Telstar MINI-V/PCR User manual

USER'SMANUAL
VerticalFlowBenches
MINI-V/PCR
™" ?«B UM
MINI-VI rev0507US
Teistar Industrial, S.L.
JosepTapiolas, 120
08226TERRASSA(Spain)
Tel. [phone]
Fax: [phone]
[email protected]m
www.etelstar.com

MINI-V_I_rev0507USPage2
INDEX
1.
DESCRIPTIONOFPRODUCT
2. GENERALDETAILS
2.1.
2.2.
2.3.
2.4.
2.5.
WorkingPrinciple
Applicationsandworkingdiagram.
Descriptionofcomponents
Certification
TechnicalFeatures
3.TRANSPORT
4. INSTALLATIONINSTRUCTIONS
4.1.
4.2.
4.3.
4.4.
4.5.
4.6.
4.7.
Placement
Firstconnectionprecautions
Electricsupplyconnection.
Start-up
Switchingoff
Longstop.
U.V.sterilizationstart-up.
5.USER'SINSTRUCTIONS
6.
5.1.
5.2.
5.3.
5.4.
6.1.
6.2.
6.3.
6.4.
6.5.
Generalrecommendations
CONTROLPANELANDALARMS
Standardaccessories
Optionalaccessories
MAINTENANCE
Maintenancetable
Periodiccontrolandcertification
Pre-filter
Absolutefilters.
Spares
7.TROUBLESHOOTING
8.CLEANINGANDDISINFECTION
8.1. Surfacecleaninganddisinfection
8.2.Maintenancechart
9.GUARANTEECERTIFICATE.
10. CECERTIFICATION
5
5
6
6
6
6
6
.6
.7
7
8
9
.9
11
11
11
11
.11
12
12
12
12
13
14
15

MIIMI-V_I_revO5O7USPage 3
1. DESCRIPTION OF PRODUCT
InstructionsManual for VerticalLaminarFlowCabinetensuringhighsampleprotection.
Model: MINI-V/PCR
WARNING
It is essential to read and understandthisManualbeforegoingaheadwithVerticalLaminarFlow
Cabinetstart-up.
All the instructions in thisManualmust be obeyed.
Beforecarrying outany Maintenancework onthe cabinet,switch itoff from the source of
supply.
Any useor application notin keepingwith the recommendationsgiven in thisManual may
createrisk and meanloss of right to manufacturer'sguaranteeexempting the latterfrom all
liability.
MANUFACTURER:
TELSTARINDUSTRIAL, S.L
JosepTapiolas, 120
E-08226 - TERRASSA
Telf. [phone]
Fax.[phone]
Web: www.etelstar.com
E-mail:[email protected]m
2. GENERAL DETAILS
2.1. WORKING PRINCIPLE
MINI-V/PCRverticallaminarflowcabinets are equippedwith a highfrequencycentrifugal
fan.
Theoutsidecabinet is made of stainlesssteelsheet,lacquered and ovendried, of functional
design and optimumdimensions.
Outside air taken in(1) passesthrough a pre-filter (2),is sucked bya fan(3), impelled
towards a plenum,passedthrough a HEPAabsolutefilterand, as laminarflowenters the
workarea,reachesClass 100 according tous Fed. Std.209D; then itis forced outbyit's
highpressure (7) Fig.l.
2.2. APPLICATIONS AND WORKING DIAGRAM
Thisverticallaminarflowcabinet is suitable for dealingwithsamples or productswhich
involve the need for sterile air conditionsaccording to Class 100inthe workarea, but where
neitheroperator nor environmentalprotection is necessary. For principle see Diagram in
Fig.l.

MINI-V_I_rev0507USPage4
WARNING
Under no circumstances canit work in environmentscontainingcorrosive,inflammable or
explosive gas.
Under no circumstances can it workwithproducts or samples,which are biologicallypathogenic
orliable to affect the operator or environment in anyway whatsoever.
Under no circumstances can it workwithcytostatics(chemotherapy) or radiologicsamples.
Fig. 1
Cabinetcomponents.
2.3. DESCRIPTION OF COMPONENTS
Fig. 1.
1 Outside air inlet.
5
HEPAabsolutefilter.

