Thermo Scientific BIOLiner Multifuge X3 User manual

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Thermo Scientific
BIOLiner™
Instruction Manual
50119954-e• 07/ 2020

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This product is subject to the regulations of the EU Waste Electrical & Electronic
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Thermo Scientific 75003667 BIOLiner i
T
Chapter 1 Rotor Specifications ..............................................................................................................1-1
Technical Data ........................................................................................................1-2
Chapter 2 Accessories .............................................................................................................................2-1
Chapter 3 AutoLock™.............................................................................................................................3-1
Rotor Installation .....................................................................................................3-2
Removing the Rotor .................................................................................................3-3
Aerosol-tight Rotors .................................................................................................3-3
Chapter 4 Rotor Loading ..........................................................................................................................4-1
Before a Run ............................................................................................................4-2
Proper Loading .........................................................................................................4-2
Improper Loading ....................................................................................................4-3
Maximum Loading ...................................................................................................4-3
Cycle Counter ..........................................................................................................4-3
Chapter 5 Aerosol-tight Applications ...................................................................................................5-1
Basic Principles .........................................................................................................5-2
Fill Level ...................................................................................................................5-2
Checking the aerosol Tightness ................................................................................5-2
Table of Contents
Foreword ..................................................................................................................................iii
Scope of Supply ........................................................................................................................iii
Precautions ...............................................................................................................................iii
Autoclaving .............................................................................................................. 6-5
Service of Thermo Fisher Scientific .......................................................................... 6-5
RCF-Values .................................................................................................................A-1
RCF-Values BIOLiner bucket ..............................................................................A-1
RCF-Values Microplate Carrier ...........................................................................A-2
Chemical Compatibility Chart .......................................................................................B-1
Chapter 6 Maintenance and Care ..........................................................................................................6-1
Cleaning Intervals .....................................................................................................6-2
Cleaning ...................................................................................................................6-2
Disinfection ..............................................................................................................6-3
Decontamination .....................................................................................................6-4

Thermo Scientific 75003667 BIOLiner iii
F
Foreword
Before starting to use the rotor, read through this instruction manual carefully and follow the
instructions.
The information contained in this instruction manual is the property of Thermo Fisher Scientific; it
is forbidden to copy or pass on this information without explicit approval.
Failure to follow the instructions and safety information in this instruction manual will result in the
expiration of the seller’s warranty.
Scope of Supply
If any parts are missing, please contact the nearest Thermo Fisher Scientific representative.
Precautions
In order to ensure safe operation of the BIOLiner™, the following general safety regulations must be
followed:
•Do not use rotors which show any signs of corrosion and / or cracks. Do not touch the electronic
components of the rotor and do not make any changes to the electronic or mechanical
components.
•Use only with rotors which have been loaded properly.
•Never overload the rotor.
Article Number Quantity Check
75003602 BIOLiner™1
76003500 Rubber seal grease 1
70009824 Anti-corrosion oil 1
75003786 Bolt grease 1
50119954 Instruction Manual 1

Foreword
iv 75003667 BIOLiner Thermo Scientific
•Use only accessories which have been approved by Thermo Fisher Scientific. Exceptions to this
rule are commercially available glass or plastic centrifuge tubes, provided they have been approved
for the speed or the RCF value of the rotor.
•Please observe the safety instructions.
Please pay particular attention to the following aspects:
•Rotor installation: Check that the rotor is locked properly into place before operating the
centrifuge.
•Always balance the samples.
Maximum sample density at maximum speed: 1.2 g
cm3
-----------
This symbol refers to general hazards.
ATTENTION means that material damage could occur.
WARNING means that injuries or material damage or contamination could occur.
This symbol refers to biological hazards.
Observe the information contained in the instruction manual to keep yourself and your
environment safe.

