Thermo Scientific Savant Speedvac ISS110 User manual

Instruction Manual
Thermo Scientific Savant® ISS110
Speedvac®Concentrator

©2008 Thermo Fisher Scientific. All rights reserved.
“Suva®” is a registered trademark of DuPont.
All other trademarks are the property of Thermo Fisher Scientific Inc. and
its subsidiaries.

Contents
Section Introduction Page
1.0 Introduction-ISS110 1
Section Installation Page
2.0 Installation 1
Section Operation Page
3.0 Operation 1-3
3.1 Drying Rate 2
3.2 Starting and Stopping a Run 2
3.3 C amber Cover 2
3.4 Simple System Integrity Test 3
Section Application Information Page
4.0 Application Information 3
4.1 Solvent Handling 3-4
4.2 Accessories 4
4.3 Devising Protocols 4
Section Accessories Page
5.0 Accessories 5
Section TroubleS ooting Page
6.0 Troubles ooting 5
Troubles ooting Guide 7-8
Section Specifications Page
7.0 Specifications 5
Section Return Page
8.0 To Return an Instrument for Repair 6

The ISS110 SpeedVac®Concentrator is a complete system for solvent
evaporation, sample concentration, and drying using a patented technique that
combines centrifugal force, vacuum, and applied heat. It is intended for use with
aqueous and non-aggressive solvents such as ethanol and methanol. It may also
be used with small quantities of acetonitrile and ammonium hydroxide.
The ISS110 SpeedVac Concentrator combines a sample chamber, a rotor assem-
bly, an oil-free vacuum pump and a refrigerated vapor trap in a small
compact housing. The ISS110 applies vacuum to samples only when the rotor is
spinning, thus preventing bumping and foaming. Where required, the ISS110 can
apply thermal energy to the samples to counteract the cooling effect of
evaporation under vacuum.
Polypropylene fittings and Teflon®coated sample chamber and vacuum pump
heads resist corrosion and ensure reliable operation.
NPACKING. Open the shipping carton. Compare the contents with the packing
list. Inspect the system and components for any damage from shipment. If
damage is evident, save the shipping carton and immediately notify the carrier or
your distributor.
SITE PREPARATION. The ISS110 requires a stable, level surface for proper
operation. Units configured for 115 VAC, 60Hz, should be plugged into a circuit
rated for at least 10 amps. Units configured for 230 VAC, 50Hz, should be
plugged into a circuit rated for, at least, 5 amps.
IMPORTANT INFORMATION. Before operating the unit, read section 3.0
Operation and Section 4.0 Application Information to determine your specific
application requirements.
Start-of-day procedure. At the start of each day, ensure that the refrigerated
trap contains a clean, dry, Glass Condensation Flask (GCF400) and that the sup-
ply of CryoCool®Heat Transfer Fluid is adequate.
The CryoCool fluid in the refrigerated trap must be cold before drying samples.
For the best results, maintain electrical power to the system at all times to keep
the refrigerated trap cold and ready for use. When you first turn on the ISS110,
wait 45 minutes for the system to reach operating temperature before processing
samples!
GLASS CONDENSATION FLASK INSTALLATION.
1. Prepare the refrigerated stainless steel trap chamber by adding approximately
800 ml of CryoCool fluid. A line scribed on the wall of the stainless steel trap
indicates the minimum appropriate fluid level (with flask removed from trap).
CryoCool conducts heat away from the Glass Condensation Flask, allowing
vapors to condense on the flask walls.
1.0 INTROD CTION
2.0 INSTALLATION
3.0 OPERATION
1