MINI-V_I_revO5O7USPage 5
2
3
4
Blanketpre-filter.
Centrifugalfan.
Chamberorplenum.
6
7
Workingarea.
Airoutlet.
2.4. CERTIFICATION
Qualitycontrolandcertificationwithresultsoftestsperformedaccordingtonorms.
Individualcertificateinsideeachcabinet.
2.5. TECHNICAL FEATURES
PREFILTER
FILTER
FAN
LIGHTING
NOISELEVEL
AIRFLOW/SPEED
OUTSIDEDIMENSIONS
(lengthx widthx height)
INSIDEDIMENSIONS
(lengthx widthx height)
POWER/WEIGHT
SUPPLY
G380-85%ASHRAESyntethicfibrefilteringbanket
D.O.P. > 99,999%EfficiencyHEPAfilters for CLASS
100 H-14
Highefficiencycentrifugal or doublesuction fan.
>550 lux
< 56dBa
400 m3/h- 0,40m/s
670 x 520x 1125mm.
630 x 500x 570mm.
0,4 kW- 70Kg
Seethechnicalplate on cabinetfront.
3-TRANSPORT
Eachcabinetispackedindividuallyandsuitablyprotectedagainstdamageduringtransport.
ThisManualandindividualcontrolcertificateforeachindividualcabinetissuppliedaspartof
theconsignment.
4.INSTALLATION INSTRUCTIONS
Toensurecorrectverticallaminarflowcabinetstart-up,thefollowinginstructionsshouldbe
followedstepbystep.
4.1. PLACEMENT
Specialcaremust be takenwhenunpacking the microprocessorscreen.
Place inan areaawayfromdoors,windows andair currentsfrom air conditioners and where
there is littlemovementcaused by people, to avoiddraughts.
Thecabinetmust be perfectlysteady onits table or bench to preventvibration.
Topreventoverheating the workarea, itis advisable to keeproomtemperaturewithin the
limits of workcomfort.

feTElSTAR
MINI-V_I_rev0507USPage6
4.2. FIRST CONNECTION PRECAUTIONS
Beforeconnecting tothe mains,carry out generalcleaning in order to removedustparticles
accumulatedduringtransport. Todo this,followcleaning and disinfectinginstructions.
Earthsuitablyaccording tothe cabinetplatedetails.
Beforeworking forthe firsttime,start the cabinet andletitrunfor about 6 hours to purge
out the filters.
Newfiltershave a characteristicsmell,whichusuallydisappears ina shorttime.
4.3. ELECTRIC SUPPLY CONNECTION.
Thecabinet is suppliedwith a singlephase fanand connectingcablewithEuropean (or other
asspecified) plug.
Theelectroniccardincludesprotectionfuses.
Thegeneralswitch isatthetopofthe cabinet, onthe rightseenfromfront, bythe electric
cable.
WARNING
Beforeconnecting the cabinet,checkthat the supplysourcecoincideswiththatshown
on the specificationplate.
Theinstallationconnectedmustcomplywith the safetyrequirements of the country
where itis found.
4.4. START-UP
Once the previouspointshavebeencovered,start the verticallaminarflowcabinet by
pressingbutton (Fig.2 pos.1)and makesure the suctionarea (Fig.1. pos.l) is free.
4.5. SWITCHING OFF
Tostop the cabinet,turn the button(Fig.2pos.l) to stopposition, and stop the general
switch atthetopofthe cabinet.
4.6. LONG STOP.
When the cabinet is idle fora longtime,avoid, asfaras possible,entry of dust and when
renewingwork, the
procedureshould beasifit werefirstconnection. See paragraphs 4.2and4.4 (above).
4.7. U.V. STERILIZA TION START-UP
This is started by pressingbutton (Fig.2 pos.3), whichturns onthered alarm (Fig.2
pos.7). To changepresetsterilizationtime, see5.2 Control and alarmpanel(below).
WARNING
Ifthisaccessory is used, the usualprecautions for theselampsmust be taken. Itisto
beusedonly for sterilizingmaterialoutsidebefore it enters the cabinet or,allthe
materialusedduring the previous PCR tobe re-used. In this way, the lamp isa
supplement for workbefore itis done inthe cabinet, and must beoff when the
operator is facing the table or working atit. Direct UV radiatedrays are reflected on
thepolishedmetalparts andcan seriouslyaffect the operator'seyes.