Thermo Scientific 75003667 BIOLiner 1-1
1
Rotor Specifications
Contents
•“Technical Data” on page 1-2

1Rotor Specifications
Technical Data
1-2 75003667 BIOLiner Thermo Scientific
Technical Data
Table 1-1. 230 V, 50 / 60 Hz, Rectangular buckets 75003668
Centrifuge Multifuge X3 Multifuge X3 F Megafuge 40
Catalog # 75004500 75004530 75004503
Weight empty [kg] 7.3 7.3 7.3
Max. cycle number 55000 55000 55000
Maximum permissible load [g] 4 x 750 4 x 750 4 x 750
Maximum speed nmax [rpm] 3500 3500 3500
Maximum RCF value at nmax 2739 2739 2739
Max. / min. radius [cm] 20 / 10.5 20 / 10.5 20 / 10.5
Pitch angle [°] 90 90 90
Accel. / braking time [s] 55 / 55 55 / 55 55 / 55
Sample heating at nmax [°C] referred to
ambient temperature of 23-25 °C, running
time 120 minutes
888
Aerosol-tight*yes yes yes
Permissible temperature range autoclavable
(cycle number) °C
121 121 121
*Tested by HPA, Porton-down, UK
Centrifuge Multifuge X3R Multifuge X3 FR Megafuge 40R
Catalog # 75004515 75004536 75004518
Weight empty [kg] 7.3 7.3 7.3
Max. cycle number 55000 55000 55000
Maximum permissible load [g] 4 x 750 4 x 750 4 x 750
Maximum speed nmax [rpm] 3500 3500 3500
Maximum RCF value at nmax 2739 2739 2739
Max. / min. radius [cm] 20 / 10.5 20 / 10.5 20 / 10.5
Pitch angle [°] 90 90 90
Accel. / braking time [s] 55 / 55 55 / 55 55 / 55
Minimum temperature at nmax [°C] relating to
ambient temperature 23-25 °C
<0 <0 <0
Speed at 4 °C 3500 3500 3500
Aerosol-tight *yes yes yes
Permissible temperature range autoclavable
(cycle number) °C
121 121 121
*Tested by HPA, Porton-down, UK

1 Rotor Specifications
Technical Data
Thermo Scientific 75003667 BIOLiner 1-3
Table1-2. 230 V, 50 / 60 Hz, Microplate carriers 75003670
Centrifuge Multifuge X3 Multifuge X3 F Megafuge 40
Catalog # 75004500 75004531 75004503
Weight empty [kg] 7.3 7.3 7.3
Max. cycle number 90000 90000 90000
Maximum permissible load [g] 4 x 750 4 x 750 4 x 750
Maximum speed nmax [rpm] 3500 3500 3500
Maximum RCF value at nmax 2451 2451 2451
Max. / min. radius [cm] 17.9 / 10.5 17.9 / 10.5 17.9 / 10.5
Pitch angle [°] 90 90 90
Accel. / braking time [s] 40 / 50 40 / 50 40 / 50
Sample heating at nmax [°C] referred to
ambient temperature of 23-25 °C, running time
120 minutes
888
Aerosol-tight*yes yes yes
Permissible temperature range autoclavable
(cycle number) °C
121 121 121
*Tested by HPA, Porton-down, UK
Centrifuge Multifuge X3R Multifuge X3 FR Megafuge 40R
Catalog # 75004515 75004536 75004518
Weight empty [kg] 7.3 7.3 7.3
Max. cycle number 90000 90000 90000
Maximum permissible load [g] 4 x 750 4 x 750 4 x 750
Maximum speed nmax [rpm] 3500 3500 3500
Maximum RCF value at nmax 2451 2451 2451
Max. / min. radius [cm] 17.9 / 10.5 17.9 / 10.5 17.9 / 10.5
Pitch angle [°] 90 90 90
Accel. / braking time [s] 40 / 50 40 / 50 40 / 50
Minimum temperature at nmax [°C] relating to
ambient temperature 23-25 °C
<0 <0 <0
Speed at 4 °C 3500 3500 3500
Aerosol-tight *yes yes yes
Permissible temperature range autoclavable
(cycle number) °C
121 121 121
*Tested by HPA, Porton-down, UK

