Thermo Scientific Cryofuge 8 User manual

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Thermo Scientific
Cryofuge 8 / 16
Centrifuge and Heavy Duty Centrifuge
Instruction Manual
50149611-k • 2022-02

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Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges
Table of Contents
Preface .................................................vi
Items Supplied ....................................................vi
Intended Use for Cryofuge 8 / 16 and Cryofuge 16 Heavy Duty ............vii
Medical ...........................................................................................................................vii
In Vitro Diagnostics ..........................................................................................................vii
Signal Words and Colors ...........................................viii
Precautions......................................................viii
Symbols used on the Centrifuge and its Components.....................xi
Symbols used in the Manual ........................................xiii
Transport and Set Up .....................................I–1
1. Location ......................................................I–1
2. Transporting...................................................I–2
3. Setting Up ....................................................I–3
Setting Up...................................................................................................................... I–3
Level the Centrifuge ....................................................................................................... I–4
4. Mains Connection ..............................................I–5
5. Storage.......................................................I–5
6. Shipping......................................................I–6
ThermoScientific
Centri-Touch User Interface............................... II–1
Status .........................................................II–2
Run Parameters .................................................II–3
Control and Configuration .........................................II–3
Lighthouse Mode ................................................II–4
Operation .............................................III–1
1. Switch on the Centrifuge .......................................III–1
2. Open the Centrifuge Door .......................................III–1
3. Install a Rotor ................................................III–1
3. 1. Before Installation.................................................................................................III–2

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Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges
3. 2. Rotor Installation ..................................................................................................III–3
4. Maximum Loading.............................................III–8
Use of Tubes and Consumables.....................................................................................III–9
5. Close the Centrifuge Door.......................................III–9
6. Entering Parameters ...........................................III–9
6. 1. Acceleration / Deceleration Profiles .......................................................................III–9
6. 2. Preselect Speed / RCF-Value ................................................................................III–9
6. 3. Runtime Preselection..........................................................................................III–10
6. 4. Preselect Temperature ........................................................................................III–10
6. 5. Pre-warm or Pre-cool the Centrifuge ...................................................................III–10
7. Programs .................................................. III–11
8. Centrifugation .............................................. III–12
8. 1. Start ..................................................................................................................III–12
8. 2. Stop ..................................................................................................................III–12
8. 3. Using the Drain Box............................................................................................III–13
9. Remove a Rotor ............................................. III–14
10. Switch off the Centrifuge .................................... III–15
Maintenance and Care ...................................IV–1
1. Cleaning Intervals ............................................ IV–1
2. Basics...................................................... IV–1
2. 1. Rotor and Components Inspection........................................................................ IV–2
3. Cleaning .................................................... IV–3
3. 1. Touchscreen ....................................................................................................... IV–4
3. 2. Filter Mat............................................................................................................ IV–5
4. Disinfection ................................................. IV–6
5. Decontamination ............................................. IV–6
6. Autoclaving ................................................. IV–7
7. Maintenance ................................................ IV–8
7. 1. Preventive Maintenance....................................................................................... IV–8
7. 2. Service ............................................................................................................... IV–8
8. Shipping and Disposal ........................................ IV–9
Troubleshooting .........................................V-1
1. Mechanical Emergency Door Release ............................. V-1
2. Ice Formation................................................. V-2

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Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges
3. Troubleshooting by Guide ....................................... V-3
4. Information for the Customer Service ............................. V-4
Technical Specifications ................................. A–1
1. Directives, Standards and Guidelines .............................A–2
2. Refrigerants ..................................................A–3
3. Mains Supply .................................................A–3
4. Components..................................................A–4
Rotors ................................................ B–1
Items Supplied for Rotors .........................................B–1
HAEMAFlex 6 Rotor ..............................................B–2
HAEMAFlex 6 Rotor ..............................................B–3
HAEMAFlex 6 Rotor ..............................................B–4
HAEMAFlex 8 Rotor ..............................................B–5
HAEMAFlex 8 Rotor ..............................................B–6
HAEMAFlex 8 Rotor ..............................................B–7
HAEMAFlex 12 Rotor .............................................B–8
HAEMAFlex 12 Rotor .............................................B–9
HAEMAFlex 16 Rotor ............................................B–10
HAEMAFlex 16 Rotor ............................................B–11
Chemical Compatibility Chart ............................. C–1

