Thermo Scientific Nicolet Summit User manual

Nicolet Summit
FTIRSpectrometer
USER GUIDE
269-345500 B Revision A October 2020
© 2020 Thermo Fisher Scientific Inc. All rights reserved.

For technical support, please contact: www.thermofisher.com
Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to
use in the product operation. This document is copyright protected and any reproduction of the
whole or any part of this document is strictly prohibited, except with the written authorization of
Thermo Fisher Scientific Inc.
The contents of this document are subject to change without notice. All technical information in
this document is for reference purposes only. System configurations and specifications in this
document supersede all previous information received by the purchaser.
Thermo Fisher Scientific Inc. makes no representations that this document is complete,
accurate or error-free and assumes no responsibility and will not be liable for any errors,
omissions, damage or loss that might result from any use of this document, even if the
information in the document is followed properly.
This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a
purchaser. This document shall in no way govern or modify any Terms and Conditions of Sale,
which Terms and Conditions of Sale shall govern all conflicting information between the two
documents.
For Research Use Only. This instrument or accessory is not a medical device and is not
intended to be used for the prevention, diagnosis, treatment or cure of disease.
WARNING
Avoid an explosion or fire hazard.
This instrument or accessory is not designed for use in an explosive
atmosphere.

Contents
Welcome 1
Conventions Used 2
Ordering Parts 3
Contacting Us 3
Get Started with Your Nicolet Summit Spectrometer 5
Before Your Summit Spectrometer Arrives 6
Personal Safety with the Summit Spectrometer 9
Unpack and Get Started with the Spectrometer 15
Install a Sampling Accessory 19
Understand the Spectrometer LightBar 25
View Your Data with the OMNIC Anywhere App 28
Options and Accessories 29
Sampling Accessories for the Summit Spectrometer 30
Install and Maintain a Purge Kit 33
Maintenance 41
Maintenance Schedule for the Summit Spectrometer 42
Align the Spectrometer Optics 45
Calibrate the Laser Frequency 46
Clean the Spectrometer 47
Replace the Desiccant 48
Replace the Source 53
Replace the Sample Compartment Windows 57

Welcome
The Thermo Scientific Nicolet Summit Fourier transform infrared (FTIR) spectrometer lets you perform
chemical analyses of sample materials by collecting data in the mid-IR spectral range using a variety of
accessories. The system has integrated verification features, a powerful software suite, and many other
features that make data collection easy. You can install optional hardware and perform several service
and maintenance procedures yourself. This document, or other provided documentation, contains the
information you need.
Be sure to read the safety guide that came with the system before operating the instrument.
Note We recommend that you maintain seal and desiccation and/or purge your instrument at all
times. Equipment damage due to failure to maintain seal and desiccation and/or purge is not covered
by the warranty. If you have questions about this requirement, please contact us.
Thermo Scientific Nicolet Summit LITE FTIR Spectrometer User Guide 1
Chapter 1

Welcome
Conventions Used
Safety precautions and other important information use the following format:
DANGER
Avoid hazard. Indicates a hazardous situation which, if not avoided, will
result in serious injury or death.
WARNING
Avoid hazard. Indicates a hazardous situation which, if not avoided,
could result in serious injury or death.
CAUTION
Avoid hazard. Indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury.
NOTICE
Follow instructions with this label to avoid damaging the system hardware or losing data.
Note Contains helpful supplementary information.
2 Nicolet Summit LITE FTIR Spectrometer User Guide Thermo Scientific

Ordering Parts
To order parts, contact us.
If you need to send the instrument or an accessory to us for repair, call or e-mail us first for any shipping
requirements or other instructions.
Contacting Us
For Technical Support, please contact: www.thermofisher.com
Thermo Scientific Nicolet Summit LITE FTIR Spectrometer User Guide 3
Welcome

[This page intentionally left blank]

