Thermo Scientific PikoReal User manual

Thermo Scientific PikoReal
Real-Time PCR System
User Manual
Rev. 2.0

Thermo Scientific PikoReal
Real-Time PCR System
User Manual
Rev.2.0, Cat. no. N11471

Copyright
Copyright ©2011 Thermo Fisher Scientific Corporation. All rights reserved. Reproduction of the
accompanying user documentation in whole or in part is prohibited.
Licensing information
Not available in Canada, Brazil, UK, Germany, Austria, Switzerland, Italy, Spain, France, Belgium, the
Netherlands, Liechtenstein, Denmark and Sweden.
Notice: Purchase of this instrument conveys a limited, non-transferable immunity from suit for the purchaser's
own internal research and development and applied fields other than human in vitro diagnostics under one or
more of Canadian Patent 1,339,653, U.S. Patent 5,475,610 (claims 160-163 only) or corresponding claims in
their unexpired non-U.S. counterparts owned by Applied Biosystems. No right is conveyed expressly, by
implication or by estoppel under any other patent claim, including under U.S. Patent No. 6,814,934 or any
global equivalent thereof, which claims thermal cycler apparatus and systems capable of real-time detection.
Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied
Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
Trademarks
“ALPS”, “DyNAmo”, “Finnpipette”, “Matrix”, “Maxima”, “Multidrop”, “Piko”, “PikoReal”, “Solaris”,
“Versette”, “Verso” and “UTW” are trademarks or registered trademarks of Thermo Fisher Scientific Inc. and
its subsidiaries.
All other trademarks and registered trademarks are the property of their respective holders.
Disclaimer
Thermo Fisher Scientific reserves the right to change its products and services at any time to incorporate
technological developments. This manual is subject to change without prior notice as part of a continuous
product development. Although this manual has been prepared with every precaution to ensure accuracy,
Thermo Fisher Scientific assumes no liability for any errors or omissions, nor for any damages resulting from
the application or use of this information. This manual supersedes all previous editions.
Remarks on screenshots
Screenshots may be slightly different on your system depending on the firmware version.
No liability for consequential damages
Thermo Fisher Scientific shall not be liable for any damages whatsoever arising out of the use or inability to
use this product.
Power failure
The system requires uninterrupted power supply in order to operate correctly. Thermo Fisher Scientific has no
responsibility whatsoever for system malfunctions arising from power failures.

About This User Manual
Intended users
Thermo Fisher Scientific Thermo Scientific PikoReal Real-Time PCR System User Manual 3
About This User Manual
The PikoReal Real-Time PCR System, as a standalone instrument or
with Thermo Scientific PikoReal Software, can be used in research
laboratories by professional personnel.
This user manual is for the following instruments: 24-well PikoReal
Real-Time PCR System (Cat. no. TCR0024) and 96-well PikoReal
Real-Time PCR System (Cat. no. TCR0096). It aims to give you the
information you need for:
●Reviewing safety precautions
●Installing the PikoReal Real-Time PCR System
●Operating the instrument
●Adjusting the settings and servicing the instrument
●Troubleshooting the instrument
This user manual also describes all the features and specifications of the
PikoReal Real-Time PCR System, and provides ordering information
for instruments, spare parts, reagents and consumables.
Read the manual in its entirety before operating the instrument.
Keep the user manual for future reference. The user manual is an
important part of the instrument and should be readily available.
For PikoReal Software related issues, refer to the Thermo Scientific
PikoReal Software User Manual (Cat. no. N12076).
For the latest information on products and services, visit our website:
http://www.thermoscientific.com/pikoreal
In our efforts to provide useful and appropriate documentation, we
would appreciate any comments you may have on this user manual to
your local Thermo Fisher Scientific representative.
Intended users
How to use this
user manual
For more
information

About This User Manual
Safety symbols and markings
4Thermo Scientific PikoReal Real-Time PCR System User Manual Thermo Fisher Scientific
These symbols are intended to draw your attention to particularly
important information and alert you to the presence of hazards.
The following symbols and markings appear on the type label and the
instrument itself.
Power on ▲
Power off ▲
Serial number ▲
Catalog number ▲
Date of manufacture ▲
Consult instructions for use ▲
WEEE symbol This product is required to comply with the European
Union’s Waste Electrical & Electronic Equipment (WEEE) Directive
2002/96/EC. ▲
Two labels with the following texts (Figure 2–3):
ATTENTION!
This is shipping position only! The other hole is operating position,
refer to the manual. ▲
ATTENTION!
Always insert the plug into the Piko first, and then insert the power
cord into the wall outlet. ▲
Safety symbols
and markings
Safety symbols and
markings used on the
PikoReal Real-Time PCR
System