1Rotor Specifications
Technical Data
1-4 75003667 BIOLiner Thermo Scientific
Table 1-3. 120 V, 60 Hz, Rectangular buckets 75003668
Centrifuge Multifuge X3 Multifuge X3 F Megafuge 40
Catalog # 75004501 75004531 75004504
Weight empty [kg] 7.3 7.3 7.3
Max. cycle number 55000 55000 55000
Maximum permissible load [g] 4 x 750 4 x 750 4 x 750
Maximum speed nmax [rpm] 3500 3500 3500
Maximum RCF value at nmax 2739 2739 2739
Max. / min. radius [cm] 20 / 10.5 20 / 10.5 20 / 10.5
Pitch angle [°] 90 90 90
Accel. / braking time [s] 75 / 60 75 / 60 75 / 60
Sample heating at nmax [°C] referred to
ambient temperature of 23-25 °C, running
time 120 minutes
888
Aerosol-tight*yes yes yes
Permissible temperature range autoclavable
(cycle number) °C
121 121 121
*Tested by HPA, Porton-down, UK
Centrifuge Multifuge X3R Multifuge X3 FR Megafuge 40R
Catalog # 75004516 75004537 75004519
Weight empty [kg] 7.3 7.3 7.3
Max. cycle number 55000 55000 55000
Maximum permissible load [g] 4 x 750 4 x 750 4 x 750
Maximum speed nmax [rpm] 3500 3500 3500
Maximum RCF value at nmax 2739 2739 2739
Max. / min. radius [cm] 20 / 10.5 20 / 10.5 20 / 10.5
Pitch angle [°] 90 90 90
Accel. / braking time [s] 70 / 60 70 / 60 70 / 60
Minimum temperature at nmax [°C] relating to
ambient temperature 23-25 °C
222
Speed at 4 °C 3500 3500 3500
Aerosol-tight*yes yes yes
Permissible temperature range autoclavable
(cycle number) °C
121 121 121
*Tested by HPA, Porton-down, UK

1 Rotor Specifications
Technical Data
Thermo Scientific 75003667 BIOLiner 1-5
Table1-4. 120 V, 60 Hz, Microplate carriers 75003670
Centrifuge Multifuge X3 Multifuge X3 F Megafuge 40
Catalog # 75004501 75004531 75004504
Weight empty [kg] 7.3 7.3 7.3
Max. cycle number 90000 90000 90000
Maximum permissible load [g] 4 x 750 4 x 750 4 x 750
Maximum speed nmax [rpm] 3500 3500 3500
Maximum RCF value at nmax 2451 2451 2451
Max. / min. radius [cm] 17.9 / 10.5 17.9 / 10.5 17.9 / 10.5
Pitch angle [°] 90 90 90
Accel. / braking time [s] 60 / 50 60 / 50 60 / 50
Sample heating at nmax [°C] referred to
ambient temperature of 23-25 °C, running time
120 minutes
888
Aerosol-tight*yes yes yes
Permissible temperature range autoclavable
(cycle number) °C
121 121 121
*Tested by HPA, Porton-down, UK
Centrifuge Multifuge X3R Multifuge X3 FR Megafuge 40R
Catalog # 75004516 75004537 75004519
Weight empty [kg] 7.3 7.3 7.3
Max. cycle number 90000 90000 90000
Maximum permissible load [g] 4 x 750 4 x 750 4 x 750
Maximum speed nmax [rpm] 3500 3500 3500
Maximum RCF value at nmax 2451 2451 2451
Max. / min. radius [cm] 17.9 / 10.5 17.9 / 10.5 17.9 / 10.5
Pitch angle [°] 90 90 90
Accel. / braking time [s] 50 / 50 50 / 50 50 / 50
Minimum temperature at nmax [°C] relating to
ambient temperature 23-25 °C
222
Speed at 4 °C 3500 3500 3500
Aerosol-tight*yes yes yes
Permissible temperature range autoclavable
(cycle number) °C
121 121 121
*Tested by HPA, Porton-down, UK

