Thermo Scientific BioCane CK50900 Series User manual

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User Manual
BioCane™ Type CK50900
Cryobiological Storage essel
peration Manual and Parts List LT509X17 (7000807) Rev. 9

Thermo Scientific
Manual Number LT509X17 (7000807)
9 41864 11/07/17 Removed CE mark, number bpg
8 41238 12/20/16 Combined languages to one file slb
7 40578 2/23/16 Clarified Do Not Roll warning ccs
6 -- 10/27/15 Updated symbols and labels, added cautions ccs
5 30559 11/12/13 Added warnings and cautions to pg 1-2, environmental conditions to pg 2-1 ccs
4 27797 9/21/11 Added manufacturer icon and euro rep ccs
3 27547 7/28/11 Updated specs - BioCane 73 vials from 2880 to 2016 ccs
2 27457/SI-10618 7/21/11 Added CE number to pg 2-1 ccs
1 -- 2/24/11 Added part numbers for replacement canisters ccs
Preface
Cryobiological Storage Vessel i
Catalog Number Model Number Name
CK509X2 807 BioCane 20
CK509X3 808 BioCane 34
CK509X4 809 BioCane 47
CK509X6 820 BioCane 73

Thermo Scientificii Cryobiological Storage Vessel
Preface
Contains Parts and Assemblies
Susceptible to Damage by
Electrostatic Discharge (ESD)
CAUTION
Important Read this instruction manual. Failure to read, understand and follow instructions in this
manual may result in damage to the unit, injury to operating personnel, and poor equipment
performance. s
Caution All internal adjustments and maintenance must be performed by qualified service personnel. s
Caution It is recommended to have this vessel tested by the manufacturer or qualified cryovessel service
technician every 7-10 years, regardless of any problems (or lack thereof) you may have had in the past.
This will help insure your samples against sudden loss of liquid nitrogen due to vacuum failure. s
Warning Nitrogen gas can cause suffocation without warning. Store and use liquid nitrogen only in a
well-ventilated place. As the liquid evaporates, the resulting gas displaces the normal air in the area.
(The cloudy vapor that appears when liquid nitrogen is exposed to the air is condensed moisture, not
the gas itself. The issuing gas is invisible.) In closed areas, excessive amounts of nitrogen gas reduces the
concentration of oxygen and can result in asphyxiation. Because nitrogen gas is colorless, odorless, and
tasteless, it cannot be detected by the human senses. Breathing an atmosphere that contains less than
19.5% oxygen can cause dizziness and quickly result in unconsciousness and death. Therefore, the use
of oxygen monitoring equipment is strongly recommended. s
Warning Contact of liquid nitrogen with the skin or eyes may cause serious (freezing) injury. s
Warning Do not roll. Always keep liquid nitrogen vessel in an upright position. Exercise caution to
prevent spilling and splashing. s
aterial in this manual is for information purposes only. The contents and the product it describes are subject
to change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this
manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to
the use of this manual.
In ended Use:
The Thermo Fisher Scientific cryogenic storage vessels are intended to store biological samples at cryogenic
temperatures. These products are comprised of a vessel and a lid, with or without a level monitor.
Unin ended Use:
These products are not intended for use within the patient environment.
When this manual is translated into other languages, the US English version is binding.
© 2010 Thermo Fisher Scientific. All rights reserved. Printed in U.S.A.

Thermo Scientific Cryobiological Storage Vessel iii
Preface
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Extreme temperature hazards. Only qualified persons should perform procedures associated with this
symbol.
Potential biological hazards. Proper protective equipment and procedures must be used.
WEEE Compliance: Thermo Fisher Scientific has contracted with companies for recycling/disposal in
each EU ember State. For further information, send an email to [email protected].
4Always use the proper protective equipment (clothing, gloves, goggles, etc.)
4Always dissipate extreme cold or heat and wear protective clothing.
4Always follow good hygiene practices.
4Each individual is responsible for his or her own safety.

Thermo Scientificiv Cryobiological Storage Vessel
Preface
Do You Need Information or Assistance on
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Our staff can supply technical information about proper setup, operation orService Support
troubleshooting of your equipment. We can fill your needs for spare or replacement parts or
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Warranty for your Thermo Scientific products.
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When more extensive service is necessary, we will assist you with direct factory trained
technicians or a qualified service organization for on-the-spot repair. If your service need is
covered by the warranty, we will arrange for the unit to be repaired at our expense and to your
satisfaction.
Regardless of your needs, our professional telephone technicians are available to assist you
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Thermo Fisher Scientific (Asheville) LLC
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International customers, please contact your local Thermo Scientific distributor.

