Thermo Scientific Finnpipette F2 User manual

1
Finnpipette®F2
Single Channel
Variable & Fixed Volume
Multichannel
Instructions for Use
操作指南
User Manual

2
This product complies with the European Union Directive 98/79/EC, and it is marked with
a CE-marking.
When the product is used in applications related to the directive 98/79/EC, read the
additional information at www.thermofisher.com or contact the manufacturer at
info.pipettes@thermofisher.com to ensure correct and safe use.
The CE-mark covers the system containing the CE-marked Finnpipette F2 pipette and the
CE-marked Finntips.
Product specifications are subject to change without prior notice. Finnpipette®and Finntip®
are registered trademarks of Thermo Fisher Scientific Oy.
本产品符合欧盟指令98/79/EC的标准，并标有CE标志。
如须依据该指令使用本移液器，使用者还应阅读 www.thermofisher.com 上的
其它有关信息，或者通过 [email protected] 邮箱联系制造商以
确保正确安全地使用。
CE 标志涵盖了包括印有CE标志的Finnpipette F2以及Finntips的整个体系。
产品规格可能有变，恕不另行通知。
Finnpipette® 和Finntip® 均为Thermo Fisher Scientific的注册商标。

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CONTENTS
PRODUCT DESCRIPTION 4
PACKAGE 5
SAFETY 5
PIPETTE OPERATION 6
PIPETTING TECHNIQUES 6
CALIBRATION AND ADJUSTMENT 8
MAINTENANCE 12
TROUBLE SHOOTING 14
SPARE PARTS 27-36
目录
产品简介 15
包装 16
安全 16
移液器的操作 17
移液方法 17
校准与调节 19
维护保养 23
故障检修 25
备件 27-36

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Product description
The Finnpipette F2 is a continuously adjustable, general purpose micropipette for sampling and
dispensing accurate liquid volumes.
It operates on an air displacement principle (i.e. an air interface) and uses detachable, disposable
tips.
The adjusted delivery volume is displayed digitally on a readout window in the handle.
The thirteen different models of Finnpipette F2 pipettes cover a volume range from 0,2 µl to 10 ml.
Order No. Volume Range Color code Finntip
4642010 0,2 µl to 2 µl pink Flex 10, 10, 20, 50
4642020 0,5 µl to 5 µl pink Flex 10, 10, 20, 50
4642030 1 µl to 10 µl pink Flex 10, 10, 20, 50
4642040 1 µl to 10 µl yellow
Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4642050 2 µl to 20 µl turquoise 50
4642060 2 µl to 20 µl yellow Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4642120 5 µl to 50 µl turquoise 50
4642130 5 µl to 50 µl yellow Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4642070 10 µl to 100 µl yellow Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4642080 20 µl to 200 µl yellow Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4642090 100 µl to 1000 µl blue Flex 1000, 1000, 1000 Ext
4642100 0,5 ml to 5 ml green 5 ml
4642110 1 ml to 10 ml red 10 ml, Flex 10 ml Ext
The fifteen different models of Finnpipette F2 Fixed Volume pipettes cover a volume range from
1 µl to 10 ml.
Order No. Volume Range Color code Finntip
4652000 1 µl dark blue Flex 10, 10, 20, 50
4652010 5 µl dark blue Flex 10, 10, 20, 50
4652020 10 µl dark blue Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4652130 20 µl dark blue Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4652030 25 µl dark blue Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4652040 50 µl dark blue Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4652050 100 µl dark blue Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4652140 200 µl dark blue Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4652060 250 µl dark blue Flex 1000, 1000, 1000 Ext
4652070 500 µl dark blue Flex 1000, 1000, 1000 Ext
4652080 1000 µl dark blue Flex 1000, 1000, 1000 Ext
4652090 2000 µl dark blue 5 ml
4652100 3000 µl dark blue 5 ml
4652110 5000 µl dark blue 5 ml
4652120 10000 µl dark blue 10 ml, Flex 10 ml Ext
The ten different models of Finnpipette F2 Multichannel pipettes cover a volume range from
1 µl to 300 µl.
Order No. Channel Volume Range Color code Finntip
4662000 8 1 µl to 10 µl pink Flex 10, 10, 20, 50
4662010 8 5 µl to 50 µl yellow Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4662020 8 10 µl to 100 µl yellow Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4662030 8 30 µl to 300 µl orange Flex 300, 300
4662040 12 1 µl to 10 µl pink Flex 10, 10, 20, 50
4662050 12 5 µl to 50 µl yellow Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4662060 12 10 µl to 100 µl yellow Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4662070 12 30 µl to 300 µl orange Flex 300, 300
4662080 16 1 µl to 10 µl purple 20
4662090 16 5 µl to 50 µl turquoise 50

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Digital display
The adjusted delivery volume is clearly indicated in the large
digital display on the handle.
Raw materials
The Finnpipette F2 is made of mechanically durable and chemically resistant materials.
Description of tips
Finntips are recommended for use with the Finnpipette F2.
They are made of virgin natural colour polypropylene, generally regarded as the only
contamination free material suitable for tips. Finntips are also autoclavable (121°C).
Package
The Finnpipette F2 is shipped in a specially designed package containing the following items:
1. The Finnpipette
2. Service tool
3. Multichannel service tool
4. Bag of grease 1g (Order No. 3300200)
5. Instructions for Use
6. Calibration certificate / Warranty certificate
Safety
Cautions are marked with this symbol .
Intended use
The intended use of the device is to transfer liquids in the volume range of 1 µl to 1000 µl.
The F2 Finnpipette pipette and Finntips are designed as a component of an analyzing system
for an end user, who is responsible for validating the system to ensure reliable and safe results.
Damage to health
Follow general procedures for hazard prevention and safety instructions; e.g. wear
protective clothing, eye protection and gloves.
For use and waste disposal of hazardous (e.g. radioactive and potentially infectious)
material, follow the safety instructions and general laboratory practice.
The pipette and tips are not intended for in vivo use. Do not use the pipette for pipetting any
liquid to be injected into a human body.
Do not eject the tip towards anybody.
The Finnpipette F2 is to be used by trained personnel with required laboratory skills. The
instructions for use must be read prior to and during the use of the device (pipette and tip). The
F2 pipette can be used between +4°C and +40°C.
Incorrect dispensing results
Performance may vary due to:
a. pipetting method (forward pipetting technique recommended)
b. temperature (air, liquid, vessel, pipette, and tip)
c. pressure
d. humidity
e. operator, e.g. thumb movement, pipetting angle
f. liquid density, viscosity and vapor pressure
g. type of tip
If the pipetting performance is critical to the outcome of a specific application, the result has
to be assured with an alternative test, and if this is not an option, by duplicate testing.
The possibility of an incorrect volume delivery during pipetting cannot be entirely mitigated.