MINI-V_I_rev0507USPage 7
5.USER'SINSTRUCTIONS
5.1. GENERAL RECOMMENDATIONS
Theworkarea ofthe cabinetshould notbe used asfor storinglaboratoryequipment.This
couldproduce an accumulation of dustwhich is bothunnecessary and dangerous for
working in sterileconditions.
Even ifthe workarea is undercleansterilelaminarflow,thatsurrounding the cabinet can
becontaminated. The sources of contaminationshould be known so thatthey canbe
avoidedwheninsertingarms,material etc.
All the materialnecessary for workmust be free of particles and cleanedbefore it enters.
Materialslike:paper,wood,cardboard,pencils,erasingrubbers, etc., should notbe inserted
asthey all shedlargeamounts of particles.
Bothbefore and afterwork, itis advisable to washarms,hands and fingernailswith
germicidesoap.Personnelshouldavoidtouchingtheirmouths and eyes.
Longsleevedwhitecoats are advisedwithtightcuffs, and,for specialwork,protecting
gloves, (or alternatively,over-sleeves).Both the whitecoats andthe over-sleevesmust be
made of materialsleastlikely to shedfibre and particles.
Thecabinetshould be started 10 minutesbeforeworkbegins.Thissweeps the workarea
andmaterialinserted of particles.
Ifpipettes are used,theyshould be mechanicsuctiontype;suction by mouthmustnever
beused asitis easy to inhaleaerosols so caused.
Whenplatinumhandlesmust be used,electricincinerators or, better still, disposablesingle-
usehandles are advised.
If the nature ofthe work tobe doneinvolvesusing a gas flamefrom a Bunsen (or similar)
burner, the buttonopeningconstantpressuretype is advised. It must be pointed out that a
constantflameburnercreates a considerableamount of turbulence.Rememberthat too
large a flame can burn the absolutefilters.
Shadows and turbulenceproduced by objects,equipment and material inthe workarea,
must be takenintoaccountbeforeworkbegins to assesstheirpossibleeffect. It should be
rememberedthat air flowlaminarity is onlyrecovered ata distance ofupof2.5 times the
diameter ofthe objectcausing the obstruction.
Ifflasks or tubes are used,theseshould,preferably, be screw cap ratherthancotton
stopped, asthe lattershedslargeamounts of particles.
Assoon asthe workfinishes, allthe disposableproducts,such as: handles,Petriplates,
etc., as well as means of culture,samples,tubes,flasks andthe like, aretobe taken outof
thecabinet and emptiedintoimpermeablebags,which canbe sterilized if necessary.
Duringwork inthe cabinet and cleaning,caremust be taken notto damage the HEPAfilters
byknocking,squirtingliquid ator splashing it.

IDTEISTARMINI-V_I_rev0507USPage8
5.2. CONTROL PANEL AND ALARMS
Descriptionofthecomponents(Fig.2).
Thicknessofthecontrol dial: 3 mm.
2x16LCDdisplaywith3x5.5mmcharactersforthedifferentindicationsandprograms.
Sixbuttons.
Controldialfunctions:
-Button(1):connectsthefan,typeon/off.Bydefault,thefanspeedissetatstandards,ifyou
wishittogoslower,youshouldthenpressthedownbutton(n°6).
-Button(2):connectsthelighting,typeon/off.LocatedwiththeUVgermicidallampbutton(n°
3).
-Button(3):connectstheUVgermicidallamp,typeon/off.Locatedwiththelightingswitch(n°
2).
ToconnecttheUVlampwitha timer,youshouldpressthebutton(n°3)andstartit
up. Thebutton(5)activatesordisactivatethetimer.
StepstofollowtoprogramthetimerfortheUVlamp:
1.- YoushouldstartwiththeUVlampswitchedoff.
2.- Pressthedownbutton(n°6).
3.-Selectthetimedesired,from1 to59minutes,usingtheupanddownbuttons(n°
5/n°6).
4.- ConfirmwiththeUV'sstartbutton(n°3).
-Button(4):connectstheAUXoutputforpossiblesafetyvalves,serviceplugs,etc.
Signallights,alarmsanddisplay:
Signallight(no.7)forair-impulsionsystemfunctioning,green.
Signallight(no.8)forlightingswitchedon,blue.
Signallight(no.9)forUVgermicidallampswitchedon,yellow.
OPTIONAL.Filtercloggingalarm(no.11),red.
UVgermicidallampon(no.12),red.
Displayfunctions:
-ThedisplayusuallyshowsTELSTARandtheversionoftheprogram.
-ViewerforhoursofairimpulsionsystemandUVgermicidallampfunctioningV.
(VENT/FAN: OOOOOOh,U V:OOOOOOh)willbeactivatedwiththebutton5,being
therestoffunctionsstopped.
-Normalorslowfanspeed(velocity)(FANVELSTDorALARMFANVEL1/2).
-Slowspeedairimpulsionalarmsystem(ALARMVEL.1/2).
-FixorprogrammedperiodoftimeforUVgermicidallamp(ALARMUVONorUVON
12MIN).
-UVlampalarmon(ALARMU.V.ON).
-Filtercloggingalarm(ALARMFILTER).

