Thermo Scientific Wellwash Versa User manual

Thermo Scientific
Wellwash® Versa
User Manual
Rev. 1.0

Thermo Scientific
Wellwash®Versa
User Manual
Rev. 1.0, Cat. no. N11166

Copyright
Copyright ©2010 Thermo Fisher Scientific Corporation. All rights reserved. Printed in Finland.
Reproduction of the accompanying user documentation in whole or in part is prohibited.
Trademarks
“Nunc” and “Wellwash” are registered trademarks of Thermo Fisher Scientific Inc. and its subsidiaries.
All other trademarks and registered trademarks are the property of their respective holders.
Disclaimer
Thermo Fisher Scientific reserves the right to change its products and services at any time to incorporate
technological developments. This manual is subject to change without prior notice as part of a continuous
product development. Although this manual has been prepared with every precaution to ensure accuracy,
Thermo Fisher Scientific assumes no liability for any errors or omissions, nor for any damages resulting from
the application or use of this information. This manual supersedes all previous editions.
Remarks on screenshots
The version number displayed in screenshots may not always be the one of the currently released version.
Screenshots are only replaced if the content related to the application has changed.
No liability for consequential damages
Thermo Fisher Scientific shall not be liable for any damages whatsoever arising out of the use or inability to
use this product.
Power failure
The system requires uninterrupted power supply in order to operate correctly. Thermo Fisher Scientific has no
responsibility whatsoever for system malfunctions arising from power failures.

Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 3
About This User Manual
The Wellwash Versa microplate washer can be used as standalone in
research and routine-test laboratories by professional personnel.
This user manual is for the following instruments, Wellwash Versa:
Cat. no. 5165010 and 5165030. It has been designed to give you the
information you need for:
●Reviewing safety precautions
●Installing the instrument
●Navigating in the user interface
●Operating the instrument
●Programming of wash protocols
●Defining of wash parameters
●Performing cleaning and maintenance procedures
●Troubleshooting the instrument performance
This user manual also describes all the features and specifications of the
Wellwash Versa instrument as well as ordering information.
Read the manual in its entirety before operating the instrument.
Keep the user manual for future reference. The user manual is an
important part of the instrument and should be readily available during
use of the instrument. Keep the user manual together with the
instrument in case you distribute it onwards.
For the latest information on products and services, visit our websites
at:
http://www.thermoscientific.com
http://www.thermoscientific.com/mpi
http://www.thermoscientific.com/readingroom
http://www.thermoscientific.com/wellwash
In our efforts to provide useful and appropriate documentation, we
appreciate your comments on this user manual to your local Thermo
Fisher Scientific representative.
Intended users
How to use this
user manual
For more
information

About This User Manual
Safety symbols and markings
4Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific
These symbols are intended to draw your attention to particularly
important information and alert you to the presence of hazards as
indicated.
The following symbols and markings appear on the type label and the
instrument itself.
Power ON ▲
Power OFF ▲
Serial number ▲
Catalog number ▲
Date of manufacture ▲
Consult instructions for use ▲
WEEE symbol This product is required to comply with the European
Union’s Waste Electrical & Electronic Equipment (WEEE) Directive
2002/96/EC. ▲
A black label with the following text (Figure 2–3 on page 16):
CAUTION: WARNING: DISCONNECT SUPPLY BEFORE
SERVICING and AVERTISSEMENT: COUPER
L'ALIMENTATION AVANT L'ENTRETIEN ET LE
DEPANNAGE. ▲
Safety symbols
and markings
Safety symbols and
markings used on
the Wellwash Versa

About This User Manual
Safety symbols and markings
Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 5
The following symbols and markings appear in this user manual.
Warning Risk of electric shock. ▲
Warning Biohazard risk. ▲
Warning Risk of injury to the user(s). ▲
Caution Risk of damage to the instrument, other equipment or loss of
performance or function in a specific application. ▲
Note Marks a hint, important information that is useful in the
optimum operation of the system, or an item of interest. ▲
W
arning and other
markings used in
the documentation

About This User Manual
Safety symbols and markings
6Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific

Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 7
Contents
Intended users...................................................................................3
How to use this user manual ............................................................. 3
For more information .......................................................................3
Safety symbols and markings.............................................................4
Safety symbols and markings used on the Wellwash Versa ............. 4
Warning and other markings used in the documentation ............... 5
Introduction to the Wellwash®Versa Microplate Washer................. 13
Intended use.................................................................................... 13
Wellwash Versa Main Parts...................................................................... 15
Instrument views............................................................................. 15
Liquid system diagram ....................................................................17
USB memory stick port................................................................... 17
USB PC port................................................................................... 17
Plate carrier .....................................................................................17
Wash heads .....................................................................................17
Shaker ............................................................................................. 19
Installation .................................................................................................... 21
What to do upon delivery ...............................................................21
Unpacking the instrument ........................................................... 21
Checking delivery for completeness or damage.............................21
Environmental requirements ........................................................ 22
Installation setups............................................................................22
Releasing the transport lock.......................................................... 22
Removing the priming vessel........................................................24
Installing the wash head(s) ........................................................... 24
Liquid containers and channels ....................................................27
Connection diagram.................................................................. 28
Installing the liquid containers ..................................................28
Foaming....................................................................................30
Installing the aerosol cover ...........................................................31
Connecting the power supply cable..............................................31
Connecting to a computer............................................................... 32
Warnings and cautions.................................................................... 32
Electrical ......................................................................................32
Defects and abnormal stresses....................................................... 33
Operating precautions and limitations before operation .................. 33
Switching on ................................................................................... 34
Performing the operational check.................................................... 35
Settings after installation ................................................................. 35
Chapter 1
Chapter 2
Chapter 3

Contents
8Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific
Running Wash Protocols ............................................................................37
Filling and emptying liquid containers ............................................ 38
Selecting a protocol ......................................................................... 38
Selecting a ready-made protocol ................................................... 38
Loading the plate ............................................................................39
Priming the system..........................................................................40
Strip selection.................................................................................. 41
Strip selection with the number keys ............................................ 41
Strip selection with the Layout row .............................................. 42
Canceling a run............................................................................... 42
Rinsing the liquid system ................................................................43
Operating the Instrument............................................................................45
Display and keys for navigating and editing .................................... 45
Menus............................................................................................. 47
Main menu .....................................................................................47
Liquid level detection...................................................................48
Protocol .......................................................................................48
Creating a protocol ...................................................................49
Creating a protocol in an optional way......................................53
Adding new steps to protocols................................................... 54
Deleting steps from protocols....................................................56
Head / Plate ................................................................................. 57
Well .............................................................................................58
Microplate requirements ...........................................................59
Steps ............................................................................................60
Wash.........................................................................................60
Aspirate.....................................................................................63
Dispense ...................................................................................64
Soak .......................................................................................... 65
Prime ........................................................................................65
Pause......................................................................................... 66
Layout.......................................................................................66
Layout row................................................................................... 68
Settings menu ................................................................................. 68
Prime & rinse parameters............................................................. 68
Sensors ......................................................................................... 70
Wash head configuration..............................................................70
System.......................................................................................... 71
Reports......................................................................................... 72
Maintenance menu ......................................................................... 73
Clean ...........................................................................................73
Cleaning/Disinfection procedure............................................... 74
Clean wash head........................................................................ 76
Soak wash head .........................................................................78
Move wash head........................................................................80
Empty priming vessel ................................................................ 81
Calibration................................................................................... 82
Active calibration ......................................................................82
Chapter 4
Chapter 5

Contents
Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 9
Calibrate current wash head ...................................................... 83
Service..........................................................................................87
Calibrate dispensing pump speed ..............................................87
Exporting and Importing ............................................................................. 89
Exporting a protocol ....................................................................... 89
Importing a protocol.......................................................................90
Exporting a protocol as text file.......................................................91
Shutdown....................................................................................................... 93
Shutting down ................................................................................ 93
Emergency Situations ................................................................................. 95
Handling emergency situations ....................................................... 95
Maintenance................................................................................................. 97
Regular and preventative maintenance ............................................ 97
Maintenance checklist .................................................................. 97
Instrument care ............................................................................ 98
Cleaning the instrument.................................................................. 98
Salt deposit...................................................................................99
Cleaning the plate carrier ................................................................ 99
Cleaning liquid containers............................................................. 100
Replacing the wash head(s)............................................................ 101
Disposal of materials .....................................................................101
Decontamination procedure..........................................................102
Refitting the transport lock ........................................................... 103
Maintaining a system log...............................................................105
Packing for service.........................................................................105
Disposal of the instrument ............................................................ 105
Technical Specifications ......................................................................... 107
General specifications.................................................................... 107
Performance specifications ............................................................108
Safety specifications.......................................................................109
In conformity with the requirements.......................................... 109
Troubleshooting Guide ............................................................................. 111
Error and warning codes ...............................................................111
Troubleshooting guide ..................................................................113
Ordering Information................................................................................. 115
Wellwash Versa.............................................................................115
List of spare parts and accessories .................................................. 115
List of Thermo Scientific plates.....................................................116
System Log .................................................................................................. 117
Chapter 6
Chapter 7
Chapter 8
Chapter 9
Chapter 10
Chapter 11
Chapter 12
Appendix A

