Thermo Scientific BioCane CK50900 Series User manual

Visit us online to register your warranty
www.thermoscientific.com/labwarranty
User Manual
BioCane™ Type CK50900
Cryobiological Storage essel
peration Manual and Parts List LT509X17 (7000807) Rev. 9

Thermo Scientific
Manual Number LT509X17 (7000807)
9 41864 11/07/17 Removed CE mark, number bpg
8 41238 12/20/16 Combined languages to one file slb
7 40578 2/23/16 Clarified Do Not Roll warning ccs
6 -- 10/27/15 Updated symbols and labels, added cautions ccs
5 30559 11/12/13 Added warnings and cautions to pg 1-2, environmental conditions to pg 2-1 ccs
4 27797 9/21/11 Added manufacturer icon and euro rep ccs
3 27547 7/28/11 Updated specs - BioCane 73 vials from 2880 to 2016 ccs
2 27457/SI-10618 7/21/11 Added CE number to pg 2-1 ccs
1 -- 2/24/11 Added part numbers for replacement canisters ccs
Preface
Cryobiological Storage Vessel i
Catalog Number Model Number Name
CK509X2 807 BioCane 20
CK509X3 808 BioCane 34
CK509X4 809 BioCane 47
CK509X6 820 BioCane 73

Thermo Scientificii Cryobiological Storage Vessel
Preface
Contains Parts and Assemblies
Susceptible to Damage by
Electrostatic Discharge (ESD)
CAUTION
Important Read this instruction manual. Failure to read, understand and follow instructions in this
manual may result in damage to the unit, injury to operating personnel, and poor equipment
performance. s
Caution All internal adjustments and maintenance must be performed by qualified service personnel. s
Caution It is recommended to have this vessel tested by the manufacturer or qualified cryovessel service
technician every 7-10 years, regardless of any problems (or lack thereof) you may have had in the past.
This will help insure your samples against sudden loss of liquid nitrogen due to vacuum failure. s
Warning Nitrogen gas can cause suffocation without warning. Store and use liquid nitrogen only in a
well-ventilated place. As the liquid evaporates, the resulting gas displaces the normal air in the area.
(The cloudy vapor that appears when liquid nitrogen is exposed to the air is condensed moisture, not
the gas itself. The issuing gas is invisible.) In closed areas, excessive amounts of nitrogen gas reduces the
concentration of oxygen and can result in asphyxiation. Because nitrogen gas is colorless, odorless, and
tasteless, it cannot be detected by the human senses. Breathing an atmosphere that contains less than
19.5% oxygen can cause dizziness and quickly result in unconsciousness and death. Therefore, the use
of oxygen monitoring equipment is strongly recommended. s
Warning Contact of liquid nitrogen with the skin or eyes may cause serious (freezing) injury. s
Warning Do not roll. Always keep liquid nitrogen vessel in an upright position. Exercise caution to
prevent spilling and splashing. s
aterial in this manual is for information purposes only. The contents and the product it describes are subject
to change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this
manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to
the use of this manual.
In ended Use:
The Thermo Fisher Scientific cryogenic storage vessels are intended to store biological samples at cryogenic
temperatures. These products are comprised of a vessel and a lid, with or without a level monitor.
Unin ended Use:
These products are not intended for use within the patient environment.
When this manual is translated into other languages, the US English version is binding.
© 2010 Thermo Fisher Scientific. All rights reserved. Printed in U.S.A.

Thermo Scientific Cryobiological Storage Vessel iii
Preface
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Extreme temperature hazards. Only qualified persons should perform procedures associated with this
symbol.
Potential biological hazards. Proper protective equipment and procedures must be used.
WEEE Compliance: Thermo Fisher Scientific has contracted with companies for recycling/disposal in
each EU ember State. For further information, send an email to [email protected].
4Always use the proper protective equipment (clothing, gloves, goggles, etc.)
4Always dissipate extreme cold or heat and wear protective clothing.
4Always follow good hygiene practices.
4Each individual is responsible for his or her own safety.