MIIMI-V_I_rev0507USPage 9
Fig. 2
5.3.STANDARDACCESSORIES
Thefollowingaccessories are installed in verticallaminarflowcabinets:
- 3A- 600W. electricplug.
- U.V. germicidelamp kit.
5.4. OPTIONAL ACCESSORIES
- Gas cock/tap(Fig.3).Reference: 11253
-Vacuumcock/tap.(Fig.3).Reference:11220
- Compressed air cock/tap(Fig.3).Reference 11254
WARNING '
Ifcompressed airor nitrogen are used,careshould be taken to install an absolutefilter inthe
pipeline to preventparticlesgettinginto the workarea.
Thecabinet is prepared forthe user to install a verysimple tap(see instructions inFig.3).If
morethan one sourceconnection is needed, itcanbe requestedwith the order asitcan only be
made inthe factory.
- (U-15)D.O.P.99.999%efficiencyHEPAfilter for class 10 installed,withparticlecount
certificate.
- PolycarbonateDoors.

MINI-V_I_rev0507USPage10
ForprotectionagainstdirectUVrayradiation.
-Pipettefasteningsupport:Reference11289
Aselfstickingstainlesssteelpart to placeinside the cabinet ina positionchosen bythe user.
Dimensions:U 30x25mm280mm long.
Fig. 3
MountingTap in Cabinet
A.-Removeplugs (1) and (2).
B.-Removethetwoscrews (3) outandcover(4).
C-Placetap (5)inthe insideworkchamberandscrew itupto joint (6) insertedfromoutside
thecabinet.Beforethis,soakthethread ina fixingliquid(Loctite - 222).
D.-Putthecover(4)backandscrewupthetwoscrews(3).

MINI-V_I_rev0507US Page11
6. MAINTENANCE
WARNING
Before embarkingonanymaintenanceorcabinetdismountingwork,switchcabinetofffrom
source.
As a general rule, technicalspecialistsshouldmakeanannualcheckthatallcabinetcomponents
function correctly.
6.1. MAINTENANCETABLE
Thefrequencydeterminedforeachoperation,andparticularlythatforchangingfilters,
shownontheMaintenanceTablebelow,areforguidanceasadvisedfornormalcabinet
service; verysevereworkconditionswillmeanreducingtheintervalsshownontheTable.
MaintenanceTable
OPERATION(JOB)
Regularcheckandcertificationaccordingto
Norms.
Pre-filtercleaning
Pre-filterreplacement.
Absolutefilterreplacement
U.V.lampreplacement
PERIOD
Atleastoncea year.
Every500workinghours.
Every1000workinghoursoncea year.
Every3000/ 4000workinghours.
Every7000workinghours.
6.2. PERIODIC CONTROL AND CERTIFICATION
Internationalnormsrecommendcertificationandcontrolperiodically(atleastoncea year).
Theteststobecarriedoutarethesameasthoseusedduringthefinalmanufacturing
processandcertificatesareissuedontheresults,asperNorms.
AttheendofthisManual,thereisa tabletousetodrawupa maintenancehistory.
6.3. PRE-FILTER
Intheairsuctionoftheverticallaminarflowsterilecabinet,thereisa pre-filter(Fig.lpos.2).
Every500hoursitshouldbecleanedorvacuumcleanedandevery1,000hoursreplaced.
WARNING
Under nocircumstancesmustthecabinetbestartedwithoutcorrectlyinstalledpre-filter,asthis
will causerapidsiltingupoftheHEPAfilter.
6.4. ABSOLUTEFILTERS
The averagelifeoftheHEPAfiltersisshownontheMaintenanceTable(6.1).
After this period, theflowofimpelledairdecreasesduetogreaterlossoffilterloadsince
filtering ismechanical;butthisdoesnotmeananylossoffilteringefficiency.

