Thermo Scientific Shandon TissueWave 2 Manual

1
TissueWave 2 Operator Guide
Shandon TissueWave 2
Microwave Specimen
Processing System
Operator Guide - Issue 1
B35210021 English

2 TissueWave 2 Operator Guide
© 2007 Thermo Fisher Scientific. All rights reserved.
Thermo Shandon Limited is an ISO 9001 and TickIT Accredited Company
Thermo Fisher Scientific is the trading name of Thermo Shandon Limited
All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries
Thermo Fisher Scientific makes every endeavour to ensure that the information contained in its
support documentation is correct and clearly stated but does not accept responsibility for any errors
or omissions. The development of Thermo Fisher products and services is continuous. Make sure
that any published information that you use for reference is up to date and relates to the status of the
product. If necessary, check with Thermo Fisher or your local Thermo Fisher representative. ▲
This manual may not, in whole or in part, be copied, photocopied, reproduced, translated, or
converted to any electronic or machine readable form without prior written consent of Thermo Fisher.
All information contained in this manual is proprietary and confidential, and the exclusive
property of Thermo Fisher Scientific. This manual is protected by copyright and any reproduction
is prohibited. This manual is for use only by the individuals to whom it has been made available
by Thermo Fisher Scientific.
Contact address
Anatomical Pathology
4481 Campus Drive
Kalamazoo
MI 49008
USA
Tel: [phone]
Fax: [phone]
www.thermo.com/shandon
The Shandon TissueWave 2 meets the following CE Mark requirements:
In Vitro Diagnostic Directive 98/79/EC
Low Voltage Directive 2006/95/EC.
®
CUS

3
TissueWave 2 Operator Guide
The following symbols and conventions are used throughout this
manual and on the instrument.
THIS SYMBOL IS USED ON THE EQUIPMENT, OR IN A DOCUMENT,
TO WARN THAT INSTRUCTIONS MUST BE FOLLOWED FOR SAFE
AND CORRECT OPERATION. IF THIS SYMBOL APPEARS ON THE
INSTRUMENT, ALWAYS REFER TO THIS OPERATOR GUIDE.
THIS SYMBOL IS USED ON THE EQUIPMENT, OR IN A DOCUMENT, TO
WARN THAT THERE MAY BE A BIOHAZARD ASSOCIATED WITH THE
INSTRUMENT. ALWAYS ACT WITH COMMON SENSE AND BE AWARE
OF THE SAMPLES USED. TAKE SUITABLE PRECAUTIONS.
THIS SYMBOL WARNS YOU THAT SURFACES ARE HOT. IF THIS
SYMBOL APPEARS ON THE INSTRUMENT, ALWAYS REFER TO THIS
OPERATOR GUIDE.
THIS SYMBOL IS USED ON THE EQUIPMENT, OR IN A DOCUMENT,
TO WARN THAT HARMFUL CHEMICALS ARE USED WITH THE
INSTRUMENT. REFER TO THE MATERIAL SAFETY DATA SHEETS FOR
THE CHEMICALS USED. ALWAYS ACT WITH COMMON SENSE AND
BE AWARE OF LOCAL LABORATORY PROCEDURES. TAKE SUITABLE
PRECAUTIONS.
A warning is given in the document if there is a danger of
personal injury or damage to samples or equipment.
Note
Notes give more information about a job or instruction but do not form part of
the instructions.
SymbolsSymbols
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4 TissueWave 2 Operator Guide
Contents
How to Use This Guide
Safety Information
1 - Introducing the Shandon TissueWave 2
2 - Daily Operation
3 - Installation
4 - Programming
5 - Troubleshooting
6 - Maintenance and Cleaning
Appendix A - Approved Reagents List
Appendix B - List of Available Names
Appendix C - Graph of Power Output
Appendix D - Power Output Confidence Test
Appendix E - Accessories
Appendix F - Warranty and Declaration of Conformity
Appendix G - Technical Specifications
Index

5
TissueWave 2 Operator Guide
How to Use This Guide
The Shandon TissueWave 2 Microwave is intended for use in
pathology laboratories by operators familiar with tissue processing
and laboratory equipment.
Before operating the instrument, the section entitled Safety
Information must be read and understood.
This Operator Guide is structured to let processing of tissues begin
quickly and safely with the Shandon TissueWave 2.
Section 1 - Introducing the Shandon TissueWave 2 This chapter
identifies the components of the Shandon TissueWave 2 Microwave
and describes their functions.
Section 2 - Daily Operation This chapter explains the daily
operation procedures required to run pre-defined protocols.
Section 3 - Installation This chapter describes the initial installation
and setup of the unit.
Section 4 - Programming This chapter explains the initial
programming of the microwave, as well as how to make changes to
existing programs.
Section 5 - Troubleshooting This chapter explains the alarm
messages and gives details on how to solve common processing
problems.
Section 6 - Maintenance and Cleaning This chapter explains the
daily, weekly and monthly cleaning and maintenance requirements
for the unit.
IntroductionIntroduction
Chapter
Summary
Chapter
Summary

