Thermo Scientific 1300 Series User manual

1300 Series A2
Class II, Type A2 Biological Safety Cabinet
Operating Manual 7021355 Rev. 18
User Manual
Visit us online to egiste you wa anty
www.the moscientific.com/labwa anty

Thermo Scientific
Preface
1300 Series A2 i
This manual covers the models shown below:
Dear User,
ongratulations on your purchase of a Thermo Scientific 1300 Series lass II, Type A2 biological safety cabinet! Your 1300 Series A2 biological safety
cabinet has been tested and certified in accordance to NSF/ANSI 49, and is designed to protect the user, the environment, and your research from
harmful substances and cross-contamination. This user’s manual provides instructions on how to use the 1300 Series A2 most effectively and safely. A
printed copy of this user’s manual is available from Technical Services, if required.
Containment * Comfort * Convenience™
The 1300 Series A2 offers a unique range of product features that will enhance your safety and improve overall operating efficiency. Should you have
any questions on using this product or need further explanation of any of it’s features, please contact Technical Services (Page iv), or your local
distributor.
Stainless Steel Interior Coated Interior Size Voltage
10 inch opening 8 inch opening 10 inch opening 8 inch opening
1322 1341 1320 1339 3 ft 120V
1323* 1342* 1321* 1340* 3 ft 120V
1345 1305 1355 1325 4 ft 120V
1346 1306 1356 1326 4 ft 230V
1354 4 ft 230V, China
1384* 4 ft 230V, China
1370 1352 1368 1350 5 ft 120V
1371* 1353* 1369* 1351* 5 ft 120V
1347 1307 1357 1327 6 ft 120V
1348 1308 1358 1328 6 ft 230V
1359 6 ft 230V, China
1389* 6 ft 230V, China
1332 1392 1330 1390 3 ft 230V
1333* 1393* 1331* 1391* 3 ft 230V
1375* 1335* 1385* 1395* 4 ft 120V
1376* 1336* 1386* 1396* 4 ft 230V
1382 1366 1380 1372 5 ft 230V
1383* 1367* 1381* 1373* 5 ft 230V
1377* 1337* 1387* 1397* 6 ft 120V
1378* 1338* 1388* 1398* 6 ft 230V
* Includes cabinet, adjustable eig t stand, UV lig t and armrest.
MANUAL NUMBER 7021355
18 ECNM-001017 06/24/2020 Added Maintenance Notes in page 6-1. TFI
17 ECNM-000187 12/03/18 Updated inflow test description and factors TFI
16 41783 11/20/17 Corrected Downflow and Exhaust air dimensions on pages 7-6 & 7-7 IEC
15 41700 8/2/17 Added Drain valve to Fig 1-1, 2-6 bpg
14 40072 7/21/17 Clarified shutdown instructions bpg
13 41335 12/12/16 Added note to Drain Valve installation on page 2-8 ccs
12 40525/ D-1941 9/1/16 Noted SmartPort as option ccs
11 31809 4/6/16 Updated operating hours of the device to filter life meter (pg 1-5) ccs

Thermo Scientificii 1300 Series A2
Preface
Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. s
Caution All internal adjustments and maintenance must be performed by qualified service personnel. s
Material in this manual is for information purposes only. The contents and the product it describes are subject
to change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this
manual. In no event shall Thermo Fisher Scientific be held liable for any damages, direct or incidental, arising
out of or related to the use of this manual.
© 2020 Thermo Fisher Scientific. All rights reserved.
Contains Parts and Assemblies
Susceptible to Damage by
Electrostatic Discharge (ESD)
CAUTION

Thermo Scientific 1300 Series A2 iii Thermo Scientific
Preface
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.
Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be
damaged by elevated temperatures.
WEEE ompliance: Thermo Fisher Scientific has contracted with companies for recycling/disposal in
each EU Member State. For further information, send an email to [email protected].
4Always use the proper protective equipment (clothing, gloves, goggles, etc.)
4Always dissipate extreme cold or heat and wear protective clothing.
4Always follow good hygiene practices.
4Each individual is responsible for his or her own safety.