1Rotor Specifications
Technical Data
1-6 75003667 BIOLiner Thermo Scientific
Table 1-5. 230 V, 50 / 60 Hz, Rectangular buckets 75003668
Centrifuge Sorvall Legend XT Sorvall Legend XF Sorvall ST 40
Catalog # 75004505 75004532 75004509
Weight empty [kg] 7.3 7.3 7.3
Max. cycle number 55000 55000 55000
Maximum permissible load [g] 4 x 750 4 x 750 4 x 750
Maximum speed nmax [rpm] 3500 3500 3500
Maximum RCF value at nmax 2739 2739 2739
Max. / min. radius [cm] 20 / 10.5 20 / 10.5 20 / 10.5
Pitch angle [°] 90 90 90
Accel. / braking time [s] 55 / 55 55 / 55 55 / 55
Sample heating at nmax [°C] referred to
ambient temperature of 23-25 °C, running
time 120 minutes
888
Aerosol-tight*yes yes yes
Permissible temperature range autoclavable
(cycle number) °C
121 121 121
*Tested by HPA, Porton-down, UK
Centrifuge Sorvall Legend XTR Sorvall Legend XFR Sorvall ST 40R
Catalog # 75004520 75004538 75004524
Weight empty [kg] 7.3 7.3 7.3
Max. cycle number 55000 55000 55000
Maximum permissible load [g] 4 x 750 4 x 750 4 x 750
Maximum speed nmax [rpm] 3500 3500 3500
Maximum RCF value at nmax 2739 2739 2739
Max. / min. radius [cm] 20 / 10.5 20 / 10.5 20 / 10.5
Pitch angle [°] 90 90 90
Accel. / braking time [s] 50 / 55 50 / 55 50 / 55
Minimum temperature at nmax [°C] relating to
ambient temperature 23-25 °C
<0 <0 <0
Speed at 4 °C 3500 3500 3500
Aerosol-tight*yes yes yes
Permissible temperature range autoclavable
(cycle number) °C
121 121 121
*Tested by HPA, Porton-down, UK

1 Rotor Specifications
Technical Data
Thermo Scientific 75003667 BIOLiner 1-7
Table1-6. 230 V, 50 / 60 Hz, Microplate carriers 75003670
Centrifuge Sorvall Legend XT Sorvall Legend XF Sorvall ST 40
Catalog # 75004505 75004532 75004509
Weight empty [kg] 7.3 7.3 7.3
Max. cycle number 90000 90000 90000
Maximum permissible load [g] 4 x 750 4 x 750 4 x 750
Maximum speed nmax [rpm] 3500 3500 3500
Maximum RCF value at nmax 2451 2451 2451
Max. / min. radius [cm] 17.9 / 10.5 17.9 / 10.5 17.9 / 10.5
Pitch angle [°] 90 90 90
Accel. / braking time [s] 40 / 50 40 / 50 40 / 50
Sample heating at nmax [°C] referred to
ambient temperature of 23-25 °C, running time
120 minutes
888
Aerosol-tight*yes yes yes
Permissible temperature range autoclavable
(cycle number) °C
121 121 121
*Tested by HPA, Porton-down, UK
Centrifuge Sorvall Legend XTR Sorvall Legend XFR Sorvall ST 40R
Catalog # 75004520 75004538 75004524
Weight empty [kg] 7.3 7.3 7.3
Max. cycle number 90000 90000 90000
Maximum permissible load [g] 4 x 750 4 x 750 4 x 750
Maximum speed nmax [rpm] 3500 3500 3500
Maximum RCF value at nmax 2451 2451 2451
Max. / min. radius [cm] 17.9 / 10.5 17.9 / 10.5 17.9 / 10.5
Pitch angle [°] 90 90 90
Accel. / braking time [s] 40 / 50 40 / 50 40 / 50
Minimum temperature at nmax [°C] relating to
ambient temperature 23-25 °C
<0 <0 <0
Speed at 4 °C 3500 3500 3500
Aerosol-tight*yes yes yes
Permissible temperature range autoclavable
(cycle number) °C
121 121 121
*Tested by HPA, Porton-down, UK