Get Started with Your Nicolet
Summit Spectrometer
To get started with your new Nicolet Summit Spectrometer, review the site and safety requirements
before the instrument arrives, unpack and setup the instrument, and connect any additional accessories.
Before Your Summit Spectrometer Arrives 6
Personal Safety with the Summit Spectrometer 9
Unpack and Get Started with the Spectrometer 15
Install a Sampling Accessory 19
Understand the Spectrometer LightBar 25
View Your Data with the OMNIC Anywhere App 28
Thermo Scientific Nicolet Summit LITE FTIR Spectrometer User Guide 5
Chapter 2

Get Started with Your Nicolet Summit Spectrometer
Before Your Summit Spectrometer Arrives
Prepare a suitable workspace for your spectrometer before it arrives to ensure the most accurate data
and the best long-term performance.
This article explains what to do when your spectrometer arrives and introduces environmental and
electrical factors that can influence your spectrometer’s performance. For a more comprehensive
explanation of these requirements, see the Site and Safety Information for the spectrometer.
Unpacking the Spectrometer
Before you open the box, there are two important steps to take as soon as the instrument arrives:
lCheck the exterior of the shipping box for damage
If you notice any signs of damage, contact us or your local distributor for instructions.
lAllow the spectrometer to come to room temperature
Inside the shipping box, the spectrometer is sealed in a plastic bag to keep it dry. You must allow 24
hours for the spectrometer to reach room temperature before you open the bag. If you open the
bag before the spectrometer is warmed up, condensation could form that could damage internal
optical components and cause permanent damage.
The warranty will not cover damage due to improper moving techniques or removing the sealed plastic
bag before the instrument has come to room temperature.
Preparing the Workspace
Before your instrument arrives, make sure your workspace will be able to properly support the
spectrometer. In addition to allowing enough room for the spectrometer, consider several environmental
and electrical requirements.
Spectrometer Dimensions
The Summit spectrometer requires a fairly small space, but make sure you also leave room around the
instrument so that heat can dissipate from the vents and so that you can easily access the instrument’s
ports, power switch, and cables.
lSummit instrument weight: 9.6 Kg (21 lbs)
lDimensions (W x H x D): 34 cm x 24 cm x 32 cm; (13.3 in. x 9.6 in. x 12.7 in.)
6 Nicolet Summit LITE FTIR Spectrometer User Guide Thermo Scientific

Environmental Factors
The Summit spectrometer is a robust instrument designed to be used in many environments. However,
for best performance, keep it in a relatively dust-free and low-humidity environment. The spectrometer
operates reliably at temperatures between 15 and 35 °C, but for optimal performance should be kept
between 20 and 22 °C.
Humidity can cause condensation to form inside the instrument, which could damage internal
components. A few precautions can typically protect your instrument from humidity:
lMaintain the instrument’s desiccant, including while the instrument is in storage.
lAvoid rapid changes in temperature.
nKeep the instrument away from sources of cool or hot air, such as near heating and air con-
ditioning vents or large windows.
If your spectrometer will be kept in an especially humid environment, consider installing a purge gas kit.
Electrical Requirements
Power supplied to the spectrometer must be from dedicated, uninterrupted sources and free from the
following issues:
lVoltage dropouts
lTransient spikes
lFrequency shifts
lOther line disturbances
If you suspect problems with your power, we recommend a power quality audit. Contact us or your local
electrical authority for more information.
Electrical Service Specifications
The following table lists specifications for electrical service. Contact our service representative in your
area if you have questions about the requirements.
Requirements Specification
Input Current 1.5 A Max
Thermo Scientific Nicolet Summit LITE FTIR Spectrometer User Guide 7
Get Started with Your Nicolet Summit Spectrometer

Get Started with Your Nicolet Summit Spectrometer
Requirements Specification
Input Voltage 100 to 240 VAC
Line Frequency 50 to 60
Line
Disturbances
Sags, surges, or other line disturbances must not exceed 10% of input
voltage
Noise Less than 2 V (common mode)
Less than 20 V (normal mode)
8 Nicolet Summit LITE FTIR Spectrometer User Guide Thermo Scientific

