Shimadzu Shim-pack GIST-HP Series User manual

ERAD-0001-9011B
Guard Column for Ultra High Performance Packed Column
GIST/GISS/GIS -HP (G) Series
Shim-pack
INSTRUCTION MANUAL
Introduction
This product is manufactured for the purpose of protecting
analytical columns from contaminants in samples and eluents.
This cartridge type provides easy installation and replacement.
Please read the following instructions before use.
This product contains Holder A, Holder B, Tubing (0.18 mm I.D. x
20 mm) , male nut and ferrule.
Operating Precautions
Check if anything is missing or damaged. If there are any signs of
damage, notify your local Shimadzu representative at once.
Column Installation
Refer to the following instructions to install the Guard Columns for
UHPLC properly.
1. Check the flow direction of the cartridge guard column, and
then insert the cartridge into the holder B.
2. Hand-tighten the holder until it no longer turns easily.
Retighten the holder from 60 to 90 degrees by using a 10 mm
spanner.
3. Connect the inlet set of the holder prepared at step 3 to the
outlet side of the injector.
Besides the male nuts for the analytical column, another set of
male nuts are necessary to connect the Cartridge Guard
Column. Ensure that the fittings are connected properly to
avoid creating dead volume between the tubing and the guard
column interface. Male nuts can be ordered by referring to the
part number below.
Use a 0.1 - 0.25 mm I.D. tubing to minimize extra column
volume. Use the shortest possible tubing connection to
minimize extra column volume.
4. Use a 10 mm spanner to connect the guard column to the
analytical system.
5. This product is shipped under dry conditions and contains air
bubbles. Make sure to remove air bubbles from the cartridge
before use. Set the flow rate to 0.1 - 0.2 mL/min for 1.5 mm
I.D., 0.2 - 0.4 mL/min for 2.1 mm I.D, and 0.4 - 0.8 mL/min
for 3.0 mm I.D. guard columns.
6. To connect the guard column to the analytical column after
confirming air bubbles are completely removed, tighten the
male nut or UHPLC fitting by using a spanner. The size of the
tubing attached to the outlet side is 20 mm x 0.18 mm I.D.
Attention
Shape of the column joint is a lot of variation (HSS
type, Waters type) depending on the kind of column.
It might be the case that the combination of the
column joint have negative influence on the
separation. Therefore, pay attention to the points
below when use the ferrule and the male nut.
•In the case of using the male nut and ferrule
enclosed in the box, please tighten these with use of
column which will be connected with after insert the
male nut and then the ferrule in the tubing.
•In the case of using various vender’s column,
recommend to use the UHPLC fitting (S228-56867-
41) which the position for tighten can be freely
moved in order to avoid the dead volume as long as
it can. UHPLC fitting is not including in this
product. UHPLC fitting can be ordered separately.
7. Flush the column with mobile phase and inspect for leaks. In
case of any leakage, check all connections and retighten the
holder using a 10 mm spanner.
Item name P/N Remarks Pressure
Male nut 1.6 MN 228-16001 1 pc 130 MPa
Ferrule 1.6 F 228-16000-10 1 pc 130 MPa
UHPLC Fitting 2 S 228-56867-41 1 pc 130 MPa
Tubing
0.18 mm I.D. x 20 mm
Holder B
Column Connection
Connection to
Analytical column
Cartridge
Guard Column
Holder A
Male
Nut
Male nut,
Ferrule

* The contents of this instruction sheet are subject to change without notice.
Column Handling Precautions
To maximize column life operate at pressures below 80 MPa.
Storage of Columns
When storing the guard column for a long period, remove the
cartridge from the holder and store it under dry conditions.
Store the guard column/holder/cartridge in a cool and dark place.
Technical Support
This product is manufactured, inspected, packaged and shipped
under strict standards of quality control. Should you find any defect
in performance, please contact your local Shimadzu representative,
who will ensure your complete satisfaction.
We regret that we cannot guarantee the lifetime of columns, also
that we cannot accept any claim when performance has deteriorated
due to noncompliance with the operation procedures elucidated
above, or as a result of normal aging.