1Rotor Specifications
Technical Data
1-8 75003667 BIOLiner Thermo Scientific
Table 1-7. 120 V, 60 Hz, Rectangular buckets 75003668
Centrifuge Sorvall Legend XT Sorvall Legend XF Sorvall ST 40
Catalog # 75004506 75004533 75004510
Weight empty [kg] 7.3 7.3 7.3
Max. cycle number 55000 55000 55000
Maximum permissible load [g] 4 x 750 4 x 750 4 x 750
Maximum speed nmax [rpm] 3500 3500 3500
Maximum RCF value at nmax 2739 2739 2739
Max. / min. radius [cm] 20 / 10.5 20 / 10.5 20 / 10.5
Pitch angle [°] 90 90 90
Accel. / braking time [s] 75 / 60 75 / 60 75 / 60
Sample heating at nmax [°C] referred to
ambient temperature of 23-25 °C, running
time 120 minutes
888
Aerosol-tight*yes yes yes
Permissible temperature range autoclavable
(cycle number) °C
121 121 121
*Tested by HPA, Porton-down, UK
Centrifuge Sorvall Legend XTR Sorvall Legend XFR Sorvall ST 40R
Catalog # 75004521 75004539 75004525
Weight empty [kg] 7.3 7.3 7.3
Max. cycle number 55000 55000 55000
Maximum permissible load [g] 4 x 750 4 x 750 4 x 750
Maximum speed nmax [rpm] 3500 3500 3500
Maximum RCF value at nmax 2739 2739 2739
Max. / min. radius [cm] 20 / 10.5 20 / 10.5 20 / 10.5
Pitch angle [°] 90 90 90
Accel. / braking time [s] 70 / 60 70 / 60 70 / 60
Minimum temperature at nmax [°C] relating
to ambient temperature 23-25 °C
222
Speed at 4 °C 3500 3500 3500
Aerosol-tight*yes yes yes
Permissible temperature range autoclavable
(cycle number) °C
121 121 121
*Tested by HPA, Porton-down, UK

1 Rotor Specifications
Technical Data
Thermo Scientific 75003667 BIOLiner 1-9
Table1-8. 120 V, 60 Hz Microplate carriers 75003670
Centrifuge Sorvall Legend XT Sorvall Legend XF Sorvall ST 40
Catalog # 75004506 75004533 75004510
Weight empty [kg] 7.3 7.3 7.3
Max. cycle number 90000 90000 90000
Maximum permissible load [g] 4 x 750 4 x 750 4 x 750
Maximum speed nmax [rpm] 3500 3500 3500
Maximum RCF value at nmax 2451 2451 2451
Max. / min. radius [cm] 17.9 / 10.5 17.9 / 10.5 17.9 / 10.5
Pitch angle [°] 90 90 90
Accel. / braking time [s] 60 / 50 60 / 50 60 / 50
Sample heating at nmax [°C] referred to
ambient temperature of 23-25 °C, running time
120 minutes
888
Aerosol-tight*yes yes yes
Permissible temperature range autoclavable
(cycle number) °C
121 121 121
*Tested by HPA, Porton-down, UK
Centrifuge Sorvall Legend XTR Sorvall Legend XFR Sorvall ST 40R
Catalog # 75004521 75004539 75004525
Weight empty [kg] 7.3 7.3 7.3
Max. cycle number 90000 90000 90000
Maximum permissible load [g] 4 x 750 4 x 750 4 x 750
Maximum speed nmax [rpm] 3500 3500 3500
Maximum RCF value at nmax 2451 2451 2451
Max. / min. radius [cm] 17.9 / 10.5 17.9 / 10.5 17.9 / 10.5
Pitch angle [°] 90 90 90
Accel. / braking time [s] 50 / 50 50 / 50 50 / 50
Minimum temperature at nmax [°C] relating to
ambient temperature 23-25 °C
222
Speed at 4 °C 3500 3500 3500
Aerosol-tight*yes yes yes
Permissible temperature range autoclavable
(cycle number) °C
121 121 121
*Tested by HPA, Porton-down, UK

1Rotor Specifications
Technical Data
1-10 75003667 BIOLiner Thermo Scientific
Table 1-9. 100 V, 50 / 60 Hz, Rectangular buckets 75003668
Centrifuge Sorvall Legend XT Sorvall Legend XF
Catalog # 75004508 75004535
Weight empty [kg] 7.3 7.3
Max. cycle number 55000 55000
Maximum permissible load [g] 4 x 750 4 x 750
Maximum speed nmax [rpm] 3500 3500
Maximum RCF value at nmax 2739 2739
Max. / min. radius [cm] 20 / 10.5 20 / 10.5
Pitch angle [°] 90 90
Accel. / braking time [s] 75 / 60 75 / 60
Sample heating at nmax [° C] referred to
ambient temperature of 23-25 °C, running
time 120 minutes
88
Aerosol-tight*yes yes
Permissible temperature range autoclavable
(cycle number) °C
121 121
*Tested by HPA, Porton-down, UK
Centrifuge Sorvall Legend XTR Sorvall Legend XFR
Catalog # 75004523 75004541
Weight empty [kg] 7.3 7.3
Max. cycle number 55000 55000
Maximum permissible load [g] 4 x 750 4 x 750
Maximum speed nmax [rpm] 3500 3500
Maximum RCF value at nmax 2739 2739
Max. / min. radius [cm] 20 / 10.5 20 / 10.5
Pitch angle [°] 90 90
Accel. / braking time [s] 70 / 60 70 / 60
Minimum temperature at nmax [°C] relating
to ambient temperature 23-25 °C
22
Speed at 4 °C 3500 3500
Aerosol-tight*yes yes
Permissible temperature range autoclavable
(cycle number) °C
121 121
*Tested by HPA, Porton-down, UK