Thermo Scientificiv Cryobiological Storage Vessel
Preface
Do You Need Information or Assistance on
Thermo Scientific Products?
If you do, please contact us 8:00 a.m. to 6:00 p.m. (Eastern Time) at:
1-740-373-4763 Direct
1-800-438-4851 Toll Free, U.S. and Canada
1-877-213-8051 FAX
http://www.thermofisher.com Internet Worldwide Web Home Page
[e-mail] Tech Support Email Address
Certified Service Web Pagewww.unitylabservices.com
Our staff can provide information on pricing and give you quotations. We canSales Support
take your order and provide delivery information on major equipment items or make
arrangements to have your local sales representative contact you. Our products are listed on the
Internet and we can be contacted through our Internet home page.
Our staff can supply technical information about proper setup, operation orService Support
troubleshooting of your equipment. We can fill your needs for spare or replacement parts or
provide you with on-site service. We can also provide you with a quotation on our Extended
Warranty for your Thermo Scientific products.
Whatever Thermo Scientific products you need or use, we will be happy to discuss your
applications. If you are experiencing technical problems, working together, we will help you
locate the problem and, chances are, correct it yourself...over the telephone without a service
call.
When more extensive service is necessary, we will assist you with direct factory trained
technicians or a qualified service organization for on-the-spot repair. If your service need is
covered by the warranty, we will arrange for the unit to be repaired at our expense and to your
satisfaction.
Regardless of your needs, our professional telephone technicians are available to assist you
Monday through Friday from 8:00 a.m. to 6:00 p.m. Eastern Time. Please contact us by
telephone or fax. If you wish to write, our mailing address is:
Thermo Fisher Scientific (Asheville) LLC
401 Millcreek Road, Box 649
Marietta, OH 45750
International customers, please contact your local Thermo Scientific distributor.

Cryobiological Storage Vessel vThermo Scientific
Table of Contents
Safety Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Please Read . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
General Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
easuring Liquid Nitrogen Quantity . . . . . . . . . . . . . . . . . . . . . . . . 3-2
Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
Warranty Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
Section 1
Section 2
Section 3
Section 4
Section 5

Cryobiological Storage Vessel 1-1Thermo Scientific
Please Read
Section 1 Safety Information
This manual contains important operating and safety information. The
user must carefully read and understand the contents of this manual prior
to the use of this equipment.
Your Thermo Scientific cryogenic vessel has been designed with function,
reliability, and safety in mind. It is the user’s responsibility to install it in
conformance with local electrical codes. For safe operation, pay attention
to the warnings and cautions throughout the manual.
All cryopreservation vessels, regardless of who manufactures them, use a
vacuum to provide for the super insulative properties needed to keep
nitrogen in a liquid form. They are very similar, in fact, to the thermos
vessels you may have used to store coffee, soup or milk.
Remember how fragile they were? Since no vacuum is perfect or will last
forever, we suggest that you monitor the consumption of liquid nitrogen
used by your vessel on a regular basis, i.e. every 1-2 days. By monitoring,
you may be able to anticipate subtle changes in consumption and possibly
react to problems before they arise. You may opt to use a level monitor for
this purpose.
Also, if your samples are super-critical, one-of-a-kind, or irreplaceable,
consider allocating your samples into 2 separate vessels.
Remember the old adage about placing all your eggs into one basket…
Finally, because no vacuum will last forever, consider having your vessel
tested by a qualified cryovessel manufacturer every 7-10 years regardless of
any problems (or lack thereof) you may have had in the past. This will
help insure your samples against sudden loss of nitrogen due to vacuum
failure.

1-2 Cryobiological Storage Vessel Thermo Scientific
Section 1
Safety Information
Warnings
• This unit is intended to be used by trained personnel in a well-
ventilated environment.
• Liquid nitrogen is extremely cold; it boils at -196°C.
• Follow all necessary precautions and warnings relevant to applicable
hazards such as frostbite, contact burn, asphyxiation, etc. in cryogenic
areas.
• To avoid injury due to frostbite or ruptured vials, use extreme care
whenever handling liquid nitrogen, liquid nitrogen storage or transfer
vessels or any objects which have come in contact with liquid
nitrogen.
• Leave no areas of skin exposed.
• Wear adequate PPE where necessary: face shield, cryogenic gloves,
cryogenic apron.
• Only use the power supply provided with the unit.
• Do not tightly seal liquid nitrogen containers or prevent nitrogen gas
from escaping.
• Always handle vials with tongs.
• Use extreme care to prevent spilling and splashing liquid nitrogen
during transfer and removal of storage contents and holders.
• Immediately remove any clothing or safety attire on which liquid
nitrogen has been spilled or splashed .
• Get immediate medical attention for any frostbite injuries due to
liquid nitrogen.
• Never overfill liquid nitrogen vessels.
When inser ing or removing cans, be careful no o come in con ac
wi h he neck ube area of he vessel. Remove or inser cans slowly,
in a ver ical manner. Scra ches on he neck ube can cause prema ure
vacuum failure.
Do no amper wi h, or remove vacuum por (covered by black
plas ic cap on side of vessel); his will void warran y.
Do not spill liquid nitrogen on vacuum port - this can cause vacuum
failure.
Always keep liquid nitrogen in an upright position.