2. Gently put a clean Glass Condensation Flask (GCF400) into the refrigerated
chamber. As you lower the flask into the chamber, the CryoCool Fluid rises.
Verify that the final CryoCool liquid level is 10 to 15 mm below the shoulder of
the flask.
3. Immediately wipe clean any CryoCool Fluid that spills onto the rubber seal.
4. Fit the white insulating Flask Seal over the glass flask to secure the flask in the
chamber. Its beveled side faces upward to admit the Flask Cap.
5. Snap the black rubber Flask Cap over the mouth of the glass flask. This pro-
vides easy tubing connection and a vacuum seal while also securing the Glass
Condensation Flask and insulating Flask Seal in the refrigerated chamber.
Rotor Installation. Open the lid of the rotor chamber. Visually align the pin on
the drive shaft with the groove on the bottom of the rotor. Carefully lower the
rotor onto the drive shaft. Rotate the rotor by hand to be sure the pin is lined up
with the groove. Secure the assembly by screwing the retaining knob into the
drive shaft above the rotor. Tighten it firmly but not excessively. oad the rotor
with samples and close the lid.
Always balance rotor loads. An unbalanced rotor causes vibration that will
damage the bearings. oad the rotor symmetrically. There need not be a tube in
each holder, but, you must be sure to evenly balance the rotor by placing an
even number of tubes opposite each other. When using a rotor with aluminum
tube holders, insert all tube holders.
By using the Drying Rate switch, thermal energy can be applied to the
concentrator chamber to counteract the cooling effect of evaporation, maintain
the samples in the liquid state, and accelerate the concentration run.
The DRYING RATE switch selects the desired drying rate:
• OW maintains the concentrator chamber at ambient temperature.
• MEDIUM maintains the concentrator chamber at approximately 43 °C.
• HIGH maintains the concentrator chamber at approximately 65 °C.
Select the drying rate according to the nature of your samples.
The CONCENTRATOR switch is normally in the OFF position. After loading and
installing the rotor, closing the lid, selecting a DRYING RATE (you may even
pre-heat the samples), press the CONCENTRATOR switch to the ON position to
start the drying run. To stop the run, press the CONCENTRATOR switch to the
OFF position. A run can be stopped at any point and restarted based on your
criterion for sample dryness.
3.1 DRYING RATE
3.2 STARTING AND STOPPING A R N
2

The chamber cover must be closed before a run can begin.
It is not possible to open the cover during a run because of the vacuum in the
concentrator chamber. In addition, a cover lock is present on 220 and 230 volt
models. The cover is locked at all times during a run and whenever power to the
unit is interrupted.
The cover lock is a safety feature that further reduces the risk of injury or damage
from the spinning rotor. Do not attempt to bypass the cover lock to conduct a
run with an open cover.
To remove samples in the event of power failure, insert, at an upward 45° angle,
the lid opening tool (provided with unit) into the vertical slot at the base of the
front of the unit. Firmly raise the handle to a horizontal position and lift up the
lid.
You can use this simple system integrity test to periodically verify the operational
integrity of the vacuum pump and refrigerated vapor trap.
1. Install a clean, dry glass condensation flask.
2. Allow the refrigerated vapor trap to operate for 45 minutes.
3. Open the chamber lid and remove the rotor.
4. Fill a plastic beaker that will fit in the chamber with 50 ml of water and place it
in the chamber. Close the lid.
5. Press the drying rate switch to the “ OW” position.
6. Start the system by pressing the concentrator switch to the “ON” position.
7. After fifteen minutes of operation press the concentrator switch to the “OFF”
position.
8. Immediately open the lid and measure the water sample temperature.
A properly functioning system will bring the sample down in temperature within
the range of 3 to 7° C (33–37° F).
The ISS110 SpeedVac Concentrator is suitable for drying or concentrating prima-
rily aqueous based samples. In addition, the small amount of ethanol
commonly dried from preparations stemming from DNA precipitations can also
be processed with this system.
3.4 SIMPLE SYSTEM INTEGRITY TEST
4.0 APPLICATION INFORMATION
3
3.3 CHAMBER COVER