6
To avoid inaccurate dispensing and/or leakage, check that the tip is properly attached to
the pipette.
When rotating the volume adjustment button, do not exceed the volume range of the pipette.
Otherwise the pipette may be damaged or affect pipetting performance.
Pipetting performance cannot be guaranteed if the tip is reused.
Choose only a tip and pipette with matching color coding.
Disposal of the Materials
Follow laboratory and country-specifi c procedures for the disposal of biohazardous or radioactive
waste. Refer to local regulations for the disposal of infectious material.
Warning The tips can be potentially infectious. Dispose of all used disposable tips as
biohazardous waste.
Warning Decontaminate the pipette before disposal.
Waste disposal according to local instructions taking into account legislation in place. For more
information, please contact us at info.pipettes@thermofisher.com or your local Thermo
Fisher Scientific representative.
Regarding the original packaging and packing materials, use the recycling operators known
to you.
Pipette operation
Setting the delivery volume
1. Set the delivery volume using the push button on the top of
the pipette. To increase the delivery volume, turn the push
button counterclockwise. To decrease the delivery volume,
turn it clockwise.
2. Make sure that the desired delivery volume clicks into place.
3. Do not set volumes outside the pipette´s specified volume range.
Using excessive force to turn the push button outside the range
may jam the mechanism and eventually damage the pipette.
Safety Label
You can mark the pipette application, your initials, the
calibration date, etc. on the safety label. Remove the
old label with a sharp needle. Mark the new label with a
pencil and slide the label back in place.
Tip ejection
To help eliminate the risk of contamination, each pipette is
fitted with a tip ejector system. To release the tip, point
the pipette at suitable waste receptacle and press the tip
ejector with your thumb.
Pipetting techniques
Push and release the push button slowly at all times particularly when working with
high viscosity liquids. Never allow the push button to snap back.
Make sure that the tip is firmly attached to the tip cone. Check for foreign
particles in the tip.
Before you begin your actual pipetting work, fill and empty the tip 2-3 times with
the solution that you will be pipetting. Hold the pipette in an upright position while
aspirating liquid. The grippy should rest on your index finger. Make sure that the tips,
pipette and solution are at the same temperature.
+

7
Forward technique
Fill a clean reagent reservoir with the liquid to be dispensed.
1 Depress the push button to the first stop.
2. Dip the tip under the surface of the liquid in the
reservoir to a depth of about 1 cm and slowly
release the push button. Withdraw the tip from
the liquid touching it against the edge of the
reservoir to remove excess liquid.
3. Deliver the liquid by gently depressing the push
button to the first stop. After a delay of about
one second, continue to depress the push button
all the way to the second stop. This action will empty the tip.
4. Release the push button to the ready position. If necessary, change the tip and continue
pipetting.
Reverse technique
The reverse technique is suitable for dispensing liquids that have a high viscosity or a tendency
to foam easily. The technique is also recommended for dispensing very small volumes. Fill a
clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button all the way to the second stop.
2. Dip the tip under the surface of the liquid in the
reservoir to a depth of about 1 cm, and slowly
release the push button. This action will fill the
tip. Withdraw the tip from the liquid touching
it against the edge of the reservoir to remove
excess liquid.
3. Deliver the preset volume by gently depressing
the push button to the first stop. Hold the push
button at the first stop. Some liquid will remain
in the tip and this should not be included in
the delivery.
4. The remaining liquid should either be discarded with the tip or pipetted back into the
container.
Repetitive technique
The repetitive technique offers a rapid and simple procedure for repeated delivery of the same
volume. Fill a clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button all the way to the second stop.
2. Dip the tip under the surface of the liquid in the
reservoir to a depth of about 1 cm, and slowly re
lease the push button. This action will fill the tip.
Withdraw the tipfrom the liquid touching against
the edge of the reservoir to remove excess liquid.
3. Deliver the preset volume by gently depressing
the push button to the first stop. Hold the push
button at the first stop. Some liquid will remain
in the tip and this should not be included in
the delivery.
4. Continue pipetting by repeating steps 3 and 4.
Pipetting of heterogeneous samples
(deproteinization in blood glucose determination, for example)
Use steps 1 and 2 of the forward technique to fill the tip with blood.
Wipe the tip carefully with a dry clean tissue.
1 2 3 4
1 2 3 4
1 2 3 4