Cryobiological Storage Vessel 2-1Thermo Scientific
Section 2 General Specifications
* Actual working time may vary due to current atmospheric conditions, container
history, manufacturing tolerances, and any individual patterns of use.
Environmen al Condi ions
Operating temperature: 17°C - 27°C
20% to 80% relative humidity, non-condensing.
Installation Category II (overvoltage) in accordance with IEC 664.
Pollution Degree 2 in accordance with IEC 664.
Altitude limit: 2,000 meters.
Storage: -25°C to 65°C; 10% to 85% relative humidity.
Locator and Locator Plus units are intended for indoor use only.
BioCane 20 BioCane 34 BioCane 47 BioCane 73
Height (Inches) 25.70" 26.60" 26.50" 27.00"
Diameter (Inches) 14.50" 18.20" 20.00" 22.00"
LN2 Capacity 20.50L 34.80L 47.40L 73.0L
Static Holding Time (Days) 227 193 121 121
Static LN2 Evaporation Rate* 0.1L/day 0.18L/day 0.40L/day 0.60L/day
Liquid Phase Capacity 1.2 ml 180 vials 720 vials 1512 vials 2016 vials

2-2 Cryobiological Storage Vessel Thermo Scientific
Section 2
General Specifications
Unpacking Before using your new vessel, carefully inspect it. Check for signs of
damage which may have occurred in shipment. It is advisable to fill (see
Filling Instructions) all new units with liquid nitrogen and watch liquid
nitrogen loss rate for a few days. If there are any problems, call Technical
Services as soon as possible.
The on-line warranty registration address is listed on the front of this
manual. The unit should be registered within 30 days to make warranty
effective. This information must include the serial number which is located
on the handle of the vessel.
Note The most prevalent cause of failure of liquid nitrogen storage vessels
is mechanical. The vessel neck tube supports the full weight of the inner
shell and all liquid nitrogen it contains. A side or corner blow to the vessel
causes the inner shell to swing in a pendulum motion causing the neck
tube to be damaged. Any storage vessel which has been exposed to an
accident, has been dropped or lowered to hit on one corner will tend to fail
more rapidly than one which has not.
Caution Exercise caution when moving your BioCane™ vessel. BioCane
cryobiological storage systems are not transportation vessels. Transport
carts are designed for mobility within the lab or lab to lab only. oving
full vessels long distances, over cracks in floor, thresholds, on inclined
ramps or in elevators can cause premature vacuum failure.
Note If samples must be transported under cryogenic conditions, consider
the Thermo Scientific Arctic Express line of Dry Shippers.

Cryobiological Storage Vessel 3-1Thermo Scientific
Section 3 Operation
To avoid damage to your cryogenic storage vessel which may result in
premature vacuum loss, it is important that the following procedure be
used during the addition of liquid nitrogen to a warm vessel:
1. Add only a small amount of liquid nitrogen (2-10 liters) to new or
warm vessels.
2. Allow this small amount of liquid nitrogen to sit in the covered vessel
for a minimum of 2 hours. This will limit stress caused by the sudden
temperature change associated with adding liquid nitrogen to a warm
vessel.
3. Add an additional 2-10 liters LN2 to vessel.
4. Allow vessel to sit for 48 hours and monitor liquid nitrogen
consumption.
5. Fill vessel as desired. Remember to allow for displacement of liquid
nitrogen when cannisters and canes are inserted.
6. Insert and remove cannisters slowly. Allow liquid nitrogen to run out
of cannisters.
Caution Never overfill liquid nitrogen vessels.
When inser ing or removing cans, be careful no o come in con ac wi h
he neck ube area of he vessel. Remove or inser cans slowly, in a
ver ical manner. Scra ches on he neck ube area can cause prema ure
vacuum failure.
Do not spill liquid nitrogen on vacuum port - this can cause vacuum
failure. s

3-2 Cryobiological Storage Vessel Thermo Scientific
Section 3
peration
Measuring Liquid
Nitrogen Quantity
1. Use a wooden yardstick to measure liquid nitrogen level. Never use a
hollow tube or plastic dipstick to measure liquid nitrogen level.
2. Level will be indicated by frost line which develops when dipstick is
removed and waved in a back and forth motion away from the user.