Thermo Scientific
Do You Need Information or Assistance on
Thermo Scientific Products?
If you do, please contact us 8:00 a.m. to 6:00 p.m. (Eastern Time) at:
1-740-373-4763 Direct
1-800-438-4851 Toll Free, U.S. and Canada
1-877-213-8051 FAX
http://www.thermofisher.com Internet Worldwide Web Home Page
[e-mail] Tech Support Email Address
Certified Service Web Pagewww.unitylabservices.com
Our staff can provide information on pricing and give you quotations. We canSales Support
take your order and provide delivery information on major equipment items or make
arrangements to have your local sales representative contact you. Our products are listed on the
Internet and we can be contacted through our Internet home page.
Our staff can supply technical information about proper setup, operation orService Support
troubleshooting of your equipment. We can fill your needs for spare or replacement parts or
provide you with on-site service. We can also provide you with a quotation on our Extended
Warranty for your Thermo Scientific products.
Whatever Thermo Scientific products you need or use, we will be happy to discuss your
applications. If you are experiencing technical problems, working together, we will help you
locate the problem and, chances are, correct it yourself...over the telephone without a service
call.
When more extensive service is necessary, we will assist you with direct factory trained
technicians or a qualified service organization for on-the-spot repair. If your service need is
covered by the warranty, we will arrange for the unit to be repaired at our expense and to your
satisfaction.
Regardless of your needs, our professional telephone technicians are available to assist you
Monday through Friday from 8:00 a.m. to 6:00 p.m. Eastern Time. Please contact us by
telephone or fax. If you wish to write, our mailing address is:
Thermo Fisher Scientific (Asheville) LLC
401 Millcreek Road, Box 649
Marietta, OH 45750
International customers, please contact your local Thermo Scientific distributor.
iv 1300 Series A2
Preface

1300 Series A2 vThermo Scientific
Table of Contents
Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Safety Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2
HEPA Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Paper atch Grids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
ontrol Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5
Use of the Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
Unit Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-8
hamber Lighting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-9
UV Lights . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-10
Work Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-10
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Optional Exhaust Transition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2
Exhaust System Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2
Moving the Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-4
Service Valve onnections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-5
Universal Piping onnections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-7
SmartPort . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-8
Drain Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-8
Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Assemble the Stand . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Unlock ounterweight . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-3
Level the abinet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-3
Power onnection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-4
Installation Tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-5
Locating a ertifier . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-6
peration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Unit Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3
Operating Modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3
Loading the hamber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-4
Error odes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-5
Working Recommendations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-5
Unit Shut-down . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-7
Extended Periond Shut-down . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-7
Unit Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-7
Section 1
Section 2
Section 3
Section 4

vi 1300 Series A2 Thermo Scientific
Table of Contents
Cleaning / Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
are and leaning of Stainless Steel . . . . . . . . . . . . . . . . . . . . . . . . .5-1
leaning and aring for oated Surfaces . . . . . . . . . . . . . . . . . . . . .5-2
Disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
Rinsing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
UV Disinfection After leaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
hange the UV Disinfection Time . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Microbiological Space Decontamination . . . . . . . . . . . . . . . . . . . . . .5-4
lean Exterior Surfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-5
lean Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-5
lean the Drain Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-5
lean the Paper atch Grid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-6
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2
Sample hamber Lights . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2
Optional UV Lights . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2
Replacements and Repairs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-3
Unit Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-3
Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1
Certification Testing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1
lassification of the Safety abinet . . . . . . . . . . . . . . . . . . . . . . . . . .8-1
Test Terms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1
Testing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-2
Test Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-3
Testing Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-3
HEPA Filter Leak Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-10
Filters that can be Accessed and Scanned . . . . . . . . . . . . . . . . . . .8-10
Filters that cannot be Accessed or Scanned . . . . . . . . . . . . . . . . . .8-11
Airflow Pattern Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-11
Elect. Leakage, Ground Resistance, Polarity Tests . . . . . . . . . . . . .8-12
Site Installation Assesment Tests . . . . . . . . . . . . . . . . . . . . . . . . . .8-12
Data Log/Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1
Section 5
Section 6
Section 8
Section 7
Section 9