6 TissueWave 2 Operator Guide
Safety Information
The Shandon TissueWave 2 is designed and made with care.
The instrument is safe to use, simple to operate, and easy to maintain.
Many safety features have been built into the unit to ensure its safe
operation.
However, incorrect actions by a user may damage the equipment, or
cause a hazard to health.
All users must read and understand the following paragraphs
before using this instrument.
Operate the unit from a properly grounded and
appropriately rated receptacle.
To minimize the risk of electrical shock and the possibility
of interference with other electronic devices, the unit must
always be powered from a grounded receptacle.
Position the Shandon TissueWave 2 so that it is possible to
interrupt the mains supply at the source by removing the plug
from the socket.
In addition, the unit should be operated on its own electrical
power circuit with a current rating at least equal to the unit’s
fuse rating. Line voltage may fluctuate particularly if other
devices are powered on the same line.
The shortest possible distance between the service outlet
and the main connection feeding the service will provide
the best results in terms of consistent power output from the
microwave.
Ensure proper operation of the vent system.
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7
TissueWave 2 Operator Guide
The Shandon TissueWave 2 vent system interlock is designed
to prevent use of the unit unless adequate air flow has been
established through the microwave chamber.
Keep the exhaust hose clean and unrestricted. This provides
protection against the buildup of possibly toxic or explosive
fumes inside the unit.
Never attempt to operate the unit with a known deficiency in
the venting unit.
Do not operate the unit when empty.
Whenever operating the Shandon TissueWave 2 unit, be
sure that the microwave chamber contains some type of
microwave absorbent material. Use only microwavable
containers in the Shandon TissueWave 2.
Insufficient or improper loads or improper containers
(metal) will cause the magnetron or parts of the microwave
interior to overheat. When this happens, a thermal switch
will eventually turn the unit off so the magnetron can cool.
Several minutes will need to elapse before the switch resets
and allows the unit to operate.
Damage to the magnetron may occur with insufficient loads.
Never heat food in a laboratory microwave.
Do not put excessive weight on the door.
For example, the door is not designed to support the weight
of someone leaning on it. Applying heavy loads to the door
might cause it to become misaligned and result in improper
operation of the unit.
Do not attempt to operate the unit with something caught in
the door or with the door open.
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8 TissueWave 2 Operator Guide
There are two safety interlocks on the door to prevent open-
door operation.
Do not allow residue to build up on the door seals. Seals may
be cleaned with a mild detergent and warm water using a
soft sponge or cloth.
Do not operate the unit if the door, hinges or door seals are
damaged.
Use caution when heating volatile and / or flammable
materials.
Volatile material may evaporate quickly in the microwave
chamber and should be closely monitored during processing.
To prevent the build-up of an explosive mixture in the
chamber, flammable materials should not be heated to their
boiling point.
Reagents containing metals (eg: Zinc Formalin ot Mercuric
Chloride) need to be completely cleaned off of all internal
components. Build up of such metals may cause arcing to
occur.
Do not install the unit next to or near a source of heat.
Do not install the unit at or above eye level. Make sure the
microwave fits securely on a counter space.
To avoid toxic fumes when handling heated solutions always
work with heated solutions in a properly vented area.
Never use an additional thermometer in the unit.
Only the supplied temperature probe should be used to
monitor temperature inside of the Shandon TissueWave 2.
Use only qualified microwave service personnel for
repairs.
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9
TissueWave 2 Operator Guide
The unit should never be adjusted or repaired by anyone
except qualified microwave service personnel. The outer
case of the microwave should not be removed at any time,
except by a qualified service person.
Use caution when the vacuum chamber is in use.
The vacuum line (hose) is intended for pulling gases, not
liquids. Care should be taken to not allow liquids to be sucked
into the line.
Always release the vacuum from the chamber with the
vacuum release knob on the control panel.
Specimens must be completely covered with fluid
during all processing and staining procedures.
Processing and staining fluids will disperse the heat
generated by the microwaves in an even manner throughout
the tissue sample. This provides even processing and staining
without undue heating of artifacts.
Exposed plastic cassettes and processing racks may melt if
left uncovered with fluid.
Avoid using sponge biopsy pads as they may interfere with
the exchange of heat and reagents for the specimen sample.
Material such as metal suture staples may be processed
in the unit safely as long as they are totally submersed in
processing solution.
Never heat combustible fluids above their boiling
points.
There is a potential static discharge hazard when heating
combustibles.
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10 TissueWave 2 Operator Guide
Paraffin should be properly heated to the processing
temperature before placing the paraffin into the Shandon
TissueWave.
Never attempt to disengage the vacuum unit by
disconnecting the tubing or lid.
This will cause a backup of fluid into the tubing and possibly
the vacuum pump.
Always disengage the vacuum by releasing the vacuum knob
located on the control panel.
If this equipment is used in a manner not specified by
Thermo Fisher Scientific, the protection offered by the
equipment may be impaired.
The Shandon TissueWave 2 as supplied, complies with EN 61010-1:2001.
However, chemicals introduce potential hazards and Thermo
has adopted the following position with regard to the subject
of heated surfaces and volatile chemicals used in medical
laboratories:
i Non-specified chemicals are used in the instrument at the
customer’s own risk.
ii All the chemicals recommended by Thermo have auto-ignition
temperatures considerably above any surface temperature
that can be reached during a single fault failure of the heating
system on the instrument.
iii The instrument contains no source of ignition in any areas of
the instrument where chemicals are stored or likely to leak in a
single fault condition.
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Chemical SafetyChemical Safety