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1. Immerse the tip into the reagent and depress the
push button to the first stop, making sure the tip
is well below the surface.
2. Release the push button slowly to the ready
position.
This will fill the tip. Keep the tip in the solution.
3. Depress the push button to the first stop and
release slowly. Keep repeating this procedure
until the interior wall of the tip is clear.
4. Finally, depress the push button all the way to the
second stop to completely empty the tip.
Calibration and adjustment
All Finnpipettes are factory calibrated and adjusted to give the volumes as specified with
distilled or deionized water using the forward pipetting technique. It should be noted that the
use of other pipetting techniques may affect the calibration results. The pipettes are constructed
to permit re-adjustment for other pipetting techniques or liquids of different temperature and
viscosity.
Device requirements and test conditions
An analytical balance must be used. The scale graduation value of the balance should be chosen
according to the selected test volume of the pipette:
Volume range readable graduation
under 10 µl 0.00 1 mg
10-100 µl 0.01 mg
above 100 µl 0.1 mg
Test liquid: Water, distilled or deionized, “grade 3” water conforming ISO 3696. Tests are done
in a draft-free room at a constant (±0.5°C) temperature of water, pipette and air between 15°C
to 30°C. The relative humidity must be above 50%. Especially with volumes under 50 µl the
air humidity should be as high as possible to reduce the effect of evaporation loss. Special
accessories, such as the evaporation trap, are recommended.
Procedure to check calibration
The pipette is checked with the maximum volume (nominal volume) and with the minimum
volume. A new tip is first pre-wetted 3-5 times and a series of ten pipettings is done with
both volumes. A pipette is always adjusted for delivery (Ex) of the selected volume. Use of
forward pipetting technique is recommended. The maximum permissible errors are designed for
forward method. It is recommended to calibrate all channels of the multichannel pipette, but a
suitable method is also to calibrate only the edge channels. Adjustment check prior calibration is
done using one of the middle channels. It is recommended to use the ISO8655 calibration limits
presented in the following tables or user-defined specifications.
Procedure:
1. Do 10 pipettings with the minimum volume.
2. Do 10 pipettings with the maximum volume.
3. Calculate the inaccuracy (A) and imprecision (cv) of both series.
4. Compare the results to the limits.
If the calculated results are within the selected limits, the adjustment of the pipette is correct.
1 2 3 4 5

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ISO8655 calibration specifications
Thermo Scientific Finnpipette F2 specifications according to ISO8655 standard.
Finnpipette F2 Variable Volume Single Channel
Range Volume Inaccuracy Imprecision
µl µl % s.d. µl cv%
0,2-2 µl 2 ±0.080 ±4 0.040 2.0
0.2 ±0.080 ±40 0.040 20.0
0,5-5 µl 5 ±0.125 ±2.5 0.075 1.5
0.5 ±0.125 ±25 0.075 15
1-10 µl 10 ±0.120 ±1.2 0.080 0.8
1 ±0.120 ±12 0.080 8.0
2-20 µl 20 ±0.20 ±1.0 0.10 0.5
2 ±0.20 ±10.0 0.10 5.0
5-50 µl 50 ±0.50 ±1.0 0.20 0.4
5 ±0.50 ±10 0.20 4.0
10-100 µl 100 ±0.80 ±0.8 0.30 0.3
10 ±0.80 ±8.0 0.30 3.0
20-200 µl 200 ±1.60 ±0.8 0.60 0.3
20 ±1.60 ±8.0 0.60 3.0
100-1000 µl 1000 ±8.0 ±0.8 3.0 0.3
100 ±8.0 ±8.0 3.0 3.0
0,5-5 ml 5000 ±40.0 ±0.8 15.0 0.3
500 ±40.0 ±8.0 15.0 3.0
1-10 ml 10000 ±60.0 ±0.6 30.0 0.3
1000 ±60.0 ±6.0 30.0 3.0
Finnpipette F2 Fixed Volume Single Channel
Fixed Volume Inaccuracy Imprecision
µl µl % s.d.µl cv%
1 ±0.050 ±5.00 0.050 5.00
5 ±0.125 ±2.50 0.075 1.50
10 ±0.120 ±1.20 0.080 0.80
20 ±0.20 ±1.00 0.10 0.50
25 ±0.50 ±2.00 0.20 0.80
50 ±0.50 ±1.00 0.20 0.40
100 ±0.80 ±0.80 0.30 0.30
200 ±1.60 ±0.80 0.60 0.30
250 ±4.00 ±1.60 1.50 0.60
500 ±4.00 ±0.80 1.50 0.30
1000 ±8.00 ±0.80 3.00 0.30
2000 ±16.0 ±0.80 6.00 0.30
3000 ±40.0 ±1.33 15.00 0.50
5000 ±40.0 ±0.80 15.00 0.30
10000 ±60.0 ±0.60 30.00 0.30
Finnpipette F2 Variable Volume Multichannel
Range Channel Volume Inaccuracy Imprecision
µl µl % s.d.µl cv%
1-10 µl 8, 12, 16 10 ±0.24 ±2.4 0.16 1.6
1 ±0.24 ±24 0.16 16
5-50 µl 8, 12, 16 50 ±1.0 ±2.0 0.4 0.8
5 ±1.0 ±20 0.4 8.0
10-100 µl 8, 12 100 ±0.80 ±0.8 0.30 0.3
10 ±0.80 ±8.0 0.30 3.0
30-300 µl 8, 12 300 ±8.0 ±2.7 3.0 1.0
30 ±8.0 ±26.7 3.0 10.0

