Thermo Scientific Multidrop Pico 1 User manual

Thermo Scientific™
Multidrop™ Pico 1 Digital Dispenser
Operating manual
Cat. no. 1509060 Rev. 01

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Thermo Fisher Scientific Thermo Scientific™ Multidrop™ Pico 1 Digital Dispenser Operating manual 3
About This User Manual
The following symbols and markings appear in this user manual.
Warning / Caution Risk of injury to the user. Risk of damage to the
instrument, other equipment or loss of performance or function in a
specific application.
Note Marks a tip, important information that is useful in the optimum
operation of the system, or an item of interest.
Tip Gives a helpful hint for getting the most out of the software
functionality.
Warnings and
other markings
used in the
documentation

Thermo Fisher Scientific Thermo Scientific™ Multidrop™ Pico 1 Digital Dispenser Operating manual 5
Contents
About This User Manual............................................................................... 3
Warnings and other markings used in the documentation ................3
Introduction to the Multidrop Pico dispenser.......................................... 7
How the Multidrop Pico dispenser works........................................ 7
Advantages of the Multidrop Pico dispenser ....................................8
Safety................................................................................................................ 9
General safety rules.........................................................................9
Personal protective equipment ........................................................9
Possible inhalation hazard...............................................................9
Electrical safety ............................................................................ 10
Electrical emissions safety ............................................................. 10
Decontamination prior to servicing............................................... 10
Safe disposal of cassettes and pipette tips ....................................... 10
Ergonomic safety.......................................................................... 11
Additional assistance..................................................................... 11
System specifications................................................................................. 12
Intended use ................................................................................12
Hardware..................................................................................... 12
Environmental operating conditions ............................................. 12
Shipping and storage conditions.................................................... 12
Compatible well plates..................................................................13
Additional specifications............................................................... 13
Understanding the Multidrop Pico dispenser........................................ 14
Hardware components .................................................................14
Assembly and startup.................................................................... 15
Adjusting the cassette-to-plate height ............................................17
Running a protocol ...................................................................................... 18
Run/Simulation Window layout – plate and fluid preparation........18
Status area.................................................................................... 19
Pause/Abort ................................................................................. 19
Well plate grid display.................................................................. 20
Plate prompt and loading a plate................................................... 20
Loading a plate............................................................................. 21
Cassette prompt and loading a cassette .......................................... 21
Loading a cassette......................................................................... 22
Load fluid prompt........................................................................ 22
Modifying load volume ................................................................23
Chap
ter 1
Chapter 2
Chapter 3
Chapter 4
Chapter 5

Contents
6Thermo Scientific™ Multidrop™ Pico 1 Digital Dispenser Operating manual Thermo Fisher Scientific
Loading the fluids ........................................................................ 24
Fluid dispensing........................................................................... 26
Plate changes................................................................................ 26
Cassette changes........................................................................... 26
Preventative maintenance procedures....................................................27
Turning off the Multidrop Pico dispenser ..................................... 27
Cleaning the Multidrop Pico dispenser.......................................... 28
Pogo pin inspection...................................................................... 29
Pogo pin cleaning......................................................................... 30
A1 alignment check...................................................................... 30
Load and validate ......................................................................... 31
Fluid and Dispense....................................................................... 32
Adjust.......................................................................................... 32
A1 realignment ............................................................................ 33
Preventive maintenance completion form...................................... 34
Legal and regulatory notices .....................................................................35
Chapter 6

Thermo Fisher Scientific Thermo Scientific™ Multidrop™ Pico 1 Digital Dispenser Operating manual 7
Chapter 1
Introduction to the Multidrop Pico
dispenser
The Multidrop Pico dispenser provides fast and reliable dispensing
performance across a large dynamic range, eliminating slow, wasteful,
and error-prone manual serial dilutions and enabling assay
miniaturization through low-volume assay component addition.
The Multidrop Pico dispenser provides fast and reliable dispensing
across a wide range of life science applications:
∂Dose-response curves for small molecule and biomolecule-based
drug discovery research
∂Drug-drug synergy experiments for drug discovery
∂Low-volume assay component dispensing of a wide range of
fluids into microplates
∂Complex enzyme kinetic studies
∂DMPK standards
Using single-use cassettes to help avoid the possibility of cross-
contamination, the Multidrop Pico dispenser offers non-contact digital
dispensing in the picoliter to microliter range. It combines walk-up
convenience, ready-to-run performance and flexible, intuitive
experiment design software to increase research productivity, reliability
of results, and speed of life science research.
Even the most complex experimental plate layouts, such as drug-drug
interaction (synergy) studies, can be made simple. Titrating one drug
down the plate while another is titrated orthogonally across the plate
enables two or more drug doses to be independently generated in every
well. In addition, plate layouts can be randomized to reduce the impact
of edge effects, increasing the integrity of the experimental data. The
Multidrop Pico dispenser offers a convenient solution to address the
most challenging experiments.
The Multidrop Pico dispenser offers non-contact digital dispensing
utilizing single-use cassettes to avoid contamination in picoliter to
microliter range. It increases research productivity by reliable results
provided at higher speed using flexible and intuitive experiment design
software.
How the
Multidrop Pico
dispenser works

