Thermo Scientific Medifuge User manual

Thermo Scientific Medifuge
Small Benchtop Centrifuge
Instructions for Use
50148677_f • 08 / 2017
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Table of Contents
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Intended Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Items Supplied . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Signal Words and Colors� � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 7
Set Up Conditions� � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 7
Shutdown � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 7
Preparation � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 8
Hazardous Substances � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 8
Operation� � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 9
Maintenance � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 9
Service Lifetime � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 10
Symbols used on the Centrifuge . . . . . . . . . . . . . . . . . . . . . . 11
Symbols used in the Manual . . . . . . . . . . . . . . . . . . . . . . . . . 11
I. Technical Specifications . . . . . . . . . . . . . . . . . . . . . . . . . 12
1. Technical Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Thermo Scientific Medifuge Centrifuge � � � � � � � � � � � � � � � � � � � � � � � 12
Thermo Scientific DualSpin Rotor � � � � � � � � � � � � � � � � � � � � � � � � � � � 13
2. Rotor and Accessories. . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
3. Directives, Standards and Guidelines. . . . . . . . . . . . . . . . 15
4. Mains Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
II. Transport and Set Up . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
1. Before Setting Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
2. Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
3. Transporting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
4. Leveling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
5. Mains Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

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6. Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
7. Shipping . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
III. Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Control Panel Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Select RPM/RCF� � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 22
Select Runtime� � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 24
Acceleration / Deceleration Profiles� � � � � � � � � � � � � � � � � � � � � � � � � � 25
Programs� � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 26
Acoustic Signals� � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 27
IV. Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
1. Switching on the Centrifuge . . . . . . . . . . . . . . . . . . . . . . . 28
2. Open the Centrifuge Door . . . . . . . . . . . . . . . . . . . . . . . . . 28
3. Rotor Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Installation of Swing Out Buckets � � � � � � � � � � � � � � � � � � � � � � � � � � � 31
4. Rotor Temperature Range . . . . . . . . . . . . . . . . . . . . . . . . . 32
5. Rotor Loading. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Maximum Loading � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 34
Tube and Spacer Guide � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 35
Swing Out Bucket � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 39
Checking the Rotor Lifetime � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 41
6. Close the Centrifuge Door . . . . . . . . . . . . . . . . . . . . . . . . . 42
7. Centrifugation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Before a Run � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � �43
Starting the Centrifugation � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � �43
Stopping the Centrifugation � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 44
8. Removing the Rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
9. Switch off the Centrifuge. . . . . . . . . . . . . . . . . . . . . . . . . . 45
V. Maintenance and Care . . . . . . . . . . . . . . . . . . . . . . . . . . 46
1. Cleaning Intervals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

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2. Basics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
3. Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
4. Disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
5. Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
6. Service of Thermo Fisher Scientific . . . . . . . . . . . . . . . . . 51
7. Shipping and Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
VI. Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
1. Mechanical Emergency Door Release. . . . . . . . . . . . . . . . 52
2. Troubleshooting by Guide . . . . . . . . . . . . . . . . . . . . . . . . . 53
3. When to contact Customer Service. . . . . . . . . . . . . . . . . . 54
Chemical Compatibility Chart . . . . . . . . . . . . . . . . . . . . . . 55
Declaration of Decontamination . . . . . . . . . . . . . . . . . . . . 68
Acceleration/Deceleration Profiles . . . . . . . . . . . . . . . . . . 70
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

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Preface
Before using the centrifuge, read through this instruction manual carefully and follow the instructions�
Not following the instructions and safety information in this instruction manual will result in the
expiration of the sellers warranty�
Intended Use
This centrifuge is intended to be used as a laboratory equipment to separate sample mixtures of
different densities�
This centrifuge can be used as an In-Vitro diagnostics device (Directive 98/79/EC), if used together
with IVD tubes, to separate blood into its components such as serum and plasma for further clinical
diagnostic analysis�
The centrifuge has to be operated by a trained individual such as a clinical laboratory technologist
or a trained laboratory technician�

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Items Supplied
The Thermo Scientific™Medifuge™small benchtop centrifuge is supplied with a rotor and 2 bucket
sets�
Size and relation of graphics are not showing real dimensions and are just for visual identification�
If any parts are missing, please contact the nearest Thermo Fisher Scientific representative�
Article No. Item Graphic Quantity
Centrifuge
Thermo Scientific Medifuge small benchtop
centrifuge
1
Power supply cable 1
Rotor
75008810 Thermo Scientific™DualSpin™rotor (factory
installed) with fixed angle and swinging
buckets sets, including:
1
Fixed angle buckets 8
Swing out buckets 8
50148478 Rotor locking nut (factory installed) 1
75008817 Spacers (short & green) 8
75008818 Spacers (long & yellow) 8
50149182 Hex key (tool for emergency lid lock) 1
Instruction manual 1
CD 1