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Manufacturer specification limits
Finnpipette F2 Variable Volume Single Channel
Range Volume Inaccuracy Imprecision Factory
µl µl % s.d. µl cv% calibration tip
0.2-2 µl 2 ±0,050 ±2,50 0,040 2,00 Flex 10
0,2 ±0,024 ±12,00 0,020 10,00
0.5-5 µl 5 ±0,075 ±1,50 0,050 1,00 Flex 10
0,5 ±0,030 ±6,00 0,025 5,00
1-10 µl, micro 10 ±0,100 ±1,00 0,050 0,50 Flex 10
1 ±0,025 ±2,50 0,020 2,00
1-10 µl 10 ±0,100 ±1,00 0,080 0,80 Flex 200
1 ±0,035 ±3,50 0,030 3,00
2-20 µl, micro 20 ±0,20 ±1,00 0,08 0,40 50
2 ±0,06 ±3,00 0,05 2,50
2-20 µl 20 ±0,20 ±1,00 0,08 0,40 Flex 200
2 ±0,06 ±3,00 0,05 2,50
5-50 µl, micro 50 ±0,30 ±0,60 0,15 0,30 50
5 ±0,15 ±3,00 0,125 2,50
5-50 µl 50 ±0,30 ±0,60 0,15 0,30 Flex 200
5 ±0,15 ±3,00 0,125 2,50
10-100 µl 100 ±0,80 ±0,80 0,20 0,20 Flex 200
10 ±0,30 ±3,00 0,10 1,00
20-200 µl 200 ±1,2 ±0,60 0,4 0,20 Flex 200
20 ±0,36 ±1,80 0,14 0,70
100-1000 µl 1000 ±6,0 ±0,60 2,0 0,20 Flex 1000
100 ±1,0 ±1,00 0,6 0,60
0,5-5 ml 5000 ±25,0 ±0,50 10,0 0,20 5 ml
500 ±10,0 ±2,00 4,0 0,80
1-10 ml 10000 ±50,0 ±0,50 20,0 0,20 10 ml
1000 ±20,0 ±2,00 8,0 0,80
Finnpipette F2 Fixed Volume Single Channel
Fixed Volume Inaccuracy Imprecision Factory
µl µl % s.d. µl cv% calibration tip
1 ±0,040 ±4,00 0,040 4,00 Flex 10
5 ±0,070 ±1,40 0,070 1,40 Flex 10
10 ±0,090 ±0,90 0,080 0,80 Flex 200
20 ±0,14 ±0,70 0,10 0,50 Flex 200
25 ±0,15 ±0,60 0,125 0,50 Flex 200
50 ±0,30 ±0,60 0,20 0,40 Flex 200
100 ±0,40 ±0,40 0,30 0,30 Flex 200
200 ±0,80 ±0,40 0,60 0,30 Flex 200
250 ±1,0 ±0,40 0,75 0,30 Flex 1000
500 ±1,5 ±0,30 1,5 0,30 Flex 1000
1000 ±3,0 ±0,30 3,0 0,30 Flex 1000
2000 ±6,0 ±0,30 4,0 0,20 5 ml
3000 ±9,0 ±0,30 6,0 0,20 5 ml
4000 ±12,0 ±0,30 8,0 0,20 5 ml
5000 ±15,0 ±0,30 10,0 0,20 5 ml
10000 ±30,0 ±0,30 20,0 0,20 10 ml

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+
Finnpipette F2 Variable Volume Multichannel
Range Channels Volume Inaccuracy Imprecision Factory
µl µl % s.d. µl cv% calibration tip
1.0-10 µl 8, 12, 16 10 ±0,240 ±2,40 0,160 1,60 Flex 10
1 ±0,120 ±12,00 0,080 8,00
5-50 µl 8, 12, 16 50 ±0,75 ±1,50 0,35 0,70 Flex 200
5 ±0,25 ±5,00 0,10 2,00
10-100 µl 8, 12 100 ±1,30 ±1,30 0,50 0,50 Flex 200
10 ±0,50 ±5,00 0,20 2,00
30-300 µl 8, 12 300 ±3,0 ±1,00 0,9 0,30 Flex 300
30 ±1,5 ±5,00 0,6 2,00
Adjustment
Adjustment is done only for one volume. The recommended adjustment volume is the minimum
volume or 10% of the maximum volume. Multichannel pipettes adjustment check prior
calibration is done using one of the middle channels.
1. Place the service tool into the openings of the
calibration nut at the top of the handle.
2. Turn the service tool clockwise to increase, or
counterclockwise to decrease the volume.
3. After adjustment check the calibration according
to the instructions above.
Formulas for calculating results
Conversion of mass to volume
V = (w + e) x Z V = volume (µl)
w = weight (mg)
e = evaporation loss (mg)
Z = conversion factor for µl/mg conversion
Evaporation loss can be significant with low volumes. To determine mass loss, dispense water
to the weighing vessel, note the reading and start a stopwatch. See how much the reading
decreases during 30 seconds (e.g. 6 mg = 0.2 mg/s).
Compare this to the pipetting time from taring to reading. Typically pipetting time might be 10
seconds and the mass loss is 2 mg (10 s x 0.2 mg/s) in this example. If an evaporation trap or lid
on the vessel is used the correction of evaporation is usually unnecessary.
The factor Z is for converting the weight of the water to volume at test temperature and pressure.
A typical value is 1.0032 µl/mg at 22°C and 95 kPa. See the conversion table on page 26.
Inaccuracy (systematic error)
Inaccuracy is the difference between the dispensed volume and the selected volume of a pipette.
A = V - V0A = inaccuracy
V = mean volume
V0= nominal volume
Inaccuracy can be expressed as a relative value: A% = 100% x A / V0
Imprecision (random error)
Imprecision refers to the repeatability of the pipettings. It is expressed as standard deviation (s)
or coefficient of variation (cv)
s = standards deviation
v = mean volume
n = number of measurements
Standard deviation can be expressed as a relative value (CV)
CV = 100% x S / V