11
TissueWave 2 Operator Guide
iv The operator is fully aware of the contents of the specification
documents detailing the properties of the chemicals they are
using.
v The operator has carried out any legally required assessment
of chemicals used and is using good laboratory practice.
Some chemicals used during tissue processing are flammable
- do not use sources of ignition in the vicinity of this instrument
when it is loaded with reagents.
This product is required to comply with the European Union’s Waste
Electrical and Electronic Equipment (WEEE) Directive 2002/96/EC. It is
marked with the following symbol:
Thermo Fisher Scientific has contracted with one or more recycling/
disposal companies in each EU Member State, and this product
should be disposed of or recycled through them.
Further information on Thermo Fisher’s compliance with these
Directives, the recyclers in your country, and information on Thermo
Fisher products which may assist the detection of substances subject
to the RoHS Directive are available at:
www.thermo.com/WEEERoHS.
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EnvironmentalEnvironmental

12 TissueWave 2 Operator Guide
1 - Introducing the Shandon TissueWave 2
Welcome to the Shandon TissueWave 2 Specimen Processing
Microwave System.
This unit may be used for:
Tissue fixation and processing
Decalcification of hard material
Routine special stains where heat would accelerate the
staining process
Epitope enhancement during immunohistochemistry staining.
With a total capacity of 14 protocols consisting of a total of 63 steps,
the Shandon TissueWave 2 is designed for greater flexibility to meet
the growing laboratory workload demands.
This user friendly instrument will free-up technical time for more
complex procedures.
The Shandon TissueWave 2 will deliver uniformly excellent results in
a fraction of the time.
Laboratory safety is always an important consideration when
purchasing equipment.
The Shandon TissueWave 2 has a ventilation system interlock that
prevents the unit from operating if there is not adequate air flow
established through the microwave chamber.
The Shandon TissueWave 2 has an interactive LCD screen that
directs and informs the user throughout the operation of the unit.
A custom designed vacuum chamber is an added accessory that
comes with the Shandon TissueWave 2.
This unique chamber allows for a more rapid microwave fixation and
consistent paraffin wax infiltration.
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IntroductionIntroduction

13
TissueWave 2 Operator Guide
Identification
of Parts
Identification
of Parts
T
ISSUE
W
AVE
2
S
HANDON
-15
-20 -10
-25 -5
-30 0
SMC
inHg
T
ISSUE
W
AVE
2
S
HANDON
LCD
Display
Vacuum
Gauge
Vacuum
Control
Knob
Keypad
Power
Switch
Door
Handle
Mag
Coolin
T
ISSUE
W
AVE
2
S
HANDON
-15
-20 -10
-25 -5
-30 0
SMC
inHg
T
ISSUE
W
AVE
2
S
HANDON
LCD
Display
Vacuum
Gauge
Vacuum
Control
Knob
Keypad
Power
Switch
Door
Handle
Mag
Coolin
F1 F2
F1,F2: 15A 250V SLOW BLOW
REPLACE ONLYWITH
SAMETYPE AND RATING
EASTGRANBY,CONNECTICUT, USA
ENERGY BEAM SCIENCES INC.
V~AHz
FREQUENCY2450MHz
MANUFACTURED
MODEL
SERIAL
®
CUS
Power
Switch
Fuses
Magnatron
Cooling Outlet
Power
Socket
Serial
Port
4” Chamber
Outlet
Vacuum
Accumulator
Vacuum / Fluid
Ports
Electrical
Rating Plate
F1 F2
F1,F2: 15A 250V SLOW BLOW
REPLACE ONLYWITH
SAMETYPE AND RATING
EASTGRANBY,CONNECTICUT, USA
ENERGY BEAM SCIENCES INC.
V~AHz
FREQUENCY2450MHz
MANUFACTURED
MODEL
SERIAL
®
CUS
Power
Switch
Fuses
Magnatron
Cooling Outlet
Power
Socket
Serial
Port
4” Chamber
Outlet
Vacuum
Accumulator
Vacuum / Fluid
Ports
Electrical
Rating Plate