1300 Series A2 1-1Thermo Scientific
Section 1 Description
The Thermo Scientific 1300 Series A2 offers innovative SmartFlow™
technology; an automatic airflow compensation system that adjusts motor
speed as filters load, without the use of a manual damper. The
SmartFlow™ system ensures safe working conditions, even between annual
certifications.
The plenum assembly consists of a plenum for the downflow blower and a
plenum for the exhaust air blower. Each blower includes a filter. The
exhaust air is discharged through an opening in the top of the cabinet.
hamber lighting includes one fluorescent bulb for 4 foot models and two
for 3, 5 & 6 foot models.
The optional, factory-installed UV light is installed in the ceiling at the
front of the sample chamber.
Figure 1-1. Unit Components
WRSDFFHVVSRLQWVULJKWVLGHDFFHVVSRLQWVERWWRPRIFDELQHWDFFHVVSRLQWVERWWRPRIFDELQHWDFFHVVSRLQWVOHIWVLGHDFFHVVSRLQWV
FXIWXQLWVRQO\GUDLQYDOYH

1-2 1300 Series A2 Thermo Scientific
Section 1
Description
Safety Systems
Three service valve access ports are located on each side panel. When
installing service valves, remove the center of the port plugs at the
perforations.
The optional stand may be ordered in two configurations; a manually
adjustable from 30” to 38” working height, and a fixed 34” working
height with attached casters.
A stainless steel one-piece work surface is standard. Optional armrests
ensure a comfortable working position for the user and minimize
blockage of airflow at the front grille.
Internal outlets, provided as the power source for accessories, are provided
standard in the following configuration:
1305, 1307, 1320, 1321, 1322, 1323, 1325, 1327, 1335, 1337, 1339,
1340, 1341, 1342, 1345, 1347, 1350, 1351, 1352, 1353, 1355, 1357,
1368, 1369, 1370, 1371, 1375, 1377, 1385, 1387, 1395, 1397: two
duplex right and left side
1306, 1308, 1326, 1328, 1330, 1331, 1332, 1333, 1336, 1338, 1346,
1348, 1358, 1359, 1366, 1367, 1372, 1373, 1376, 1378, 1380, 1381,
1382, 1383, 1384, 1388, 1389, 1390, 1391, 1392, 1393, 1396, 1398:
two single right and left side
Test hoses (aerosol challenge ports) are located under the work tray. The
supply plenum hose is located on the left and the exhaust plenum hose on
the right.
Note Do not remove the caps of the two test hoses to check for supply
and exhaust airflow. s
Negative pressure air system
A negative pressure air system combines with HEPA filters in the
supply and exhaust airflow for personnel and product protection.
Personnel protection
Inflow air along the entire working opening at a constant high velocity
prevents leakage from the work opening of the chamber.
Exterior air pressure being higher than the internal air pressure creates
negative pressure, ensuring containment in case of cabinet leakage.
Product protection
Steady airflow within the air system ensures constant downflow,
allowing the HEPA filters to remove contaminants so that the samples
are always surrounded by ultra-pure air. Harmful particles are not
carried over the sample chamber (protection from cross-contamination).

1300 Series A2 1-3Thermo Scientific
Section 1
Description
HEPA filters
The downflow air within the chamber and the exhaust air are cleaned by
HEPA filters.
Safety lockout
To protect from UV radiation, the optional UV light will not turn on if
the front window is open. While the UV light is on, the front window
should remain closed. The closed window blocks out the UV rays.
Opening the window will cause the UV light to turn off.
Digital Airflow Verification (DAVe)
Independent monitoring of inflow and downflow air velocities
guarantees that product and personnel protection remain
uncompromised. Airflow monitoring determines the velocity of the
airflow in the sample chamber as well as the inflow velocity of air
through the exhaust opening. As soon as airflow velocities rise above or
fall below a specified safety value, an audible and a visual alarm is
activated.
Window position monitoring
The position sensors detect the size of the front window opening and
indicate whether the window is open to the specified work position,
closed (energy saving) or in an unsafe intermediate position.
SmartFlow Indicator
The SmartFlow Indicator provides assurance that the inflow is balanced
properly. SmartFlow monitors the advanced motor technology adjusting
airflows even as the filter resistances change due to loading or other
influences. The SmartFlow Indicator visually demonstrates the cabinet’s
ability to compensate to maintain personnel protection. Green indicators
show the fan and filters have power and capacity in reserve to respond to
additional demands. Yellow or red indicators show that while the cabinet
is providing personnel and product protection, maintenance should be
scheduled to restore the compensation reserve.
Safety Systems
(continued)