Thermo Scientific Cell Locker User manual

Cell Locker 50155007_C 27.05.21 Thermo Fisher Scientific
Cell Locker
50155007 Revision C May 2021
Operating Instructions

Cell Locker 50155007_C 27.05.21 Thermo Fisher Scientific
Preface
Preface
© 2021 Thermo Fisher Scientific Inc. All rights reserved.
Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to
use in the product operation. This document is copyright protected and any reproduction of the
whole or any part of this document is strictly prohibited, except with the written authorization of
Thermo Fisher Scientific Inc.
The contents of this document are subject to change without notice.
Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate
or error-free and assumes no responsibility and will not be liable for any errors, omissions, damage
or loss that might result from any use of this document, even if the information in the document is
followed properly.
This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a
purchaser. This document shall in no way govern or modify any Terms and Conditions.
In case of conflicting translations into foreign languages the German-language version of these
operating instructions shall be binding.
Trademarks
CELL LOCKER® is a registered trademark of Thermo Scientific.
Thermo Scientific is a brand owned by Thermo Fisher Scientific, Inc.
All other trademarks mentioned in the operating instructions are the exclusive property of the
respective manufacturers.
Thermo Electron LED GmbH
Robert-Bosch-Straße 1
D - 63505 Langenselbold
Deutschland
Die Thermo Electron LED GmbH is an affiliate to:
Thermo Fisher Scientific Inc.
168 Third Avenue
Waltham, MA 02451
USA

Cell Locker 50155007_C 27.05.21 1Thermo Fisher Scientific
Table of Contents
Table of Contents
Chapter 1 General notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
1.1. Explanation of safety information and symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
1.2. Safety notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
1.3. Operational Safety Rules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3
1.4. Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
1.5. Intended Purpose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
1.5.1. Correct Use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
1.5.2. Incorrect Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Chapter 2 Delivery of the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
2.1. Scope of delivery. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
2.2. Acceptance inspection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5
Chapter 3 Description of the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
3.1. Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
3.2. First use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
3.3. Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
3.4. Application versions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Chapter 4 Cleaning and disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
4.1. Cleaning. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
4.2. Wipe/spray disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
4.3. Autoclaving. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Chapter 5 Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
5.1. Membrane filter replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
5.2. Cover gasket replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Chapter 6 Spare and wear parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Chapter 7 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Chapter 8 Technical Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

Cell Locker 50155007_C 27.05.21 2Thermo Fisher Scientific
Chapter 1 General notes
1. General notes
1.1 Explanation of safety information and symbols
Warning
Indicates a hazardous situation which, if not avoided, could result in death or serious injuries.
Caution
Indicates a hazardous situation which, if not avoided, could result in minor or moderate injuries.
Notice
Indicates a situation which, if not avoided, could result in damage to equipment or property.
In chapters of the instruction manual which deal with safety, this icon appears under the title of the
chapter. Displayed on the equipment, this icon denotes that special attention must be paid to the
information given in the instruction manual or accompanying documents.
Is used for application hints and useful information
Harmful liquids!
Fire hazard!
Toxic substances!
Wear safety gloves!
Wear safety goggles!
Wear breathing protection!

Cell Locker 50155007_C 27.05.21 3Thermo Fisher Scientific
Chapter 1 General notes
1.2 Safety notes
The CELL LOCKER may only be used by instructed personnel.
The present operating instructions, applicable safety data sheets, hygiene guidelines and the
corresponding technical rules issued by the operator shall be used to create written procedures
targeted at personnel working with the subject matter device, detailing:
• the decontamination measures to be employed for the CELL LOCKER and the accessories used with
it,
• the safety precautions to be taken when processing specific agents,
• wearing protective equipment when handling e.g. microbiological and biological samples,
• the measures to be taken in case of accidents,
• drying time after decommissioning, as Transport 2 hours,
• working according to GLP - Good Laboratory Practice.
1.3 Operational Safety Rules
Observe the sample weight limits as a whole and its shelving in particular.
Check the door seal every six months for proper sealing performance and possible damage.
Do not process any samples containing hazardous chemical substances that may be released into
the ambient air through defective seals or may cause corrosion or other defects.
1.4 Warranty
Thermo Fisher Scientific warrants the operational safety and functions of the CELL LOCKER for one
year only under the condition that:
•theCELL LOCKER is operated and serviced exclusively in accordance with its intended purpose and
as described in these operating instructions,
• the device is not modified,
• only original spare parts and accessories that have been approved by Thermo Fisher Scientific
are used (third-party spares without Thermo Fisher Scientific approval void the limited warranty),
• inspections and maintenance are performed at the specified intervals,
• an operation verification test is performed after each repair activity.
The warranty is valid from the date of delivery of the CELL LOCKER to the customer.