Introduction to the Multidrop Pico dispenser
8Thermo Scientific™ Multidrop™ Pico 1 Digital Dispenser Operating manual Thermo Fisher Scientific
The Multidrop Pico dispenser adds breakthrough capability to life
science research workflows by offering picoliter-to-microliter non-
contact dispensing directly into assay plates.
•High-Performance Digital Dispensing: Reliably dispense drop-
by-drop from low picoliters to microliters in seconds. Easily
complete set-ups even for the most complex experiments such as
drug-drug co-titration or enzyme kinetic studies in minutes.
Achieve excellent data resolution (0.02 Log Increments).
Capable of digitally randomized plate layouts to reduce edge
effects and increase data integrity.
•Resource Savings: Eliminate slow, error prone, wasteful manual
serial-dilution steps. Optimize the use of precious materials and
research time. Avoid ergonomic injuries and rework associated
with repetitive hand pipetting processes.
•Ease of Use: Enjoy a simple, intuitive user experience with
quick do-it-yourself experiment design software. Use the
Enzyme Profile Wizard to aid in designing complex enzyme
kinetic experiments Boost productivity with walk-up, just-in-
time bench top convenience. Avoid carry-over and
contamination with single use, non-contact dispensing supplies.
The Multidrop Pico dispenser is applicable to many low-
volume assay procedures with one system.
Advantages of
the
Multidrop
Pico dispenser

Thermo Fisher Scientific Thermo Scientific™ Multidrop™ Pico 1 Digital Dispenser Operating manual 9
Chapter 2
Safety
The Multidrop Pico dispenser should be used in accordance with all
applicable safety rules that are in effect in the lab in which it is installed.
Use of the Multidrop Pico dispenser should also follow common-sense
Multidrop safety guidelines for chemical, mechanical, and electrical
safety.
Note If this unit requires servicing, or you are unsure about any aspect
of operation or safety, contact the Service Help Desk. The contact
information can be found in the Multidrop Pico dispenser
Service and
Support document.
Caution Do not remove any of the Multidrop Pico dispenser covers.
Contact your Service Help Desk if the Pico dispenser is not working
properly. Do not attempt to repair the Multidrop Pico dispenser
yourself.
•Place the Multidrop Pico dispenser on a work surface that
supports all four feet securely.
•Plug the Multidrop Pico dispenser into a standard, grounded,
electrical outlet. Note the power requirement listed on the back
label of the Multidrop Pico dispenser.
•Avoid the moving parts of the Multidrop Pico dispenser during
operation.
The safety precautions and personal protective equipment (PPE)
specified by your employer for chemicals used and fluid transfer
operations and disposal should be used when operating the Multidrop
Pico dispenser and handling these materials.
The Multidrop Pico dispenser was designed with chemical safety in
mind; however, it does dispense picoliter droplets of fluid and generates
a small amount (less than 1 %) by volume, of very small aerosol
droplets that might not be trapped within the well plate that you are
dispensing into. If the fluids are known or suspected to be an inhalation
or contact hazard, use the Multidrop Pico dispenser in an enclosure,
with an exhaust, such as a lab fume hood.
General safety
rules
Personal
protective
equipment
Possible
inhalation
hazard

Safety
10 Thermo Scientific™ Multidrop™ Pico 1 Digital Dispenser Operating manual Thermo Fisher Scientific
The Multidrop Pico dispenser is TUV certified for the CE mark
signifying compliance with applicable electrical regulations in the
regions where the product is sold. Electrical specifications of the
Multidrop Pico dispenser: voltage 100-240V AC, 1 phase/3 wire, 50/60
Hertz, 1.7A full load amperage.
Caution Do not touch the static eliminator electrode probe located
under the cover guard. Even if device power is off, directly touching the
probe may result in personal injury, electrical shock, or malfunction.
The Multidrop Pico dispenser has passed an FCC/EMC test for
electrical emissions safety. This device complies with part 15 of the
FCC Rules. Operation is subject to the following two conditions:
1. This device may not cause harmful interference, and
2. This device must accept any interference received, including
interference that may cause undesired operation.
Any changes or modifications to this equipment not expressly approved
by Thermo Fisher Scientific may cause harmful interference, and it
would void your warranty.
This equipment has been tested and found to comply with the limits
for a Class A digital device, pursuant to part 15 of the FCC Rules.
These limits are designed to provide reasonable protection against
harmful interference when the equipment is operated in a commercial
environment. This equipment generates, uses, and can radiate radio
frequency energy and, if not installed and used in accordance with the
instruction manual, may cause harmful interference to radio
communications. Operation of this equipment in a residential area is
likely to cause harmful interference in which case users will be required
to correct the interference at their own expense. This Class A digital
apparatus also complies with Canadian ICES -003.
Service personnel may require you to certify that appropriate cleaning,
decontamination, and other abatement of potential threats to service
personnel have been performed prior to shipping the Multidrop Pico
dispenser for servicing. Recommended cleaning and decontamination
procedures are described in the Cleaning the Multidrop Pico dispenser
section.
Disposal of used pipette tips and cassettes should be performed in
accordance with your company’s chemical waste procedures. The
DHCs do not require any special disposal procedures except for those
required for the chemicals, which have been placed in them, by your
laboratory.
Electrical safety
Electrical
emissions safety
Decontamination
prior to servicing
Safe disposal of
cassettes
and
pipette tips