Cryobiological Storage Vessel 4-1Thermo Scientific
Section 4 Replacement Parts
Part Number Description
AY509X13 Nalge Cyrovials 1.2ml, 500/case
AY509X12 Nalge Cryovials 2.0ml, 500/case
AY509X20 CryoCane (aluminum) - each cane holds 5 vials for storage in BioCane cannisters (48/case)
AY509X21 CryoSleeve enclosures - Clear plastic sleeve fits over CryoCane for added security during handling or storage (100/case)
CS509X17 Nalgene System 100-ampule box accommodates most internally threaded 1.2 and 2.0 ml vials
CS509X18 Nalgene System 100-ampule box accommodates most internally threaded 1.2 and 2.0 ml vials
AY509X32 Nalgene System 100 Cyrogenic Vials - 1 ml (500/case)
AY509X33 Nalgene System 100 Cryogenic Vials - 1.5 ml (500/case)
AY509X34 Nalgene System 100 Cryogenic Benchtop Vial Holder (1/package)
AY509X35 Nalgene System 100 Cryogenic Benchtop Vial Holder (1/package)
CryoCane coders - Aluminum identification tabs that slip over the end of a CryoCane to identify the cane written on for further
identification (100/case)
AY509X22 White
AY509X23 Yellow
AY509X24 Blue
AY509X25 Green
AY509X26 Red
Cryovial closure color coders - Plastic disks that fit into the top of Nalge Cryovials to identify individual (100/package)
AY509X27 White
AY509X28 Yellow
AY509X29 Blue
AY509X30 Green
AY509X31 Red

4-2 Cryobiological Storage Vessel Thermo Scientific
Section 4
Replacement Parts
Refer to the Specification Plate for the complete model number, serial
number, and series number when requesting service, replacement parts or
in any correspondence concerning this unit.
All parts listed herein may be ordered from the Thermo Scientific dealer
from whom you purchased this unit or can be obtained promptly from the
factory.When service or replacement parts are needed, check first with your
dealer. If the dealer cannot process your request, then contact our
Technical Services Department.
Prior to returning any materials, contact our Technical Services
Department for a “Return aterials Authorization” number (R A).
aterial returned without an R A number will be refused.
Ordering Procedures
Part Number Description
HR509X25 BioCane 20 (CK509X2) [807]) replacement canister
HR509X26 BioCane 34 (CK509X3 [808]) replacement canister
HR509X27 BioCane 47 (CK509X4 [809]) replacement canister
HR509X28 BioCane 73 (CK509X6 [820]) replacement canister

Cryobiological Storage Vessel 5-1Thermo Scientific
Section 5
Warranty Information
THERMO FISHER SCIENTIFIC STANDARD PRODUCT WARRANTY
(LN2Vacuum)
The Warranty Period starts two weeks from the date your equipment is shipped from our facility. This allows for shipping
time so the warranty will go into effect at approximately the same time your equipment is delivered. The warranty protec-
tion extends to any subsequent owner during the first year warranty period.
During the first year, component parts proven to be non-conforming in materials or workmanship will be repaired or replaced
at Thermo's expense, labor included. LN2Vacuum Integrity is covered for five years. Installation and calibration are not
covered by this warranty agreement. The Technical Services Department must be contacted for warranty determination and
direction prior to performance of any repairs. Expendable items, glass, filters and gaskets are excluded from this warranty.
Replacement or repair of components parts or equipment under this warranty shall not extend the warranty to either the
equipment or to the component part beyond the original warranty period. The Technical Services Department must give prior
approval for return of any components or equipment. At Thermo's option, all non-conforming parts must be returned to
Thermo postage paid and replacement parts are shipped FOB destination.
THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES, WHETHER WRITTEN, ORAL OR
IMPLIED. NO WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL APPLY. Thermo shall not be liable for any indirect or consequential damages including, without limitation, damages relating to lost
profits or loss of products.
Your local Thermo Sales Office is ready to help with comprehensive site preparation information before your equipment
arrives. Printed instruction manuals carefully detail equipment installation, operation and preventive maintenance.
If equipment service is required, please call your Technical Services Department at 1-800-438-4851 (USA and Canada) or
1-740-373-4763. We're ready to answer your questions on equipment warranty, operation, maintenance, service and
special application. Outside the USA, contact your local distributor for warranty information.
Rev. 0 5/10
ISO
9001
REGISTERED

thermoscientific.com
© 2014 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its
subsidiaries. Specifications, terms
and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative
for details.
Thermo Fisher Scientific (Asheville) LLC
401 Millcreek Road
Marietta, hio 45750
United States
A Thermo Fisher Scientific Brand