Cell Locker 50155007_C 27.05.21 4Thermo Fisher Scientific
Chapter 1 General notes
1.5 Intended Purpose
1.5.1. Correct Use
The CELL LOCKER is intended for use in laboratory equipment, which among others is used for the
preparation and cultivation of cell and tissue cultures for research applications.
The maximum Autoclave temperature is 15 minutes at 121°C (250 °F) with fast exhaust.
1.5.2. Incorrect Use
Do not use tissues, substances or liquids that:
• are easily flammable or explosive;
• release vapor or dust that forms combustible or explosive mixtures when exposed to air;
• release poisons;
• release dust;
• exhibit exothermic reactions;
• are pyrotechnical substances.
Do not pour any liquids on the bottom of the interior surface or into a collecting basin
inside the unit.
The CELL LOCKER is not for use in Medical Devices.

Cell Locker 50155007_C 27.05.21 5Thermo Fisher Scientific
Chapter 2 Delivery of the device
2. Delivery of the device
2.1 Scope of delivery
2.2 Acceptance inspection
After the CELL LOCKER has been delivered, check the delivery immediately for:
• completeness,
• possible damage.
If components are missing or damage is found on the device or the packaging, please contact the
carrier, as well as the customer service, immediately.
Description Pcs.
CELL LOCKER 1
Membrane filter 2
Work tray with cover 1
Consumables kit 1
Operating instructions 1
Summarized safety instructions 1

Cell Locker 50155007_C 27.05.21 6Thermo Fisher Scientific
Chapter 3 Description of the device
3. Description of the device
3.1 Description
The CELL LOCKER consists of a polycarbonate chamber with two membrane filters, which are
permeable to water vapor and gas, but are impermeable to microorganisms.
Fig. 1. CELL LOCKER
Fig. 2. Bottom marks
Position Name
1 work plate
2 membrane frame with filter
3 gasket
4 latch
5cover
6handleknob
1
2
3
4
5
6
Production
year
Production
month
Polycarbonate
Makrolon 2528

Cell Locker 50155007_C 27.05.21 7Thermo Fisher Scientific
Chapter 3 Description of the device
3.2 First use
When using the first time, clean and disinfect the CELL LOCKER.
3.3 Operation
The CELL LOCKER can be placed into the incubator with 6-door gas tight screen with or without cover.
When inserting into a laboratory device make sure that the CELL LOCKER is not twisted.
3.4 Application versions
Depending on user preference, the CELL LOCKER can be modified to attach or remove the transport
cover, using the consumables kit:
Fig. 3. Consumables kit
Note
Clean the CELL LOCKER before inserting the membrane frame with filter.
Warning
The CELL LOCKER must be removed before the incubator is sterilized.
4 x round plug
2 x rectangular plug
2 x screw M4
1 x Allen key

Cell Locker 50155007_C 27.05.21 8Thermo Fisher Scientific
Chapter 3 Description of the device
Cover can be attached to work tray with the Allen key, allowing protected transport.
Fig. 4. CELL LOCKER options
with attached transport cover without transport cover
without cover to remove cultures with single
door access
with cover to remove CELL LOCKER with cultures
samples access inside the chamber with
transport cover
Note:
Attach cover for protected transport
to biosafety cabinet or to another
incubator or lab. Use of cover in the
chamber may require two hand
opening.