Thermo Scientific Sorvall Legend Micro 21R User manual

OperatingtheThermoScientificSorvallLegendMicro21RCentrifuge
Switchingonthecentrifuge
Setthemainpowerswitchoftheinstrument.Thedisplayshowsthattheinstrumentisgoingthroughan
internalcheckofitssoftware.Afterthischeckthedisplayswitchedintothecurrentvaluemode.The
speedandtheremainingruntimeshow0;therefrigerationunitdisplayshowsthecurrenttemperature
ofthesample.

Openingthelid
Themainpowermustbeontoopenthelid.Toopenthelidpressthe“open‐lid”button.

Closingthelid
Thecentrifugelidislockedbyslightlypressingdownthefrontpartofthelid.DONOTSLAMTHELID!
Fillingthecentrifugetubes
Thesmallerthecentrifugeimbalance,thebettertheseparation,becauseseparatezonesarenolonger
perturbedbyvibration.Itisthereforeimportanttobalancethecentrifugetubesaswellaspossible.To
minimizeimbalanceyoushouldfillthetubesasevenlyaspossible.Youcanachievethisbyeye,
however,youmustnonethelessensurethatoppositetubesarefilledtothesamelevel.
Placingthetubesintherotor
Therotormustbeloadedsymmetrically.Whenloadingtherotoronlypartially,youmustensurethat
oppositeboresalwaysreceivetubesofequalweight(whencentrifugingasinglesample,placea
centrifugetubee.g.filledwithwateropposite).Thefollowinggivesexamplesforproperloading:


Thefollowingareexamplesofimproperlyloadedrotors:

Afteryouhaveloadedthetubesclosetherotorlid.Afteryouhaveclosedtherotorlidclosethe
centrifugelid.
EnteringParameters
SwitchingfromspeedtoRCF(relativecentrifugalforce)display
Uponturningonthecentrifugethespeeddisplayisset.Byactivatingtheselectionkeyyoucanswitchto
theRCF‐valueorchangebetweenrpmandRCFdisplays:

Selectingspeed
Thecentrifugespeedcanbesettoaminimumof300rpm.Youcanadjustthespeedin100rpm
increments.Proceedasfollows:
1. Pressoneofthesetkeys(˄forincreaseor˅fordecrease)inthefieldofspeedofthecontrol
panel.
2. Ifyouholdthekeypressed,thedisplaychangescontinuouslyatfirstslowly,andafterafew
secondsatanacceleratedpacetothehigherorlowervalues.
3. Releasethekeyassoonasyouhavereachedthedesiredvalueandfinetuneifnecessaryby
repeatedlybrieflypressingonofthekeys.Thefirstdigitbehindthedecimalpointflashesfora
fewsecondsandthenswitchespermanentlyintothecurrentvaluemode.Thenewpre‐selected
speedisnowstored.
EnteringtheRCFvalue
YoucanadjusttheRCFpre‐selectedvalueinstepsof100g.TheRCFvaluecanbesettoaminimumof
100xg.YoucanadjusttheRCFvaluesthesameasyouadjusttherpmvalues.ThedisplayedRCFvalueis
alwayscorrespondingtothemaximumofcentrifugeradiusoftheMicroliterrotor24x2mL.Forother
rotorspleasecalculatetheRCFvalue.
Selectingtheruntime
Youcanselectaruntimebetween1and99minutesorcontinuousoperation[hd].
Runtimeselection

Proceedasfollows:
1. Pressoneofthe“set”keys(˄forincreaseor˅fordecrease)inthefieldofmeofthecontrol
panel.
2. Ifyoukeepthekeypressed,thedisplaychangesatfirstslowlyandafterafewsecondsatan
acceleratedpacetothehigherorlowervalue.
3. Releasethekeyassoonasyouhavereachedthedesiredvalueandfinetuneifnecessaryby
repeatedlybrieflypressingonofthekeys.Theruntimedisplayflashesforanumberofseconds,
thenchangestopermanentdisplayofthecurrentvaluemode.Thenewpre‐selectedruntimeis
nowstored.
Continuousoperation
Tooperatethecentrifugeinthecontinuousmode,youmustpressandkey(˅)unlthedisplaychanged
to“hd”for“hold”.Inthismodethecentrifugerunsuntilyoustoptherunbymanuallypressingthe
“stop”button.
Settingthetemperature
Todeterminethesampletemperature,operateasadvised:
1. Pressoneofthe“set”keys(˄forincreaseor˅fordecrease)inthefieldof“temperature”ofthe
controlpanel.Bypressingthekeybriefly,youincreaseordecreasethetemperatureinstepsof1
˚C.Thisoptionissupposedtobeusedforsmallchangesandfinetuning.
2. Ifyoukeepthekeypressed,thedisplaychangesatfirstslowlyandafterafewsecondsatan
acceleratedpacetothehigherorlowervalues.
3. Releasethekeyassoonasyouareclosetothedesiredvalueandfinetuneifnecessaryby
repeatedlybrieflypressingthekey.Thedisplayflashesforafewsecondsandthenturnsto
permanentdisplayofthecurrentvaluemode.Thenewpre‐selectedtemperatureisnowstored.
Therefrigerationstartsoperatingafterclosingthecentrifugelid,atonceifthepre‐selectedtemperature
isbelowthecurrentlytemperatureoftherotorchamber.