12
Maintenance
When the Finnpipette F2 is not in use, make sure it is stored in an upright position. We
recommend a Finnpipette stand for this purpose.
The part # refer to exploded views beginning at page 27.
Short-term service
The pipette should be checked at the beginning of each day for dust and dirt on the outside
surfaces of the pipette.
Particular attention should be paid to the tip cone. No other solvents except 70 % ethanol should
be used to clean the pipette.
Long-term service, single channel pipettes
Annual pipette usage varies significantly among laboratories, and the
types of substances being aspirated and dispensed also vary tremendously
among laboratories. In order to maintain proper care of the pipette, it is the
laboratory’s responsibility to develop a proper maintenance program that
meets its specific needs and requirements.
The servicing procedure starts with the disassembly of the pipette.
1-1000 µl pipettes
1. Press the tip ejector.
2. Rotate the tip ejector 11 counterclockwise and pull it out.
3. Turn out the tip cone counterclockwise with the service tool.
4. Pull out the piston and other parts. Push out with piston the rest of the
piston assembly. Then turn the tip cone upside down and tap all parts
from tip cone. Remember keep all parts in order on table for reassembly.
5. Clean the piston, the piston spring and the O-rings with a dry
napless cloth.
6. Check the tip cone for foreign particles.
7. Grease the cleaned parts with the lubricant that comes with
the pipette.
8. Reassemble the pipette components.
0.2-2µl, 0.5-5µl & 1-10 µl: First, slide spring 22,
o-ring support 23 and o-ring 24 on the tube 21.
With the 0.2-2µl model insert the tube 27 into
the tube 21. Then slide the spring 13, spring
support 16 and tubes 17 and 18, bigger o-ring 19
and smaller o-ring 20 back on the piston.
Compress the spring with fingers by pressing
piston and spring support 16 against each other
and slide the tube 21 with rest of the parts on
the
piston. Hold the spring compressed and
carefully slide the entire assembly into the tip
cone and release the spring.
2-20 µl & 5-50 µl: Slide the spring 13, spring
support 16 and tubes 17 and 18, bigger o-ring
19 and smaller o-ring 20 back on the piston.
Compress the spring with fingers by pressing
piston and spring support 16 against each
other and slide the bigger o-ring 19, smaller
o-ring 20, spring support 21 and the spring
22
(smaller diameter against spring support 21) on the piston. Hold the spring compressed and
carefully slide the entire assembly into the tip cone and release the spring.
10-100 µl & 20-200 µl: Slide the spring 13, spring support 16 and o-ring 17 back on the
piston. Slide the entire assembly into the tip cone.
100-1000µl: Put the o-ring 17 and support ring 16 to the tip cone. Slide the spring 13 on
the piston and slide the entire assembly into thetip cone.
1
3
2
1-200µl 200-1000µl

13
9. All: Put the spring 15 and support 14 on top of the tip cone and carefully insert the tip
cone assembly to the handle. Attach the tip cone by turning it first clockwise tight by hand,
then do the final tightening with the service tool.
10. Reassemble the tip ejector.
0.5-5ml & 1-10 ml pipettes
1. Press the tip ejector.
2. Rotate the tip ejector 10
counterclockwise to open it.
3. Disassemble the lower part of the tip
ejector 14 (snap fitting).
4. Turn the cylinder 13 counterclockwise
and pull out the tip cone assembly.
5. Remove the cylinder 13 by pressing
the snaps fittings of the cylinder.
5. Clean and regrease the O-ring 12 and cylinder 13.
7. Assemble the parts in the opposite order of disassembly.
Long-term service, multichannel pipettes
If the pipette is used daily it should be checked and lubricated
every three months.
1. Place the service tool head#1 between ring 15 and ejector 23. Push the tool
until the partssnap from each other.
2. Check that the ejector lever is in up position and pull down the tip ejector part
of the module. Place the service tool head#2 in the hole of adapter tube 46.
3. Open the upper end of the tip ejector slightly and remove the tip ejector.
4. Screw out the module of the handle.
5. Pull out the module spring 19 and clip 22.
6. Press the spring 13 and remove the locking pieces 12 from the
groove. Remove the spring 13.
7. Take off the locking claws 44 and 45 and pull out the adapter
tube and tube 43.
8. Use a screwdriver to remove the four screws in the module
cover and lift off the cover.
9. Remove the piston bar and clean the pistons and tip cones with a dry nap–free cloth.
10. If needed, service the tip cones:
16-channel 1-10µl: The tip cones cannot be serviced, please replace if necessary.
30-300 µl, 10-100µl & 5-50 µl: Open the tip cone by carefully releasing the cover ring from
its snap joint with the screwdriver. Remove all the parts from the tip cone. Clean all the
parts. If needed, replace the o-rings. Take one piston. Slide the spring 33, cover ring 32
(larger hole), spring 34, support ring 35, (o–ring 37 bigger 5-50µl/10-100µl) and o–ring 36
(smaller) onto the piston. Grease the o–ring with the lubricant provided in the pipette
package. Slide all the parts into the tip cone and close the snap joint of the cover ring.
1–10 µl: Open the tip cone by carefully releasing the cover ring from its snap joint with
the screwdriver. Remove all the parts from the tip cone. Clean all the parts. If needed,
replace the o-rings. Take one piston. Slide spring 33, cover ring 32 (larger hole), support 35,
o–ring 36 (bigger), o–ring 37 (smaller) and o–ring support 38 onto the piston. Then slide
spring 39, spring support 40 (sharp edges first) and o–ring 41 onto the o–ring sup port 38.
Grease the o–rings with the lubricant provided in the pipette package. Slide all the parts
into the tip cone and close the snap joint of the cover ring.
11. Install the piston bar with pistons and tip cones in the cover. Place aligning studs to the same
side when assembling the module. Close the cover with the four screws. Insert the clip 22.
12. Place the adapter tube and tube 43 on the neck of the module and insert the locking claws
44 and 45. Insert the module spring 19.
13. Insert spring 13 and locking pieces 12 to the piston rod 16.
14. Place the tip ejector on the module. Push the spring 19 inside tip ejector parts and close
the upper end of the ejector and keep closed with fingers.
15. Screw the module in the handle and tighten with service tool head#2.
16. Push the tip ejector lever down, until you hear a “click”.
1
2
3