Personal Safety with the Summit
Spectrometer
While the Nicolet Summit Spectrometer is designed to be a safe instrument, you should take a few
precautions to protect yourself from potential hazards that can arise during normal use and
maintenance.
CAUTION
This guide is an introduction to potential dangers that you should be
aware of, but it is not a comprehensive guide. Before using the instru-
ment, see the Site and Safety Information for the spectrometer for a full
description of these potential hazards.
Potential Hazards during Normal Use
During normal use, most hazards are due to following sources:
lPotentially hazardous samples and solvents
lParts of the spectrometer that are hot or emit heat
lThe instrument’s laser
You can avoid possibly harming yourself or damaging the instrument by understanding potential
hazards and by taking a few precautions.
Hazardous Samples and Solvents
Take special precautions if you are using or plan to measure potentially hazardous samples or solvents,
such as pressurized gases or corrosive or flammable solvents.
USE PROPER VENTILATION
There are no special ventilation requirements for your spectrometer, but you may need additional
ventilation during certain types of analysis. Ensure that you have proper ventilation if you will be
analyzing highly toxic samples, dissolving your samples in solvents that may interact with the infrared
source, or sampling flammable gases.
The pyrolysis of solvents containing halogenated hydrocarbons may produce hydrochloric acid (HCl),
hydroflouric acic (HF), or phosgene (COCl2).
Thermo Scientific Nicolet Summit LITE FTIR Spectrometer User Guide 9
Get Started with Your Nicolet Summit Spectrometer

Get Started with Your Nicolet Summit Spectrometer
WARNING
Avoid toxic inhalation. Hydrochloric acid, hydrofluoric acid, and phos-
gene are highly toxic. If you are using solvents containing halogenated
hydrocarbons, ensure your work area is properly ventilated.
VOLATILE AND FLAMMABLE SOLVENTS
The infrared source inside the spectrometer can ignite flammable and volatile samples and solvents.
Take the following actions when working with samples and solvents that are flammable:
lWork with the sample compartment windows installed.
lEnsure that the workspace is properly ventilated with an active venting system that is free of spark or
other sources of ignition and that prevents flammable vapors from collecting in the atmosphere sur-
rounding the instrument.
lDo not leave flammable solvents or samples near the instrument.
lDo not leave flammable solvents or samples in the sample compartment for longer than necessary.
lPurge the spectrometer with clean, dry air or nitrogen.
CORROSIVE SOLVENTS
Solvents that produce HCl or HF vapors in the sample compartment may severely damage the system.
If you are using halogenated solvents, purge the instrument with clean, dry air or nitrogen.
NOTICE
Your warranty does not cover equipment damage that is due to a failure to purge the instrument.
HCl and HF vapors may also compromise the coating of KBr sample compartment windows. If you are
planning to work with corrosive solvents regularly, consider installing ZnSe sample compartment
windows, instead.
BIOHAZARD OR RADIOACTIVE MATERIALS AND INFECTIOUS AGENTS
Biological samples, such as tissues, body fluids, infectious agents, and blood, have the potential to
transmit infectious diseases. Follow your organization’s Biosafety Program protocols for working with
potentially infectious materials.
10 Nicolet Summit LITE FTIR Spectrometer User Guide Thermo Scientific

Sources of Heat
Parts of the spectrometer can become quite hot during normal use. Be careful around the
spectrometer’s infrared source and the vents.
Figure 2-1: Take care around the instrument’s infrared source and the vents
The outward facing surface of the infrared source is located on the bottom of the instrument and can
become quite hot. Do not touch the underside of the instrument during operation or shortly after use.
The spectrometer’s vent is located on the left side of the instrument. During normal use, the
spectrometer dissipates hot air from internal components to the area outside the spectrometer through
these vents. Leave enough space around the instrument vents for hot air to dissipate.
Laser and Optical Safety
You will never be exposed to unsafe levels of laser radiation during normal use of the spectrometer. If
the cover is removed during a service procedure, you may need to take special precautions, such as
using protective eyewear. Your service person will notify you if this is necessary.
WARNING
Avoid personal injury.
Never stare into the laser beam or at its reflection. Never tamper with the laser,
even if you are replacing a defective laser, or you could be exposed to laser light or
to high voltage.
Thermo Scientific Nicolet Summit LITE FTIR Spectrometer User Guide 11
Get Started with Your Nicolet Summit Spectrometer