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Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges
Table of Figures
Figure I–1: Centrifuge clearance, example with bar code scanner ................................... I–2
Figure I–2: Position of the bubble in the water level........................................................ I–4
Figure I–3: Mains connection on the backside of the centrifuge ...................................... I–5
Figure III–1: Overview centrifuge and rotor, e.g. HAEMAFlex 12......................................III–2
Figure III–2: Align windshield knob and drive shaft .......................................................III–3
Figure III–3: Identify the circular marking, e.g. HAEMAFlex 8 and 12 .............................III–3
Figure III–4: Rotor locking tool ......................................................................................III–4
Figure III–5: Put the rotor locking tool............................................................................III–4
Figure III–6: Tighten the rotor to the drive shaft.............................................................III–5
Figure III–7: Lubricate trunnion bolts, e.g. HAEMAFlex 12 ..............................................III–5
Figure III–8: Install buckets into rotor, e.g. HAEMAFlex 12..............................................III–6
Figure III–9: Correctly assembled round bucket (75003836/75003936, not 75003964) .III–6
Figure III–10: Correct Loading.......................................................................................III–7
Figure III–11: Incorrect Loading.....................................................................................III–7
Figure III–12: Insert windshield lid into holder ...............................................................III–8
Figure III–13: Select program......................................................................................III–11
Figure III–14: Drain box (75007730)...........................................................................III–13
Figure III–15: Installing the drain box (75007730)........................................................III–13
Figure III–16: Remove the plug ...................................................................................III–13
Figure III–17: Remove the rotor from the drive shaft. ...................................................III–14
Figure IV–1: Cleaning rotor bolts and bucket bearing surfaces ...................................... IV–4
Figure V–1: Marking on windshield lid ...........................................................................V-1
Figure V–2: Position of the mechanical emergency door release......................................V-2
Figure V–3: Example for an error message.....................................................................V-3

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Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges
Table of Tables
Table Preface–1: Items Supplied.......................................................................................vi
Table Preface–2: Signal Words and Colors ...................................................................... viii
Table Preface–3: Symbols used on the Centrifuge and its Components ............................ xiii
Table Preface–4: Symbols used in the manual................................................................. xiii
Table I–1: Overview of necessary tools for setting up...................................................... I–3
Table V–1: Error messages............................................................................................V-3
Table A–1: Technical Data Thermo Scientific Cryofuge 8 / 16 and Cryofuge 16 Heavy DutyA–1
Table A–2: Directives, Standards and Guidelines Cryofuge 8 / 16 and Cryofuge 16 Heavy Duty
...........................................................................................................................A–2
Table A–3: Refrigerants used in the Cryofuge 8 / 16 and Cryofuge 16 Heavy Duty..........A–3
Table A–4: Mains Supply..............................................................................................A–3
Table A–5: Components ...............................................................................................A–5
Table B–1: Items supplied for rotors..............................................................................B–1
Table B–2: HAEMAFlex 6 Rotor with Single Blood Bag Buckets (oval)..............................B–2
Table B–3: HAEMAFlex 6 Rotor with Single Blood Bag Buckets (round)...........................B–3
Table B–4: HAEMAFlex 6 Rotor with Single Blood Bag Buckets with Filter Pack...............B–4
Table B–5: HAEMAFlex 8 Rotor with Single Blood Bag Buckets (oval)..............................B–5
Table B–6: HAEMAFlex 8 Rotor with Single Blood Bag Buckets (round)...........................B–6
Table B–7: HAEMAFlex 8 Rotor with Single Blood Bag Buckets with Filter Pack...............B–7
Table B–8: HAEMAFlex 12 Rotor with Double Blood Bag Buckets...................................B–8
Table B–9: HAEMAFlex 12 Rotor with Oval Buckets .......................................................B–9
Table B–10: HAEMAFlex 16 Rotor with Double Blood Bag Buckets...............................B–10
Table B–11: HAEMAFlex 16 Rotor with Oval Buckets ...................................................B–11
Table C–1: Chemical Compatibility..............................................................................C–10