14
Defect
Leakage
Inaccurate
dispensing
Piston jammed
Inaccurate
dispensing with
certain liquids
Possible reason
Tip incorrectly attached
Foreign particlesbetween tip and
tip cone
Foreign particles between the
piston, the O-ring and the cylinder
Insufficient amount of grease on
cylinder and O-ring
O-ring damaged
Incorrect operation
Tip incorrectly attached
Calibration altered: caused by
misuse, for example
Tip cone (Single channel) or
module (Multichannel) loose
Pipette has been unused for a long period
Highly volatile solvent is pipetted
repeatedly cleaning the grease
Unsuitable calibration
High viscosity liquids may require
recalibration
Solution
Attach firmly
Clean tip cones attach new tips
Clean and grease O-ring and
cylinder.
Grease accordingly
Change the O-ring
Follow instructions carefully
Attach firmly
Recalibrate according to
instructions
Tighten the tip cone or module
with the service tool
Remove tip if attached. Press
the plunger to the second stop
and release several times to re-
spread the grease
Recalibrate with the liquids
in question
CAUTION!
The Finnpipette is designed to allow easy in-lab service. If you would prefer to
have us or your local representative service your pipette, please make sure that
the pipette has been decontaminated before you send it to us.
Please note that the postal authorities in your country may prohibit or restrict the
shipment of contaminated material by mail.
Service Instructions for Multichannel Pipette Tip Cones
To ensure even performance between all channels in a multichannel pipette, all tip cones have
to be changed at the same time, if any of them needs to be changed. Don’t mix tip cones of
different packages, because one bag contains a matched set of tip cones. Place aligning studs
to the same side when assembling the module. See picture on page 35.
Sterilization
The entire pipette can be sterilized by autoclaving it at 121°C (252°F) (2 ata) (minimum 20
minutes). No special preparations are needed for autoclaving. You can use steam sterilization
bags if needed.
After autoclaving the pipette must be cooled to room temperature for at least two hours. Before
pipetting, make sure that the pipette is dry. We recommend that you check the calibration after
every sterilization cycle to achieve the best possible accuracy.
Trouble shooting
The table below lists possible problems and their solutions.

15
产品简介
Finnpipette F2是一种一般用途的微量移液器，可通过连续调节准确吸取和转移
液体。
它利用空气置换原理（即使用空气作为分隔），并使用可拆卸、一次性使用的吸嘴。
手柄上的读数窗口可对经过调节的移液量进行数字显示。
Finnpipette F2可调量程移液器的十三种不同型号涵盖了0.2 µl至10 ml之间的所
有量程范围。
订购号码 量程范围 色标 Finntip
4642010 0,2 µl to 2 µl pink Flex 10, 10, 20, 50
4642020 0,5 µl to 5 µl pink Flex 10, 10, 20, 50
4642030 1 µl to 10 µl pink Flex 10, 10, 20, 50
4642040 1 µl to 10 µl yellow
Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4642050 2 µl to 20 µl turquoise 50
4642060 2 µl to 20 µl yellow Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4642120 5 µl to 50 µl turquoise 50
4642130 5 µl to 50 µl yellow Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4642070 10 µl to 100 µl yellow Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4642080 20 µl to 200 µl yellow Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4642090 100 µl to 1000 µl blue Flex 1000, 1000, 1000 Ext
4642100 0,5 ml to 5 ml green 5 ml
4642110 1 ml to 10 ml red 10 ml, Flex 10 ml Ext
Finnpipette F2固定量程移液器程的十五种不同型号涵盖了1 µl至10 ml之间的所
有量程范围。
订购号码 量程范围 色标 Finntip
4652000 1 µl dark blue Flex 10, 10, 20, 50
4652010 5 µl dark blue Flex 10, 10, 20, 50
4652020 10 µl dark blue Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4652130 20 µl dark blue Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4652030 25 µl dark blue Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4652040 50 µl dark blue Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4652050 100 µl dark blue Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4652140 200 µl dark blue Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4652060 250 µl dark blue Flex 1000, 1000, 1000 Ext
4652070 500 µl dark blue Flex 1000, 1000, 1000 Ext
4652080 1000 µl dark blue Flex 1000, 1000, 1000 Ext
4652090 2000 µl dark blue 5 ml
4652100 3000 µl dark blue 5 ml
4652110 5000 µl dark blue 5 ml
4652120 10000 µl dark blue 10 ml, Flex 10 ml Ext
Finnpipette F2多通道移液器的十种不同型号涵盖了 1 µl 至 300 μl 之间的所
有量程范围。
订购号码 道数 量程范围 色标 Finntip
4662000 8 1 µl to 10 µl pink Flex 10, 10, 20, 50
4662010 8 5 µl to 50 µl yellow Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4662020 8 10 µl to 100 µl yellow Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4662030 8 30 µl to 300 µl orange Flex 300, 300
4662040 12 1 µl to 10 µl pink Flex 10, 10, 20, 50
4662050 12 5 µl to 50 µl yellow Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4662060 12 10 µl to 100 µl yellow Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4662070 12 30 µl to 300 µl orange Flex 300, 300
4662080 16 1 µl to 10 µl purple 20
4662090 16 5 µl to 50 µl turquoise 50

