desired pipetting volume by using
NOTE: When holding down the volume display
starts to scroll faster.
5. Press to confirm selection and to display the