feTElSTAR
MINI-V_I_rev0507USPage12
WARNING
Inorder to ensurecorrectfilterinstallation and adjustment, and alsoabsence of leaks,filter
replacementmust bea matter for specialisttechnicalpersonnelsuppliedwith the necessary
controlinstruments:anemometer,leakdetector,aerosolgenerator,particlecounter etc.
TelstarIndustrial S.L representatives and distributorshave a qualifiedtechnicalteamwith the
mostup-to-date,healthprotectingcontrolequipment to carry out periodiccontrolswith
guaranteedsafetyandalsochangefiltersaccording to the Norms.
6.5. SPARES
Ref.
1558
11246
11217
1338
1533
15463
15462
Description
Impelling fan and condenser
HEPA610x457x69mm. H14 HEPAfilter
ASHRAE G3 - 80-85%syntheticblanketpre-filter
30Wfluorescentlamp
15Wgermicidelamp
Controlpanel
CPUelectroniccard
7.TROUBLESHOOTING
Problem: Motorstops.
Possiblecause:Fuseblown.
Solution: Find out cause of fusing.Whenthis has beensolved,changefuse.
Problem: Contaminationdetected in workarea.
Possiblecause:Absolutefilterperforated; ithas comeawayfrom its frame;sealingjoint
betweenfilterandcabinetbaseineffective.
Solution: A D.O.P.leaktest is carried outbya specialisttechnician to discovercauses of
contamination and absolutefilter is replaced by him, if necessary.
Ifcontaminationcontinuesafter thenew absolutefilter is installed and freshairtightness
(D.O.P)tests are carried out, the usershouldstartlookingintocorrectapplication of work
procedures, as there maybe contaminationfrom an outsidesource,throughworktools,
hands or the samples.
8. CLEANING AND DISINFECTION
8.1. SURFACE CLEANING AND DISINFECTION
Toclean the worktableandsides,sterileclothswill be usedwhichshed no particles or fibres.
Todisinfect,theseclothswill be usedslightlydampenedwith a disinfectantsolution,whichwill
notaffect the paintlacquer or thestainlesssteel.

MINI-V_I_rev0507USPage13
Werecommendinsidecleaning of the cabinet onthe followingoccasions:
-Beforebeginningwork in the cabinet
-Whenwork has finished
-Whenever a workprogramme is changed
- If liquid has beenspilt onthe worktable
-Beforecarrying out a controltest.
8.2. MAINTENANCE CHART
DATEREMARKS DONE
BY
SIGNATURE

MINI-V_I_rev0507USPage14
9. GUARANTEE CERTIFICATE
TELSTARINDUSTRIAL,S.L guaranteesthesubstitutionFREEOFCHARGEofallthepieces
sufferingbreakdownintheperiodofONEYEAR,startinginthedateoftheexpeditionofthe
equipment.
Thisequipmenthasundergonesatisfactorilyallthetestsandrevisions.Therefore,any
damagecausedbytransport,wronginstallationorinappropriateuseisexcludedofthis
guarantee.
Thisguaranteedoesnotincludeanyofthefungiblematerialscomprisedintheequipment,
suchasfilters,fluids,filaments,etc.
Theinappropriateuseofthisequipmentprovokestheguaranteetobe void. Thisinvolvesthe
exclusionofanyliabilityinconceptofindemnityforeventualdirectorindirectdamagesof
anynature,eithermaterialorpersonal.
Likewise,thisguaranteewillbevoidifpiecesotherthantheonessuppliedbyTELSTAR
INDUSTRIAL,S.L.areusedfortherepairingoftheequipment.
NOTE. Thisguaranteeisvalidforalltheequipmentunlessotherwise
stated. Inordertoobtainanyinformationaboutthisguarantee,please
contactwithourQualityAssuranceDepartment.