Concentrating large quantities of organic solvents, such as hexane, chloroform,
dichloromethane, or acetonitrile from extraction or elution systems are best suit-
ed to the AES1010 and AES2010 SpeedVac Systems.
When processing hazardous samples, install the SCT120 Chemical Trap on the
exhaust port to capture evaporated solvent. This trap, installed in addition to the
integral refrigerated trap, provides more complete solvent trapping. The SCT120
accepts a variety of disposable cartridges to absorb volatile exhaust. Order the
DTK120R Chemical Trap Kit, which includes the trap, disposable cartridge, tubing,
and fittings. These activated-charcoal cartridges trap radioactivity. (The same
technology is commonly used in fume hood holders.)
To install a trap on the exhaust port, remove the muffler assembly from the
VAPOR EXHAUST PORT. Attach a piece of vacuum tubing to this fitting. Attach
the other end to the chemical trap. (Follow enclosed instructions supplied with
the DTK120R kit).
The chemical trap requires periodic checking to ensure its continued effective-
ness. Refer to the manual shipped with the trap.
When concentrating samples in ammonium hydroxide, install the ANT100
Ammonia Neutralizing Trap. The disposable ANS121 (Ammonia Neutralizing
Solution) bottles, which screw onto the ANT100, neutralize ammonia gas,
preventing unpleasant odors in the laboratory.
The CC120/DX Deluxe Convenience Cart is a useful accessory for the envi-
ronmental lab. The SpeedVac®resides on the top shelf, while any additional trap-
ping apparatus are placed on the bottom shelf. Addition of the cart produces a
completely mobile concentration system.
The model DVG50 Digital Vacuum Gauge can also be installed. Insertion of
the vacuum gauge tube in the vacuum tubing, on the right hand side leading to
the glass condensation trap, provides vapor pressure reading that can be gener-
ally correlated with the dryness level of the sample. Experience with your specific
test samples will determine the correlation between vacuum levels and sample
dryness.
Test runs are necessary to determine the correct time settings for a given
procedure. To obtain data that is descriptive for both concentration (reducing a
large volume to a small volume) and drying (removing all the solvent), conduct
manual test runs, using the containers, the solvent and the volumes that you will
use for actual samples. Interrupt the run every 15 minutes to measure remaining
sample volume and sample temperature. Continue this test until the test sam-
ples are completely dry. Conduct additional runs at different drying settings.
4.1 SOLVENT HANDLING
4.3 DEVISING PROTOCOLS
4
4.2 ACCESSORIES

The following drying rate table provides a guide, results will vary depending upon
test sample make up.
DTK120R Chemical Trap Kit Absorbs volatile radioactives and attaches to the
exhaust port of the instrument.
ANT100 Ammonia Neutralizing Trap Assembly Use when concentrating
samples in ammonium hydroxide. Attaches directly to the side of the instrument.
ANS121 Ammonia Neutralizing Solution Screws directly into the ANT100
assembly for neutralizing ammonia odors.
CC120/DX Deluxe Convenience Cart For easy transport of the system and
accessories the Deluxe Convenience Cart is highly recommended. The Deluxe
Cart has two corrosion resistant, fully adjustable polypropylene shelves and
swivel casters.
DVG50 Digital Vacuum Gauge Ideal for monitoring the vacuum efficiency or
sample drying efficiency. Insertion into the vacuum line between the rotor cham-
ber and the refrigerated trap provides vapor pressure readings which can be cor-
related to sample dryness level. Insertion into the vacuum line between the refrig-
erated trap and vacuum pump allows for determining pump efficiency.
See attached chart
Model Number: ISS110
Refrigerated Trap: -50°C
4 liters CFC free Refrigerant
Vacuum Pump:
Displacement: 36 /min. @ 60 Hz
Maximum Vacuum: 7 Torr
Dimensions (W x D x H) 25 in. x 26 in. x 15 in.
(62 cm x x65 cm x 37 cm)
Weight: 152 lbs.
(69 kg)
5.0 ADDITIONAL ACCESSORIES
7.0 SPECIFICATIONS
5
SO VENT DRYING RATE
OW MEDIUM HIGH
Water 1.0-2.0 2.0-3.5 3.5-5.0
6.0 TRO BLESHOOTING

Operative Power: 115VAC, 60 Hz, 10 amps
220VAC, 60 Hz, 5 amps
230 VAC, 50 Hz, 5 amps
Dependent upon ambient temperature, line voltage fluctuations, and load capaci-
ty.
In order to receive proper attention to your repair, you must first contact the
Service Department or your sales representative and receive: (1) an Return
material Authorization (RMA) number and (2) sign the Health and Safety
Clearance Form before shipping the equipment back. All items returned must be
certified to be decontaminated and free of radioactivity.
When returning equipment that may contain hazardous materials, you must pack
and label them in accordance with DOT regulations applying to the transportation
of hazardous material.Your shipping documents must also meet DOT regulations.
8.0 TO RET RN AN INSTR MENT FOR REPAIR
6