16
数码显示
手柄上的大号字体显示屏可清晰显示经过调节后
的移液量。
原材料
Finnpipette F2由耐用、抗化学腐蚀的原材料制造而成。
吸嘴连件可在 121°C 下重复进行高温消毒。
吸嘴简介
建议将Finntips与Finnpipette F2配合一起使用。
由全新的纯聚丙烯制成，聚丙烯通常被认为是适于制造吸嘴的唯一一种无污染
材料。 Finntips可耐高温高压消毒（121°C）。
包装
Finnpipette F2的包装经过特别设计，套装内包括以下物品：
1. Finnpipette移液器
2. 维修工具
3. 多道维修工具
4. 油脂 1g (订货号：3300200)
5. 说明书
6. 校准证书
安全
此符号代表注意 .
用途
此设备的用途是用来移取范围在0,2 μl 到 10 ml之间的液体
Finnpipette F2 以及 Finntips的设计是作为分析系统的一部分， 为那些负责
校验系统的终端客户可以得到可靠及安全的结果.
对健康的危害
对于危害的预防以及安全说明请遵循一般流程，例如：穿防护服，配带防
护眼镜及手套.
对于有害物质的使用以及废弃物的处理（例如：带有辐射的或者有潜在传
染性的物质），请遵循相关的安全说明或者一般实验室规范。.
移液器以及吸嘴不能在体内使用。 请不要使用移液器吸取液体后再注射入
人体内。
请不要在弹出吸嘴时，将吸嘴对着任何人。
Finnpipette F2 的使用者需要具备实验室操作技术并通过培训的人员。在使用
移液器及吸嘴之前或者使用过程中必须先阅读使用说明。 F2 移液器可以在
+4°C 到 +40°C之间进行使用
错误的移液结果
由于以下因素，可能会对性能造成偏差：
a. 移液方式（推荐使用正向的移液方法）
b. 温度（空气，液体，容器，移液器以及吸嘴）
c. 气压
d. 湿度
e. 操作人员， 例如：拇指的运动，移液时的角度
f. 液体的稠密度，粘度以及蒸气压力
g. 吸嘴的型号

17
+
如果移液器的性能对于某个特定应用的输出至关重要，那么必须用其他可
替代的测试对结果进行确认。
如果没有这样的替代选择，那么需要通过重复测试来进行确认。移液过程
中不正确移液量的可能性还是无法完全避免的。
为了避免错误的注入或者漏液，请检查吸嘴是否完全安装到移液器上。
当旋转移液量调整按钮时，请不要超出移液器的液量范围。 否则会导致移
液器的损坏或者影响移液性能。
如果吸嘴再次被使用，那么移液器的性能将无法保证。
使用时，请选择颜色标记相匹配的吸嘴与移液器。
材料的处理
请遵循实验室或者国家特定的流程来处理具有生物危害或者带有辐射的废弃
物。处理具有传染性的物质，请参考当地法规。
警告 吸嘴可能具有潜在的传染性。请将所有使用过的一次性吸嘴按照生物危
害废弃物的要求处理。
警告 在处理移液器前请先进行消毒。
如果想要将之废弃，请与当地环保部门联系。如果想要了解更多信息，请联系
我们info.pipettes@thermofisher.com, 或者联系当地Thermo Fisher Scientific（赛默
飞世尔科技）代表。
关于原包装和包装材料，请使用您所知道的回收经营者。
移液器的操作
设定移液量
1. 使用移液器顶部的按钮设定移液量。如需增加移液
量，应按逆时针方向旋转按钮。如需减少移液量，
应按顺时针方向旋转按钮。
2. 应确保移液量设定到位且完整读取显示窗口上的读数。
3. 勿将液量设定在移液器指定的容量允许范围以外。
使用蛮力过度旋转按钮可能会导致卡壳并最终使移
液器受损。
安全标签
可在安全标签上记下移液器的用途、使用者姓名
缩写、校准日期等。可用较细的针将标签取下。
用笔在标签上书写，然后将标签放回原处。
吸嘴的卸除
为防止污染，移液带有管嘴推出器。如需卸下吸
嘴，应将移液器指向适当的废液槽，然后用拇指
按下管嘴推出器。
移液方法
始终应缓慢按动和释放按钮，尤其是在处理高粘度液体时。切勿任由
按钮自动反弹。
应确保将吸嘴牢固安装在管嘴连件上。检查吸嘴内是否有杂质。
在进行实际的移液操作前，应先重复2-3次将该溶液吸入并排出吸嘴。
吸液时应使移液器保持竖直。应用食指抵住放松指靠。
务必使吸嘴、移液器和溶液保持同一温度。

18
正向移液方法
用于将液体注入干净的试剂容器内。
1 按下按钮至第一停点处。
2. 将吸嘴探入容器内液体表面下约1 cm处，
然后慢慢释放按钮。将吸嘴从液体中取
出并靠在容器边缘以去除多余的液体。
3. 轻轻按下按钮至第一停点即可获得所需
的液体容量。大约等候1秒钟以后，
即可继续将按钮按到底。这样做可排空
吸嘴内的液体。
4. 释放按钮使其还原至备用状态。如有必要，
可更换吸嘴然后继续进行移液操作 。
反向移液方法
反向移液方法比较适合用于粘度较大或较容易产生泡沫的液体。也建议在微量
的移液操作中使用此技巧。将液体注入干净的试剂容器内。
1. 按下按钮到底。
2. 将吸嘴探入容器内液体表面下约1 cm处，
然后慢慢释放按钮。这样做可以将液
体吸入吸嘴。将吸嘴从液体中取出并靠
在容器边缘以去除多余的液体。
3. 轻轻按下按钮至第一停点即可获得事先设
定的液量。在第一停点处按住按钮。
吸嘴内会有部分残留液体，不应将其计入
实际的移液量。
4. 应将残留液体与吸嘴一起丢弃或将其重新
注入原容器内。
反复移液方法
反复移液方法是用于重复提取相同容量液体的一种快速简单的方法。将用于移
液的液体注入干净的试剂容器内。
1. 按下按钮到底。
2.
将吸嘴探入容器内液体表面下约1 cm
处， 然后慢慢释放按钮。这样做可以将
液体吸入吸嘴。将吸嘴从液体中取出并
靠在容器边缘以去除多余的液体。
3. 轻轻按下按钮至第一停点即可获得事
先设 定的液体容量。在第一停点处按住按
钮。 吸嘴内会有部分残留液体，不应将
其计入实际的移液量。
4. 可通过重复第3和第4步操作继续进行移液。
非均质液样的移液操作
（全血移液法）
使用正向移液方法的第1和第2步骤将血液吸入吸嘴。
使用清洁干燥的薄纸仔细将吸嘴擦干。
1. 将吸嘴伸入试剂内并按动按钮至第一停
点，同时应保证吸嘴始终处于液面以下。
2. 慢慢释放按钮使其还原至备用状态。
这样做可将液体吸入吸嘴。使吸嘴始终
处于溶液中。
3. 按动按钮至第一停点，然后慢慢释放按钮。
重复此步骤直至吸嘴的内壁变得清晰。
4. 最后，将按钮按到底以将吸嘴内的液体
完全排出。
1 2 3 4 5
1 2 3 4
1 2 3 4
1 2 3 4