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges
Preface
vi
Preface
Before starting to use the centrifuge, read through this instruction manual carefully and follow the instructions.
Failure to follow the instructions and safety information in this instruction manual will result in the expiration of the sellers
Warranty.
Items Supplied
The centrifuges are supplied without a rotor. The items supplied with a rotor are listed: [à 2 B–1]
Article No. Item Quantity
Centrifuge 1
75007671 Thermo Scientific Cryofuge 8,
200, 208, 220, 230, 240 V ±10 %, 50 / 60 Hz, Single phase
75007672 Thermo Scientific Cryofuge 8,
380, 400, 415 V ±10 %, 50 Hz, 3-phase
75007673 Thermo Scientific Cryofuge 16,
200, 208, 220, 230, 240 V ±10 %, 50 / 60 Hz, Single phase
75007674 Thermo Scientific Cryofuge 16,
380, 400, 415 V ±10 %, 50 Hz, 3-phase
75007678 Thermo Scientific Cryofuge 16 Heavy Duty,
380, 400, 415 V ±10 %, 50 Hz, 3-phase
Power Supply Cable 1
For single phase units with 200, 208, 220, 230, 240 V
20190357 IEC60309 32A-6h 3-pin blue, 200–250 V
20190358 NEMA 6-30P 30A, 200–250 V
20190359 IEC60309 32A-6h 5-pin red (3P+N+PE),
220/380 V; 230/400 V; 240/415 V
20190364 NEMA L6-30P 30A, 200–205 V
For 3-phase units with 380/400/415 V
20190360 IEC60309 16A-6h 5 pin red (3P+N+PE),
220 / 380 V; 230 / 400 V; 240 / 415 V
20280119 Bubble Level 1
Instruction Manual 1
CD 1
Table Preface–1: Items Supplied
If any parts are missing, please contact the nearest Thermo Fisher Scientific representative.

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges
Preface
vii
Intended Use for Cryofuge 8 / 16 and Cryofuge 16
Heavy Duty
Medical
This centrifuge is intended for separation of blood into blood components using blood bags. The blood components are intended
to be used for human blood transfusion.
Clinical facilities and institutions are responsible for the process of human blood transfusion.
This intended use is limited to the associated rotors and components. Based on the above this centrifuge is a medical device per
European Directive 93/42/EEC. CE marked with Notified Body involvement.
In Vitro Diagnostics
This centrifuge system is intended for separation of blood into cell-free supernatant (plasma, serum) and cell-containing sediment.
Plasma and serum are intended to be used in In-Vitro Diagnostic (IVD) applications (assays).
Blood is used in numerous diagnostic tests such as hematological screening (e.g. measurement of free hemoglobulin),
immunological screening (e.g. measurement of thrombocytes levels) and cardiovascular system assessment.
Self declared, CE marked without Notified Body involvement.
Intended User
The centrifuge must be operated by trained personal only.
Trained personnel can be a clinical laboratory technologist, a medical laboratory technician or personal with similar education.
Associated Rotors and Components
The Thermo Scientific™Cryofuge 8 centrifuge can be operated with the Thermo Scientific™HAEMAFlex™6 and HAEMAFlex 8
rotor. The Thermo Scientific Cryofuge 16 and Thermo Scientific Cryofuge 16 Heavy Duty centrifuge can be operated with the
Thermo Scientific HAEMAFlex 12 and HAEMAFlex 16 rotor. For more details on buckets and further components: [à 2 A–4]
[à 2 B–1]