1Rotor Specifications
Technical Data
Table 1-9. 100 V, 50 / 60 Hz, Microplate carriers 75003670
Centrifuge
Catalog #
Weight empty [kg]
Max. cycle number
Maximum permissible load [g]
Maximum speed nmax [rpm]
Maximum RCF value at nmax
Max. / min. radius [cm]
Pitch angle [°]
Accel. / braking time [s]
Sample heating at nmax [° C] referred to
ambient temperature of 23-25 °C, running
time 60 minutes
Aerosol-tight*
Permissible temperature range autoclavable
(cycle number) °C
*Tested by HPA, Porton-down, UK
Centrifuge
Catalog #
Weight empty [kg]
Max. cycle number
Maximum permissible load [g]
Maximum speed nmax [rpm]
Maximum RCF value at nmax
Max. / min. radius [cm]
Pitch angle [°]
Accel. / braking time [s]
Aerosol-tight*
Permissible temperature range autoclavable
(cycle number) °C
*Tested by HPA, Porton-down, UK
Thermo Scientific 75003667 BIOLiner 1-11
Sorvall Legend XT Sorvall Legend XF Sorvall ST 40
75004507 75004534 75004511
7.3 7.3 7.3
90000 90000 90000
4 x 500 4 x 500 4 x 500
3500 3500 3500
2739 2739 2739
17.9 / 14.6 17.9 / 14.6 17.9 / 14.6
90 90 90
60 / 50 60 / 50 60 / 50
88
8
yes yes yes
121 121 121
Sorvall Legend XTR Sorvall Legend XFR Sorvall ST 40R
75004522 75004540 75004526
7.3 7.3 7.3
90000 90000 90000
4 x 500 4 x 500 4 x 500
3500 3500 3500
2739 2739 2739
17.9 / 14.6 17.9 / 14.6 17.9 / 14.6
90 90 90
50 / 50 50 / 50 50 / 50
yes yes yes
121 121 121

1Rotor Specifications
Technical Data
Table 1-10. 230 V, 50 / 60 Hz, Rectangular buckets 75003668
Centrifuge Thermo Scientific SL 40 Thermo Scientific SL 40 F
Catalog # 75004512 75004542
Weight empty [kg] 7.3 7.3
Max. cycle number 55000 55000
Maximum permissible load [g] 4 x 750 4 x 750
Maximum speed nmax [rpm] 3500 3500
Maximum RCF value at nmax 2739 2739
Max. / min. radius [cm] 20 / 10.5 20 / 10.5
Pitch angle [°] 90 90
Accel. / braking time [s] 55 / 50 55 / 55
Sample heating at nmax [°C] referred to
ambient temperature of 23-25 °C, running
time 120 minutes
88
Aerosol-tight*yes yes
Permissible temperature range autoclavable
(cycle number) °C
121 121
*Tested by HPA, Porton-down, UK
Centrifuge Thermo Scientific SL 40R Thermo Scientific SL 40 FR
Catalog # 75004527 75004543
Weight empty [kg] 7.3 7.3
Max. cycle number 55000 55000
Maximum permissible load [g] 4 x 750 4 x 750
Maximum speed nmax [rpm] 3500 3500
Maximum RCF value at nmax 2739 2739
Max. / min. radius [cm] 20 / 10.5 20 / 10.5
Pitch angle [°] 90 90
Accel. / braking time [s] 55 / 50 55 / 55
Minimum temperature at nmax [°C]
relating to ambient temperature 23-25 °C
<0 <0
Speed at 4 °C 3500 3500
Aerosol-tight*yes yes
Permissible temperature range
autoclavable (cycle number) °C
121 121
*Tested by HPA, Porton-down, UK
1-1275003667 BIOLiner Thermo Scientific