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Precautions
Signal Words and Colors
WARNING Indicates a hazardous situation that, if not avoided, could result in
death or serious injury.
CAUTION Inidicates a hazardous situation that, if not avoided, could result in
minor or moderate injury (e.g. sample loss).
NOTICE Indicates information considered important, but not hazard-related
(e.g. messages relating to property damage).
NOTICE
Observe the safety instructions� Not following these instructions can cause damage�
The centrifuge is to be used for its intended use only� Improper use can cause damages,
contamination, and injuries with fatal consequences�
Set Up Conditions
Set up in a well-ventilated environment, on a horizontally levelled and rigid surface with
adequate load-bearing capacity�
As safety zone maintain a clear radius of at least 30 cm around the centrifuge�
Do not place any dangerous substances within this security zone�
Plug the centrifuge only into sockets which have been properly grounded�
The mains plug must be freely accessible at any time�
CAUTION
Not following the set up conditions can result in minor or serious injuries�
Shutdown
Turn off the centrifuge at the main switch�
The mains plug must be freely accessible at any time�
Press the STOP key to shut down the centrifuge�
Pull out the power supply plug or disconnect the power supply in an emergency�

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Preparation
Use only with a rotor that has been properly installed� Follow the instructions in section
“Rotor Installation” on page 29�
Do not use a rotor or accessories that show any signs of corrosion or cracks�
Contact customer service for further advice or inspections�
Use only with a rotor that has been properly loaded�
Never overload the rotor�
Always balance the samples�
Use only rotors and accessories for this centrifuge that have been approved by Thermo
Fisher Scientific�
Make sure the rotor is locked properly into place before operating the centrifuge�
WARNING
It is the obligation of the operator to make sure, that protective clothing is used� Mind the “Laboratory
Biosafety Manual” of the World Health Organization (WHO) and the regulations in your country�
CAUTION
Implement measures which ensure that no one can approach the centrifuge for longer than
absolutely necessary while it is running�
Do not use a damaged rotor� Replace the rotor, if it was dropped�
Hazardous Substances
WARNING
Especially when working with corrosive samples (salt solutions, acids, bases), the accessory
parts and vessel have to be cleaned thoroughly�
Do not centrifuge explosive or flammable materials or substances�
The centrifuge is not inert or protected against explosion� Never use the centrifuge in an
explosion-prone environment�
Do not centrifuge toxic or radioactive materials or any pathogenic micro-organisms without
suitable safety precautions�
If any hazardous materials are centrifugated, mind the “Laboratory Biosafety Manual” of the
World Health Organization (WHO) and any local regulations� When centrifuging microbiological
samples from the Risk Group II (according to the “Laboratory Biosafety Manual” of the World Health
Organization (WHO)), aerosol-tight biological seals have to be used� Look on the internet page of the
World Health Organization (www�who�int) for the “Laboratory Biosafety Manual”� For materials in a
higher risk group, extra safety measures have to be taken�

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If toxins or pathogenic substances have contaminated the centrifuge or its parts, appropriate
disinfection measures have to be taken (“Disinfection” on page 49)�
Extreme care should be taken with highly corrosive substances which can cause damage
and impair the mechanical stability of the rotor� These should only be centrifuged in fully
sealed tubes�
If a hazardous situation occurs, turn off the power supply to the centrifuge and
leave the area immediately.
Operation
WARNING
Never use the centrifuge if parts of its cover panels are damaged or missing�
Do not move the centrifuge while it is running�
Do not place anything on top of the centrifuge during a run�
CAUTION
Do not lean on the centrifuge�
Never open the centrifuge door until the rotor has come to a complete stop and this has
been confirmed in the display�
The emergency door release may be used in emergencies only to recover the samples from
the centrifuge, e�g� during a power failure (“Mechanical Emergency Door Release” on page
52)�
NOTICE
In any case of severe mechanical failure, such as a rotor crash, the centrifuge is not aerosol-tight�
In case of rotor failure the centrifuge can be damaged� Leave the room� Inform customer service�
Maintenance
WARNING
The centrifuge housing is not to be opened by the operator�
Do not change or replace mechanical or electrical components� Changing or replacing
components can result in death or serious injury�

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Service Lifetime
The centrifuge is designed for 10 years or 140 000 cycles of service, whichever is first� Usage
beyond these limits might affect the safety of the centrifuge housing or the lid latch system�
The rotor is designed for 5 years or 60 000 cycles of service, whichever is first� Usage beyond these
limits might lead to rotor failure, sample loss and damage to the centrifuge�

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Symbols used on the Centrifuge
This symbol refers to general hazards�
This symbol refers to biological hazards�
Observe the information contained in the instruction manual to keep
yourself and your environment safe�
This symbol refers to physical hazards from hot surfaces�
This symbol refers to information on hazards, described within the manual�
This symbol demands to disconnect mains before transporting or servicing
the centrifuge�
This symbol demands to make sure that a swing out bucket is installed
correctly with its fin�
Symbols used in the Manual
This symbol refers to general hazards�
This symbol refers to biological hazards�
Observe the information contained in the instruction manual to keep
yourself and your environment safe�

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I. Technical Specifications
1. Technical Data
Thermo Scientific Medifuge Centrifuge
Environmental Conditions For interior use
Altitudes of up to 3 000 m above sea level
Max� relative humidity 80% up to 31 °C;
decreasing linearly to 50% relative humidity at 40 °C
Environmental Conditions during
Storage and Shipping
Temperature: -10 °C to +55 °C
Humidity: 15% to 85%
Permissible Ambient Temperature
during Operation
+2 °C to +40 °C
Heat Dissipation 0�123 kWh; 419�7 Btu/h; 442�8 kJ/h
Overvoltage Category II
Pollution Degree 2
IP 20
Running Time 99 min; hold
Maximum Speed nmax 4 900 rpm
Minimum Speed nmin 300 rpm
Maximum RCF Value at nmax
Fixed Angle Setup
Swing Out Setup
3 114 x g
3 490 x g
Noise Level at Maximum Speed1< 56 dB (A)
Maximum Kinetic Energy 680 J
Dimensions
Height (open door / closed door)
Width
Depth
510 mm / 240 mm
325 mm
450 mm
Weight215�5 kg
1Front Side Measurement, 1 m in front of the instrument at 1.6 m height.
2Without Rotor.

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Thermo Scientific DualSpin Rotor
Maximum Permissible Load 8 x 30 g
Maximum Allowed Imbalance 10 g
Maximum Speed nmax 4 900 rpm
Maximum RCF-Value at nmax
Fixed Angle Setup 3 114 x g
Swing Out Setup 3 490 x g
Max� Cycle Number 60 000
Radius max� / min�
Fixed Angle Setup 116 mm / 37 mm
Swing Out Setup 130 mm / 42 mm
Angle
Fixed Angle Setup 45°
Swing Out Setup 12-87°
Acceleration / Braking Time*
Fixed Angle Setup 24 s / 37 s
Swing Out Setup 24 s / 31 s
Autoclavable No
*Deceleration time at standard curve.

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2. Rotor and Accessories
Article No. Description Graphic
75008810 Thermo Scientific DualSpin rotor
(1x) with fixed angle buckets (8x)
and swinging buckets (8x)
75008813 Thermo Scientific DualSpin rotor
body (1x)
75008815 Fixed angle buckets (8x)
75008816 Swing out buckets (8x)
50148478 Rotor locking nut
75008817 Spacers (green, 8x)
75008818 Spacers (yellow, 8x)
50149182 Hex key (tool for emergency lid lock)

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3. Directives, Standards and Guidelines
Region Directive Standard
Europe
220–230 V,
50/60 Hz
98/79/EC in vitro diagnostic
medical devices (IVD)
2014/35/EC Low Voltage
(protective goals)
2006/42/EC Machinery
(protective goals)
2014/30/EC Electromagnetic
Compatibility (EMC)
(protective goals)
2011/65/EC RoHS – Directive on
the Restriction of the use of certain
Hazardous Substances in electrical
and electronic equipment
EN 61010-1, 3rd Edition
IEC 61010-2-020, 3rd Edition
IEC 61010-2-101, 3rd Edition
EN 61326-1 Class B
EN 61326-2-6
EN 62304
EN 62366
EN ISO 14971
EN ISO 13485
North America
(USA & Canada)
230 V, 60 Hz
120 V, 60 Hz
FDA
Product Code JQC
Device Class 1
Centrifuges for Clinical Use
EN 61010-1, 3rd Edition
IEC 61010-2-020, 3rd Edition
IEC 61010-2-101, 3rd Edition
EN 61326-1 Class B
EN 61326-2-6
EN 62304
EN 62366
EN ISO 14971
EN ISO 13485
Japan
100 V, 50/60 Hz
China
220 V, 50 Hz
EN 61010-1, 3rd Edition
IEC 61010-2-020, 3rd Edition
IEC 61010-2-101, 3rd Edition
EN 61326-1 Class B
EN 61326-2-6
EN 62304
EN 62366
EN ISO 14971
EN ISO 13485