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges
Preface
viii
Signal Words and Colors
Signal Word and Color Degree of Hazard
WARNING Indicates a hazardous situation that, if not avoided, could result in death or serious injury.
CAUTION Indicates a hazardous situation that, if not avoided, could result in minor or moderate injury.
NOTICE Indicates information considered important, but not hazard-related.
Table Preface–2: Signal Words and Colors
Precautions
WARNING
Observe the safety instructions. Not following these instructions can cause
damage like harm by mechanical impact, electrical shock, infection and loss
of sample.
The centrifuge is to be used for its intended use only. Improper use can cause
damages, contamination, and injuries with fatal consequences.
The centrifuge must be operated by trained personnel only.
It is the obligation of the operator to make sure, that the proper protective clothing
is used. Mind the “Laboratory Biosafety Manual” of the World Health Organization
(WHO) and the regulations in your country.
As safety zone maintain a clear radius of at least 30 cm around the centrifuge. Do not
place any dangerous substances within this safety zone.
Set up in a well-ventilated environment, on a horizontally leveled and rigid surface with
adequate load-bearing capacity.
Do not modify the centrifuge and its accessories in any unauthorized way.
The centrifuge housing is not to be opened by the operator.
Thermo Fisher Scientific is not responsible for the process of human blood transfusion.
To ensure safe operation of this centrifuge regarding blood and blood components
you have to follow the regulations in your country.
WARNING
Risk of damage due to incorrect power supply.
Make sure that the centrifuge is plugged only into a power outlet that has been
properly grounded.

Thermo Scientific Cryofuge 8 / 16 Centrifuges and Heavy Duty Centrifuges
Preface
ix
WARNING
Risk from handling hazardous substances.
Especially when working with corrosive samples (salt solutions, acids, bases), the
components and the centrifuge chamber have to be cleaned thoroughly.
Do not centrifuge explosive or flammable materials or substances.
The centrifuge is neither inert nor protected against explosion. Never use the
centrifuge in an explosion-prone environment.
Do not centrifuge toxic or radioactive materials or any pathogenic micro-organisms
without suitable safety precautions.
If centrifuging any hazardous materials mind the “Laboratory Biosafety Manual” of
the World Health Organization (WHO) and any local regulations. When centrifuging
microbiological samples from the Risk Group II (according to the “Laboratory
Biosafety Manual” of the World Health Organization (WHO)), aerosol-tight biological
seals have to be used. Look on the internet page of the World Health Organization
(www.who.int) for the “Laboratory Biosafety Manual”. For materials in a higher risk
group, extra safety measures have to be taken.
If toxins or pathogenic substances have contaminated the centrifuge or its parts,
appropriate disinfection measures have to be taken. [à 2 IV–6]
Extreme care should be taken with highly corrosive substances which can cause
damage and impair the mechanical stability of the rotor. These should only be
centrifuged in fully sealed tubes.
If a hazardous situation occurs, turn off the power supply to the centrifuge and leave
the area immediately.
WARNING
Serious injuries can occur, if you touch a spinning rotor with your hands or
tools.
Never open the centrifuge door until the rotor has come to a complete stop and this
has been confirmed on the touchscreen.
The emergency door release may be used in emergencies only to recover the
samples from the centrifuge, e.g. during a power failure. [à 2 V-1]
Do not open the centrifuge, while it is running.
In any case of severe mechanical failure, such as rotor or bucket crash, the centrifuge
is not aerosol-tight.
In case of rotor failure the centrifuge can be damaged. Leave the room. Inform
customer service.
WARNING
Safety can be impaired by wrong loading and worn accessories.
Use only a properly installed rotor. [à 2 III–1]
Do not use rotors, buckets or components which show any signs of removed
protective coating, corrosion or cracks. Contact customer service for further advice
or inspections.
Use only with rotors which have been loaded properly.
Never overload the rotor.
Always balance the samples.
Use only rotors and components for this centrifuge which have been approved by
Thermo Fisher Scientific. Exceptions to this rule are commercially available glass or
plastic centrifuge lab ware, provided they have been designed to fit the rotor or the
adapter cavities and are approved for the speed or the RCF value of the rotor.
Make sure the rotor is locked properly into place before operating the centrifuge.