19
校准与调节
所有的Finnpipette产品均已在工厂经过校对和调节，以便通过正向移液方法提
供规定容量的蒸馏水或去离子水。使用其它移液方法可能会影响校准结果。因此
移液器在使用其它移液方法或用于不同温度和粘度的液体时，可进行重新调节。
仪器与试验要求
必须使用分析天平。应根据移液器内的试验液量选定天平的刻度精确度：
液量范围 刻度精确度
少于10 µl 0.001 mg
10-100 µl 0.01 mg
多于100 µl 0.1 mg
试验液：经蒸馏或去离子、ISO 3696标准3级水。试验应在无空气流动
的室内进行， 并应使水、移液器和空气温度恒定在15°C与30°C之间，误差在
±0.5℃上下。相对湿度必须大于50%。特别是液量在50µl以下时，空气湿度
越高越好，以便减少蒸发作用对液量产生的影响。 建议使用专门的辅助仪器，
如加湿器。
校准检查的步骤
应检查移液器的最大液量（标定液量）和最小液量。新的吸嘴应预湿3-5次，
并对最大和最小两种液量各进行十次移液操作。每次移液（Ex）时都应对移液
器进行调节以获得所需的液量。建议使用正向移液方法。允许的最大误差值专
为正向操作方法而设计。建议对多道移液器的每一个通道都进行校准，但是仅
去校准两边最外侧的通道是比较合适的校准方法。在校准之前，请先通过使用
中间的某一个通道来进行调整检查。建议使用下表中ISO8655的校验标准或者
用户自定义标准。
步骤：
1. 进行一系列10次最小液量的移液操作。
2. 进行一系列10次最大液量的移液操作。
3. 计算以上两个系列操作的错误率（A）和不精确率（cv）。
4. 将结果与表1内的限值进行对照。
如果计算值在对应的限值范围内，则表示对移液器进行了正确的调节。

20
ISO8655 校验规格
Thermo Scientific Finnpipette F2 的规格符合ISO8655的标准。
Finnpipette F2 可调式量程单道
量程 液量 错误率 不精确率
µl µl % s.d. µl cv%
0,2-2 µl 2 ±0.080 ±4 0.040 2.0
0.2 ±0.080 ±40 0.040 20.0
0,5-5 µl 5 ±0.125 ±2.5 0.075 1.5
0.5 ±0.125 ±25 0.075 15
1-10 µl 10 ±0.120 ±1.2 0.080 0.8
1 ±0.120 ±12 0.080 8.0
2-20 µl 20 ±0.20 ±1.0 0.10 0.5
2 ±0.20 ±10.0 0.10 5.0
5-50 µl 50 ±0.50 ±1.0 0.20 0.4
5 ±0.50 ±10 0.20 4.0
10-100 µl 100 ±0.80 ±0.8 0.30 0.3
10 ±0.80 ±8.0 0.30 3.0
20-200 µl 200 ±1.60 ±0.8 0.60 0.3
20 ±1.60 ±8.0 0.60 3.0
100-1000 µl 1000 ±8.0 ±0.8 3.0 0.3
100 ±8.0 ±8.0 3.0 3.0
0,5-5 ml 5000 ±40.0 ±0.8 15.0 0.3
500 ±40.0 ±8.0 15.0 3.0
1-10 ml 10000 ±60.0 ±0.6 30.0 0.3
1000 ±60.0 ±6.0 30.0 3.0
Finnpipette F2 固定式量程单道
固定液量 错误率 不精确率
µl µl % s.d.µl cv%
1 ±0.050 ±5.00 0.050 5.00
5 ±0.125 ±2.50 0.075 1.50
10 ±0.120 ±1.20 0.080 0.80
20 ±0.20 ±1.00 0.10 0.50
25 ±0.50 ±2.00 0.20 0.80
50 ±0.50 ±1.00 0.20 0.40
100 ±0.80 ±0.80 0.30 0.30
200 ±1.60 ±0.80 0.60 0.30
250 ±4.00 ±1.60 1.50 0.60
500 ±4.00 ±0.80 1.50 0.30
1000 ±8.00 ±0.80 3.00 0.30
2000 ±16.0 ±0.80 6.00 0.30
3000 ±40.0 ±1.33 15.00 0.50
5000 ±40.0 ±0.80 15.00 0.30
10000 ±60.0 ±0.60 30.00 0.30
Finnpipette F2 可调式量程多道
量程 道数 液量 错误率 不精确率
µl µl % s.d.µl cv%
1-10 µl 8, 12, 16 10 ±0.24 ±2.4 0.16 1.6
1 ±0.24 ±24 0.16 16
5-50 µl 8, 12, 16 50 ±1.0 ±2.0 0.4 0.8
5 ±1.0 ±20 0.4 8.0
10-100 µl 8, 12 100 ±0.80 ±0.8 0.30 0.3
10 ±0.80 ±8.0 0.30 3.0
30-300 µl 8, 12 300 ±8.0 ±2.7 3.0 1.0
30 ±8.0 ±26.7 3.0 10.0