Get Started with Your Nicolet Summit Spectrometer
Potential Hazards during Maintenance
You may be exposed to different hazards while performing maintenance on the instrument than you
would encounter during normal use. During maintenance, the primary hazards involve purging the
instrument and working with the instrument’s internal components.
Purging the Instrument
In especially humid environments, we recommend installing a source of clean, dry air or nitrogen to
purge the spectrometer. Purging the instrument can help protect internal optical components from
damage caused by a moist environment or corrosive solvents, and it can ensure more accurate results.
See "Install and Maintain a Purge Kit" for details on purchasing and installing a purge kit for your
spectrometer.
DANGER
Avoid fire and explosion hazard.
lUse only dried air or nitrogen to purge your spectrometer.
lNever use a flammable, combustible, or toxic gas to purge this instrument. The
purge gas must be free of oil and other reactive materials. Heat from the source
or from laser absorption may ignite flammable gases or reactive materials in
the purge gas.
Working with Internal Components
Typically, there should be no reason for you to remove the instrument cover or attempt to replace
internal components. However, if you need to remove the cover for maintenance, be aware that you risk
being shocked, burned, and exposed to laser light.
CAUTION
Avoid shock hazard.
Even after this instrument has been disconnected from all voltage sources,
capacitors may remain charged for up to 30 seconds and can cause an electrical
shock.
12 Nicolet Summit LITE FTIR Spectrometer User Guide Thermo Scientific

CAUTION
Avoid burn hazard.
Internal components, especially the infrared source, can become extremely hot
during normal operation. Turn off the instrument and wait at least 10 minutes
before replacing any components.
WARNING
Avoid personal injury.
lNever stare into the laser beam or at its reflection. Never tamper with the laser.
You could be exposed to laser light or high voltage.
lIf you adjust the laser or perform procedures that are not described in the user
guides and manuals, you could be exposed to hazardous radiation.
REPLACING THE DESICCANT
When you open the desiccant compartment, you must prevent flammable liquids or gases from entering
the compartment. For instructions on changing the desiccant, see "Replace the Desiccant".
DANGER
Avoid explosion hazard.
Before you open the desiccant compartment, turn off the instrument power, unplug
the power cord and remove all accessories and samples from the system. Entry of
flammable liquids or gases into the desiccant compartment could cause an
explosion. If such entry occurs, contact us immediately and do not apply power to
the instrument until the condition has been corrected.
REPLACING THE IR SOURCE
The IR source becomes extremely hot during use. If you need to replace the IR source, avoid burn and
explosion hazards.
CAUTION
Avoid burn hazard.
The source becomes extremely hot during normal use. Always allow the source to
cool for at least 10 minutes after the spectrometer is turned off before you work
with the source.
Thermo Scientific Nicolet Summit LITE FTIR Spectrometer User Guide 13
Get Started with Your Nicolet Summit Spectrometer

Get Started with Your Nicolet Summit Spectrometer
DANGER
Avoid explosion hazard.
Before you remove the source from the spectrometer, turn off the instrument
power, unplug the power cord, disconnect any purge lines, and remove all
accessories and samples from the system. Entry of flammable liquids or gases into
the source compartment could cause an explosion. If such entry occurs, contact us
immediately and do not apply power to the instrument until the condition has been
corrected.
Summary
The Nicolet Summit spectrometer is a safe and rugged instrument, but you can be exposed to hazards
during use and maintenance. During normal operation, use caution when handling potentially
hazardous samples and solvents and avoid parts of the spectrometer that are hot or emit heat. During
maintenance, take precautions to avoid harm or damage that could occur when purging the instrument,
handling internal components, or changing the desiccant.
14 Nicolet Summit LITE FTIR Spectrometer User Guide Thermo Scientific