14 TissueWave 2 Operator Guide
Temperature
Probe
Vacuum
Tube
Exhaust
Port
Air
Agitator
Vacuum
Chamber
Agitator
Valve Temperature
Probe
Vacuum
Tube
Exhaust
Port
Air
Agitator
Vacuum
Chamber
Agitator
Valve

15
TissueWave 2 Operator Guide
The Shandon TissueWave 2 has an interactive LCD Display which
directs and informs the user throughout operation.
The Keypad is used to perform various actions and to interact with
the LCD Display.
The Keys on the Keypad have the following functions:
Run
Stop
Quit
Enter
Cursor Left / Right
Cursor Up / Down
Increase / Decrease Value
Vacuum On / Off
Keypad
Functions
Keypad
Functions
TISSUEWAVE 2
SHANDON START
SET UP
TISSUEWAVE 2
SHANDON START
SET UP

16 TissueWave 2 Operator Guide
Interior Light On / Off
Agitation On / Off
Function Keys*
* The Function Keys will perform Screen Specific Functions
which are noted on the LCD Display adjacent to the Function Keys.

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TissueWave 2 Operator Guide
In addition to those on the Keypad, there are a few other symbols
used on this instrument.
The meanings of these symbols are as follows:
Additional
Symbols Used On
the Instrument
Additional
Symbols Used On
the Instrument
Power On / Off Switch
Serial Port Connector
Vacuum Ports
Power On / Off Switch
Serial Port Connector
Vacuum Ports

18 TissueWave 2 Operator Guide
The Shandon TissueWave 2 has several features designed to
maximise the efficiency and quality of specimen processing.
These features are as follows:
Ventilation
Vacuum Accumulator
Temperature Probe
Vacuum Chamber
Air agitator
Interior Light
The Shandon TissueWave 2 has a Vent System Interlock that
prevents the unit from operating if there is an inadequate air flow
established through the microwave chamber.
This prevents the accumulation of dangerous and toxic fumes.
The vent system on the Shandon TissueWave 2 is designed to be fed
to a Powered Exhaust System.
A 4” (100 mm) Chamber Outlet is attached to the back of the unit
for easy exhaust hook-up.
An Aluminium 4” (100 mm) Diameter Hose is included with the
unit for ventilation.
Note:
It is recommended that aluminium hosing be used since plastic hoses have a
tendency to collapse at bends.
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Features of
the Shandon
TissueWave 2
Features of
the Shandon
TissueWave 2
VentilationVentilation

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TissueWave 2 Operator Guide
The Shandon TissueWave 2 Vacuum Accumulator is designed to
prevent the accidental ingestion of liquid paraffin or other
contaminants by the system’s Vacuum Pump.
Should liquids enter the Vacuum System via the Vacuum Inlet
Connection inside the Processor Cavity, they will be discharged
into the Accumulator Canister and collected at the bottom.
Paraffin will solidify and remain there until re-heated for removal.
The accumulator’s collection capacity before vacuum operation is
affected is approximately 15 fl oz (450 ml).
The flow rate of the Vacuum Pump is 2200 in³/min (0.036 m³/min).
The Flexible Temperature Probe may be used in conjunction with
the Air Agitator or threaded through the Vacuum Chamber during
Paraffin Infiltration.
Vacuum
Accumulator
Vacuum
Accumulator
Temperature
Probe
Temperature
Probe

20 TissueWave 2 Operator Guide
The probe guarantees accurate temperature measurement to ±1°C in
most aqueous solutions.
The Temperature Sensor is located at the tip of the probe, although
at least 1” (25 mm) of the probe must be covered by fluid.
When placing the Temperature Probe into a Container, the
tip should be located as closely as possible to centre of the
processing fluid within the container, and at mid-depth in the fluid.
This will produce the most reliable and stable reading of the fluid
temperature.
The Temperature Probe is made from stainless steel and is quite
flexible, allowing it to be bent and formed as required for placement
within various shaped containers.
The Vacuum Chamber is designed for use with the Shandon
TissueWave 2 during Paraffin Infiltration.
Never use vacuum when microwaving alcoholic solutions, as
this can cause rapid boiling and evaporation of the fluids.
Vacuum
Chamber
Vacuum
Chamber
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