Cryobiological Storage Vessel vThermo Scientific
Table of Contents
Safety Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Please Read . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
General Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
easuring Liquid Nitrogen Quantity . . . . . . . . . . . . . . . . . . . . . . . . 3-2
Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
Warranty Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
Section 1
Section 2
Section 3
Section 4
Section 5

Cryobiological Storage Vessel 1-1Thermo Scientific
Please Read
Section 1 Safety Information
This manual contains important operating and safety information. The
user must carefully read and understand the contents of this manual prior
to the use of this equipment.
Your Thermo Scientific cryogenic vessel has been designed with function,
reliability, and safety in mind. It is the user’s responsibility to install it in
conformance with local electrical codes. For safe operation, pay attention
to the warnings and cautions throughout the manual.
All cryopreservation vessels, regardless of who manufactures them, use a
vacuum to provide for the super insulative properties needed to keep
nitrogen in a liquid form. They are very similar, in fact, to the thermos
vessels you may have used to store coffee, soup or milk.
Remember how fragile they were? Since no vacuum is perfect or will last
forever, we suggest that you monitor the consumption of liquid nitrogen
used by your vessel on a regular basis, i.e. every 1-2 days. By monitoring,
you may be able to anticipate subtle changes in consumption and possibly
react to problems before they arise. You may opt to use a level monitor for
this purpose.
Also, if your samples are super-critical, one-of-a-kind, or irreplaceable,
consider allocating your samples into 2 separate vessels.
Remember the old adage about placing all your eggs into one basket…
Finally, because no vacuum will last forever, consider having your vessel
tested by a qualified cryovessel manufacturer every 7-10 years regardless of
any problems (or lack thereof) you may have had in the past. This will
help insure your samples against sudden loss of nitrogen due to vacuum
failure.

1-2 Cryobiological Storage Vessel Thermo Scientific
Section 1
Safety Information
Warnings
• This unit is intended to be used by trained personnel in a well-
ventilated environment.
• Liquid nitrogen is extremely cold; it boils at -196°C.
• Follow all necessary precautions and warnings relevant to applicable
hazards such as frostbite, contact burn, asphyxiation, etc. in cryogenic
areas.
• To avoid injury due to frostbite or ruptured vials, use extreme care
whenever handling liquid nitrogen, liquid nitrogen storage or transfer
vessels or any objects which have come in contact with liquid
nitrogen.
• Leave no areas of skin exposed.
• Wear adequate PPE where necessary: face shield, cryogenic gloves,
cryogenic apron.
• Only use the power supply provided with the unit.
• Do not tightly seal liquid nitrogen containers or prevent nitrogen gas
from escaping.
• Always handle vials with tongs.
• Use extreme care to prevent spilling and splashing liquid nitrogen
during transfer and removal of storage contents and holders.
• Immediately remove any clothing or safety attire on which liquid
nitrogen has been spilled or splashed .
• Get immediate medical attention for any frostbite injuries due to
liquid nitrogen.
• Never overfill liquid nitrogen vessels.
When inser ing or removing cans, be careful no o come in con ac
wi h he neck ube area of he vessel. Remove or inser cans slowly,
in a ver ical manner. Scra ches on he neck ube can cause prema ure
vacuum failure.
Do no amper wi h, or remove vacuum por (covered by black
plas ic cap on side of vessel); his will void warran y.
Do not spill liquid nitrogen on vacuum port - this can cause vacuum
failure.
Always keep liquid nitrogen in an upright position.

Cryobiological Storage Vessel 2-1Thermo Scientific
Section 2 General Specifications
* Actual working time may vary due to current atmospheric conditions, container
history, manufacturing tolerances, and any individual patterns of use.
Environmen al Condi ions
Operating temperature: 17°C - 27°C
20% to 80% relative humidity, non-condensing.
Installation Category II (overvoltage) in accordance with IEC 664.
Pollution Degree 2 in accordance with IEC 664.
Altitude limit: 2,000 meters.
Storage: -25°C to 65°C; 10% to 85% relative humidity.
Locator and Locator Plus units are intended for indoor use only.
BioCane 20 BioCane 34 BioCane 47 BioCane 73
Height (Inches) 25.70" 26.60" 26.50" 27.00"
Diameter (Inches) 14.50" 18.20" 20.00" 22.00"
LN2 Capacity 20.50L 34.80L 47.40L 73.0L
Static Holding Time (Days) 227 193 121 121
Static LN2 Evaporation Rate* 0.1L/day 0.18L/day 0.40L/day 0.60L/day
Liquid Phase Capacity 1.2 ml 180 vials 720 vials 1512 vials 2016 vials

2-2 Cryobiological Storage Vessel Thermo Scientific
Section 2
General Specifications
Unpacking Before using your new vessel, carefully inspect it. Check for signs of
damage which may have occurred in shipment. It is advisable to fill (see
Filling Instructions) all new units with liquid nitrogen and watch liquid
nitrogen loss rate for a few days. If there are any problems, call Technical
Services as soon as possible.
The on-line warranty registration address is listed on the front of this
manual. The unit should be registered within 30 days to make warranty
effective. This information must include the serial number which is located
on the handle of the vessel.
Note The most prevalent cause of failure of liquid nitrogen storage vessels
is mechanical. The vessel neck tube supports the full weight of the inner
shell and all liquid nitrogen it contains. A side or corner blow to the vessel
causes the inner shell to swing in a pendulum motion causing the neck
tube to be damaged. Any storage vessel which has been exposed to an
accident, has been dropped or lowered to hit on one corner will tend to fail
more rapidly than one which has not.
Caution Exercise caution when moving your BioCane™ vessel. BioCane
cryobiological storage systems are not transportation vessels. Transport
carts are designed for mobility within the lab or lab to lab only. oving
full vessels long distances, over cracks in floor, thresholds, on inclined
ramps or in elevators can cause premature vacuum failure.
Note If samples must be transported under cryogenic conditions, consider
the Thermo Scientific Arctic Express line of Dry Shippers.