Thermo Scientific Nunc Cell Factory CF1 User manual

Thermo Scientific Nunc Cell Factory
Instructions for use: CF1, CF4, CF10 and CF40
Immediately insert
pre-sterilized air filter
into the open white
filter adaptor cap.
43 Remove seal from
one of the white filter
adaptor caps.
2Accessories:
Air filter, connector,
white filter adaptor
cap.
Unpack the Nunc™
Cell Factory and place
it in a laminar air
flow cabinet in order
to work in sterile
conditions.
1
Prepare cell
suspension in an
aspirator bottle, mount
with sterile connector,
and clamp.
5Remove second white
filter adaptor cap from
the Cell Factory.
6
Turn Cell Factory on its
side. Raise aspirator
bottle above Cell
Factory level.
8
Insert connector.
7

Gently agitate aspirator
bottle. Loosen clamp
and cell suspension
will flow into the Cell
Factory. Chambers will
initially fill unevenly
due to a difference in
pressure.
910 When filling is
complete, allow
levels of liquid in
all chambers to
equalize through the
connecting channel.
The surface of all the
trays will be covered
by medium. Remove
the connector, replace
the white filter adaptor
cap, but leave the filter
on and incubate.
13 14 To empty the Cell
Factory place it above
aspirator bottle, in the
position shown, and
the liquid will run out.
Turn the Cell Factory
90º, so that filling inlet
is up. Medium will
be separated with
equal amounts in each
chamber.
11 12 Place the Cell Factory
in horizontal incubation
position.
Tilt the Cell Factory to
drain it completely.
15 16 To decontaminate the
Cell Factory after use
it is recommended to
autoclave: 60 min,
132°C.
© 2010 Thermo Fisher Scientific Inc. All Rights Reserved.
All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.
MNLSPCF10INSTR 0210
47201 Ver. 6.0
Asia: China Toll-free: 800-810-5118 or 400-650-5118; India: [phone], India Toll-free: 1 800 22 8374; Japan: [phone]
Other Asian countries: 65 68729717
Europe: Austria: [phone]; Belgium: [phone]; Denmark: [phone]; France: [phone]; Germany: [phone]
6940, Germany Toll-free: 08001-536 376; Italy: [phone] or 434-254-375;
Netherlands: [phone]; Nordic/Baltic countries: [phone]; Russia/CIS: [phone];
Spain/Portugal: [phone]; Switzerland: [phone]; UK/Ireland: [phone]
North America: USA/Canada [phone]; USA Toll-free: [phone]
South America: USA sales support: [phone]
Countries not listed: [phone] or [phone]
www.thermoscientific.com/bioproduction
Thermo Scientific Nunc Cell Factory
Instructions for use: CF1 CF4, CF10 and CF40