Visitez notre site en ligne pour enregistrer votre garantie
www.thermoscientific.com/labwarranty
Guide de l’utilisateur
BioCane™
Cryoconservateur biologique type CK50900
Mode d’emploi et liste des pièces LT509X17 (7000807) Rév. 8

Thermo Scientific
Préface
Cryoconservateur biologique i
N° de catalogue Numéro du modèle Nom
CK509X2 807 BioCane 20
CK509X3 808 BioCane 34
CK509X4 809 BioCane 47
CK509X6 820 BioCane 73

Thermo Scientificii Cryoconservateur biologique
Préface
Contains Parts and Assemblies
Susceptible to Damage by
Electrostatic Discharge (ESD)
CAUTION
ATTENTION
Contient des pièces et des
ensembles dont l’électricité
statique est susceptible de
provoquer des dommages.
Important Lisez attentivement ce manuel d'instruction. Le défaut de lire, comprendre et suivre les instructions
dans ce manuel peut entraîner des dommages et à l'unité, des blessures au personnel d'exploitation et la
performance des équipements pauvres.
Attention Tous les réglages internes et la maintenance doivent être effectués par du personnel qualifié.
avec
Attention Il est recommandé d'avoir ce navire Testé par le fabricant ou technicien qualifié de service
cryovessel tous les 7-10 ans, indépendamment de tout problème (ou l'absence de celui-ci) peut vous
avez eu et le piège. Cela aidera à assurer vos échantillons contre la perte soudaine de l'azote liquide en
raison de l'échec de vide.
Avertissement L'azote liquide peut provoquer l'asphyxie sans avertissement. Stocker et utiliser l'azote
liquide uniquement dans un endroit bien ventilé. Comme les évaporation du liquide, le gaz résultant
déplace l'air et la zone normale. (La vapeur nuageuse qui apparaît lorsque l'azote liquide est exposé à
l'air est une condensation d'humidité, pas le gaz lui-même. Le gaz émis est invisible.) Dans les zones fermées, des
quantités excessives de gaz d'azote réduit la concentration d'oxygène et peuvent entraîner et d'asphyxie. Parce que
de l'azote gazeux est incolore, inodore et sans saveur, il ne peut pas être détecté par les sens humains. Respirer
une atmosphère qui contient moins de 19,5% d'oxygène peut provoquer des vertiges et le résultat rapidement et
inconscience et la mort. Par conséquent, l'utilisation d'équipements de surveillance de l'oxygène est fortement
recommandé.
Avertissement Contact de l'azote liquide avec la peau ou les yeux avertissement peut causer (congélation)
des blessures graves.
Avertissement
Le contenu de ce manuel est pour information seulement fins. Le contenu et le produit qu'il décrit sont sujets à
changement sans préavis. Thermo Fisher Scientific fait aucune représentation ou garantie quant à ce manuel. En
aucun cas, Thermo sera tenu responsable pour: tout dommage, direct ou indirect, découlant de ou liée à
l'utilisation de ce manuel.
Utilisation prévue:
Les Thermo Fisher Scientific réservoirs de stockage cryogénique sont destinés à stocker des échantillons
biologiques à des températures cryogéniques. Ces produits sont constitués d'un récipient et d'un couvercle, avec
ou sans un moniteur de niveau.
Utilisation involontaire:
Ces produits ne sont pas destinés à être utilisés Dans l'environnement du patient.
Lorsque ce manuel est traduit dans d'autres langues, la version américaine Angleščina est obligatoire.
© 2010 Thermo Fisher Scientific. Tous droits réservés. Imprimé en U.S.A.
Ne pas rouler. garder toujours réservoir d'azote liquide dans une position verticale.
Faire preuve de prudence pour éviter de renverser et de projections.