MINI-V_I_rev0507USPage 15
10. CE CERTIFICATION
DECLARACION DE CONFORMIDAD
DECLARATION OF CONFORMITY
Estecertificado se emitepara el aparatoelectrico/Thiscertificate is issued forthe electrical
apparatus:
CABINAESTERIL POR FLUJOLAMINARVERTICAL
VERTICAL LAMINAR FLOW CLEAN BENCH
MODELO/ MODEL: MINI-V/PCR
Fabricada y sometida a Certificacion por TELSTARINDUSTRIAL, S.L./ Manufactured and
submitted for Certification by TELSTARINDUSTRIAL, S. L.
91/368/EEC y 93/68/EECDirectivasSeguridad en Maquinas/Machinery Safety
Directives.
Normativarespecto a la cual se declaraconformidad/Standard(s) to which conformity is
declared: EN 292-1, EN 292-2, EN294,EN 418, EN348
73/23/EECDirectivaSeguridadElectrica/Electrical Safety Directive.
Normativarespecto a la cual se declaraconformidad/Standard(s) to which conformity is
declared: EN 61010-1(Requisitos de seguridad de equiposelectricos de medida,control y
uso en laboratorio/Safety requirements for electrical equipment for measurement, control
and laboratory use).
89/336/EECDirectivaCompatibilidadElectromagnetica/Electromagnetic Compatibility
Directive.
Normativarespecto a la cual se declaraconformidad/Standard(s) to which conformity is
declared: EN50081, EN 50082-2 (61000-4-2/3/4/5/6/11),EN 55011 ,55022B,EN
61000-3-2/3, 60555
YNormas de Construccion/M/idManufacturing Standards:
U.S.Fed.Std.209, B.S. 5295
El anode marcajeCE fueen 2000/The CEmarking was affixed in 2000.
Por la presente,TELSTARINDUSTRIALdeclara queel equipoarribaespecificadocumple
con las Directivas y Normativasarribamencionadas,siempre y cuando la instalacion sea
acorde a las especificaciones del fabricante.
TELSTAR INDUSTRIAL hereby declares that the equipment specified above conforms to
the above Directives and Standards, when installed in accordance with the manufacturers
specifications.
MerceGuilera
QualityAssurance
Terrassa May 2007

MINI-V_E_rev0507USPag.2
INDICE
1.DESCRIPCIONDELPRODUCTO
2. DATOSGENERALES3
2.1. PrincipioFuncionamiento3
2.2. Aplicacionesy esquemadefuncionamiento3
2.3. Descripciondeloscomponentes4
2.4. Certificacion.5
2.5.Caracterfsticastecnicas5
3.TRANSPORTE5
4. INSTRUCCIONESDEINSTALACION6
4.1. Emplazamiento6
4.2. Precaucionesenlaprimeraconexion6
4.3. Conexionelectricay deservicios6
4.4. Puestaenmarcha• 6
4.5. Desconexion7
4.6. Paroprolongado7
4.7. PuestaenmarchaesterilizacionU.V.7
5.INSTRUCCIONESDEUSO7
5.1. Recomendacionesgenerales7
5.2.Caratulademandos8
5.3.Accesoriosstandard9
5.4.Accesoriosopcionales9
6.MANTENIMIENTO11
6.1. Tablademantenimiento11
6.2.Controly certificacionperiodicos11
6.3.PrefiItro11
6.4.Filtrosabsolutos12
6.5.Recambios12
7.ANOMALIASDEFUNCIONAMIENTO12
8.LIMPIEZAY DESINFECCION13
8.1. Limpiezay desinfeccionsuperficial13
9.HISTORICODEMANTENIMIENTO14
10. CERTIFICADODEGARANTIA15
11. CERTIFICADOCE16