About This User Manual
Safety instructions
Thermo Fisher Scientific Thermo Scientific PikoReal Real-Time PCR System User Manual 5
The following symbols and markings appear in this user manual.
Warning Risk of electric shock. ▲
Warning Biohazard risk. ▲
Warning Hot surface, risk of burns. ▲
Warning Risk of injury to the user(s). ▲
Caution Risk of damage to the instrument, other equipment or loss of
performance or function in a specific application. ▲
Note Marks a hint, important information that is useful in the
optimum operation of the system, or an item of interest. ▲
Read the User Manual Operating the PikoReal Real-Time PCR
System without first reading the entire User Manual may constitute a
risk to your health. Only a person capable of handling electrical
equipment should use the PikoReal Real-Time PCR System. NOT
FOR CHILDREN. ▲
Do Not Attempt to Repair the System Do not remove the cover of the
system. Do not try to repair or replace broken components – you put
yourself at risk for electrical shock. Removing the cover or
replacing/removing components will void the warranty. Contact
customer service or your local distributor if your system is not
functioning properly. ▲
Do Not Touch the Sample Block Certain components, including the
sample block and heated lid, will become excessively hot. Touching
these components may cause burns. ▲
Do Not Tamper with Electronics Coming into contact with the
electronics, even when the system is off or unplugged, may cause an
electrical shock or harm. ▲
Do Not Use Flammable, Hazardous or Radioactive Liquids with the
PikoReal Real-Time PCR System. ▲
W
arning and other
markings used in
the documentation
Safety
instructions

About This User Manual
Safety instructions
6Thermo Scientific PikoReal Real-Time PCR System User Manual Thermo Fisher Scientific

Contents
Thermo Fisher Scientific Thermo Scientific PikoReal Real-Time PCR System User Manual 7
Contents
Intended users...................................................................................3
How to use this user manual ............................................................. 3
For more information .......................................................................3
Safety symbols and markings.............................................................4
Safety symbols and markings used on the PikoReal Real-Time PCR
System............................................................................................ 4
Warning and other markings used in the documentation ...............5
Safety instructions ............................................................................. 5
Introduction to the PikoReal®Real-Time PCR System ........................ 11
Introduction to qPCR..................................................................... 13
Intended use.................................................................................... 13
General Description .................................................................................... 15
Instrument views............................................................................. 15
PikoReal user interface ....................................................................17
Status indicator ............................................................................ 17
Power supply...................................................................................17
PikoReal Software ...........................................................................18
Block formats..................................................................................18
Consumables................................................................................... 18
UTW technology ......................................................................... 19
Reagents.......................................................................................... 20
Dye calibration................................................................................ 21
Dispensers....................................................................................... 21
Heat sealers .....................................................................................21
Installation .................................................................................................... 23
What to do upon delivery ...............................................................23
Unpacking the instrument ........................................................... 23
Checking delivery for completeness and damage ..........................23
Environmental requirements ........................................................ 24
Air flow.....................................................................................25
Installation steps..............................................................................25
Connecting the power supply cable..............................................25
Connecting to the network ..........................................................26
Connecting to the data port ......................................................... 26
Warnings and cautions.................................................................... 26
Electrical ......................................................................................26
Operational check ........................................................................... 27
Installing PikoReal Software............................................................ 27
Chapter 1
Chapter 2
Chapter 3

Contents
8Thermo Scientific PikoReal Real-Time PCR System User Manual Thermo Fisher Scientific
Routine Operation ........................................................................................29
Switching on ................................................................................... 29
Status indicator ............................................................................... 29
Main menu .....................................................................................29
Running a protocol ......................................................................... 30
Monitoring a run ............................................................................32
Status screen.................................................................................32
Time screen..................................................................................33
Functions in the screens ............................................................... 33
Tools menu..................................................................................... 34
System info ..................................................................................34
Diagnostics ..................................................................................35
Lid ............................................................................................35
Sensors ...................................................................................... 36
Display...................................................................................... 36
Clock ........................................................................................37
Network....................................................................................... 37
Service.......................................................................................... 38
Logfiles......................................................................................... 38
Park instrument ...........................................................................38
Run data to USB.......................................................................... 39
Shutting down ................................................................................ 40
Maintenance .................................................................................................41
Regular and preventive maintenance ............................................... 41
Maintenance checklist .................................................................. 41
Instrument care ............................................................................ 41
Cleaning of the instrument .............................................................42
Cleaning the block and wells........................................................43
Cleaning the heat sink..................................................................43
Disposal of materials .......................................................................44
Decontamination procedure............................................................ 44
Packing for service........................................................................... 45
Maintaining a system log.................................................................46
Disposal of the instrument .............................................................. 46
Technical Specifications ...........................................................................47
Instrument specifications.................................................................47
Safety specifications......................................................................... 48
In conformity with the requirements............................................ 48
Troubleshooting Guide................................................................................51
Handling abnormal situations ......................................................... 51
Power failure ...................................................................................51
Warning and error codes................................................................. 51
Chapter 4
Chapter 5
Chapter 6
Chapter 7