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4. Mains Supply
The following table contains an overview of the electrical connection data� This data is to be taken
into consideration, when selecting the mains connection socket�
Unit Thermo Scientific Medifuge small benchtop centrifuge
Article No. 75008802 75008801 75008800
Mains Voltage 100 V 120 V 220-230 V
Frequency 50/60 Hz 60 Hz 50/60 Hz
Rated Current 1�7 A 1�8 A 1�1 A
Power
Consumption 100 W 130 W 130 W
Equipment Fuse 6�3 AT 4 AT 2 AT
Building Fuse 16 AT 16 AT 16 AT

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II. Transport and Set Up
1. Before Setting Up
1� Check the centrifuge and the packaging for any shipping damage� Inform the shipping
company and Thermo Fisher Scientific immediately if any damage is discovered�
2� Remove the packaging�
NOTICE Dispose of the packaging. Do not reuse it.
3� Check, if the items supplied are complete (“Items Supplied” on page 6)�
The rotor is factory installed and tightened with the rotor locking nut inside the centrifuge� You
can only check the rotor and the rotor locking nut by opening the centrifuge door when the
centrifuge is connected to the power supply (“Mains Supply” on page 16) and switched on
(“Open the Centrifuge Door” on page 28)�
If the items supplied are incomplete, please contact Thermo Fisher Scientific�
2. Location
CAUTION UV rays reduce the stability of plastics.
Do not subject the centrifuge, rotor and plastic accessories to
direct sunlight.
The centrifuge is only to be operated indoors�
The set-up location must fulfill the following requirements:
A safety zone of at least 30 cm must be maintained around the centrifuge� People and
hazardous substances must be kept out of this safety zone while centrifuging�
The supporting structure must be stable and free of resonance�
The supporting structure must be suitable for horizontal setup of the centrifuge�
The centrifuge is not to be exposed to heat and strong sunlight�
The set-up location must be well-ventilated at all times�

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3. Transporting
WARNING The centrifuge can be damaged by impact.
Do not operate the centrifuge if an impact occured.
CAUTION Do not use a damaged rotor.
Replace the rotor, if it was dropped.
Using a damaged rotor can cause a crash.
NOTICE Always remove buckets before transporting the centrifuge.
Buckets can fall into the rotor chamber.
Always make sure that the buckets are in correct position before
operation.
Transport the centrifuge upright and with the centrifuge door closed�
4. Leveling
The centrifuge is to be placed on horizontal and level supporting structures or benching� If necessary
level the supporting structures or benching to level the centrifuge�
Horizontal level has to be checked after moving the centrifuge to a new location�

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5. Mains Connection
CAUTION Plug the centrifuge into grounded electrical sockets only.
1� Turn off the power supply switch located on the back side�
2� Check whether the cable complies with the safety standards of your country�
3� Make sure that the voltage and frequency correspond to the figures on the rating plate�
4� Establish the connection to the power supply with the connecting cable�
6. Storage
WARNING When removing the centrifuge and accessories from use, clean and
additionaly disinfect or decontaminate the entire system if biological
or chemical substances were used. If in doubt contact the Thermo
Fisher Scientific customer service.
Before storing the centrifuge and the accessories it must be cleaned and if necessary
disinfected and decontaminated�
Centrifuge, rotor, buckets and accessories have to be thoroughly dried before storage�
Store the centrifuge in a clean, dust-free location�
Be sure to place the centrifuge on its feet�
Avoid storing the centrifuge in direct sunlight�

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7. Shipping
WARNING Before shipping the centrifuge and accessories you have to clean
and additionaly disinfect or decontaminate the entire system if
biological or chemical substances were used. In doubt contact the
Thermo Fisher Scientific customer service.
Before shipping the centrifuge please keep the following in mind:
The centrifuge must be cleaned and decontaminated�
The decontamination must be confirmed with a decontamination certificate (“Declaration of
Decontamination” on page 68)