Unpack and Get Started with the
Spectrometer
Unpacking and setting up your Thermo Scientific Nicolet Summit Spectrometer requires you to place the
instrument in your workspace, to connect and power on the spectrometer, and to run a short
performance test.
Before You Begin
NOTICE
Wait 24 hours before taking the spectrometer out of its plastic shipping bag to prevent condensation
from forming that can damage internal optics. The warranty will not cover damage due to opening the
sealed plastic bag before the instrument has come to room temperature.
Reminder: Review the spectrometer's Site and Safety Information to ensure that the workspace is safe
and suitable for the instrument.
Unpacking and Setup
The steps for unpacking and setting up your spectrometer vary somewhat depending on the options you
purchased.
1. Unpack the shipping box
Remove the spectrometer from the shipping box and bag and remove additional packing materials.
NOTICE
Use care when lifting the spectrometer.
lDo not touch the sample compartment windows while unpacking the instrument. Touching the
windows could cause permanent damage.
Thermo Scientific Nicolet Summit LITE FTIR Spectrometer User Guide 15
Get Started with Your Nicolet Summit Spectrometer

Get Started with Your Nicolet Summit Spectrometer
NOTICE
2. (Optional) Replace the sample compartment windows.
If you purchased the ZnSe windows option, replace the windows. For instructions on replacing the
windows, see "Replace the Sample Compartment Windows".
3. Turn on the spectrometer.
a. Connect the power supply to the spectrometer and to a properly grounded AC power source.
b. Press the instrument's power switch to turn on the spectrometer. The spectrometer's LightBar
displays a cycling green light while the instrument warms up and shows a solid green bar when
the instrument is ready for use.
4. Install OMNICParadigm software.
a. Insert the OMNICParadigm software DVD.
b. If the DVDdoes not start automatically, open the DVDfiles and click Start to begin the
installation. Follow the on-screen prompts to install OMNICParadigm software.
The software installation may take up to 20 minutes to complete. You will need to restart the
computer to complete the installation.
5. Connect the spectrometer
a. Use the USB Type A to Type B cable to connect the spectrometer to your computer. The
spectrometer has a USB Type B receptacle.
16 Nicolet Summit LITE FTIR Spectrometer User Guide Thermo Scientific

b. Open OMNICParadigm software. The first time you open the application, it opens in
Workstation mode.
c. After you connect the spectrometer, OMNICParadigm software recognizes the spectrometer
automatically and displays the instrument type in the upper right corner of the screen. If the
instrument is not connected automatically, you may have to connect manually.
To connect manually, go to Configure >Connectivity and select the Summit LITEfrom the
connectivity dialog.
6. Verify the spectrometer’s performance.
Before taking the following steps, make sure there is not a sampling accessory in the sampling
compartment.
a. Align the spectrometer optics.
i. From the dashboard of OMNICParadigm software, go to Acquire Data >Diagnostics
>Align Spectrometer.
ii. Select Start to begin alignment. The screen says "Align complete"when alignment has fin-
ished successfully.
b. Calibrate the laser.
i. Go to Acquire Data >Diagnostics >Laser Calibration.
ii. Select Start to begin calibration.
Laser calibration may take several minutes to complete. When calibration is finished, the
display shows "Measurement complete" and indicates any change in laser frequency.
The instrument takes up to 12 hours to come to thermal equilibrium. For best results, re-run
alignment and calibration after the first 12 hours of operation.
c. Run thet Nicolet FTIR – Factory Qualification workflow. Performance verification and
qualification workflows use a polystyrene reference material inside the spectrometer to run a
Thermo Scientific Nicolet Summit LITE FTIR Spectrometer User Guide 17
Get Started with Your Nicolet Summit Spectrometer