Thermo Scientific EASY-Spray Series User manual

EASY-Spray Series
Ion Source
User Guide
60053-97275 Revision B June 2018

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Release history: Rev A, April 2017; Rev B, June 2018
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Thermo Scientific EASY-Spray Series Ion Source User Guide v
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Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
Accessing Documentation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .ix
Viewing the Product Manuals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x
Viewing Online User Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x
Providing Documentation Feedback. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x
Compatible Mass Spectrometers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xi
Special Notices, Symbols, and Cautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xi
Safety Precautions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiii
Regulatory Compliance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiii
Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiv
Chapter 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
Advantages of Nanoelectrospray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Functional Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Source Housing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Temperature Controller PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Controller Power Supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Chapter 2 Installing the EASY-Spray Series Ion Source . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7
Connecting Power to the Ion Source. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Powering the Ion Source for an EASY-nLC 1200/MS System. . . . . . . . . . . . . 8
Powering the Ion Source for Other nanoLC/MS Systems . . . . . . . . . . . . . . . . 9
Attaching the Ion Source. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Adjusting the Emitter Tip Position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Removing the EASY-Spray Series Source. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Chapter 3 Installing the Camera and LCD Monitor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15
Installing the USB Camera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Installing the Analog Camera and LCD Monitor . . . . . . . . . . . . . . . . . . . . . . . 17
Adjusting the Video Picture. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Chapter 4 Connecting the EASY-Spray Column Plumbing. . . . . . . . . . . . . . . . . . . . . . . . . . . .19
Tools and Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Installing the EASY-Spray Column for the First Time. . . . . . . . . . . . . . . . . . . . 20
Setting the Column Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Contents

Contents
vi EASY-Spray Series Ion Source User Guide Thermo Scientific
Using the nanoViper Fitting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Examples of LC Connections to the EASY-Spray Column . . . . . . . . . . . . . . . . 23
Connecting the EASY-nLC 1200 to the EASY-Spray Column . . . . . . . . . . . 24
Configuring the EASY-nLC 1200 Plumbing for Two Columns . . . . . . . . . . 27
Connecting an RSLCnano System to the EASY-Spray Column. . . . . . . . . . . 27
Additional Resources. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Chapter 5 Configuring the Mass Spectrometer for NSI Mode . . . . . . . . . . . . . . . . . . . . . . . .31
Configuring the EASY-Spray NG Source’s NSI Parameters. . . . . . . . . . . . . . . . 31
Configuring the EASY-Spray Source’s NSI Parameters . . . . . . . . . . . . . . . . . . . 32
Chapter 6 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33
Guidelines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Tools and Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Cleaning the EASY-Spray Column Emitter Tip . . . . . . . . . . . . . . . . . . . . . . . . 35
Replacing the Temperature Controller PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Chapter 7 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39
Chapter 8 Replaceable Parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43
Spare Parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Consumables. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Chapter A Contents of the Installation Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47

Thermo Scientific EASY-Spray Series Ion Source User Guide vii
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Figure 1. nanoLC/MS system (example) using the EASY-Spray ion source, camera,
and monitor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Figure 2. EASY-Spray NG ion source (P/N ES082) with the USB camera . . . . . . . . . . . . . 3
Figure 3. EASY-Spray ion source (P/N ES081) with the USB camera . . . . . . . . . . . . . . . . . 4
Figure 4. Temperature controller PCB located on the EASY-Spray NG ion source
(bottom) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Figure 5. Controller power supply assembly (shown with a European input plug) . . . . . . . 6
Figure 6. Power connection for the temperature controller PCB (bottom view) . . . . . . . . . 8
Figure 7. Emitter positioning tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Figure 8. Gap positions in front of the MS ion transfer tube (side view) . . . . . . . . . . . . . . 12
Figure 9. USB camera installed on the EASY-Spray ion source . . . . . . . . . . . . . . . . . . . . 16
Figure 10. Analog camera installed on the EASY-Spray ion source . . . . . . . . . . . . . . . . . . . 17
Figure 11. EASY-Spray column . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Figure 12. nanoViper fitting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Figure 13. Recommended plumbing locations on the venting Tee (nanoViper example). . 24
Figure 14. Tee holder secured to the bottom of the column holder . . . . . . . . . . . . . . . . . . 25
Figure 15. Example of a one-column setup with nanoViper fittings. . . . . . . . . . . . . . . . . . 25
Figure 16. Example of a two-column setup with nanoViper fittings and a Viper union . . . 26
Figure 17. EASY-nLC 1200 touch screen showing a two-column configuration . . . . . . . . 27
Figure 18. Example of a one-column setup for an RSLCnano system (direct injection) . . . 28
Figure 19. Example of a two-column setup for an RSLCnano system (preconcentration) . . 29
Figure 20. Ion Source pane in the Tune window (example). . . . . . . . . . . . . . . . . . . . . . . . 32
Figure 21. NSI Source dialog box . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Figure 22. Wash cap installed over the column emitter tip . . . . . . . . . . . . . . . . . . . . . . . . 35
Figure 23. EASY-Spray ion source (bottom) with the new temperature controller PCB
and extra cover plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Figure 24. Temperature controller PCB (P/N ES233). . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Figure 25. EASY-Spray ion source (bottom) without the temperature controller PCB. . . . 37
Figures

Figures
viii EASY-Spray Series Ion Source User Guide Thermo Scientific

Thermo Scientific EASY-Spray Series Ion Source User Guide ix
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Preface
The EASY-Spray Series Ion Source User Guide describes the hardware components, and
provides installation and configuration procedures for the Thermo Scientific™ EASY-Spray™
Series ion sources. Depending on the installed camera type, you view the ion source spray by
using the camera software or an LCD monitor that is placed on the mass spectrometer (MS).
Also, because this guide uses drawings of various connections and component parts to help
illustrate procedures, be sure to start from , no matter where it appears.
Contents
• Accessing Documentation
• Providing Documentation Feedback
• Compatible Mass Spectrometers
• Special Notices, Symbols, and Cautions
• Safety Precautions
• Regulatory Compliance
•ContactingUs
Accessing Documentation
Thermo Scientific MSs include complete documentation.
•Viewing the Product Manuals
•Viewing Online User Documentation
For system requirements, refer to the release notes on the software DVD.

Preface
xEASY-Spray Series Ion Source User Guide Thermo Scientific
Viewing the Product Manuals
The Thermo Fisher Scientific service engineer installs the instrument control applications and
the instrument manuals on the data system computer.
To view the product manuals
From the Microsoft™ Windows™ taskbar, choose Start > All Apps (Windows 10) or
All Programs (Windows 7) > Thermo Instruments (or Thermo model) and so on,
where model is the specific MS.
Viewing Online User Documentation
Visit the Thermo Fisher Scientific website for product manuals and more.
To view user documentation from the Thermo Fisher Scientific website
1. Go to thermofisher.com.
2. Point to Services & Support and click Manuals on the left.
3. In the Refine Your Search box, search by the product name.
4. From the results list, click the title to open the document in your web browser, save it, or
print it.
To return to the document list, click the browser Back button.
Providing Documentation Feedback
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Complete a brief survey about this document by clicking the button below.
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