Contents
10 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific
Certificate of Decontamination ...............................................................119
Appendix B

Contents
Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 11

Contents
12 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific

Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 13
Chapter 1
Introduction to the Wellwash®
Versa Microplate Washer
The Wellwash Versa (Figure 1-1) is a microplate washer. It is designed
to use both 96- and 384-well plate formats: the 96-well plate in both
landscape and portrait orientation, and the 384-well plate in landscape
orientation. The 96-well plates and strips are designed to be washed in
1 x 8, 2 x 8, 1 x 12, or 2 x 12 format and the 384-well plates in 1 x 16
format. The instrument also allows shaking during soaking. The
instrument has an intuitive graphical user interface. In addition, the
Wellwash Versa can be connected to plate handling devices.
The Wellwash Versa can be used to wash and prepare plates for a
variety of test routines, mainly in enzyme-linked immunosorbent assay
(ELISA) tests.
The Wellwash Versa is available in the following configurations:
●Wellwash Versa 100-240V (Cat. no. 5165010)
●96- and 384-well plate washing
●Wellwash Versa 100-240V (Fisher Scientific, Cat. no. 5165030)
●96- and 384-well plate washing
Figure 1-1. Wellwash Versa microplate washer
The Wellwash Versa is a microplate washer intended for automated
washing, aspiration, dispensing and shaking of 96- and 384-well plates
and strips that meet ANSI/SBS standards. The instrument can be
Intended use

Introduction to the Wellwash® Versa Microplate Washer
Intended use
14 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific
equipped with up to four liquid containers. It can be used in research or
routine-test laboratories by professional personnel.
The Wellwash Versa is part of an analyzing system for the end user,
who is thus responsible for validation of the whole system in order to
produce reliable and safe results. If the analyzing performance is critical
for medical diagnosis, the diagnostic test result has to be checked using
internal quality controls or with a corresponding test. We recommend
using Good Laboratory Practices (GLP) during the analyzing process.
Use for self-testing is excluded.

Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 15
Chapter 2
Wellwash Versa Main Parts
This chapter describes the main parts of the Wellwash Versa
instrument.
The front view of the Wellwash Versa instrument is shown in
Figure 2–2.
Figure 2–2. Wellwash Versa front view with accessories
The back view of the Wellwash Versa instrument is shown in
Figure 2–3.
Instrument
views
Wash heads on wash head arm
Priming vessel
Keypad
Display
Plate carrier
See-through aerosol cover
Liquid containers
Plate clamp
Bottle stand (option)

Wellwash Versa Main Parts
Instrument views
16 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific
Figure 2–3. Wellwash Versa back view
The side views of the Wellwash Versa instrument are shown in
Figure 2–4.
Figure 2–4. Wellwash Versa side views
Connectors for liquid container tubes,
from left to right:
W (Waste), Rinse, B (Buffer B), and A (Buffer A)
ON/OFF switch
Power supply connector
USB port for PC
USB port for memory stick
ON/OFF switch
USB port for memory stick
Connectors for liquid
sensor cables,
from top to bottom:
W (Waste),
Rinse,
B (Buffer B), and
A (Buffer A)
Left Right
Serial RS-232 C port

Wellwash Versa Main Parts
Liquid system diagram
Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 17
Dispense valves
Dispense pump
Liquid level sensors
Liquid containers
Waste container
Wash head manifolds
Liquid valve manifold
Aspirate pump
Liquid level sensor
RBA
The instrument is equipped with a USB port (host) for an external
memory stick (Figure 2–4). You can transfer wash protocols from one
instrument to another of the same model with the USB memory stick.
The instrument is equipped with a USB port (client) for an external PC
connection. The connection is used for remote control and service of
the instrument. A separate manual, Wellwash Versa Remote Control
Command Sets (Cat. no. D08894), is available upon request.
The plate carrier of the Wellwash Versa instrument supports both
portrait and landscape orientation of the plate (Figure 2–2). A plate
clamp is incorporated into the plate carrier to keep the plate firmly
positioned during processing. A sensor in the plate clamp senses the
presence of a microplate.
The wash head alternatives (Figure 2–5) and their configurations
(Figure 2–6) are shown below.
Liquid system
diagram
USB memory
stick port
USB PC port
Plate carrier
W
ash heads

Wellwash Versa Main Parts
Wash heads
18 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific
Figure 2–5. Wellwash Versa wash head models
Figure 2–6. Configurations of Wellwash Versa wash heads